Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,744,292 A→T I127N (ATT→AAT)  apt ← Adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,744,2920AT100.0% 80.0 / NA 25I127N (ATT→AAT) aptAdenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/13);  total (12/13)

ATTCGTCGTATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATT  >  USA300TCH1516_ALE/1744155‑1744427
                                                                                                                                         |                                                                                                                                       
attCGTCGTATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCg                                                                                                                                      <  1:68662/141‑1 (MQ=255)
   cgtcgtATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTCATTTTTCACCTAATTTTATTGCTGCTTCATTCGTac                                                                                                                                   >  2:253557/1‑141 (MQ=255)
       gtATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTaccacc                                                                                                                               <  1:71300/141‑1 (MQ=255)
       gtATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTaccacc                                                                                                                               <  1:286508/141‑1 (MQ=255)
       gtATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTaccacc                                                                                                                               <  1:299551/141‑1 (MQ=255)
               ttAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCta                                                                                                                       <  2:266172/141‑1 (MQ=255)
                tAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCtaa                                                                                                                      <  1:7037/141‑1 (MQ=255)
                                                  aTACCATTTAAATATTTCAATTAAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGt                                                                                    >  1:71160/1‑141 (MQ=255)
                                                     ccATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTACTTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTa                                                                                 <  1:82374/141‑1 (MQ=255)
                                                         ttAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCGCCTGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTg                                                                             <  2:224920/141‑1 (MQ=255)
                                                          tAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGc                                                                            >  2:84015/1‑141 (MQ=255)
                                                          tAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATCCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTTGTTTAATCGc                                                                            >  2:120288/1‑141 (MQ=255)
                                                                 ttcaattcaaTTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATATATACTTAACACACGTTGACCTGGTTTAATTGCATCTTtg                                                                     >  1:240241/1‑141 (MQ=255)
                                                                 ttcaattcaaTTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAAAAAATAATCTGTAATTAAAACACCATGACCTGGTTTAATTGCATCtttt                                                                     >  1:240232/1‑140 (MQ=255)
                                                                     attcaattATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCa                                                                 >  1:301008/1‑141 (MQ=255)
                                                                                       tACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAc                                               <  2:274392/141‑1 (MQ=255)
                                                                                        aCCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAcc                                              >  2:9145/1‑141 (MQ=255)
                                                                                        aCCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAcc                                              <  1:187546/141‑1 (MQ=255)
                                                                                        aCCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAcc                                              >  1:277272/1‑141 (MQ=255)
                                                                                         ccTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCa                                             >  1:215420/1‑141 (MQ=255)
                                                                                              tacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTc                                        <  2:139937/141‑1 (MQ=255)
                                                                                                                  cAACTTATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGaa                    >  2:58965/1‑141 (MQ=255)
                                                                                                                  cAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGaa                    >  2:141057/1‑141 (MQ=255)
                                                                                                                               tgctgcTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg       <  2:301008/141‑1 (MQ=255)
                                                                                                                                    ctTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAtt  <  2:71160/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATTCGTCGTATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATT  >  USA300TCH1516_ALE/1744155‑1744427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: