Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 81 N→G 100% intergenic (–/‑151)  / → repA –/replication protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000731810NG100.0% 151.2 / ‑6.5 48intergenic (–/‑151)–/repA–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (25/21);  minor base T (0/2);  total (25/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAG  >  CP000731/1‑219
                                                                                                                                           |                                                                                                                                          
gatatttatcaagggtttataaatccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGCTTGGTAATATAGTGTTTAGa                                                                                                                                           >  2:207080‑M1/60‑141 (MQ=255)
                 tataaacccttgctaaagctatattttcccttttatcagtttATTTAAAATGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTCATAGACGGGGTTTGGTACTCCAACGTATAGAacctgtttattttaatt                                                                                                                          <  2:90483‑M1/99‑6 (MQ=255)
                       cccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTCGAAACTGTTTATTTATATAATTTTc                                                                                                                    <  2:184437‑M1/105‑1 (MQ=255)
                         cttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGa                                                                                                                  >  1:47380‑M1/35‑141 (MQ=255)
                           tgataaagctatatttgacctgttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATa                                                                                                                >  1:147472‑M1/33‑141 (MQ=255)
                            gataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTATAAACTGTTTATTTCTATAATTTTCGATAc                                                                                                               <  2:45987‑M1/110‑1 (MQ=255)
                            gataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATAc                                                                                                               >  1:74893‑M1/32‑141 (MQ=255)
                             ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                              >  1:123960‑M1/31‑141 (MQ=255)
                             ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                              <  1:151589‑M1/111‑1 (MQ=255)
                                aagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGt                                                                                                           >  1:148683‑M1/28‑141 (MQ=255)
                                  gctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtat                                                                                                         <  1:130831‑M1/116‑1 (MQ=255)
                                         ttgcccgtgtataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATaa                                                                                                  >  1:18750‑M1/19‑141 (MQ=255)
                                          ttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAAt                                                                                                 <  1:191902‑M1/124‑1 (MQ=255)
                                          ttccattttataagtttATTTAAAGTGCTATAATAAATTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAAt                                                                                                 >  2:157412‑M1/18‑141 (MQ=255)
                                               ttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaata                                                                                            <  1:86305‑M1/129‑1 (MQ=255)
                                                   ataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGACTTCGTAATAAAACGCTTATAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAatat                                                                                        <  2:40604‑M1/133‑1 (MQ=255)
                                                     aagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCt                                                                                      >  1:124786‑M1/7‑141 (MQ=255)
                                                        tttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAg                                                                                   >  1:184321‑M1/4‑141 (MQ=255)
                                                        tttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAg                                                                                   >  1:78599‑M1/4‑141 (MQ=255)
                                                          tATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTg                                                                                 >  1:42507‑M1/2‑141 (MQ=255)
                                                           aTTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGc                                                                                <  1:51717/141‑1 (MQ=255)
                                                             ttAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCaa                                                                              <  1:61339/141‑1 (MQ=255)
                                                              tAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAg                                                                             <  2:52496/141‑1 (MQ=255)
                                                                   tGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCt                                                                        <  1:47749/141‑1 (MQ=255)
                                                                    gCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCTGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTa                                                                       <  2:24779/141‑1 (MQ=255)
                                                                     cTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGACTAATATCTTAGTGCAAGCACCTaa                                                                      >  1:35532/1‑141 (MQ=255)
                                                                     cTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTaa                                                                      >  2:114185/1‑141 (MQ=255)
                                                                         aataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCa                                                                  <  1:59954/141‑1 (MQ=255)
                                                                          ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                                 <  2:168155/141‑1 (MQ=255)
                                                                            aaaCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAtaa                                                               >  2:78438/1‑139 (MQ=255)
                                                                            aaaCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATta                                                               >  1:63598/1‑141 (MQ=255)
                                                                               cTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATACCTTGATGAATATCTTAGTGCAAGCACCTAAATCATtaaat                                                            >  1:43786/1‑141 (MQ=255)
                                                                                ttAATATAACAAAAACCCCAACTACTTGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATCATATCCTAGTGCAAGCACCTAAATCATtaaata                                                           <  2:132009/141‑1 (MQ=255)
                                                                                  aaTATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataaa                                                         >  1:197673/1‑141 (MQ=255)
                                                                                   atatAACAAAAACCCCAACTACTGGTAAAAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTaaataaat                                                        >  2:230919/1‑141 (MQ=255)
                                                                                             aaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaa                                              >  2:224977/1‑141 (MQ=255)
                                                                                                ccacactactGGTATTTGTCGGGGTTTTCTAATAAAACGTTTAGAAATTGTTTATTTATATAATTTTCGATACTTGTATTATATAAAGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                           <  2:230066/137‑1 (MQ=255)
                                                                                                 ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                          >  1:122271/1‑141 (MQ=255)
                                                                                                  cAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataaa                                         <  1:181151/141‑1 (MQ=255)
                                                                                                     ctactGGTAATAGTCAGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAAc                                      <  2:111279/141‑1 (MQ=255)
                                                                                                            tAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTttat                               <  2:164993/141‑1 (MQ=255)
                                                                                                              attgTCGTGTTTTGGGAATAGAACGTTTAGATACATTTTATTTATATAATTTTCGATGCTTGTATTATATAATGAATAATATCGTAGTGCAAGCACCTAAATCATTAAATAAATGGAAAGCTAAATAAAAACGTTttatta                             <  2:163929/138‑1 (MQ=255)
                                                                                                                      ggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAc                     >  1:214343/1‑141 (MQ=255)
                                                                                                                        tttGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCa                   <  1:58137/141‑1 (MQ=255)
                                                                                                                                     cGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATgg      >  2:223858/1‑141 (MQ=255)
                                                                                                                                     cGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATgg      >  1:192139/1‑141 (MQ=255)
                                                                                                                                       tttAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAAATTAAAGCTAAATAAAAACTTTTTATTAACACTTACCAAACTCAAATATGGaa    >  2:58381/1‑141 (MQ=255)
                                                                                                                                         tAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAag  <  2:149381/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAG  >  CP000731/1‑219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: