Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,533 T→C 10.4% D1032D (GAT→GAC)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5330TC10.4% 261.2 / 5.4 117D1032D (GAT→GAC) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (55/46);  new base C (8/6);  total (64/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAG  >  USA300TCH1516_ALE/622393‑622673
                                                                                                                                            |                                                                                                                                            
gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                                                                                              <  1:1060806/141‑1 (MQ=255)
  cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTc                                                                                                                                            >  2:513573/1‑141 (MQ=255)
  cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGAAAGCGACTCAGAGTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                                                                                            >  4:267863/1‑141 (MQ=255)
   tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCg                                                                                                                                           <  2:275278/141‑1 (MQ=255)
     aGATAGCGACTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                         <  2:931692/141‑1 (MQ=255)
      gATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGAc                                                                                                                                        >  2:765536/1‑141 (MQ=255)
      gATAGCGACGCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                                        <  2:995931/141‑1 (MQ=255)
        tAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                      >  3:187078/1‑141 (MQ=255)
        tAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                      <  1:801160/141‑1 (MQ=255)
           cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                                   <  1:305329/141‑1 (MQ=255)
           cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                                   >  3:310620/1‑141 (MQ=255)
           cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                                   <  2:1066229/141‑1 (MQ=255)
                cagattcagatAGCGATTCAGATTCAGACTGCGACTCAGAGTCAGACAGCGATTTCGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                                                                              <  4:230006/141‑1 (MQ=11)
                cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                                                                              >  3:292945/1‑141 (MQ=255)
                  gaatcaggtAGAGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGat                                                                                                                            <  4:350470/138‑2 (MQ=14)
                     tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                                                                                         >  2:796058/1‑141 (MQ=255)
                      cagatAGCGATTCTGACTCAGACAGTGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACTCAGAGTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                                        >  4:142396/1‑141 (MQ=255)
                      cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                                        >  2:886871/1‑141 (MQ=255)
                      cagatAGCGACGCAGACTCAGACAGCGACTCAGATTCAGACAGCGATGCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACTGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                                        <  2:339013/141‑1 (MQ=14)
                        gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGac                                                                                                                      >  2:482200/1‑140 (MQ=255)
                          tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                                                                                    <  3:207784/141‑1 (MQ=255)
                          tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCCGACTCAGACAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                                                                                    <  2:462328/141‑1 (MQ=255)
                            gCGATTCAGACTCAGACAGCGATTCAGATTCAGATCGCGATTCGGACTCAGACAGCGATTCAGACTCAGCTAGCATCCCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAg                                                                                                                  <  1:270119/141‑1 (MQ=17)
                                ttCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                                                                              <  2:665043/141‑1 (MQ=255)
                                  cagactcagacAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACACCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCg                                                                                                            <  1:881703/141‑1 (MQ=255)
                                    gactcagacAGCGACTCTGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                                          <  2:899987/141‑1 (MQ=255)
                                       gcagacAGCGCATCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                                       <  2:691990/140‑1 (MQ=14)
                                        cagacAGCGACTCAGATTCAGATAGCGCTTAACACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                                      >  1:303961/1‑141 (MQ=17)
                                         agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGCTAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                                     <  2:10543/141‑1 (MQ=255)
                                            cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                  <  1:868106/141‑1 (MQ=255)
                                            cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                  <  1:326585/141‑1 (MQ=255)
                                              gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAg                                                                                                >  2:221584/1‑141 (MQ=255)
                                              gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                                >  2:923299/1‑141 (MQ=255)
                                               cGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                                               <  1:815213/141‑1 (MQ=255)
                                                gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                                              >  1:455769/1‑141 (MQ=255)
                                                gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                                              >  3:97678/1‑141 (MQ=255)
                                                gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                                              >  3:239652/1‑141 (MQ=255)
                                                  cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGCAATAGATAGCGAGTAGCACTCATCTAGCGACCCAGCTCCAGAAAGCGTTTCAGAGTCAGATAg                                                                                            >  3:153412/1‑141 (MQ=17)
                                                    cagattcagatAGCGACTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                                          <  2:388274/141‑1 (MQ=255)
                                                        ttcagatAGCGACTCAGACTCAGAACGCGATGCAGACTCAGAAAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                      <  2:566944/141‑1 (MQ=17)
                                                         tcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactca                                                                                     >  3:365378/1‑141 (MQ=255)
                                                         tcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCgactca                                                                                     >  4:66971/1‑141 (MQ=255)
                                                           agatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                                                   >  2:498556/1‑141 (MQ=255)
                                                            gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                  <  1:1014783/141‑1 (MQ=255)
                                                            gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                  >  1:22421/1‑141 (MQ=255)
                                                            gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                  >  1:52267/1‑141 (MQ=255)
                                                            gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                  >  3:275941/1‑141 (MQ=255)
                                                              tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                <  1:569744/141‑1 (MQ=255)
                                                              aaGCGATTCAGAATCAGAATCGGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACCGAGATTGATTCTTAGTTAGCgactcagactc                                                                                <  2:546065/140‑1 (MQ=14)
                                                                    ttCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                          >  4:333589/1‑141 (MQ=255)
                                                                    ttCGGAATCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                          <  2:530310/141‑1 (MQ=255)
                                                                     tCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt                                                                         >  1:951419/1‑141 (MQ=255)
                                                                      ccgACTCAGACAGCGATTCAGACTCCGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGACTCCGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                        <  2:593244/139‑1 (MQ=14)
                                                                      cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                        >  2:78149/1‑141 (MQ=255)
                                                                      cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                        <  1:1058635/141‑1 (MQ=255)
                                                                      cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                        >  1:1046806/1‑141 (MQ=255)
                                                                      cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                        <  3:112259/141‑1 (MQ=255)
                                                                          cTCAGACAGCGATTCAGACTCAGATAGCGAATCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattc                                                                    >  2:975204/1‑141 (MQ=255)
                                                                           tCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattca                                                                   >  3:454065/1‑141 (MQ=255)
                                                                           tCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattca                                                                   >  2:874202/1‑141 (MQ=255)
                                                                            cacaCAGCGATTCACACTCAGACAGCCACTCAGCCTCAGCCAGCGACTCAGATTCAGACAGCGACTCCGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag                                                                  <  2:954841/138‑1 (MQ=14)
                                                                              gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACACAGACAGAgattcagaa                                                                >  4:252413/1‑140 (MQ=255)
                                                                                cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc                                                              <  2:925528/141‑1 (MQ=255)
                                                                                 aGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATATCGACGCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattca                                                             >  1:758179/1‑141 (MQ=255)
                                                                                  gCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGattcagattcag                                                            >  2:112094/1‑141 (MQ=255)
                                                                                  gCGATTCAGACTCAGATAGAGAATCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGattcagattcag                                                            >  3:449400/1‑141 (MQ=255)
                                                                                    gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAc                                                          <  3:59559/141‑1 (MQ=255)
                                                                                    gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGGCTCAGACTCAGAGAGGGAttcagattcagaa                                                          >  2:822646/1‑140 (MQ=255)
                                                                                      ttCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAg                                                        <  1:743061/141‑1 (MQ=255)
                                                                                      ttCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAg                                                        >  1:1061552/1‑141 (MQ=255)
                                                                                      ttCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGACAg                                                        >  4:189639/1‑141 (MQ=255)
                                                                                        cAGACTCAGATACCCACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCg                                                      <  2:268759/141‑1 (MQ=17)
                                                                                            cTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGAAACAGAAAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTGAGACAGCgactc                                                  >  2:247558/1‑141 (MQ=17)
                                                                                              cAGATAGGAACTCTGACTCATACAGCGACTCAGTTTCAGATAGCGATTCGGACTCAGATAGCGACTCTGATTCAGAGAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcag                                                <  4:153412/141‑1 (MQ=17)
                                                                                                gATAGCGACGCAGACGCAGACAGCGATGAAGATGCAGAAAGCGAATCTGACTCAGACAGCGACGCAGATTCAGAAAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgactcagac                                              <  2:242746/141‑1 (MQ=11)
                                                                                                  tAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagacac                                            >  4:444621/1‑139 (MQ=255)
                                                                                                     cgtctcagactcagaCACAGACCGAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGActcagactcaga                                         <  2:995412/138‑1 (MQ=14)
                                                                                                         tcagactcagacAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGAAAGc                                     >  2:225921/1‑141 (MQ=255)
                                                                                                          cagactcagtcAGCGACTCAGATTCCGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCg                                    <  2:457622/141‑1 (MQ=11)
                                                                                                          cagactcagacagcgactcagactcagacagcgactCGGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCg                                    <  2:306729/141‑1 (MQ=14)
                                                                                                          cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCg                                    >  4:374488/1‑141 (MQ=255)
                                                                                                           agactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGa                                   <  2:1022323/141‑1 (MQ=255)
                                                                                                                cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATACCGACTCGGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAg                              <  3:137552/141‑1 (MQ=255)
                                                                                                                 agagagCGACTCAGATTCAGAGAGCGATGCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCGGa                             <  3:456685/141‑1 (MQ=255)
                                                                                                                  gacAGCCACTCAGATTCAGATAGCGATTCCGACTCAGAGAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCGGAt                            <  2:758600/141‑1 (MQ=255)
                                                                                                                    cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc                          >  2:801696/1‑141 (MQ=255)
                                                                                                                    cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAGTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc                          >  2:716641/1‑141 (MQ=255)
                                                                                                                     aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCg                         >  1:859193/1‑141 (MQ=255)
                                                                                                                     aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCg                         >  1:975114/1‑141 (MQ=255)
                                                                                                                      gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGGCAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg                        <  3:432067/141‑1 (MQ=255)
                                                                                                                           tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGc                   >  3:308256/1‑141 (MQ=255)
                                                                                                                            cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCg                  >  1:788268/1‑141 (MQ=255)
                                                                                                                            cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCg                  >  4:59511/1‑141 (MQ=255)
                                                                                                                            cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAgaa                  >  2:1053028/1‑139 (MQ=255)
                                                                                                                              gattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGAc                <  1:498556/141‑1 (MQ=255)
                                                                                                                                ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTc              >  3:83806/1‑141 (MQ=255)
                                                                                                                                   agacaGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACACACATTCAGACAGTGACACAGa           >  2:238917/5‑141 (MQ=11)
                                                                                                                                        gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg      >  3:184079/1‑141 (MQ=255)
                                                                                                                                         cGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGa     <  4:310620/141‑1 (MQ=255)
                                                                                                                                            ttCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg  <  1:344560/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAG  >  USA300TCH1516_ALE/622393‑622673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑