Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073125,6630A.94.5% 371.8 / 11.1 91coding (316/333 nt)USA300HOU_pUSA300HOUMR0031hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base . (45/41);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAA  >  CP000731/25523‑25798
                                                                                                                                            |                                                                                                                                       
attgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATa                                                                                                                                         >  1:323128/1‑141 (MQ=255)
attgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATAAAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATa                                                                                                                                         >  1:359271/1‑141 (MQ=255)
 ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATATTACAAATaa                                                                                                                                        >  2:187483/1‑141 (MQ=255)
 ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTCTAGCACTATTTATTATGCTGTTTAATTACTCTACTACAAATaa                                                                                                                                        >  2:146577/1‑141 (MQ=255)
   ctatGTTATTTACAGTAGTGTTTAAATTTACAGTTTTGTCACCAAGTATTGAAGGATCTTGGATTTATCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTACTTCCTCTACTACAAATaaaa                                                                                                                                      <  2:298819/140‑1 (MQ=255)
        ttATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatat                                                                                                                                 <  2:350784/141‑4 (MQ=255)
        ttATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTATAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatat                                                                                                                                 >  1:174497/1‑138 (MQ=255)
         tATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatata                                                                                                                                <  1:313943/141‑5 (MQ=255)
           tttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACa                                                                                                                             <  1:179734/141‑1 (MQ=255)
           tttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACa                                                                                                                             >  1:371463/1‑141 (MQ=255)
            ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCACTATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACAt                                                                                                                            >  1:247576/1‑141 (MQ=255)
            ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTCTTTCTTATGCTGTTTAATTACTATACTACAAATAAAACATATACAt                                                                                                                            >  2:335594/1‑141 (MQ=255)
            ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTCTGCTGTTTAATTACTATACTACAAATAAAAAATATACan                                                                                                                            >  1:253304/1‑140 (MQ=255)
            ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACAt                                                                                                                            >  2:203778/1‑141 (MQ=255)
              acacTAGAGCTTAAATTTACATGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCt                                                                                                                          <  2:238862/141‑1 (MQ=255)
               cacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTa                                                                                                                         <  1:410782/141‑1 (MQ=255)
                acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaa                                                                                                                        >  2:361196/1‑141 (MQ=255)
                acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaa                                                                                                                        >  2:32381/1‑141 (MQ=255)
                aatAGTGATTAATGTTACAATTTTGTTAGCAAGTAATAAAATATCTTAGTTTTCTCAATATCTATAAATTTTCTTTATAATTTTAACACTATTTCTTATGCTGTTTAATTACAATACTACAAATAAAAAATATACATCTaa                                                                                                                        <  2:233318/139‑1 (MQ=255)
                    tttcTTAAATTTACAGATTTGTCACCAAATATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGt                                                                                                                    <  1:207196/138‑1 (MQ=255)
                    atGCTTAGATTTACAGGTTTGGCACCAAGTATTGAAGGATCTTGGTGTTCTCAATATCCAGAAATTTTCTTTATAATTGTAACACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGt                                                                                                                    <  2:313126/140‑1 (MQ=255)
                      gCTTAAATTGACAGGTTTGTCACCAAGTATTGAAGGATCTTTGTTTTCTCAATAGCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTa                                                                                                                  <  2:238710/141‑1 (MQ=255)
                       tttAAATTTAAAGGTTTGTCTCCAAGGATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTATAAATAAAAAATATACATCTAAAGGTTaa                                                                                                                 <  2:258098/140‑1 (MQ=255)
                        ttAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTACTCATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGTTTaaa                                                                                                                >  2:323069/1‑141 (MQ=255)
                        ttAAATTTACAGGTCTGTAAACAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTaaa                                                                                                                <  2:331/141‑1 (MQ=255)
                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAAt                                                                                                               >  2:94460/1‑141 (MQ=255)
                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAAt                                                                                                               >  1:214333/1‑141 (MQ=255)
                           aaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATTCTACAAATAAAAAATATACATCTAAAGGTTAACTTa                                                                                                             >  2:140152/1‑141 (MQ=255)
                           aaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTGTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGATAAATTa                                                                                                             >  2:7248/1‑141 (MQ=255)
                            aTTTACAGGTTTGTAACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTAGTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTaa                                                                                                            <  1:204509/141‑1 (MQ=255)
                               tACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTTAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGa                                                                                                         <  2:229049/141‑1 (MQ=255)
                               tACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGa                                                                                                         <  2:191300/141‑1 (MQ=255)
                               tACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGa                                                                                                         >  1:112281/1‑141 (MQ=255)
                                 cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGTTTAATTTAATGaaa                                                                                                       >  2:37584/1‑141 (MQ=255)
                                    gTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAAt                                                                                                    <  1:276702/141‑1 (MQ=255)
                                    gTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAAt                                                                                                    <  2:109273/141‑1 (MQ=255)
                                       tGTCATCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGa                                                                                                 <  2:276063/141‑1 (MQ=255)
                                        gTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTAAAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGaa                                                                                                <  2:126878/141‑1 (MQ=255)
                                           aCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTa                                                                                             >  1:141474/1‑141 (MQ=255)
                                               aGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATAAATGAAAAATAGAATTAgaga                                                                                         >  2:236276/1‑141 (MQ=255)
                                                    tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTGGCACTATTAGTTATGCTGTTTAATTACTATACTACAAATAAATAGTATACATCTAATGGTTTAATTACTGAAAAATAGAATCATAGAataaa                                                                                    >  2:53156/1‑139 (MQ=255)
                                                    tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGTTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                    >  2:301849/1‑141 (MQ=255)
                                                    tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTACAGAAtata                                                                                    >  2:146979/1‑141 (MQ=255)
                                                    tGAAGGATCTTGGTTTTAACCATGTCCAGAAATTTTCTTTATAATTGTGGCACTACGCCTTATGTCGGCGATTTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATCAAAAATAGAATTAGAGAAtata                                                                                    >  2:92790/1‑141 (MQ=255)
                                                    tGAAAGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCAATATTTATTATGCTGTTTAATTACTATAATACAAATAAAAAATATACATCTAAATGTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                    <  2:265330/141‑1 (MQ=255)
                                                      aaGGATCTTGGTTTTATCAATATCCAGAAAGTTTCTTTATAATTGTAGAACTATTTATTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                  <  2:203951/141‑1 (MQ=255)
                                                       aGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGaa                                                                                 <  1:397134/141‑1 (MQ=255)
                                                           tCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACa                                                                             >  2:248059/1‑141 (MQ=255)
                                                           tCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACa                                                                             <  1:106844/141‑1 (MQ=255)
                                                            cTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaa                                                                            >  2:108869/1‑141 (MQ=255)
                                                              tGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaa                                                                          <  2:180317/141‑1 (MQ=255)
                                                               ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACGAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGACTATAGAAAAcaacaa                                                                         >  2:378989/1‑141 (MQ=255)
                                                               ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                         >  1:243159/1‑141 (MQ=255)
                                                               ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                         >  2:79265/1‑141 (MQ=255)
                                                                 ttttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAgg                                                                       >  1:111158/1‑141 (MQ=255)
                                                                 ttttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACGACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAga                                                                       >  1:419118/1‑140 (MQ=255)
                                                                  tttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAACTATTACTTCGACAACCAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                      >  1:202875/1‑141 (MQ=255)
                                                                   ttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGaa                                                                     >  2:274569/1‑141 (MQ=255)
                                                                      tcAATATCCAGAAATTTTCTTTATACTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGTTTAAATTAATAAAAAATAGAATTAGAGAATATAGAAAACAAAAAGAAAttt                                                                  >  2:37359/1‑141 (MQ=255)
                                                                       aaaTATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtc                                                                 <  1:129059/140‑1 (MQ=255)
                                                                          tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCa                                                              >  2:70046/1‑141 (MQ=255)
                                                                          tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCa                                                              >  1:326056/1‑141 (MQ=255)
                                                                           atCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCaa                                                             <  1:134968/141‑1 (MQ=255)
                                                                           atCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCaa                                                             <  1:364408/141‑1 (MQ=255)
                                                                            tCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                            <  2:323128/141‑1 (MQ=255)
                                                                             ccAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGa                                                           <  1:215781/141‑1 (MQ=255)
                                                                             acAGAAATTTTCATTATAATTGTAGCACTATATATTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGa                                                           <  2:25837/140‑1 (MQ=255)
                                                                                 aaaTTTTCTTTATAATTGTAGAACTATTTCTTATGCTGTTTAAATACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACt                                                       <  2:202356/141‑1 (MQ=255)
                                                                                       tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAAATCCCTCTAAATTTTAATTTCAGGTTGAATATTATTAGAGAATATAGAAAACAACAAGGAATTTCTCAAGAAACTTTAAGt                                                 >  1:406736/1‑141 (MQ=255)
                                                                                       tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGTTTTCATGTACATAGAAAACAAAGAGGAATTTCTCAAGAAACTTTAAGt                                                 >  1:183824/1‑141 (MQ=255)
                                                                                        cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTa                                                >  2:402023/1‑141 (MQ=255)
                                                                                        cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTa                                                >  1:280365/1‑141 (MQ=255)
                                                                                        cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTa                                                >  1:41811/1‑141 (MQ=255)
                                                                                            ataATTGTAGGACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGa                                            <  2:28899/141‑1 (MQ=255)
                                                                                              aactGTAGTACGATTTCTTATGCTGTTTAATTACTATAATAAAAATAAAAAATATAAATCTAAAGGTTAAATTAATGAAAAATAGAATTAGATAATATATAAAACAAAAAGTAATTTCTCAAGAAACTTTAAGTAAAGAAt                                          <  2:253304/138‑1 (MQ=255)
                                                                                              aaTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAt                                          <  1:399355/141‑1 (MQ=255)
                                                                                               aTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAATGTTAAATTAATGAAAAATAGAATTAGAGAATATCGAAAACAAATTGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                         <  1:83358/141‑1 (MQ=255)
                                                                                               aTTGTAGAAATATTTATTATGATGTTTAATAACAATACAAAAAAAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                         <  2:322808/141‑1 (MQ=255)
                                                                                                   tAGCACTATTTCTTATGCTGTTTAATTACTATACGACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaaa                                     >  1:274725/1‑141 (MQ=255)
                                                                                                     gCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgt                                   >  2:137758/1‑141 (MQ=255)
                                                                                                     gCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgt                                   >  2:229674/1‑141 (MQ=255)
                                                                                                      cACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtg                                  >  2:38423/1‑141 (MQ=255)
                                                                                                       aatatttattATGATGTATAATAACTATAAAAAAAAAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtgt                                 <  2:330082/139‑1 (MQ=255)
                                                                                                        aaaatttttATGGTGAATAATTAATAAAATAAAAATAAAAAATATACATCTAAAAGTTAAATTAATGAAAAATAGAATTAGAGAAAAAAAAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTc                                <  2:100563/134‑1 (MQ=255)
                                                                                                         tATTACTTATGCTGTGTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAAAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCt                               <  2:429808/141‑1 (MQ=255)
                                                                                                           tttATTATGATGTATAATTACTAAAATACAAATAAAAAATATACATATAAAGGTTAAATTAATGAAAAATAGAATTAGAAAATAAAAAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                             <  2:12033/141‑1 (MQ=255)
                                                                                                            ttCTTATGCTGTTTAATTACTATACTACAAATAAAACATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAAAATTAAAAGTGTATAgc                            >  2:316831/1‑140 (MQ=255)
                                                                                                            ttCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAgg                            <  2:297950/141‑1 (MQ=255)
                                                                                                            ttCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAAGAGAATTAGAGCCATGGGTATAGAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAgg                            >  1:185564/1‑141 (MQ=255)
                                                                                                             taatATGATGTTGAATTACTATACAAAAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGc                           <  2:409503/138‑1 (MQ=255)
                                                                                                             tCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGc                           >  2:239919/1‑141 (MQ=255)
                                                                                                                   gCTGTTTAATTACTAAACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTa                     <  1:48642/141‑1 (MQ=255)
                                                                                                                          aaTTAATAAACGACAAAAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGc              <  2:17947/141‑1 (MQ=255)
                                                                                                                              actctactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATa          <  1:375868/141‑1 (MQ=255)
                                                                                                                              actatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATa          <  1:138575/141‑1 (MQ=255)
                                                                                                                              acgatactaCAAATAAAAAAAAGACACATAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATAAAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATa          <  2:33484/138‑1 (MQ=255)
                                                                                                                                tatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGa        >  1:378695/1‑141 (MQ=255)
                                                                                                                                     tacAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGTCAAACTATTAATGCAATAGAAAACa   >  2:281043/1‑141 (MQ=255)
                                                                                                                                      acAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaa  <  1:276392/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                       
ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAA  >  CP000731/25523‑25798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: