| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 895,207 | C→T | 6.0% | D76D (GAC→GAT) | metQ_2 → | Methionine‑binding lipoprotein MetQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 895,207 | 0 | C | T | 6.0% | 395.1 / 6.9 | 123 | D76D (GAC→GAT) | metQ_2 | Methionine‑binding lipoprotein MetQ |
| Reads supporting (aligned to +/- strand): ref base C (58/57); new base T (5/3); total (63/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
CAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGT > USA300TCH1516_ALE/895069‑895345 | cAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACaa < 1:1025308/141‑1 (MQ=255) gAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACAAAAAAAAAAAAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAgg < 4:33719/141‑1 (MQ=255) aaaaTAACGGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAGAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACCATTAACGATTACACTACACCTAATAAATTACTAGACGAAGGt < 2:509884/141‑1 (MQ=255) aTAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGaa > 3:76562/1‑141 (MQ=255) aaCAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAAt < 4:30417/141‑1 (MQ=255) aaCAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAAt > 2:56706/1‑141 (MQ=255) aaCAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAAt < 3:300013/141‑1 (MQ=255) aCAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAAtt > 3:186207/1‑141 (MQ=255) aGTAGGTGCTTCACCAGCACCACATGGTGAAATTTTAGAAAAAGCAAAACCATTATCAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGa > 4:24345/1‑141 (MQ=255) aGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGa > 4:110655/1‑141 (MQ=255) gTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGAc > 1:553908/1‑141 (MQ=255) aGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTGCACTACGCCTAATAAATTACTAGACAAAGGTGAAATTGACGc < 1:934254/141‑1 (MQ=255) aGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGc > 2:375263/1‑141 (MQ=255) aGGTGCTTCACCAGCACCACATGCCGAAATTTTAGAAAAAGCAAAACCAATAAAAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGc < 2:917992/141‑1 (MQ=255) tGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCaaa > 1:28181/1‑141 (MQ=255) tGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCaaa > 3:328421/1‑141 (MQ=255) cTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACt < 1:502612/141‑1 (MQ=255) ttCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTa > 2:871248/1‑141 (MQ=255) tggtgaaGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTa > 2:290776/7‑141 (MQ=255) cACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTAtt < 1:997443/141‑1 (MQ=255) cAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTcc < 3:427431/141‑1 (MQ=255) ccacATGCTGAAATATTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATGGACGCAAACTATTTCCAACAt > 2:662716/1‑141 (MQ=255) acaTGCTGGAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATac < 2:332968/141‑1 (MQ=255) aTGCTGANATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATacac < 2:812329/141‑1 (MQ=255) aTGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATacac > 2:390807/1‑141 (MQ=255) tGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACAcc < 1:990264/141‑1 (MQ=255) tGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACAcc < 2:917191/141‑1 (MQ=255) gAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCatat > 3:402945/1‑141 (MQ=255) aaaTTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGACCTAGATATTAAAACAATTAACGATTACACTACGCCTAATAAATTACTAGATAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATAtt < 1:651846/141‑1 (MQ=255) aaaTTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATAtt < 1:202330/141‑1 (MQ=255) aaaTTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATAtt > 3:441071/1‑141 (MQ=255) ttAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAac > 4:279567/1‑141 (MQ=255) ttAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAac < 4:200483/141‑1 (MQ=255) aGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAacac > 2:685196/1‑141 (MQ=255) gAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAAcaca > 1:394928/1‑141 (MQ=255) aaaaaGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACag < 3:308301/141‑1 (MQ=255) aaaaaGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACag < 3:88495/141‑1 (MQ=255) aaGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAgaga > 3:21271/1‑141 (MQ=255) cAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGaaaa < 1:387809/141‑1 (MQ=255) aaaCCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAg > 2:902916/1‑141 (MQ=255) aaCCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAgg > 1:718702/1‑141 (MQ=255) aaCCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAgg > 2:1069994/1‑141 (MQ=255) aCCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGa < 1:252818/141‑1 (MQ=255) ccATTATTAGAGAAAAAAGGTTATGACCTAGATATTAAAACAATTAACGATTACACTACGCCTAATAAATTACTAGATAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGAt > 4:158464/1‑141 (MQ=255) ttaGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGt < 3:270390/141‑1 (MQ=255) agagAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTa > 2:865/1‑141 (MQ=255) agaAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACa < 3:23794/141‑1 (MQ=255) agaAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACAATACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAACAAGGATCAAGGTTACa > 2:574318/1‑141 (MQ=255) aaaaGGTTATGAACTAGATATTAAAACAGTTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTACCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAAt < 1:72457/141‑1 (MQ=255) aGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGt < 1:865217/141‑1 (MQ=255) aGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAAAAAAAAAAAAAAGGATAAAGGTTACAAAATCGt < 4:380223/141‑1 (MQ=255) ttATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAg < 3:292475/141‑1 (MQ=255) ttATGAACGAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAg < 4:365035/141‑1 (MQ=255) tATGACCTAGATATTAAAACAATTAACGATTACACTACGCCTAATAAATTACTAGATAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGt > 3:164807/1‑141 (MQ=255) tATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAAGAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAAAAAAAAAAAAAAGGATAAAGGTTACAAAATCGTAAGt < 2:333815/141‑1 (MQ=255) tATGAACTAGATATTAAAACAATTAACGATAACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGt > 2:975331/1‑141 (MQ=255) tGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGc > 1:438386/1‑141 (MQ=255) tGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGc > 2:1055572/1‑141 (MQ=255) tGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGc > 2:315940/1‑141 (MQ=255) cTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGt < 3:145408/141‑1 (MQ=255) gATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGAt < 1:611499/141‑1 (MQ=255) atatTAACACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATg < 3:411292/141‑1 (MQ=255) tatTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGt < 4:369201/141‑1 (MQ=255) ttAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTc < 1:849316/141‑1 (MQ=255) ttAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTc < 1:510878/141‑1 (MQ=255) aaaaCAATTAACGATTACACTACGCCTAATAAATTACTAGATAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCAc > 4:32400/1‑141 (MQ=255) aaaCAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACt > 1:209585/1‑141 (MQ=255) aaaCAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACt < 2:493665/141‑1 (MQ=255) aCAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTa > 1:123474/1‑141 (MQ=255) cAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACAAAGAAAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAg < 4:271057/141‑1 (MQ=255) aaTTAACGATTACACTACACCTAATAAATTACTAGACAAAGGt > 2:162888/1‑43 (MQ=255) aTTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGaa > 4:132662/1‑141 (MQ=255) tAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAAcc > 2:503211/1‑141 (MQ=255) aCGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTa > 1:475794/1‑141 (MQ=255) gATTACACTACGCCTAATAAATTACTAGATAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATg > 3:140599/1‑141 (MQ=255) gATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATg < 1:724728/141‑1 (MQ=255) aTTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATgg < 1:1055572/141‑1 (MQ=255) aTTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATgg < 1:85251/141‑1 (MQ=255) acactacacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTg > 2:390344/1‑141 (MQ=255) actacacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGta < 1:386990/141‑1 (MQ=255) tacacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGTCGGTGATGTTCACTTAGAACCTATGGCTGtata < 1:564157/141‑1 (MQ=255) tacacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGtata < 1:325337/141‑1 (MQ=255) acacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTATAACCTATGGCTGTATAc < 3:449869/141‑1 (MQ=255) acacCTAATAAATTACTAGACAAAGGTGAAACTGACGCAAACTATTTCCAACATACACCATATTTAAAAACAGAGAAAAAGGAT‑AAGGTTACAACATCGTAAGTGCCGGTGATGTTCACTCAGCAACTATGGCTGTATAct > 2:702337/1‑141 (MQ=255) cacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATAct < 4:136514/141‑1 (MQ=255) cTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTa > 1:479557/1‑141 (MQ=255) cTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTa < 3:338600/141‑1 (MQ=255) taataaATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTaa > 1:241180/1‑141 (MQ=255) aataaATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTaaa > 1:276235/1‑141 (MQ=255) aataaATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTaaa > 1:142766/1‑141 (MQ=255) ataaATTACTAGATAAAGGTAAAATTGACGCAAACTATTTCCCACATACCCCCTCTTTACCCGCATAGAAGCCGGACAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTaaaa < 1:376657/141‑1 (MQ=255) ataaATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTaaaa < 1:304657/141‑1 (MQ=255) ataaATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTaaaa < 2:391011/141‑1 (MQ=255) ataaATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTaaaa < 2:728545/141‑1 (MQ=255) taaATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTaaaaa > 2:931109/1‑141 (MQ=255) tACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGtata > 1:1004628/1‑141 (MQ=255) tACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGtata > 1:519887/1‑141 (MQ=255) aCTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATaa > 1:740015/1‑141 (MQ=255) aCTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAAAATAAAGGGTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATaa < 2:817786/141‑1 (MQ=255) gACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGt > 4:237010/1‑141 (MQ=255) | CAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGT > USA300TCH1516_ALE/895069‑895345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |