| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,519,951 | T→G | 11.3% | P227P (CCA→CCC) | narG ← | Respiratory nitrate reductase 1 alpha chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,519,951 | 0 | T | G | 11.3% | 76.0 / 2.5 | 38 | P227P (CCA→CCC) | narG | Respiratory nitrate reductase 1 alpha chain |
| Reads supporting (aligned to +/- strand): ref base T (21/11); new base G (3/2); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGATC > USA300TCH1516_ALE/2519815‑2520088 | cTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGACACATCAGTTTGCTCTCCCCAAATTTGTggca > 2:143229/1‑139 (MQ=255) aGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCCTTATTCCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAg < 1:307350/141‑1 (MQ=255) ggACTTCAGTCATAAAAGGTCTATCCGGAGTACTTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCATGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAggtg < 2:62574/141‑1 (MQ=255) aGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAACTTTGTGGAGAGGCAGCTGGTAAATc > 2:3785/1‑141 (MQ=255) aaTGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATAcc < 1:7338/141‑1 (MQ=255) cATCCGGAGTACGTGTTGAAGGTACATTATAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCAt < 2:287614/141‑1 (MQ=255) aatacGGAGTACTTTGTAAAGGTACAGTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCAt < 1:243271/137‑1 (MQ=255) gTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGGCATAAAAACTa > 1:195518/1‑141 (MQ=255) cGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTAAGGCACATCTGTTTGCTCTCCCCGAATTTCTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAACAACTAAGc > 2:93236/1‑141 (MQ=255) aaGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTcac > 1:220965/1‑141 (MQ=255) aTTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAg < 2:63703/141‑1 (MQ=255) tAGAGCTCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTCGCTCTCCCCAAATGGGTGGAGAGGCAGGTAGTAGATCTGCATACCAGTCATAAAAACTAAGGATTTCAGCACCAAGCa > 2:265229/1‑141 (MQ=255) gCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGGGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCGGCAGCAAGCAAAtt > 1:116473/1‑141 (MQ=255) ccACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTTTCCCCAAATTTGTGGACAGGCAGGTGGTAAATCTGCATACCAGTCACAAAAACTTACAATTTCACAACCAAGCCAATtgat > 2:147176/1‑141 (MQ=255) ataataATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAAt > 1:25306/1‑141 (MQ=255) ataatGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGa > 1:181636/1‑141 (MQ=255) taatGTATGATGCGTTATAACAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGCATCGaa > 2:337141/1‑140 (MQ=255) atggtgCGTTATACCAGTCAGTTGATTCGGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACctg < 2:55655/141‑1 (MQ=255) gatgCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACctgct > 1:308220/1‑141 (MQ=255) atgCGTTATACCAGTCACTTGATTCAGGCACATCTTTTTTTTTTTTTTTTTTTTATGCAGAGGCAGGTGCTAAATCTTCATACCAGTCATAAAAACTAAGCATTTCTCCACCACGCATCTTGATGAATCGAGCActtcctc > 2:195092/1‑135 (MQ=255) gCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCa > 2:30988/1‑141 (MQ=255) cTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGAc > 1:74393/1‑141 (MQ=255) tGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACAt > 1:290826/1‑141 (MQ=255) tGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACAt > 1:289131/1‑141 (MQ=255) aTTCAGGCACAGCGGGTGGCTCTCAGCATTTTTGGGGAGAGGCAGGTGGTAAATCTGCAGACCAGTCATAAAAACTAAGCATTTCACCACCAAGGAAATTGATGAACCGAGCAACTGCTGCATAACTAATAATTGACATCg < 2:167480/141‑1 (MQ=255) tCAGGCACATCTGTTTGCTCTCCCCCAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCt < 1:105889/141‑1 (MQ=255) acaagCACATCTGTTTGATCTCCCCAAATTTGTGAAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCt < 2:250009/137‑1 (MQ=255) cacaTCTGTTTGTTCTCACCACATCTACGCAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAAt < 1:264362/141‑1 (MQ=255) cTGTTTTCTCTCCCCAAAGTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGgtg < 1:291761/141‑1 (MQ=255) tGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCCTTCACAGCGCCGTAACAGgtgtccat > 2:274361/1‑137 (MQ=255) gCTCTCCCCAAAGTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGCATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATc < 1:95784/141‑1 (MQ=255) cccAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGa > 1:171296/1‑141 (MQ=255) cacAAATTTGGGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAAATAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCCTTACCATCGCTGGAATAGGTGTAAATCCTGCGa > 1:322/3‑141 (MQ=255) aaaTTTGGCGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTACGCATTTCACCAACAAGGAAATTGATGAATCGAGCACCTGCTGCATATCTAATCATTGACATCGCTCGAATAGGTGTAAATCCTGCGATTc < 2:220544/141‑1 (MQ=255) aTTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGa > 2:97201/1‑141 (MQ=255) aTTTGGGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGa > 1:179472/1‑141 (MQ=255) aTTTGGGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAACATTTCTCCACAAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGa > 1:178997/1‑141 (MQ=255) ttGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTACTCATTGCCATCGCTGGAATCGGTTTTAATACTGCGATTCGATc > 2:294882/1‑141 (MQ=255) | CTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGATC > USA300TCH1516_ALE/2519815‑2520088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |