Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 985,069 A→T 5.6% intergenic (‑134/‑161) USA300HOU_RS04870 ← / → fabH hypothetical protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE985,0690AT 5.6% 393.3 / 11.3 124intergenic (‑134/‑161)USA300HOU_RS04870/fabHhypothetical protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase 3
Reads supporting (aligned to +/- strand):  ref base A (60/57);  new base T (4/3);  total (64/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 130 total aligned reads displayed.

GTTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAATTT  >  USA300TCH1516_ALE/984929‑985209
                                                                                                                                            |                                                                                                                                            
gtTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTaaa                                                                                                                                              >  2:1074593/1‑141 (MQ=255)
 tttCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAAt                                                                                                                                             >  4:308563/1‑141 (MQ=255)
  ttCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAAtt                                                                                                                                            <  4:244774/141‑1 (MQ=255)
    cATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTaa                                                                                                                                          >  1:69784/1‑141 (MQ=255)
       gTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAgcg                                                                                                                                       <  4:122770/141‑1 (MQ=255)
         cATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCa                                                                                                                                     >  2:684279/1‑141 (MQ=255)
         cATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCa                                                                                                                                     <  1:185184/141‑1 (MQ=255)
          aTTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCAt                                                                                                                                    <  1:570883/141‑1 (MQ=255)
            tCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTa                                                                                                                                  >  1:399057/1‑141 (MQ=255)
                ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTaaagc                                                                                                                              >  1:391161/1‑139 (MQ=255)
                ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAc                                                                                                                              >  1:388776/1‑141 (MQ=255)
                ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAc                                                                                                                              >  1:337538/1‑141 (MQ=255)
                 cTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAcc                                                                                                                             <  2:719926/141‑1 (MQ=255)
                      cTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAAttt                                                                                                                        >  1:966606/1‑141 (MQ=255)
                            ttATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACt                                                                                                                  <  1:522745/141‑1 (MQ=255)
                             tatatataTTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTGTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTg                                                                                                                 <  3:375187/141‑1 (MQ=255)
                               tttatatTTTATTTGAAGTGAAAGTTTGAAATTTGTATAGTAACATCTCATATATTTTGACCTTATTATTCAGTTTTAATAAATGATTTTATCTTAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGat                                                                                                               <  2:724159/139‑2 (MQ=255)
                               tatatatTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGaa                                                                                                               <  1:1012059/141‑1 (MQ=255)
                                   tattttatttTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAGATTAAGCGCATTAAAGCCAATTTCATACTGAAAttt                                                                                                           <  2:291195/141‑1 (MQ=255)
                                      tttattttaAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGAc                                                                                                        <  1:36176/141‑1 (MQ=255)
                                       ttattttaAGTGAAAGTTAGAAATTTGTATAGTAACATCTAATATATTTTGACCATATTATAGAGTTTAACTAAATGATTTTATCTGAATGGCTATTATAAATTGCGCGCATTAAAACCACTTTCATACTGAAATTTGACg                                                                                                       <  2:500345/141‑1 (MQ=255)
                                       ttattttaAGTGAAAGTTAGAAAATTGTATAGCAACATCTTATATATTTAGATGATACTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACg                                                                                                       <  1:1024935/141‑1 (MQ=255)
                                         attttaAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGat                                                                                                     <  3:411070/141‑1 (MQ=255)
                                               aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                               >  2:734452/1‑141 (MQ=255)
                                               aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                               >  1:888025/1‑141 (MQ=255)
                                               aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                               >  2:236051/1‑141 (MQ=255)
                                                    aaGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCAtt                                                                                          >  1:99610/1‑141 (MQ=255)
                                                    aaGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCAtt                                                                                          <  3:323270/141‑1 (MQ=255)
                                                     aGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTa                                                                                         >  4:134979/1‑141 (MQ=255)
                                                      gTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaa                                                                                        >  4:261413/1‑141 (MQ=255)
                                                      gTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaa                                                                                        >  1:174001/1‑141 (MQ=255)
                                                      gTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaa                                                                                        <  2:829989/141‑1 (MQ=255)
                                                       ttAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaaa                                                                                       >  1:609298/1‑141 (MQ=255)
                                                        tAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaaaa                                                                                      <  4:362996/141‑1 (MQ=255)
                                                        tAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaaaa                                                                                      <  4:184494/141‑1 (MQ=255)
                                                         aGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAt                                                                                     >  2:317137/1‑141 (MQ=255)
                                                         aGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAt                                                                                     >  1:538361/1‑141 (MQ=255)
                                                          gAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAtt                                                                                    <  1:127921/141‑1 (MQ=255)
                                                          gAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAtt                                                                                    >  2:10429/1‑141 (MQ=255)
                                                          gAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAtt                                                                                    >  4:283926/1‑141 (MQ=255)
                                                             aTTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTtta                                                                                 >  1:1045392/1‑141 (MQ=255)
                                                             aTTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTtta                                                                                 >  2:115767/1‑141 (MQ=255)
                                                              tttGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttat                                                                                <  1:1074593/141‑1 (MQ=255)
                                                               ttGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttatt                                                                               >  1:162250/1‑141 (MQ=255)
                                                                tGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttatta                                                                              <  2:696868/141‑1 (MQ=255)
                                                                tGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttatta                                                                              >  2:172757/1‑141 (MQ=255)
                                                                tGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttatta                                                                              >  4:257077/1‑141 (MQ=255)
                                                                 gTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTaa                                                                             >  2:907418/1‑141 (MQ=255)
                                                                  tataGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAAc                                                                            <  1:375670/141‑1 (MQ=255)
                                                                     aGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGTATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAg                                                                         <  2:286802/141‑1 (MQ=255)
                                                                      gTAACATCTCATATATTTTGACCATATTATACAGTTTAACTAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGt                                                                        <  1:799932/141‑1 (MQ=255)
                                                                      gTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGt                                                                        >  2:621054/1‑141 (MQ=255)
                                                                         aCATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCaa                                                                     <  1:172757/141‑1 (MQ=255)
                                                                                    atTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACct                                                          <  2:396365/141‑1 (MQ=255)
                                                                                     ttttGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACctt                                                         <  1:1030670/141‑2 (MQ=255)
                                                                                     ttttGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACctc                                                         >  2:1018622/1‑141 (MQ=255)
                                                                                     ttttGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCGATTCTAAATTAAGCGCACTAAAACCAATTTCATACTGAAATTTGACGATATTAACGCATTAAATTTTTATTAACTAGTTAATATTcctccctc                                                         <  4:400825/141‑1 (MQ=255)
                                                                                       ttGATCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTg                                                       <  2:866851/141‑1 (MQ=255)
                                                                                             atatTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGa                                                 <  2:607920/141‑1 (MQ=255)
                                                                                             atatTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGa                                                 <  1:995026/141‑1 (MQ=255)
                                                                                              tattttACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCATTTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAg                                                >  2:287354/1‑141 (MQ=255)
                                                                                                ttatACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGtt                                              >  1:286453/1‑141 (MQ=255)
                                                                                                ttatACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGtt                                              <  4:105144/141‑1 (MQ=255)
                                                                                                 tataCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGttt                                             >  1:976615/1‑141 (MQ=255)
                                                                                                 tataCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGttt                                             <  1:960779/141‑1 (MQ=255)
                                                                                                  ataCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTa                                            >  3:416731/1‑141 (MQ=255)
                                                                                                    aCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTaaa                                          >  4:122649/1‑141 (MQ=255)
                                                                                                        tttAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGt                                      <  4:351131/141‑1 (MQ=255)
                                                                                                         ttAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTa                                     <  4:165497/141‑1 (MQ=255)
                                                                                                            aataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATc                                  <  1:940474/141‑1 (MQ=255)
                                                                                                            aataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATc                                  >  3:92615/1‑141 (MQ=255)
                                                                                                             ataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCt                                 >  1:490071/1‑141 (MQ=255)
                                                                                                                aatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATg                              <  3:226512/141‑1 (MQ=255)
                                                                                                                   gATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTTAGTTTAAAAAGTAATCTATGTTa                           >  2:477076/1‑141 (MQ=255)
                                                                                                                    aTTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTaa                          <  2:920297/141‑1 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTCTGTTAAAt                        >  1:126092/1‑141 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAt                        >  1:456779/1‑141 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAt                        >  4:33134/1‑141 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAt                        <  2:213236/141‑1 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGATTAAAAAGTAATCTATGTTAAAt                        <  2:451394/141‑1 (MQ=255)
                                                                                                                       ttATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAtt                       <  1:907418/141‑1 (MQ=255)
                                                                                                                          tCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAAt                    >  1:576105/1‑141 (MQ=255)
                                                                                                                           cTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATa                   >  3:304565/1‑141 (MQ=255)
                                                                                                                            tGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATAc                  >  1:719684/1‑141 (MQ=255)
                                                                                                                             gAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATAcc                 >  4:349793/1‑141 (MQ=255)
                                                                                                                             gAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATAcc                 <  1:794212/141‑1 (MQ=255)
                                                                                                                              aaTGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCt                >  1:286410/1‑141 (MQ=255)
                                                                                                                                  gCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTa            <  3:431910/141‑1 (MQ=255)
                                                                                                                                  gCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTa            <  4:151543/141‑1 (MQ=255)
                                                                                                                                  gCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTa            <  3:369280/141‑1 (MQ=255)
                                                                                                                                    tATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTAtt          >  4:444431/1‑141 (MQ=255)
                                                                                                                                    tATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTAtt          <  4:418810/141‑1 (MQ=255)
                                                                                                                                      ttCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTaa        >  1:377990/1‑141 (MQ=255)
                                                                                                                                       tCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTaaa       <  3:185630/141‑1 (MQ=255)
                                                                                                                                       tCTAAATTAAACGCATTAAAACCAACATCGTACTGAAATTTGACTATAATAATGCATTAGAATTTTATTAACTAGTCAATATTCGTATCTCTGACTTGAGATGAAAAAGTAAACTATGTTAAGTTGACCCCTGGTCTTacg       <  2:381992/141‑3 (MQ=255)
                                                                                                                                        cTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTaaaa      <  1:743552/141‑1 (MQ=255)
                                                                                                                                          aaaTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAAt    >  2:552095/1‑141 (MQ=255)
                                                                                                                                          aaaTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAAt    <  2:723535/141‑1 (MQ=255)
                                                                                                                                            aTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAAttt  >  3:388522/1‑141 (MQ=255)
                                                                                                                                            aTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACATGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAAttt  <  2:406460/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GTTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAATTT  >  USA300TCH1516_ALE/984929‑985209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: