Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000731 25,669 (A)7→6 coding (322/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073125,6630A.97.7% 386.0 / 1.6 85coding (316/333 nt)USA300HOU_pUSA300HOUMR0031hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base . (43/40);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAAT  >  CP000731/25523‑25799
                                                                                                                                            |                                                                                                                                        
attgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATa                                                                                                                                          <  1:67369/141‑1 (MQ=255)
   gtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaa                                                                                                                                       >  1:268873/1‑141 (MQ=255)
   gtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaa                                                                                                                                       >  2:273013/1‑141 (MQ=255)
    tatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaa                                                                                                                                      >  2:52414/1‑141 (MQ=255)
      tGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaat                                                                                                                                    >  1:239190/1‑140 (MQ=255)
      tGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaaa                                                                                                                                    >  1:174744/1‑141 (MQ=255)
        ttATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatat                                                                                                                                  >  1:286099/1‑138 (MQ=255)
          aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATAc                                                                                                                               >  2:168785/1‑141 (MQ=255)
             tACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATc                                                                                                                            >  1:224531/1‑141 (MQ=255)
             tACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATc                                                                                                                            >  1:344709/1‑141 (MQ=255)
             tACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATc                                                                                                                            <  1:351227/141‑1 (MQ=255)
              acacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCt                                                                                                                           <  1:359654/141‑1 (MQ=255)
                       cTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTaa                                                                                                                  >  2:123106/1‑141 (MQ=255)
                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTGCTATACTACAAATAGAAAATATACATCTAAAGGTTaatt                                                                                                                >  1:15357/1‑139 (MQ=255)
                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAAt                                                                                                                >  1:38591/1‑141 (MQ=255)
                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAAt                                                                                                                >  1:241820/1‑141 (MQ=255)
                          aaaTTTACAGGTTTTTCACCAAGTATTTAAATATCTTGTTTTTCTCAATATCCAGAAATTGTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAAtt                                                                                                               <  1:123397/141‑1 (MQ=255)
                          aaaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAAtt                                                                                                               <  1:146635/141‑1 (MQ=255)
                           aaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATACAGAAAATTTATTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTaaaaaaa                                                                                                              <  2:160384/141‑4 (MQ=255)
                            aTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTaa                                                                                                             <  2:15961/141‑1 (MQ=255)
                            aTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTaa                                                                                                             <  2:17174/141‑1 (MQ=255)
                              ttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATg                                                                                                           <  2:112461/141‑1 (MQ=255)
                               tACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGa                                                                                                          <  2:355276/141‑1 (MQ=255)
                               tACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGa                                                                                                          <  2:258116/141‑1 (MQ=255)
                                aCAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTGTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaa                                                                                                         >  1:131009/1‑141 (MQ=255)
                                 cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                        >  1:281075/1‑141 (MQ=255)
                                 cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                        >  2:174080/1‑141 (MQ=255)
                                 cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                        >  1:204887/1‑141 (MQ=255)
                                 cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                        >  2:346082/1‑141 (MQ=255)
                                  aGGTTTTTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTCTTTATTATGATGTTTAATTAATACAATACAAATAACAAATCTACATCTAAAGGTTAAATTAATGaaaa                                                                                                       <  1:268638/141‑1 (MQ=255)
                                     tttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATa                                                                                                    >  1:381991/1‑141 (MQ=255)
                                      ttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAGATTAATGAAAAATAg                                                                                                   >  1:344755/1‑141 (MQ=255)
                                           aCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTa                                                                                              >  1:334790/1‑141 (MQ=255)
                                           aCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTa                                                                                              >  2:317770/1‑141 (MQ=255)
                                           aCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTa                                                                                              >  2:257648/1‑141 (MQ=255)
                                             cAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTaga                                                                                            >  2:316809/1‑141 (MQ=255)
                                                     gAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAg                                                                                    >  2:838/1‑141 (MQ=255)
                                                       aGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTTTTTAATTACTTTGCGACGAATATTAAATATACATTGAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGaa                                                                                  >  1:144028/1‑141 (MQ=255)
                                                          aTCTTGGTTTTCTAAATATCCAGAAATTTTCTTTATAATTGAAGCAATATTTCTTATGCTGTTTAATTATTAAAAAAAAAAAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAAc                                                                               <  2:173994/141‑1 (MQ=255)
                                                            cTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaa                                                                             >  2:152873/1‑141 (MQ=255)
                                                             ttGGTTTTATCAATATCCAGAAATTTTCTTTATAATTGTAGCAATATTTCTTATGATATTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaa                                                                            >  2:246020/1‑141 (MQ=255)
                                                              tGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTAAAAAAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaa                                                                           <  2:101988/141‑1 (MQ=255)
                                                              tGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCAATATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATAaaaaaaaaaaaa                                                                           <  2:225229/141‑1 (MQ=255)
                                                              tGGTTTTATCAATATCCAGAAATTTTCTTTATAATTGTAGCAATATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaa                                                                           <  2:3235/141‑1 (MQ=255)
                                                               ggTTTTCTCAATATCCNGAAATTTTCTTTNTAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                          >  1:265012/1‑141 (MQ=255)
                                                               ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATCAAAACTATCCATCTAAAGGTTACATTAATGACAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                          <  1:164778/141‑1 (MQ=255)
                                                               ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                          >  1:128580/1‑141 (MQ=255)
                                                                gTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAg                                                                         <  1:154017/141‑1 (MQ=255)
                                                                gTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAg                                                                         <  1:244795/141‑1 (MQ=255)
                                                                gTTTTCTCAATATCCAGAAAATTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAg                                                                         <  2:109741/141‑1 (MQ=255)
                                                                      tcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAttt                                                                   >  1:141197/1‑141 (MQ=255)
                                                                      tcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAttt                                                                   >  1:88120/1‑141 (MQ=255)
                                                                       cAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtc                                                                  >  1:166045/1‑141 (MQ=255)
                                                                        aaTATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtct                                                                 <  1:46549/141‑1 (MQ=255)
                                                                        aaTATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtct                                                                 <  1:997/141‑1 (MQ=255)
                                                                           atCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCaa                                                              <  1:339916/141‑1 (MQ=255)
                                                                           atCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCaa                                                              <  1:236839/141‑1 (MQ=255)
                                                                            tCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                             <  1:353582/141‑1 (MQ=255)
                                                                            tCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                             <  1:299534/141‑1 (MQ=255)
                                                                             ccAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGa                                                            <  2:131009/141‑1 (MQ=255)
                                                                                 aaaTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGATTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAACTAGATTTAGAGAAAAAAAAAAAAAAAAAGGAATTTCTCAAGAAACt                                                        <  2:294788/141‑1 (MQ=255)
                                                                                   aTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACttt                                                      <  1:341759/141‑1 (MQ=255)
                                                                                   aTTATCTTTAAAATTGGAAAACAATTTATTATGATAATTAATTACTATAAAAAAAATAAAAAATATACATCTAAAGATTAAAAAAAAGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACttt                                                      <  2:247047/141‑1 (MQ=255)
                                                                                       tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGt                                                  <  1:199798/141‑1 (MQ=255)
                                                                                        cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTa                                                 >  1:170234/1‑141 (MQ=255)
                                                                                               aTTGTAGCAAAATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAAGTTAAATTAATGAAAAATAGAATTAGAGAAAAAAAAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                          <  2:278249/141‑1 (MQ=255)
                                                                                                  gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                       <  1:123106/141‑1 (MQ=255)
                                                                                                  gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                       <  1:132344/141‑1 (MQ=255)
                                                                                                   tAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaaa                                      >  1:297705/1‑141 (MQ=255)
                                                                                                         tATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCt                                >  1:402437/1‑141 (MQ=255)
                                                                                                              cTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAAATAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                           <  2:128580/141‑1 (MQ=255)
                                                                                                               ttATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaa                          >  2:177737/1‑141 (MQ=255)
                                                                                                               ttATGCTGCTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACATTAAGTAAAGAAATAAAAGTATCTAGCCaa                          >  2:306465/1‑141 (MQ=255)
                                                                                                                 attcTGTTTAATTAATATACAAAAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAAc                        <  2:293083/138‑1 (MQ=255)
                                                                                                                 aTGCTGTTTAATTACTATAATAAAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAATCTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAAc                        <  2:356503/141‑1 (MQ=255)
                                                                                                                  tGCTGTTTAATTACTAAAATACAAATAAAAAATATACATCTAAAAGTTAAAATAATGAAAAATAGAATTAGAGAAAAAAAAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACt                       <  2:376015/141‑1 (MQ=255)
                                                                                                                    cTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                     >  1:16624/1‑141 (MQ=255)
                                                                                                                    cTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATCAAAAGTGTCTAGGCACACCAt                     >  1:227933/1‑141 (MQ=255)
                                                                                                                      gTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTa                   >  2:203236/1‑141 (MQ=255)
                                                                                                                      gTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTa                   >  2:191968/1‑141 (MQ=255)
                                                                                                                       tttAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTaa                  <  1:104986/141‑1 (MQ=255)
                                                                                                                          aaTTACTATACTACAAATAAAAAATATACATCTGAAGGTTAAATGAATGCAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGc               <  2:38355/141‑1 (MQ=255)
                                                                                                                          aaTTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGc               >  2:51927/1‑141 (MQ=255)
                                                                                                                           aTTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCa              >  2:46835/1‑141 (MQ=255)
                                                                                                                             tactatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt            >  1:284825/1‑141 (MQ=255)
                                                                                                                             tactatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt            >  2:404769/1‑141 (MQ=255)
                                                                                                                              actatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATa           >  1:145854/1‑141 (MQ=255)
                                                                                                                                 atactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATataa        <  2:378644/141‑4 (MQ=255)
                                                                                                                                 atactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATataa        <  2:363792/141‑4 (MQ=255)
                                                                                                                                 atactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaa        >  1:201415/1‑141 (MQ=255)
                                                                                                                                 atactaCAAATAAAAAATATACATCTAAAGGTTAAATTAAAGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaa        <  2:64909/141‑1 (MQ=255)
                                                                                                                                       cAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaat  >  1:290611/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                        
ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAAT  >  CP000731/25523‑25799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: