| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 493,809 | G→T | 11.4% | V82L (GTA→TTA) | bltD_1 → | Spermine/spermidine acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 493,809 | 0 | G | T | 11.4% | 86.6 / 3.1 | 35 | V82L (GTA→TTA) | bltD_1 | Spermine/spermidine acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (13/18); new base T (2/2); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01 | |||||||||||
CGCCAGAGGAAAATATTACAGCTGCAATGTCTGATAATGAAAGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTAACGGTTAATACTGACAAT > USA300TCH1516_ALE/493669‑493949 | ggCCATATGAAGATGTGACAGGTGCAATGTATGATAATGAAAGGTAGCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTGTTACATTACATCGGTGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAg < 2:196963/140‑1 (MQ=255)cGCCAGAGGAACATATTACAGCTGCAATGTCTGATAATGAATGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAg < 2:309830/141‑1 (MQ=255) gCCAGAGGAAAATATTACAGCTGCAATGTATGATAATGAAAGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGt < 2:173619/141‑1 (MQ=255) gagGAAAATATTACAGCTGCAATGTCTGATGATGAAAGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGCTGCAGTAttt > 1:207113/1‑141 (MQ=255) agGAAAATATTACAGCTGCAATGTCTGATAATGAAAGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTAtttt < 2:336274/141‑1 (MQ=255) aaTATTACAGCTGCAATGTCTGATAATGAAAGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAgg < 1:93390/141‑1 (MQ=255) gCTGCAATGTCTGATAATGAAAGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGAGGGGTCGCCATTTAGCGATAACCAAGATGTAGTATTTTTAGGGTCATTTAga > 2:293641/1‑140 (MQ=255) aTGTCTGATAATGAAAGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTCCCGGTCATTTAGTGTTGAt > 2:164679/1‑141 (MQ=255) gAAAGGTATCCCATCGTGGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGt < 2:67453/141‑1 (MQ=255) aaGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCCTTTAGCGATAACCAAAATGCCGTATTTTTCCGGTCATTTAGCGTTGATCAACGTTATCGTaa > 1:38046/1‑141 (MQ=255) aaGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTaa < 1:34885/141‑1 (MQ=255) ggTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTTTTTTTTTTCGTGGAAAAGAGGTCGCACCATTTAGCGATATCGAAGCTGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGtctta > 2:26359/1‑137 (MQ=255) tgccgttCGTTGTAATGGATGGTAGGCAATGTGTGTCCTGTTTTAGATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGTTTCTTAGGTATTTATCTACGTTATCGTAATagag < 2:135557/135‑1 (MQ=255) cATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAAt < 1:217732/141‑1 (MQ=255) cGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGCTACCGTACTAGAGGCATAgg > 1:58510/1‑141 (MQ=255) gTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTaaa < 1:419103/141‑1 (MQ=255) tggCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCAACATTTAGCGATAACCAAGATGCAGTTTTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAAt < 1:336041/141‑1 (MQ=255) gcaggcaATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATgg > 1:393883/1‑141 (MQ=255) caggcaATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGGATCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGa < 2:234871/141‑1 (MQ=255) caATGTGTGGCGTTTTTTACATTACATCGGGGAAAAGGGGTCGTACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGaaaaa < 1:393267/141‑1 (MQ=255) tgGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCATTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGt > 1:123209/1‑141 (MQ=255) tgGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCATTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGt > 1:414269/1‑141 (MQ=255) tttttACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTACAGTGGTAATGGAAAAATTGGCGTCATTTa > 2:360042/1‑141 (MQ=255) tttACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATc < 1:86230/141‑1 (MQ=255) aattacgtAGTGGAAAATGTGTCGCAACATTTAGTGATAACGAAGAAGTAGTATTTTTCAGGTTATTTAGTGTTGATCAAAGTTATCGTAATAGTGGAATTGGTAAAGTGGTAATGGAAAAATTGTCATCATTTAACACtt < 2:266879/140‑1 (MQ=255) attacatCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTc < 2:294774/141‑1 (MQ=255) ttacatCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGGGGCATAGGTACAGTGGTAATGGAACAATTGGCGTCATTTATCACTTCa > 1:148514/1‑141 (MQ=255) ggggggAAGAGGGGGCGGAACATGTAGCGAGAACCAAGGGGCAGTATTGTTCAGGTGATTTAGGGCTGATCAAGGTTAGCGTAATAGAGGAAGAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACAtt < 2:127391/137‑1 (MQ=255) aaaaGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTAAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATCGGTAAAGTGGTACTGGAAAAATTGGAGTCATACATCACCTCAACATTTCAAGa > 2:267621/1‑141 (MQ=255) aaaaGGGGTCGCACCATGTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGa < 1:39591/141‑1 (MQ=255) cATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATtg < 1:360042/141‑1 (MQ=255) aGCGATAACCAAGATGCAGTATTTGTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTa > 1:63301/1‑141 (MQ=255) gCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTaa > 1:355974/1‑141 (MQ=255) cGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTAAc < 1:192264/141‑1 (MQ=255) aCCAAGATGCATTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTAACGGTTa < 2:414269/141‑1 (MQ=255) aCCAAGATGCATTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTAACGGTTa < 2:123209/141‑1 (MQ=255) gATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTAACGGTTAATACt > 1:426742/1‑141 (MQ=255) gTATTTTTCAGGTCATTTAGTGTTGATGAATGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTAACGGTTAATACTGACAAt > 2:375053/1‑141 (MQ=255) | CGCCAGAGGAAAATATTACAGCTGCAATGTCTGATAATGAAAGGTATCCCATCGTTGTAATGGATGGCAGGCAATGTGTGGCCTTTTTTACATTACATCGTGGAAAAGGGGTCGCACCATTTAGCGATAACCAAGATGCAGTATTTTTCAGGTCATTTAGTGTTGATCAACGTTATCGTAATAGAGGAATAGGTAAAGTGGTAATGGAAAAATTGGCGTCATTTATCACTTCAACATTTCAGGATATTAATGAGATTGTGTTAACGGTTAATACTGACAAT > USA300TCH1516_ALE/493669‑493949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |