BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,052,403	G→A	5.1%	D335D (GAC→GAT)	sspB ←	Staphopain B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,052,403	0	G	A	5.1%	379.3 / 4.8	119	D335D (GAC→GAT)	sspB	Staphopain B
Reads supporting (aligned to +/- strand): ref base G (42/70); new base A (3/4); total (45/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

AATGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATCAT  >  USA300TCH1516_ALE/1052269‑1052543

aaTGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCGTTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTa                                                                                                                                        >  1:199676/1‑141 (MQ=255)

aaTGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTa                                                                                                                                        >  1:689782/1‑141 (MQ=255)

aaTGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTa                                                                                                                                        >  2:190179/1‑141 (MQ=255)

aaTGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTa                                                                                                                                        >  1:908269/1‑141 (MQ=255)

aaTGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTa                                                                                                                                        <  1:336098/141‑1 (MQ=255)

 aTGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAg                                                                                                                                       >  1:381312/1‑141 (MQ=255)

  tGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAgg                                                                                                                                      >  2:113973/1‑141 (MQ=255)

    aTAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGt                                                                                                                                    <  2:190413/141‑1 (MQ=255)

       aaTGTAATAGGCTTGGAGCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAGTTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGtttt                                                                                                                                 <  1:719578/141‑1 (MQ=255)

          gTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTgag                                                                                                                              >  1:880499/1‑141 (MQ=255)

          gTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTgag                                                                                                                              >  3:230686/1‑141 (MQ=255)

          gTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTgag                                                                                                                              >  1:736285/1‑141 (MQ=255)

            aaTAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGAt                                                                                                                            <  4:275683/141‑1 (MQ=255)

                ggCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATacac                                                                                                                        <  1:797027/141‑1 (MQ=255)

                ggCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATacac                                                                                                                        >  3:22156/1‑141 (MQ=255)

                ggCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATacac                                                                                                                        <  2:791516/141‑1 (MQ=255)

                  cTTGAATCTGCATCTTGGATTGATCATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACtt                                                                                                                      >  1:606831/1‑141 (MQ=255)

                       aTCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGc                                                                                                                 <  3:398478/141‑1 (MQ=255)

                       aTCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGc                                                                                                                 <  1:4881/141‑1 (MQ=255)

                       aTCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGc                                                                                                                 <  1:5054/141‑1 (MQ=255)

                       aTCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTGTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATGAGGGTTTTGAGATACACTTTGTGc                                                                                                                 <  2:349499/141‑1 (MQ=255)

                        tCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCa                                                                                                                >  1:1018665/1‑141 (MQ=255)

                         cTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCaa                                                                                                               >  1:180673/1‑141 (MQ=255)

                            cATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGa                                                                                                            <  3:394447/141‑1 (MQ=255)

                             aTCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGAt                                                                                                           <  3:15229/141‑1 (MQ=255)

                              tCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATc                                                                                                          <  3:377550/141‑1 (MQ=255)

                               cTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCa                                                                                                         <  2:879020/141‑1 (MQ=255)

                                ttGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCAt                                                                                                        <  3:232514/141‑1 (MQ=255)

                                ttGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCAt                                                                                                        <  3:86927/141‑1 (MQ=255)

                                  ggATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATaa                                                                                                      <  3:309281/141‑1 (MQ=255)

                                       gATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCt                                                                                                 <  1:698744/141‑1 (MQ=255)

                                       gATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCt                                                                                                 <  2:121356/141‑1 (MQ=255)

                                         tAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTAc                                                                                               <  3:102303/141‑1 (MQ=255)

                                         tAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTAc                                                                                               <  1:163718/141‑1 (MQ=255)

                                         tAATACTGTATCCCAAGGATTCCAGTAAATAAGTGTTTCTTGGTCATTAATTTTAGCATTACCAACAGCTGCTAGCGCATGTCATAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTAc                                                                                               <  2:218798/141‑1 (MQ=255)

                                           aTTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTTTTTTTTTTTTATTTTATCATTACCAACAACTGTTAGTGCATGTCCTAATTGTTGATCATTTGTGTTTTGCGATACAATTTGTGAAAGAATTGTAATTCCtttgt                                                                                             >  4:367578/1‑137 (MQ=255)

                                           aTTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACAt                                                                                             <  2:908269/141‑1 (MQ=255)

                                            ttCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACAtt                                                                                            <  2:452355/141‑1 (MQ=255)

                                              cTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTAt                                                                                          >  2:650552/1‑141 (MQ=255)

                                                 tATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCtt                                                                                       <  2:1023058/141‑1 (MQ=255)

                                                 tATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCtt                                                                                       <  1:730089/141‑1 (MQ=255)

                                                  aTCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGGGCATGTCCTAAATGTGGATCATTAGGGTTTTGAGATACACTTTGTGCAAGAATCATAATTCCTACATTATCttt                                                                                      <  2:788744/141‑1 (MQ=255)

                                                   tCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCtttt                                                                                     <  1:584351/141‑1 (MQ=255)

                                                    cccAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTg                                                                                    <  2:185995/141‑1 (MQ=255)

                                                    cccAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTg                                                                                    <  1:891469/141‑1 (MQ=255)

                                                      cAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTa                                                                                  >  3:134642/1‑141 (MQ=255)

                                                        aGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAg                                                                                <  2:557270/141‑1 (MQ=255)

                                                         ggATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGt                                                                               <  2:29123/141‑1 (MQ=255)

                                                            ttCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGa                                                                            >  3:103249/1‑141 (MQ=255)

                                                              ccAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATc                                                                          <  1:356108/141‑1 (MQ=255)

                                                              ccAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATc                                                                          >  1:552258/1‑141 (MQ=255)

                                                              ccAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATc                                                                          <  4:21102/141‑1 (MQ=255)

                                                              ccAGTAAATAAGTTTTTCTTGGTCATTAATGTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGCTTTGAGATACACTTTGTGCAAGGATCATAATTGCTACATTATCTTTTGTAAGTGGATc                                                                          >  4:294103/1‑141 (MQ=255)

                                                               cAGTAAATAAGTTTTTCTAGGCCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTAGCTTTTGGAAGTTGATCa                                                                         >  2:237403/1‑141 (MQ=255)

                                                                    aaTAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTg                                                                    <  1:242356/141‑1 (MQ=255)

                                                                      tAAGTTTTTCTTGGTCATTAATTTTAGCATTACCTACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGtt                                                                  <  2:495905/141‑1 (MQ=255)

                                                                        aGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCa                                                                <  4:149791/141‑1 (MQ=255)

                                                                        aGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCa                                                                >  2:1059262/1‑141 (MQ=255)

                                                                        aGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGGTCa                                                                >  2:137268/1‑141 (MQ=255)

                                                                         gTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAACGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCat                                                               >  1:934865/1‑141 (MQ=255)

                                                                            tttCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGGGCATGTCCTAAATGTGGATCATTAGGGTTTTGAGATACACTTTGTGCAAGAATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATatg                                                            <  3:341970/141‑1 (MQ=255)

                                                                                ttGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATgg                                                        >  2:67917/1‑141 (MQ=255)

                                                                                 tGGTCATTAATTTTAGCATTACCAACAACTGCTAGGGCATGTCCTAAATGTGGATCATTAGGGTTTTGAGATACACTTTGTGCAAGAATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGt                                                       >  1:669741/1‑141 (MQ=255)

                                                                                      aTTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGTTACGcc                                                  >  2:486709/1‑141 (MQ=255)

                                                                                        tAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCctt                                                <  3:11475/141‑1 (MQ=255)

                                                                                         aaTTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCcttc                                               <  4:159952/141‑1 (MQ=255)

                                                                                         aaTTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCcttc                                               <  4:179230/141‑1 (MQ=255)

                                                                                           ttttAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTATTCATATGATGGTACGCattctt                                             >  2:267781/1‑141 (MQ=255)

                                                                                                gCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATaa                                        <  3:393935/141‑1 (MQ=255)

                                                                                                gCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATaa                                        >  1:948371/1‑141 (MQ=255)

                                                                                                    tACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGaa                                    <  4:303205/141‑1 (MQ=255)

                                                                                                    tACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGaa                                    <  1:49851/141‑1 (MQ=255)

                                                                                                       caacaaCTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAatat                                 <  2:930019/141‑1 (MQ=255)

                                                                                                       caacaaCTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAatat                                 <  2:975540/141‑1 (MQ=255)

                                                                                                        aacaacTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATAtc                                >  3:177555/1‑141 (MQ=255)

                                                                                                        aacaacTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATAtc                                >  2:347905/1‑141 (MQ=255)

                                                                                                          caacTGCTAGGGCATGTCCTAAATGTGGATCATTAGGGTTTTGAGATACACTTTGTGCAAGAATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATAtctc                              <  1:56712/141‑1 (MQ=255)

                                                                                                          caacTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATAtctc                              <  2:704942/141‑1 (MQ=255)

                                                                                                          caacTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATAtctc                              >  1:436523/1‑141 (MQ=255)

                                                                                                          caacTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATAtctc                              <  4:303272/141‑1 (MQ=255)

                                                                                                              tGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGcc                          >  2:877265/1‑141 (MQ=255)

                                                                                                              tGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTATTGATAATGAAGCTCTCTGcc                          <  4:204810/141‑1 (MQ=255)

                                                                                                               gttAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCt                         <  1:427709/139‑1 (MQ=255)

                                                                                                                cTAGGGCATGTCCTAAATGTGGATCATTAGGGTTTTGAGATACACTTTGTGCAAGAATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATTTGATGGTACGCCTTCTTGATAATGAATATCTCTGCCtt                        >  2:615041/1‑141 (MQ=255)

                                                                                                                      cATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGAtt                  <  3:205253/141‑1 (MQ=255)

                                                                                                                          tCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGAACATAATTCCTACATTCTCTTTTGTAAGTTGATCAACTTGTTCATATTATGGTACGCCTTCTTGATAATGAGTATCTCTGCCTTGTGATTTAcc              >  1:291867/1‑141 (MQ=255)

                                                                                                                            cTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGt            <  1:913635/141‑1 (MQ=255)

                                                                                                                              aaaTGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTAt          >  1:873581/1‑141 (MQ=255)

                                                                                                                               aaTGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTAtt         <  4:63421/141‑1 (MQ=255)

                                                                                                                               aaTGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTAtt         <  3:394696/141‑1 (MQ=255)

                                                                                                                                aTGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTc        <  2:166962/141‑1 (MQ=255)

                                                                                                                                   tgGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAAt     >  1:406383/1‑141 (MQ=255)

                                                                                                                                    gggTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGAGCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATc    <  2:1048166/141‑1 (MQ=255)

                                                                                                                                     ggTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATCa   <  3:275445/141‑1 (MQ=255)

                                                                                                                                     ggTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATCa   >  2:74086/1‑141 (MQ=255)

                                                                                                                                     ggTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATCa   >  2:588528/1‑141 (MQ=255)

                                                                                                                                     ggTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATCa   <  4:378756/141‑1 (MQ=255)

                                                                                                                                      gTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATCAt  <  4:185057/141‑1 (MQ=255)

                                                                                                                                      gTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATCAt  <  2:1053927/141‑1 (MQ=255)

                                                                                                                                      gTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATACCTCTGCCTTGTGATTTACCGTATTCAATCAt  <  1:630067/141‑1 (MQ=255)

AATGATAAATGTAATAGGCTTGAATCTGCATCTTGGATTGATAATTCTGTATCCCAAGGATTCCAGTAAATAAGTTTTTCTTGGTCATTAATTTTAGCATTACCAACAACTGCTAGCGCATGTCCTAAATGTGGGTCATTAGGGTTTTGAGATACACTTTGTGCAAGGATCATAATTCCTACATTATCTTTTGTAAGTTGATCAACTTGTTCATATGATGGTACGCCTTCTTGATAATGAATATCTCTGCCTTGTGATTTACCGTATTCAATCAT  >  USA300TCH1516_ALE/1052269‑1052543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`