BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	100%	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	100.0%	58.2 / NA	21	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (9/12); total (9/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATAT  >  USA300TCH1516_ALE/1646056‑1646322

aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                >  1:81698/1‑141 (MQ=255)

   aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                             <  1:47588/141‑1 (MQ=255)

          acacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGGCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                      >  1:120698/1‑141 (MQ=255)

                  tgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTc                                                                                                              >  2:62786/1‑141 (MQ=255)

                    gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                            <  1:23933/141‑1 (MQ=255)

                                           gCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTATAATATTGCCTTTAATTCTTTTTAAGTTATTCCTCTTTTTACAACAATTAAATTATATTCATATTTTTCATCAACAAAATTAgct                                                                                     >  2:195319/1‑141 (MQ=255)

                                            cTACAATCGGATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                                                                    <  2:47244/141‑1 (MQ=255)

                                                 aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                               <  2:733/141‑1 (MQ=255)

                                                 aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                               <  1:95652/141‑1 (MQ=255)

                                                       tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                         >  1:210597/1‑141 (MQ=255)

                                                             gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCa                                                                   <  1:224959/141‑1 (MQ=255)

                                                              atgGTCCATCTGCTTCTAATTCTAGCATTTTGCTTTCTATTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACTGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCaa                                                                  <  2:42304/141‑1 (MQ=255)

                                                               tgGTCCATCTCCTCCTTATTCTAGCATTCTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAt                                                                 <  1:63803/141‑1 (MQ=255)

                                                                     agtcgCTCCTAATTCTAGCATTTGGCTTTCTAATATTGCCTTTAATTCTTTTGCAGTCATGCCTCCTTTTACAACAGTTAGAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                           <  1:165399/137‑1 (MQ=255)

                                                                                 ttCTAGCATTTTGCTTTCTAATATTGCCTTTAGTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATACATTCATATGTTTCATCAACAATATTATCTTCTTTTTGAAGTAAAGCAATTTCGTCAGCATCTTTGAc                                               >  2:208522/1‑141 (MQ=255)

                                                                                  tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATCTGTTTCATCAACAATATTACCTGCTTTTTGAATTAAATCAATTTCGTAAGCATCTTTGAct                                              >  2:42616/1‑140 (MQ=255)

                                                                                                                  ttCTATTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt              <  2:81698/141‑1 (MQ=255)

                                                                                                                      ttttCAGTCATGCATCATTTTAAAAGAGTTGAAATATATTCATATGTTTCATCAACACTATTAGCTGCGTGTTGAATTAAATGAATTTCGTCAGCATCTGTGACGTCTCTAATTTTATCTACAGTATTAGAACTGCttatt          <  2:39322/141‑1 (MQ=255)

                                                                                                                         tCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAAt       <  2:163658/141‑1 (MQ=255)

                                                                                                                              cATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGTATTAAAGCAATTTCCTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTCTTAGAACTGCTTATTACTGatat  >  1:12901/1‑141 (MQ=255)

                                                                                                                              cATGCCTCCTTTTACAACAGTTAAAATATAAGCATATGTTTCATCAACAATATTAGCTGCTTTTTTAATTAAAGCAATTTCGTCAGAATCTTTGACGTCTATAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat  >  2:108435/1‑141 (MQ=255)

ATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATAT  >  USA300TCH1516_ALE/1646056‑1646322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`