BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	657,806	A→T	7.6%	A74A (GCA→GCT)	USA300HOU_RS03150 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	657,806	0	A	T	7.6%	339.5 / 11.4	115	A74A (GCA→GCT)	USA300HOU_RS03150	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (47/58); new base T (8/2); total (55/60)
Fisher's exact test for biased strand distribution p-value = 4.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAATTAAAAA  >  USA300TCH1516_ALE/657667‑657946

taataaGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAg                                                                                                                                             <  1:985606/141‑1 (MQ=255)

      gCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTAtct                                                                                                                                       <  3:287074/141‑1 (MQ=255)

       cACTTGGATTAGTTTATTTTTTTTTTTTTTTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGTTAtctt                                                                                                                                      >  4:32727/1‑140 (MQ=255)

        aCTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATctct                                                                                                                                     <  3:356393/141‑1 (MQ=255)

        aCTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTACTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATctct                                                                                                                                     <  4:327482/141‑1 (MQ=255)

          ttGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTTCAGGTATCTCTAc                                                                                                                                   >  4:107580/1‑141 (MQ=255)

           tGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACt                                                                                                                                  >  3:281570/1‑141 (MQ=255)

           tGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACt                                                                                                                                  <  1:807792/141‑1 (MQ=255)

           tGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACt                                                                                                                                  <  4:273795/141‑1 (MQ=255)

           tGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACt                                                                                                                                  <  4:332296/141‑1 (MQ=255)

                tAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTAtt                                                                                                                             >  1:292290/1‑141 (MQ=255)

                 aGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTc                                                                                                                            >  4:330267/1‑141 (MQ=255)

                 aGTTTATTTATTCCTCTTATTAATTGGATCAACCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAAACTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTc                                                                                                                            <  2:542795/141‑1 (MQ=17)

                   tttATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGc                                                                                                                          >  1:557890/1‑141 (MQ=255)

                   tttATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGc                                                                                                                          >  1:557917/1‑141 (MQ=255)

                   tttATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGc                                                                                                                          <  3:77421/141‑1 (MQ=255)

                    ttATTTTTTTTTTTTTTTTTTAGGTTCATCCCCAAAAGATTATTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAATCGTGTTAATAAGTTTGTTTTTTTTTTATTTTCTGTTATCTCTACTTTATTCgta                                                                                                                         >  4:205675/1‑139 (MQ=9)

                       ttttttCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGtt                                                                                                                      >  1:690786/1‑141 (MQ=255)

                        tttttCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTa                                                                                                                     >  1:1077029/1‑141 (MQ=255)

                         ttttCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTAc                                                                                                                    <  2:38626/141‑1 (MQ=255)

                         ttttCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTAc                                                                                                                    <  2:714293/141‑1 (MQ=255)

                            tttttttttTAATTGGTTAATCCCCAAAAGATACGTCAAAATTAGTAGTGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAATTATAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGTAGTTACAtt                                                                                                                 >  2:1072261/4‑141 (MQ=18)

                              cTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTaa                                                                                                               <  1:428869/141‑1 (MQ=255)

                              cTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTaa                                                                                                               <  2:984283/141‑1 (MQ=255)

                                tttATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGa                                                                                                             <  1:305507/141‑1 (MQ=255)

                                 tttttaATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTTGAGAAGAGTTTGAATATTTAAATATTTTGATTTTTTTTTTTTTTAATGTTTTTTGTCCATTTTCAGTTATCTCTACTTTATTCGCAGTTACATTAAGaa                                                                                                            >  4:365731/4‑141 (MQ=255)

                                  tattaATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTGTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGaaa                                                                                                           <  3:420528/141‑1 (MQ=255)

                                     taATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGta                                                                                                        >  3:173059/1‑141 (MQ=255)

                                        ttGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATac                                                                                                     >  4:58288/1‑141 (MQ=255)

                                        ttGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGTCTTGTTGCAGGTATCTCTACTTTATTTGCAGTTACATTAAGAAAGTATac                                                                                                     >  2:826652/1‑141 (MQ=255)

                                        ttGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATac                                                                                                     >  1:1051422/1‑141 (MQ=255)

                                         tGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAg                                                                                                                        <  1:93137/121‑1 (MQ=255)

                                         tGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAg                                                                                                                        >  2:93137/1‑121 (MQ=255)

                                         tGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATaca                                                                                                    <  2:523797/141‑1 (MQ=255)

                                         tGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATaca                                                                                                    >  3:257338/1‑141 (MQ=255)

                                          ggTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATacac                                                                                                   >  1:796443/1‑141 (MQ=255)

                                           gTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATAcaca                                                                                                  <  1:993422/141‑1 (MQ=255)

                                           gTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATAcaca                                                                                                  <  3:308607/141‑1 (MQ=255)

                                            ttCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATacgctt                                                                                                 >  1:823826/1‑137 (MQ=255)

                                             tCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTTTTTTTTTTCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACAtt                                                                                                >  2:303024/1‑141 (MQ=21)

                                               aTCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTa                                                                                              >  1:423001/1‑141 (MQ=255)

                                                tCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACGCTTTTAc                                                                                             <  2:823826/141‑1 (MQ=18)

                                                tCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTAc                                                                                             >  2:158859/1‑141 (MQ=255)

                                                   ccAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTTTACACATTTACTTg                                                                                          <  2:222546/141‑1 (MQ=255)

                                                   ccAAAAGATACGTCAAAACTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTg                                                                                          <  4:120365/141‑1 (MQ=255)

                                                    cAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGa                                                                                         >  2:1063434/1‑141 (MQ=255)

                                                    cAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGa                                                                                         >  3:143329/1‑141 (MQ=255)

                                                    cAAAAGATACGTAAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGa                                                                                         <  2:780642/141‑1 (MQ=255)

                                                       aaGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGacac                                                                                      <  2:312410/141‑1 (MQ=255)

                                                       aaGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGacac                                                                                      <  2:312421/141‑1 (MQ=255)

                                                       aaGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGacac                                                                                      <  1:842416/141‑1 (MQ=255)

                                                       aaGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGacac                                                                                      <  1:842400/141‑1 (MQ=255)

                                                        aGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACt                                                                                     <  2:456143/141‑1 (MQ=255)

                                                        aGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACt                                                                                     <  2:615034/141‑1 (MQ=255)

                                                        aGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACt                                                                                     <  1:118107/141‑1 (MQ=255)

                                                         gATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTc                                                                                    <  1:389463/141‑1 (MQ=255)

                                                          aTACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCa                                                                                   >  1:1086574/1‑141 (MQ=255)

                                                             cGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACt                                                                                <  1:1067487/141‑1 (MQ=255)

                                                             cGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACt                                                                                >  1:781031/1‑141 (MQ=255)

                                                                cAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGa                                                                             <  2:991457/141‑1 (MQ=255)

                                                                 aaaaTTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGAt                                                                            <  2:839549/141‑1 (MQ=255)

                                                                 aaaaTTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGAt                                                                            <  4:55401/141‑1 (MQ=255)

                                                                   aaTTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAg                                                                          >  3:168105/1‑141 (MQ=255)

                                                                       agtagAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACGCTTTTACTTGACACTCAACTTGATaaaaaa                                                                      <  3:318713/141‑1 (MQ=17)

                                                                       agtagAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGaaaa                                                                      >  3:376341/1‑141 (MQ=255)

                                                                           gagaAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAg                                                                  <  1:314675/141‑1 (MQ=255)

                                                                           gagaAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAg                                                                  >  2:380206/1‑141 (MQ=255)

                                                                            agaagaGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGTCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGt                                                                 >  4:233967/1‑141 (MQ=255)

                                                                                gagTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTAtt                                                             <  3:327506/141‑1 (MQ=255)

                                                                                  gTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGc                                                           <  1:394627/141‑1 (MQ=255)

                                                                                   tttGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCt                                                          <  4:376990/141‑1 (MQ=255)

                                                                                    ttGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCta                                                         >  4:271772/1‑141 (MQ=255)

                                                                                       aaaaTTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTTTTTTTTTTTATGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTatat                                                      >  2:195748/1‑141 (MQ=21)

                                                                                            ttAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATAtgttgt                                                 >  4:348829/1‑141 (MQ=255)

                                                                                            ttAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATAtgttgt                                                 >  2:658147/1‑141 (MQ=255)

                                                                                             tAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTa                                                <  2:557890/141‑1 (MQ=255)

                                                                                             tAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTa                                                <  2:557917/141‑1 (MQ=255)

                                                                                               aaTATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATc                                              >  4:258595/1‑141 (MQ=255)

                                                                                                 tatTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATcac                                            >  1:280814/1‑141 (MQ=255)

                                                                                                    tgtgAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACAtt                                         <  3:7014/141‑1 (MQ=255)

                                                                                                     gttaaaCCTGCTAAAAAGAATGTTTTTTTTCTTTTTTTTAGTATCTATACTTTATTCGCAGTTACATTAAGAAAGTATACGCTTTTACTTGACACTCAACTTGATAAAAAATTAGTTATTGCCATATGTTGCACCACAttt                                        >  4:202074/4‑141 (MQ=14)

                                                                                                      tgAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTta                                       <  1:977810/141‑1 (MQ=255)

                                                                                                         aaCCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAg                                    <  4:148010/141‑1 (MQ=255)

                                                                                                            cTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGa                                 <  4:43535/141‑1 (MQ=255)

                                                                                                             tGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGAt                                >  1:64176/1‑141 (MQ=255)

                                                                                                             tGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGAt                                >  1:64152/1‑141 (MQ=255)

                                                                                                               cTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTAATTTATTCGTAATTACACAAAGCAAGTACCAACATTTCCTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGAttt                              <  3:42371/141‑1 (MQ=18)

                                                                                                                 aaaaaGAATTTTTTTTGGGCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTa                            <  1:518492/141‑1 (MQ=255)

                                                                                                                  aaaaGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACGCTTTTACTTGACACTCAACTTGATAAAAAATTAGTTATTGCCATATGTTGCACCACATTTATAGGGATTTTaa                           >  1:297733/1‑141 (MQ=17)

                                                                                                                     aGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACAt                        <  1:634254/141‑1 (MQ=255)

                                                                                                                             ttttGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTa                >  1:192072/1‑141 (MQ=255)

                                                                                                                                 ggCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGcc            <  3:242199/141‑1 (MQ=255)

                                                                                                                                  gCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCt           >  2:1077914/1‑141 (MQ=255)

                                                                                                                                     aGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAAt        <  2:874376/141‑1 (MQ=255)

                                                                                                                                      gTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAAtt       <  3:78597/141‑1 (MQ=255)

                                                                                                                                      gTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAAtt       <  1:1072717/141‑1 (MQ=255)

                                                                                                                                        tGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAATTaa     <  2:129054/141‑1 (MQ=255)

                                                                                                                                           aGGTATCTCTACTTTATTCGCAGTTACATTAAGAGAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAATTaaaaa  >  2:667901/1‑141 (MQ=255)

                                                                                                                                           aGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAATTaaaaa  >  1:622572/1‑141 (MQ=255)

                                                                                                                                           aGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAATTaaaaa  >  2:32116/1‑141 (MQ=255)

TAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAATTAAAAA  >  USA300TCH1516_ALE/657667‑657946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`