Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 9,725 G→A 6.0% intergenic (+30/+57) gyrA → / ← nnrD DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE9,7250GA 6.0% 521.7 / 14.7 168intergenic (+30/+57)gyrA/nnrDDNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (82/75);  new base A (5/5);  total (87/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 176 total aligned reads displayed.

ACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGT  >  USA300TCH1516_ALE/9585‑9864
                                                                                                                                                   |                                                                                                                                            
aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAgggg                                                                                                                                              >  1:997634/1‑141 (MQ=255)
 cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATTGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGa                                                                                                                                             <  2:296796/141‑1 (MQ=255)
   gAAGTGAGTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGt                                                                                                                                           <  2:224369/141‑1 (MQ=255)
     aGTGATTTATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCt                                                                                                                                         <  4:109104/141‑1 (MQ=255)
     aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                                         <  1:432935/141‑1 (MQ=255)
       tgattgatTCAGAAGAAAATGATTAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTACATATAGGGAAGTCTTa                                                                                                                                       <  4:162995/141‑1 (MQ=255)
       tgattgatTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑‑AAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑t                                                                                                                                     <  1:107740/141‑1 (MQ=255)
            gattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑ttttt                                                                                                                                 <  2:283433/141‑1 (MQ=255)
             attCATAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTa                                                                                                                                <  2:970307/141‑1 (MQ=255)
              ttCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTAt                                                                                                                               >  1:177309/1‑141 (MQ=255)
                cAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGc                                                                                                                             >  4:327271/1‑141 (MQ=255)
                 agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCTTA‑ttttttatta                                                                                                                            <  1:618374/141‑6 (MQ=38)
                   aagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGCGATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAAAAAGACTCCTCTCTAGGTCGCGGAGCCTTA‑TTCTTATGCTAg                                                                                                                          >  1:562203/1‑141 (MQ=255)
                      aaaaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAGGAATAAT‑AAAAAATAAGACTTCCCTACATGTAGGGGAGTCTTA‑TTATTAAGCTAGaaa                                                                                                                       >  2:794063/1‑141 (MQ=255)
                        aaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGt                                                                                                                     <  3:305020/141‑1 (MQ=255)
                          tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa                                                                                                                   <  1:622781/141‑1 (MQ=255)
                          tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa                                                                                                                   <  4:30878/141‑1 (MQ=255)
                          tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa                                                                                                                   <  1:210884/141‑1 (MQ=255)
                          tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATA‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa                                                                                                                   >  1:243254/1‑141 (MQ=255)
                                  aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc                                                                                                           <  2:199616/141‑1 (MQ=255)
                                  aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc                                                                                                           <  4:444363/141‑1 (MQ=255)
                                    ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACta                                                                                                         >  1:192107/1‑141 (MQ=255)
                                    ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACta                                                                                                         >  1:746766/1‑141 (MQ=255)
                                       cGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTatat                                                                                                      >  1:311117/1‑141 (MQ=255)
                                        gTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATAtt                                                                                                     <  4:217761/141‑1 (MQ=255)
                                           ttGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCaa                                                                                                  >  3:47487/1‑141 (MQ=255)
                                            tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt                                                                                                 <  2:487767/141‑1 (MQ=255)
                                            tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt                                                                                                 <  2:293606/141‑1 (MQ=255)
                                                   aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt                                                                                          >  2:455014/1‑141 (MQ=255)
                                                    gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                                         >  1:100784/1‑141 (MQ=255)
                                                      cAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTGTACTATATTCAATGAt                                                                                              <  1:451709/141‑1 (MQ=255)
                                                        aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                                     <  2:940476/141‑1 (MQ=255)
                                                        aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                                     <  1:521773/141‑1 (MQ=255)
                                                        aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TATTTATGCTAGAAAGTAATGCTTTACTATATTAAATTATTAGTAAGGa                                                                                     >  4:190925/1‑141 (MQ=255)
                                                         gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc                                                                                    <  1:90786/141‑1 (MQ=255)
                                                             tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc                                                                                >  1:232149/1‑141 (MQ=255)
                                                              cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                                               >  3:53366/1‑141 (MQ=255)
                                                               aTGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACtt                                                                              <  3:199929/141‑1 (MQ=255)
                                                               aTGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACtt                                                                              <  3:374438/141‑1 (MQ=255)
                                                                tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                                             >  3:122668/1‑141 (MQ=255)
                                                                  gATCGTGTTGAAGAAGATATACAACAGGCATC‑‑‑‑‑‑AGATGAAGAATGAT‑AAGAAATAAGACTGCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTGACTATATTCAATGATTAGTGATGACTAACTTTCt                                                                           <  2:997634/141‑1 (MQ=255)
                                                                  gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                                           <  3:330685/141‑1 (MQ=255)
                                                                  gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                                           >  1:1030712/1‑141 (MQ=255)
                                                                   aTCGTGTTGAAGAAGATATACAACAATCATG‑‑‑‑‑‑AGATGGAGAATAAT‑AAAAAATAAGACTACCCTATATGAAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTa                                                                          >  2:913163/1‑141 (MQ=255)
                                                                      gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                                       >  1:127116/1‑141 (MQ=255)
                                                                      gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                                       <  1:671668/141‑1 (MQ=255)
                                                                       ggtTGAAGAAGATATACATCACTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg                                                                      <  4:352847/140‑1 (MQ=255)
                                                                          tGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGttt                                                                   >  1:489802/1‑141 (MQ=255)
                                                                                agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                                             >  4:60491/1‑141 (MQ=255)
                                                                                agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                                             >  4:336489/1‑141 (MQ=255)
                                                                                 gaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg                                                            <  2:686842/141‑1 (MQ=255)
                                                                                     taCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAg                                                        >  1:433023/1‑141 (MQ=255)
                                                                                      acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                                       <  2:185885/141‑1 (MQ=255)
                                                                                      acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                                       <  1:271121/141‑1 (MQ=255)
                                                                                      acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAACAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                                       <  1:1041746/141‑1 (MQ=255)
                                                                                       caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                      >  3:272818/1‑141 (MQ=255)
                                                                                       caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                      >  2:393441/1‑141 (MQ=255)
                                                                                       caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCCTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                      >  2:499878/1‑141 (MQ=255)
                                                                                       caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGGCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                      >  4:386869/1‑141 (MQ=255)
                                                                                         acaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt                                                    <  1:833678/141‑1 (MQ=255)
                                                                                         acaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt                                                    <  2:344300/141‑1 (MQ=255)
                                                                                             tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                                <  2:1026836/141‑1 (MQ=255)
                                                                                             tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                                >  2:666732/1‑141 (MQ=255)
                                                                                             tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                                >  2:724733/1‑141 (MQ=255)
                                                                                              catc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCAt                                               <  3:451077/141‑1 (MQ=255)
                                                                                                 c‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                                            >  2:907586/1‑141 (MQ=255)
                                                                                                        acaTGAAGAATAAT‑AAATAATAAGACTTCCCCATATGGAGGGCAGTCTTA‑‑TTTGATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAGTGTTTCATTGCGTAAGGTATTTCATTGATa                                          <  3:327034/139‑1 (MQ=255)
                                                                                                        aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                                           >  2:125287/1‑141 (MQ=255)
                                                                                                        aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                                           <  1:323896/141‑1 (MQ=255)
                                                                                                            gaagaaTAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGt                                       >  2:1067252/1‑141 (MQ=255)
                                                                                                             aagaaTAAT‑AAAAAATAAGACGTCCCTATATGTAGTGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTc                                      <  4:415761/141‑1 (MQ=255)
                                                                                                              agaaTAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt                                     <  1:840406/141‑1 (MQ=255)
                                                                                                                   aat‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCTTTGCGTAAGGTATTTCATTGATAGGTCTTGAtg                                >  1:682025/1‑141 (MQ=255)
                                                                                                                    at‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                               >  1:728038/1‑141 (MQ=255)
                                                                                                                    at‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                               <  2:717268/141‑1 (MQ=255)
                                                                                                                       aaaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtg                             >  2:576217/1‑141 (MQ=255)
                                                                                                                        aaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtgg                            <  2:194244/141‑1 (MQ=255)
                                                                                                                         aaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGc                           <  4:53366/141‑1 (MQ=255)
                                                                                                                          aaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTAATGGTTGca                          >  2:890190/1‑141 (MQ=255)
                                                                                                                           aaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac                         <  3:103367/141‑1 (MQ=255)
                                                                                                                             tAAGACTCCCCTACATATAGGGAAGTCTTATTTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcacc                        >  3:328910/1‑141 (MQ=38)
                                                                                                                               aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCacaaca                     >  2:804462/1‑141 (MQ=255)
                                                                                                                               aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca                     >  2:321883/1‑141 (MQ=255)
                                                                                                                                gACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacat                    >  2:455634/1‑141 (MQ=255)
                                                                                                                                 actccccTACATATAGGGAAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacata                   >  3:137706/5‑141 (MQ=255)
                                                                                                                                  cTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac                  <  2:635591/141‑1 (MQ=255)
                                                                                                                                    tCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat                >  1:256462/1‑141 (MQ=255)
                                                                                                                                    tCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat                >  1:698677/1‑141 (MQ=255)
                                                                                                                                     cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGTCACCAcatacata               >  3:408167/1‑141 (MQ=255)
                                                                                                                                     cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata               >  1:200707/1‑141 (MQ=255)
                                                                                                                                     cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata               >  4:2626/1‑141 (MQ=255)
                                                                                                                                        tacatatAGGGAAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCt            >  1:356358/7‑141 (MQ=255)
                                                                                                                                         atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt           >  1:185174/1‑141 (MQ=255)
                                                                                                                                         atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt           >  1:708208/1‑141 (MQ=255)
                                                                                                                                          tatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCttt          <  1:132340/141‑1 (MQ=255)
                                                                                                                                            tGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTg        >  1:182175/1‑141 (MQ=255)
                                                                                                                                             gTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGc       >  4:203455/1‑141 (MQ=255)
                                                                                                                                             gTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGc       <  2:770392/141‑1 (MQ=255)
                                                                                                                                                ggggAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAg    >  2:750261/1‑141 (MQ=255)
                                                                                                                                                  ggAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGt  <  2:844236/141‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
ACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGT  >  USA300TCH1516_ALE/9585‑9864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑