Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 950,093 T→A 5.6% intergenic (+87/‑275) yugI_2 → / → USA300HOU_RS04740 General stress protein 13/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE950,0930TA 5.6% 445.3 / 10.8 138intergenic (+87/‑275)yugI_2/USA300HOU_RS04740General stress protein 13/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (73/57);  new base A (3/5);  total (76/62)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 146 total aligned reads displayed.

GAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGT  >  USA300TCH1516_ALE/949953‑950233
                                                                                                                                            |                                                                                                                                              
gAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAt                                                                                                                                                <  2:770514/141‑1 (MQ=255)
  ccGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttt                                                                                                                                              >  2:520309/2‑141 (MQ=255)
  aCGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttt                                                                                                                                              >  3:384373/1‑141 (MQ=255)
    gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttatt                                                                                                                                            >  3:157783/1‑138 (MQ=255)
    gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTtta                                                                                                                                            >  2:91140/1‑141 (MQ=255)
    gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTtta                                                                                                                                            >  1:143668/1‑141 (MQ=255)
      ttACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatt                                                                                                                                          >  3:20115/1‑141 (MQ=255)
      ttACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatt                                                                                                                                          >  1:1012550/1‑141 (MQ=255)
       tACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAGAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatta                                                                                                                                         >  1:692197/1‑141 (MQ=255)
          cAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGAt                                                                                                                                      <  4:349218/141‑1 (MQ=255)
               tGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTaa                                                                                                                                 <  1:439062/141‑1 (MQ=255)
               tGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTaa                                                                                                                                 <  1:418807/141‑1 (MQ=255)
                 gATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtc                                                                                                                               >  3:88767/1‑141 (MQ=255)
                   tAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtctc                                                                                                                             >  2:219861/1‑141 (MQ=255)
                    aaaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                            <  1:122163/141‑1 (MQ=255)
                    aaaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                            <  2:390146/141‑1 (MQ=255)
                      aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt                                                                                                                          >  3:86460/1‑141 (MQ=255)
                       aCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCtttt                                                                                                                         <  2:769704/141‑1 (MQ=255)
                         attCAAAGCGAGCAATGCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g                                                                                                                     <  2:124506/139‑1 (MQ=255)
                         aGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g                                                                                                                     >  1:656900/1‑141 (MQ=255)
                         aGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g                                                                                                                     <  1:712250/141‑1 (MQ=255)
                          gTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑Gt                                                                                                                    >  2:594774/1‑141 (MQ=255)
                           tCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTc                                                                                                                   <  1:33392/141‑1 (MQ=255)
                             aaaGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGACTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTa                                                                                                                 >  2:443723/1‑141 (MQ=255)
                                  gAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATa                                                                                                            <  2:870245/141‑1 (MQ=255)
                                  gAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATa                                                                                                            >  1:287068/1‑141 (MQ=255)
                                    gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                          >  3:404276/1‑141 (MQ=255)
                                    gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                          >  4:144825/1‑141 (MQ=255)
                                    gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                          >  1:303422/1‑141 (MQ=255)
                                    gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                          <  2:219773/141‑1 (MQ=255)
                                    gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                          >  3:366989/1‑141 (MQ=255)
                                      aaTTCGAAACGACTAAAGGACCAGATAAATCGTACCGAAAAACATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt                                                                                                        <  1:597274/141‑1 (MQ=255)
                                      aaTTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt                                                                                                        >  2:430869/1‑141 (MQ=255)
                                      aaTTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt                                                                                                        <  1:91140/141‑1 (MQ=255)
                                        ttCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTaa                                                                                                      >  2:238286/1‑141 (MQ=255)
                                         tCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAat                                                                                                     >  2:621314/1‑141 (MQ=255)
                                            aaaCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtg                                                                                                  <  1:104037/141‑1 (MQ=255)
                                             aaCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtga                                                                                                 >  2:503699/1‑141 (MQ=255)
                                              aCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtgat                                                                                                >  3:285613/1‑141 (MQ=255)
                                                   tAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATgattgatt                                                                                           <  1:643000/141‑1 (MQ=255)
                                                    aaaGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTc                                                                                          <  1:808900/141‑1 (MQ=255)
                                                       ggAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTAt                                                                                       >  1:712956/1‑141 (MQ=255)
                                                          aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTTTACGATAACGTAATATGATTGATTCTATTTa                                                                                    >  2:44694/1‑141 (MQ=255)
                                                          aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGAATGATTCTATTTa                                                                                    >  4:367281/1‑141 (MQ=255)
                                                          aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑G‑ATCTACGATAACGTAATATGATTGATTCTATTTa                                                                                    >  2:602685/1‑141 (MQ=255)
                                                           cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGGAATGAAAGTTTCTTAGGCTATAAAAGAGGTTAGTATTTATTAAATGTTAGTATATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                   >  3:42567/1‑141 (MQ=255)
                                                           cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                   >  4:200325/1‑141 (MQ=255)
                                                           cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                   <  3:249557/141‑1 (MQ=255)
                                                           cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                   <  1:206173/141‑1 (MQ=255)
                                                              aTAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACg                                                                                >  1:10211/1‑141 (MQ=255)
                                                                      tACCGAAAATCATACATATTGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg                                                                        <  3:136246/141‑1 (MQ=255)
                                                                      tACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg                                                                        >  2:1030898/1‑141 (MQ=255)
                                                                       aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTTTTTTAACGTAATATGATTGATTCTATTTACACTTACAAATgg                                                                       >  4:417071/1‑141 (MQ=255)
                                                                       aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAACTgg                                                                       >  2:807678/1‑141 (MQ=255)
                                                                       aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATgg                                                                       >  4:173339/1‑141 (MQ=255)
                                                                       aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATgg                                                                       >  1:994858/1‑141 (MQ=255)
                                                                        ccGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGt                                                                      <  2:333389/141‑1 (MQ=255)
                                                                         cGAAAATCATACAAAGGGTGTGAAATGAAAGTTTCTTAGACTATAAAAGGGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGtt                                                                     <  2:935344/141‑1 (MQ=255)
                                                                           aaaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTa                                                                   >  1:301794/1‑141 (MQ=255)
                                                                            aaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTGT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTaa                                                                  >  3:18537/1‑141 (MQ=255)
                                                                                  tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                            >  2:845946/1‑79 (MQ=255)
                                                                                  tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc                                                            <  1:883703/141‑1 (MQ=255)
                                                                                   aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACa                                                           >  2:63508/1‑141 (MQ=255)
                                                                                   aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATCTACACGTACAAATGGTTTAAGGTGACa                                                           >  2:1011674/1‑141 (MQ=255)
                                                                                    cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat                                                          <  2:1041038/141‑1 (MQ=255)
                                                                                    cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat                                                          >  2:560165/1‑141 (MQ=255)
                                                                                        gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                      >  1:389866/1‑141 (MQ=255)
                                                                                        gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                      <  1:519489/141‑1 (MQ=255)
                                                                                        gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                      <  3:211416/141‑1 (MQ=255)
                                                                                          gTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAt                                                    <  4:71101/141‑1 (MQ=255)
                                                                                           tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                   <  3:161475/141‑1 (MQ=255)
                                                                                           tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                   >  2:290685/1‑141 (MQ=255)
                                                                                            cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa                                                  >  1:249847/1‑141 (MQ=255)
                                                                                              gaaatgaaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATc                                                >  2:958084/1‑141 (MQ=255)
                                                                                               aaatgaaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCt                                               >  2:824612/1‑141 (MQ=255)
                                                                                                    aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt                                          <  1:634127/141‑1 (MQ=255)
                                                                                                       gTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTGTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtaga                                       <  1:427531/141‑1 (MQ=255)
                                                                                                        tttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagat                                      <  1:594878/141‑1 (MQ=255)
                                                                                                        tttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagat                                      >  2:951778/1‑141 (MQ=255)
                                                                                                         ttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagata                                     >  2:90030/1‑141 (MQ=255)
                                                                                                             tAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAAt                                 >  3:356217/1‑141 (MQ=255)
                                                                                                               gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCg                               <  4:180139/141‑1 (MQ=255)
                                                                                                                 cTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATTCATTATCTTTGTTAGATAGAATCGtt                             >  2:278320/1‑141 (MQ=255)
                                                                                                                 cTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGtt                             <  2:272938/141‑1 (MQ=255)
                                                                                                                  acaaaaaGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCTTTTATAGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGt                              >  1:81670/4‑141 (MQ=25)
                                                                                                                   ataAAAGCGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTCAAACAGGTTTAACGTGATATCTCTATTCTCTTTGTTAGATAGAATCGTTGa                           <  4:42567/141‑1 (MQ=255)
                                                                                                                        agagATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGc                      <  2:217245/141‑1 (MQ=255)
                                                                                                                           gaTTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAat                   <  2:143668/141‑1 (MQ=255)
                                                                                                                             ttAGTATCTAATAAAATTTAATAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAatat                 >  2:490241/1‑141 (MQ=255)
                                                                                                                              tAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATAtt                >  3:420955/1‑141 (MQ=255)
                                                                                                                               aGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTg               >  4:76049/1‑141 (MQ=255)
                                                                                                                                gTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt              <  4:297315/141‑1 (MQ=255)
                                                                                                                                gTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt              >  1:192089/1‑141 (MQ=255)
                                                                                                                                      aTTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTgg        <  1:33427/141‑1 (MQ=255)
                                                                                                                                       ttAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGa       <  2:684360/141‑1 (MQ=255)
                                                                                                                                         aaaTTTTATTAGATACTAATCTCTTTT‑T‑TTTTTTTTTTTTATAATATGATTGATTTTATTTATAAGTACAATTGGTTTAGGGTAACATATCTATTATTTTGGTTAGATAGAATCTTTGATTTTCATTATTGAATGTGTAtt     >  2:837673/1‑141 (MQ=255)
                                                                                                                                         aaaTTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt     >  4:229379/1‑141 (MQ=255)
                                                                                                                                         aaaTTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt     <  4:29342/141‑1 (MQ=255)
                                                                                                                                         aaaTTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt     >  2:189212/1‑141 (MQ=255)
                                                                                                                                            ttttATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGt  >  1:661310/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                              
GAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGT  >  USA300TCH1516_ALE/949953‑950233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑