Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE512,6430AG48.6% ‑5.3 / 44.0 35intergenic (+711/‑6052)recR/speA_2Recombination protein RecR/Arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (8/10);  new base G (7/10);  total (15/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGTAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATCAAACATCATA  >  USA300TCH1516_ALE/512505‑512782
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gtgtAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGATATTGACTTAAATGTtaa                                                                                                                                            >  2:225581/1‑140 (MQ=255)
     gttaagctccttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATaaaa                                                                                                                                       <  1:170910‑M1/30‑1 (MQ=255)
         agctccttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttcatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGta                                                                                                                                   >  1:140481‑M1/107‑140 (MQ=255)
          tACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGtat                                                                                                                                  >  1:39844/1‑140 (MQ=255)
                  ttAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATaattaatt                                                                                                                          <  1:256367/140‑1 (MQ=255)
                  gcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATaattaatt                                                                                                                          >  1:27863‑M1/98‑140 (MQ=255)
                       gatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGATGATAAaatgaataaataatatttgt                                                                                                                     <  2:176978‑M1/48‑7 (MQ=255)
                             agtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGATaa                                                                                                               <  1:223763‑M1/54‑1 (MQ=255)
                              gtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAg                                                                                                              >  1:173285‑M1/86‑140 (MQ=255)
                              aaaGCATGTATAGTAATTAATTTTTGAAGCAAATAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAAATAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAg                                                                                                              <  2:13305/140‑1 (MQ=255)
                                        taGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAAc                                                                                                    >  1:113010/1‑140 (MQ=255)
                                           gccgatagagtagaaagttgccaggcagtattttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACAtt                                                                                                 >  2:72877‑M1/73‑140 (MQ=255)
                                             cgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGa                                                                                               <  2:78024‑M1/70‑1 (MQ=255)
                                                  gagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACt                                                                                          <  1:119542‑M1/75‑1 (MQ=255)
                                                    gtagaacgtagacaggaagtttttaaatcgaattttggttaaaaaataaaatggacaagataaAAAAAGCTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGCACATTGAAAACTGa                                                                                        >  2:190899‑M1/64‑140 (MQ=255)
                                                      tgAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATATATACTTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGGAt                                                                                      >  2:72873/1‑140 (MQ=255)
                                                        aGGAAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGa                                                                                    <  2:174383/140‑1 (MQ=255)
                                                                   acaacTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTTAATAACAATATGTCaa                                                                         >  2:168263/1‑140 (MQ=255)
                                                                    caactcaactTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTACATATTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTTAAAACTGAATGACAatatctacat                                                                        >  2:40847/1‑134 (MQ=255)
                                                                      actcaactTAAGAAATAACATGAATGAAGAAGTAAAATTAATTTTAAAAAGTTATTAACTGAAAAGTTAATAAAATGTATAATTAATGCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGACAAAGt                                                                      >  2:131337/1‑140 (MQ=255)
                                                                            taatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTc                                                                <  1:22184‑M1/101‑1 (MQ=255)
                                                                                  aattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCaaaaa                                                          <  1:257457‑M1/107‑1 (MQ=255)
                                                                                        cTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTaa                                                    <  2:23918/140‑1 (MQ=255)
                                                                                         ttGAATTACTAACGAAAAATAATATAAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAAc                                                   <  2:28917/140‑1 (MQ=255)
                                                                                                aCTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGt                                            <  1:234842/140‑1 (MQ=255)
                                                                                                 ataaaaaaaaTAAATTATAAAAAGTTATTAACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAAAATTGAAAACTGAATGACAATATGTAAAAAAAAAAAAAAAAAAACGTAACTATAAGtt                                           <  2:258985/134‑1 (MQ=255)
                                                                                                    aCGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACa                                        <  1:259911/140‑1 (MQ=255)
                                                                                                    aCGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACa                                        >  2:82206/1‑140 (MQ=255)
                                                                                                        ggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACa                                    >  1:142307‑M1/12‑140 (MQ=255)
                                                                                                              attTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTAttt                              <  2:39844/140‑1 (MQ=255)
                                                                                                                 aaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGt                           >  1:78024‑M1/3‑140 (MQ=255)
                                                                                                                    aaaaGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTAtt                        <  1:175354/140‑1 (MQ=255)
                                                                                                                        gTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATACGTTACCAGCATTATTTAGTATTTATg                    >  1:232696/1‑140 (MQ=255)
                                                                                                                          tatcaaCTGAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAg                  <  1:67478/135‑1 (MQ=255)
                                                                                                                               aaatAAATGTTAATAAAATGTATAATTAATTCTTGTAGGTAAGAAAAATGAAAATTGAAAACTGAATGACAATATGTCAAAAATAAAAAAAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAAt             <  2:218255/137‑1 (MQ=18)
                                                                                                                                cgttaaTGTTGATAAAATGGATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAATACTGAATGACAATATGTAAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATc            <  2:99647/136‑1 (MQ=18)
                                                                                                                                          aataaAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAACCATTATTTAGTATTTATGAGATACTCCAACATCATa  >  2:48244/1‑140 (MQ=255)
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GTGTAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATCAAACATCATA  >  USA300TCH1516_ALE/512505‑512782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: