New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE = 273189428 (0.840)5 (0.150)
+ATTATAAACA
4/242 2.7 15.2% intergenic (‑22/‑111) USA300HOU_RS14060/cocE hypothetical protein/Cocaine esterase
?USA300TCH1516_ALE 2731895 = 30 (0.840)intergenic (‑23/‑110) USA300HOU_RS14060/cocE hypothetical protein/Cocaine esterase
Rejected: Frequency below/above cutoff threshold.

AAATCTGCAATTTGTTGCACAGTAATCTCTTCTAATTGGTGTTGATGTAAAAGTTCAATAAATGCATTTCTCATTGCAACTTGTGATTTTCTAATACGTCGATCTATAGTCATTTATATCAAGTCCTCCCCAATG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/2731760‑2731894
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTATAAACGTTATGTTCATTATCCCACAAATCTCCAACATTGATGATTGGCACACAATGTTTACCTGTTTAATATAGGT  >  USA300TCH1516_ALE/2731895‑2731974
                                                                                                                                       ||||||||||                                                                                
AAATCTGCAATTTGTTGCACAGTAATCTCTTCTAATTGGTGTTGATGTAAAAGTTCAATAAATGCATTTCTCATTGCAACTTGTGATTTTCTAATACGTCGATCTATAGTCATTTATATCAAGTCCTCCCCAATGATTATA                                                                                      <  1:143180/141‑1
      GCAATTTGTTGCACAGTAATCTCTTCTAATTGGTGTTGATGTAAAAGTTCAATAAATGCATTTCTCATTGCAACTTGTGATTTTCTAATACGTCGATCTATAGTCATTTATATCAAGTCCTCCCCAATGATTATAAACAAT                                                                                >  1:320248/1‑141
           TTGTTGCACAGTAATCTCTTCTAATTGGTGTTGATGTAAAAGTTCAATAAATGCATTTCTCATTGCAACTTGTGATTTTCTAATACGTCGATCTATAGTCATTTATATCAAGTCCTCCCCAATGATTATAAACAATTATAA                                                                           <  2:253202/141‑1
                                                                 ATTTCTCATTGCAACTTGTGATTTTCTAATACGTCGATCTATAGTCATTTATATCAAGTCCTCCCCAATGATTATAAACAATTATAAACGTTATGTTCATTATCCCACAAATCTCCAACATTGATGATTGGCACACAATGT                     >  1:13316/1‑141
                                                                                    GATTTTCTAATACGTCGATCTATAGTCATTTATATCAAGTCCTCCCCAATGATTATAAACAATTATAAACGTTATGTTCATTATCCCACAAATCTCCAACATTGATGATTGGCACACAATGTTTACCTGTTTAATATAGGT  <  2:320248/141‑1
                                                                                                                                       ||||||||||                                                                                
AAATCTGCAATTTGTTGCACAGTAATCTCTTCTAATTGGTGTTGATGTAAAAGTTCAATAAATGCATTTCTCATTGCAACTTGTGATTTTCTAATACGTCGATCTATAGTCATTTATATCAAGTCCTCCCCAATG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/2731760‑2731894
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTATAAACGTTATGTTCATTATCCCACAAATCTCCAACATTGATGATTGGCACACAATGTTTACCTGTTTAATATAGGT  >  USA300TCH1516_ALE/2731895‑2731974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.