Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 9,725 G→A 6.0% intergenic (+30/+57) gyrA → / ← nnrD DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE9,7250GA 6.0% 521.7 / 14.7 168intergenic (+30/+57)gyrA/nnrDDNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (82/75);  new base A (5/5);  total (87/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 176 total aligned reads displayed.

ACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGT  >  USA300TCH1516_ALE/9585‑9864
                                                                                                                                                   |                                                                                                                                            
aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAgggg                                                                                                                                              >  1:997634/1‑141 (MQ=255)
 cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATTGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGa                                                                                                                                             <  2:296796/141‑1 (MQ=255)
   gAAGTGAGTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGt                                                                                                                                           <  2:224369/141‑1 (MQ=255)
     aGTGATTTATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCt                                                                                                                                         <  4:109104/141‑1 (MQ=255)
     aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                                         <  1:432935/141‑1 (MQ=255)
       tgattgatTCAGAAGAAAATGATTAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTACATATAGGGAAGTCTTa                                                                                                                                       <  4:162995/141‑1 (MQ=255)
       tgattgatTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAt                                                                                                                                     <  1:107740/141‑1 (MQ=255)
            gattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAttttt                                                                                                                                 <  2:283433/141‑1 (MQ=255)
             attCATAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTa                                                                                                                                <  2:970307/141‑1 (MQ=255)
              ttCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTAt                                                                                                                               >  1:177309/1‑141 (MQ=255)
                cAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGc                                                                                                                             >  4:327271/1‑141 (MQ=255)
                 agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAttttttatta                                                                                                                            <  1:618374/141‑6 (MQ=38)
                   aagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGCGATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAAAAAGACTCCTCTCTAGGTCGCGGAGCCTTATTCTTATGCTAg                                                                                                                          >  1:562203/1‑141 (MQ=255)
                      aaaaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAGGAATAATAAAAAATAAGACTTCCCTACATGTAGGGGAGTCTTATTATTAAGCTAGaaa                                                                                                                       >  2:794063/1‑141 (MQ=255)
                        aaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGt                                                                                                                     <  3:305020/141‑1 (MQ=255)
                          tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                                   <  1:622781/141‑1 (MQ=255)
                          tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                                   <  4:30878/141‑1 (MQ=255)
                          tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                                   <  1:210884/141‑1 (MQ=255)
                          tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATA‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                                   >  1:243254/1‑141 (MQ=255)
                                  aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTAc                                                                                                           <  2:199616/141‑1 (MQ=255)
                                  aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTAc                                                                                                           <  4:444363/141‑1 (MQ=255)
                                    ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACta                                                                                                         >  1:192107/1‑141 (MQ=255)
                                    ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACta                                                                                                         >  1:746766/1‑141 (MQ=255)
                                       cGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTatat                                                                                                      >  1:311117/1‑141 (MQ=255)
                                        gTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATAtt                                                                                                     <  4:217761/141‑1 (MQ=255)
                                           ttGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCaa                                                                                                  >  3:47487/1‑141 (MQ=255)
                                            tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt                                                                                                 <  2:487767/141‑1 (MQ=255)
                                            tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt                                                                                                 <  2:293606/141‑1 (MQ=255)
                                                   aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt                                                                                          >  2:455014/1‑141 (MQ=255)
                                                    gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                                         >  1:100784/1‑141 (MQ=255)
                                                      cAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTGTACTATATTCAATGAt                                                                                              <  1:451709/141‑1 (MQ=255)
                                                        aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                                     <  2:940476/141‑1 (MQ=255)
                                                        aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                                     <  1:521773/141‑1 (MQ=255)
                                                        aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATATTTATGCTAGAAAGTAATGCTTTACTATATTAAATTATTAGTAAGGa                                                                                     >  4:190925/1‑141 (MQ=255)
                                                         gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc                                                                                    <  1:90786/141‑1 (MQ=255)
                                                             tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc                                                                                >  1:232149/1‑141 (MQ=255)
                                                              cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                                               >  3:53366/1‑141 (MQ=255)
                                                               aTGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACtt                                                                              <  3:199929/141‑1 (MQ=255)
                                                               aTGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACtt                                                                              <  3:374438/141‑1 (MQ=255)
                                                                tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                                             >  3:122668/1‑141 (MQ=255)
                                                                  gATCGTGTTGAAGAAGATATACAACAGGCATC‑‑‑‑‑‑AGATGAAGAATGATAAGAAATAAGACTGCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTGACTATATTCAATGATTAGTGATGACTAACTTTCt                                                                           <  2:997634/141‑1 (MQ=255)
                                                                  gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                                           <  3:330685/141‑1 (MQ=255)
                                                                  gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                                           >  1:1030712/1‑141 (MQ=255)
                                                                   aTCGTGTTGAAGAAGATATACAACAATCATG‑‑‑‑‑‑AGATGGAGAATAATAAAAAATAAGACTACCCTATATGAAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTa                                                                          >  2:913163/1‑141 (MQ=255)
                                                                      gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                                       >  1:127116/1‑141 (MQ=255)
                                                                      gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                                       <  1:671668/141‑1 (MQ=255)
                                                                       ggtTGAAGAAGATATACATCACTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg                                                                      <  4:352847/140‑1 (MQ=255)
                                                                          tGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGttt                                                                   >  1:489802/1‑141 (MQ=255)
                                                                                agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                                             >  4:60491/1‑141 (MQ=255)
                                                                                agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                                             >  4:336489/1‑141 (MQ=255)
                                                                                 gaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg                                                            <  2:686842/141‑1 (MQ=255)
                                                                                     taCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAg                                                        >  1:433023/1‑141 (MQ=255)
                                                                                      acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                                       <  2:185885/141‑1 (MQ=255)
                                                                                      acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                                       <  1:271121/141‑1 (MQ=255)
                                                                                      acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAACAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                                       <  1:1041746/141‑1 (MQ=255)
                                                                                       caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                      >  3:272818/1‑141 (MQ=255)
                                                                                       caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                      >  2:393441/1‑141 (MQ=255)
                                                                                       caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCCTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                      >  2:499878/1‑141 (MQ=255)
                                                                                       caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGGCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                      >  4:386869/1‑141 (MQ=255)
                                                                                         acaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt                                                    <  1:833678/141‑1 (MQ=255)
                                                                                         acaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt                                                    <  2:344300/141‑1 (MQ=255)
                                                                                             tcatc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                                <  2:1026836/141‑1 (MQ=255)
                                                                                             tcatc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                                >  2:666732/1‑141 (MQ=255)
                                                                                             tcatc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                                >  2:724733/1‑141 (MQ=255)
                                                                                              catc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCAt                                               <  3:451077/141‑1 (MQ=255)
                                                                                                 c‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                                            >  2:907586/1‑141 (MQ=255)
                                                                                                        acaTGAAGAATAATAAATAATAAGACTTCCCCATATGGAGGGCAGTCTTATTTGATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAGTGTTTCATTGCGTAAGGTATTTCATTGATa                                          <  3:327034/139‑1 (MQ=255)
                                                                                                        aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                                           >  2:125287/1‑141 (MQ=255)
                                                                                                        aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                                           <  1:323896/141‑1 (MQ=255)
                                                                                                            gaagaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGt                                       >  2:1067252/1‑141 (MQ=255)
                                                                                                             aagaaTAATAAAAAATAAGACGTCCCTATATGTAGTGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTc                                      <  4:415761/141‑1 (MQ=255)
                                                                                                              agaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt                                     <  1:840406/141‑1 (MQ=255)
                                                                                                                   aataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCTTTGCGTAAGGTATTTCATTGATAGGTCTTGAtg                                >  1:682025/1‑141 (MQ=255)
                                                                                                                    ataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                               >  1:728038/1‑141 (MQ=255)
                                                                                                                    ataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                               <  2:717268/141‑1 (MQ=255)
                                                                                                                       aaaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtg                             >  2:576217/1‑141 (MQ=255)
                                                                                                                        aaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtgg                            <  2:194244/141‑1 (MQ=255)
                                                                                                                         aaaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGc                           <  4:53366/141‑1 (MQ=255)
                                                                                                                          aaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTAATGGTTGca                          >  2:890190/1‑141 (MQ=255)
                                                                                                                           aaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac                         <  3:103367/141‑1 (MQ=255)
                                                                                                                             tAAGACTCCCCTACATATAGGGAAGTCTTATTTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcacc                        >  3:328910/1‑141 (MQ=38)
                                                                                                                               aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCacaaca                     >  2:804462/1‑141 (MQ=255)
                                                                                                                               aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca                     >  2:321883/1‑141 (MQ=255)
                                                                                                                                gACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacat                    >  2:455634/1‑141 (MQ=255)
                                                                                                                                 actccccTACATATAGGGAAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacata                   >  3:137706/5‑141 (MQ=255)
                                                                                                                                  cTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac                  <  2:635591/141‑1 (MQ=255)
                                                                                                                                    tCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat                >  1:256462/1‑141 (MQ=255)
                                                                                                                                    tCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat                >  1:698677/1‑141 (MQ=255)
                                                                                                                                     cccTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGTCACCAcatacata               >  3:408167/1‑141 (MQ=255)
                                                                                                                                     cccTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata               >  1:200707/1‑141 (MQ=255)
                                                                                                                                     cccTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata               >  4:2626/1‑141 (MQ=255)
                                                                                                                                        tacatatAGGGAAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCt            >  1:356358/7‑141 (MQ=255)
                                                                                                                                         atatGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt           >  1:185174/1‑141 (MQ=255)
                                                                                                                                         atatGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt           >  1:708208/1‑141 (MQ=255)
                                                                                                                                          tatGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCttt          <  1:132340/141‑1 (MQ=255)
                                                                                                                                            tGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTg        >  1:182175/1‑141 (MQ=255)
                                                                                                                                             gTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGc       >  4:203455/1‑141 (MQ=255)
                                                                                                                                             gTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGc       <  2:770392/141‑1 (MQ=255)
                                                                                                                                                ggggAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAg    >  2:750261/1‑141 (MQ=255)
                                                                                                                                                  ggAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGt  <  2:844236/141‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
ACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGT  >  USA300TCH1516_ALE/9585‑9864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: