| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,080,964 | G→A | 5.5% | L232L (TTG→TTA) | purM → | Phosphoribosylformylglycinamidine cyclo‑ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,080,964 | 0 | G | A | 5.5% | 454.4 / 5.4 | 141 | L232L (TTG→TTA) | purM | Phosphoribosylformylglycinamidine cyclo‑ligase |
| Reads supporting (aligned to +/- strand): ref base G (62/70); new base A (2/7); total (64/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 144 total aligned reads displayed.
ATTCATTCAAATGGATACAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATATTTG > USA300TCH1516_ALE/1080824‑1081097 | attcattcAAATGGATACAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTg > 1:1073887/1‑141 (MQ=255)attcattcAAATGGATACAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTg > 3:178525/1‑141 (MQ=255) caAATGGATACAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAaag > 3:140287/1‑141 (MQ=255) aaTGGATACAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAaagaa > 1:414057/1‑141 (MQ=255) ggATACAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGtt < 1:413249/141‑1 (MQ=255) gATACAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGttt > 2:290250/1‑141 (MQ=255) cAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTAt > 1:979672/1‑141 (MQ=255) cAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTAt > 1:810207/1‑141 (MQ=255) cAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTAt > 1:471347/1‑141 (MQ=255) cAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTAt < 1:130486/141‑1 (MQ=255) aGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTAtt > 2:346835/1‑141 (MQ=255) aGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAAATTGTACTTGCTTTGAAAAAAGAAGTTTATAtt > 2:194634/1‑141 (MQ=255) gTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTa > 1:719120/1‑141 (MQ=255) tAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAgg > 2:697502/1‑141 (MQ=255) tGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCaa < 1:923071/141‑1 (MQ=255) cgcAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATg > 2:459867/1‑141 (MQ=255) cAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGaa > 1:349119/1‑141 (MQ=255) cAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGaa < 2:307771/141‑1 (MQ=255) aaaataaatTAATGAATAAGGAATTAATTTGTCATCAAAATGCGATAATCGTCCATTTATCGATGTCTTATGAGAACTAACTAAATTATGTGTTATACCTGAAAAAAAAAAAAAAAAAGAAGTTTCTATTAAGGCAATGaa < 2:772198/140‑1 (MQ=255) ttaattaaTGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCat > 2:871422/1‑141 (MQ=255) taattaatGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCata < 2:183611/141‑1 (MQ=255) attaatGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATAtt < 3:27187/141‑1 (MQ=255) attaatGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATAtt < 3:204054/141‑1 (MQ=255) attaatGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATAtt < 2:392609/141‑1 (MQ=255) aGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAgg > 1:626573/1‑141 (MQ=255) aGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATAGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTAAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAgg > 3:90508/1‑141 (MQ=255) gCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGtt < 2:920917/141‑1 (MQ=255) gCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGtt < 1:931187/141‑1 (MQ=255) ttGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGttttt > 2:118410/1‑141 (MQ=255) gATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTAt < 1:195195/141‑1 (MQ=255) atattacATCAAACTGCGATAAACGTCCATTTATCTATGTCTTTTTAGAAACAACTAAATTATATCTCAAACCTGAAAAAAAAAAAAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATg < 4:410291/135‑1 (MQ=255) aTTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATg < 2:378367/141‑1 (MQ=255) tttGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGa > 2:427493/1‑141 (MQ=255) ttGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGaa < 1:468446/141‑1 (MQ=255) gCATCAAACTTCGATAATCGTCTATTTATCGATGTCTTTTTAGAACCATCTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAat < 4:316675/141‑1 (MQ=255) cATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGATGTTTTTATGAAAata < 3:301504/141‑1 (MQ=255) aTCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAatat < 3:18525/141‑1 (MQ=255) cAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTc < 4:408132/141‑1 (MQ=255) cAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTc > 2:113866/1‑141 (MQ=255) aCTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCAc < 1:871422/141‑1 (MQ=255) cTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACg < 2:1065752/141‑1 (MQ=255) gATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATAATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCa < 2:954510/141‑1 (MQ=255) aTAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCAt < 1:1023241/141‑1 (MQ=255) aTAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCAt < 2:158473/141‑1 (MQ=255) tAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCAtt > 3:104283/1‑141 (MQ=255) tAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCAtt > 1:721457/1‑141 (MQ=255) aaTCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCCTATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTg < 2:1074874/141‑1 (MQ=255) aaTCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTg > 1:1032344/1‑141 (MQ=255) aaTCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTg > 3:311981/1‑141 (MQ=255) tCGTCCATTTATAGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTAAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGcc < 2:475776/141‑1 (MQ=255) cGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCa > 3:197985/1‑141 (MQ=255) gTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAg > 2:150553/1‑141 (MQ=255) tCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGc < 2:766200/141‑1 (MQ=255) ccATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGcc < 2:243378/141‑1 (MQ=255) cATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCg < 2:1069744/141‑1 (MQ=255) tttATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGa < 1:775519/141‑1 (MQ=255) cGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATAtgc > 1:23109/1‑141 (MQ=255) cGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATAtgc > 1:656319/1‑141 (MQ=255) tGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATAtgctgc < 2:256023/141‑1 (MQ=255) tGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATAtgctgc < 2:559951/141‑1 (MQ=255) tCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTa < 1:83777/141‑1 (MQ=255) tCTTTTCAGAACCAACTAAATTATATGTCAAACCTGTACTTGATTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTa < 2:1039497/141‑1 (MQ=255) cTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTACCAGCCGGATATGCTGCTAg < 3:98588/141‑1 (MQ=255) ttttAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGaa > 3:393147/1‑141 (MQ=255) ttAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAAtt < 2:575872/141‑1 (MQ=255) ttAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAAtt < 1:302845/141‑1 (MQ=255) tAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTg > 2:980127/1‑141 (MQ=255) aGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGa > 1:30196/1‑141 (MQ=255) aaCCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGAta < 1:528977/141‑1 (MQ=255) ccAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGAtact > 1:192892/1‑141 (MQ=255) cTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTAcat < 1:429412/141‑1 (MQ=255) cTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTAcat < 4:375461/141‑1 (MQ=255) cTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTAcat < 1:833805/141‑1 (MQ=255) cTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCCAGAATTGATACTAcat < 2:645006/141‑1 (MQ=255) aaTTATATGTCAAACCTGTACTTGCTTTGAAAAAAGATGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATGCCACGTGCGTTGCCAGCCGGATATGCTGCGAGAATTGCTACTAcatcat < 4:239568/141‑1 (MQ=255) aaTTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTAcatcat > 4:242288/1‑141 (MQ=255) aTTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCAtt > 4:196068/1‑141 (MQ=255) ttATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCAttt < 3:105118/141‑1 (MQ=255) ttATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCAttt < 1:115657/141‑1 (MQ=255) atatGTCAAACCTGTACTTGCTTTAAAAAAAGAAGCTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTcc > 1:947628/1‑141 (MQ=255) atGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACtggtgg > 1:586681/1‑67 (MQ=255) tGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAac > 1:174701/1‑141 (MQ=255) tGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAac > 3:277890/1‑141 (MQ=255) gTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAaca < 1:578063/141‑1 (MQ=255) tCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAacac < 1:67101/141‑1 (MQ=255) tCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAacac > 2:899502/1‑141 (MQ=255) cAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACAcc < 1:350046/141‑1 (MQ=255) cAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACAcc < 4:219239/141‑1 (MQ=255) aaaCCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCa < 1:113866/141‑1 (MQ=255) aCCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCaaa < 1:1056904/141‑1 (MQ=255) aCCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTATTTATGCAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCaaa < 1:758531/141‑1 (MQ=255) cTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCaaaaa < 2:69872/141‑1 (MQ=255) tGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAat < 2:119340/141‑1 (MQ=255) tACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAatat > 2:117796/1‑141 (MQ=255) aCTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATAtt > 4:197637/1‑141 (MQ=255) aCTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGACATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATAtt > 1:908041/1‑141 (MQ=255) cTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATAttt > 1:1035044/1‑141 (MQ=255) cTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGAGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATAttt > 2:518742/1‑141 (MQ=255) ttGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATATTTg > 3:367925/1‑141 (MQ=255) ttGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATATTTg < 1:522440/141‑1 (MQ=255) | ATTCATTCAAATGGATACAGTTTAGTGCGCAAATTAATTAATGAATCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAACCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCAATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGATATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATATTTG > USA300TCH1516_ALE/1080824‑1081097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |