Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073125,6630A.98.3% 721.3 / ‑2.4 159coding (316/333 nt)USA300HOU_pUSA300HOUMR0031hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base . (87/69);  minor base T (1/0);  total (88/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 168 total aligned reads displayed.

ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAAT  >  CP000731/25523‑25799
                                                                                                                                            |                                                                                                                                        
attgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATa                                                                                                                                          <  1:582181/141‑1 (MQ=255)
 ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaa                                                                                                                                         <  2:748761/141‑1 (MQ=255)
    tatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaa                                                                                                                                      <  1:887223/141‑1 (MQ=255)
    tatGTTATTTACACTAGTGCATAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaa                                                                                                                                      <  2:351928/141‑1 (MQ=255)
        ttATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatat                                                                                                                                  >  2:428680/1‑138 (MQ=255)
         tATTTACACTAGTGCTTAAATTTACAGGTTTGTCAACAAGGATGGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatata                                                                                                                                 <  1:531163/141‑5 (MQ=255)
         tATTTACACTAGTGCTTAAATTTACAGGTTTATCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATACTGTATAATTATTATACTACAAATaaaacatata                                                                                                                                 >  2:768871/1‑135 (MQ=255)
          atgtACACTAGTGATTAAATATACAAGTTAGACACCAAGTATTGAAGAATCTTGTTTTTCTCAATATCCAGAAATATTCTATATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATAc                                                                                                                               <  2:1002832/138‑1 (MQ=255)
          aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATAc                                                                                                                               >  1:398401/1‑141 (MQ=255)
          aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATAc                                                                                                                               >  1:280436/1‑141 (MQ=255)
           tttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACa                                                                                                                              >  1:689798/1‑141 (MQ=255)
            ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACAt                                                                                                                             >  2:717826/1‑141 (MQ=255)
            ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTAGTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACAt                                                                                                                             <  1:32669/141‑1 (MQ=255)
             tACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATc                                                                                                                            >  1:135540/1‑141 (MQ=255)
             tACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATc                                                                                                                            >  2:639979/1‑141 (MQ=255)
             tACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATc                                                                                                                            >  1:1026833/1‑141 (MQ=255)
                acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCACTCTCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaa                                                                                                                         >  2:124047/1‑141 (MQ=255)
                acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaa                                                                                                                         >  2:210463/1‑141 (MQ=255)
                acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAAGATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTCGTATAGTACAAATAAAAAATATACATTTaa                                                                                                                         >  2:89817/1‑141 (MQ=255)
                 cTATTGCTTAAATTTACAGGTGTGGCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaaa                                                                                                                        <  2:839253/141‑1 (MQ=255)
                 cTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaaa                                                                                                                        >  1:554431/1‑141 (MQ=255)
                   aGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTTTGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAgg                                                                                                                      >  1:811978/1‑141 (MQ=255)
                   aGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAgg                                                                                                                      <  2:327303/141‑1 (MQ=255)
                    gTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGt                                                                                                                     <  2:69166/141‑1 (MQ=255)
                    gTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGt                                                                                                                     <  2:276701/141‑1 (MQ=255)
                        ttAAATTTACAGGTTTGTCACCAAGTATTGAAGGATGTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGCTTaaa                                                                                                                 >  2:607996/1‑141 (MQ=255)
                         tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAAAATACTTTATAATTGTAGCACTCTTTCTTATGATGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTatct                                                                                                                >  1:146297/1‑138 (MQ=255)
                           aaTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAAGTGTATAACTAAATAATTGACGGCGTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTa                                                                                                              >  1:196298/1‑141 (MQ=255)
                            aTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTaa                                                                                                             <  1:430501/141‑1 (MQ=255)
                             tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATACTTATAACACTATTTCTTATGCTGTTTAATTACTTTACTACAAATAAAAAATATACATCTAAAGGTTAAATTAAt                                                                                                            <  1:143589/141‑1 (MQ=255)
                             tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAAt                                                                                                            >  1:1008852/1‑141 (MQ=255)
                             tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAAt                                                                                                            >  1:405924/1‑141 (MQ=255)
                                aCAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaa                                                                                                         >  2:292884/1‑141 (MQ=255)
                                aCAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATATTCTTTATCATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGGTAAATTAATGaa                                                                                                         >  2:266953/1‑141 (MQ=255)
                                  aGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaaaa                                                                                                       <  1:767125/141‑1 (MQ=255)
                                   ggTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGTTTAAATTAATGaaaaa                                                                                                      >  2:58528/1‑141 (MQ=255)
                                       tGTCACCAATTATTTAATGATCTTGGTTTTCTCAATATCCAAAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGa                                                                                                  <  1:367215/141‑1 (MQ=255)
                                            ccAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTag                                                                                             >  1:782169/1‑141 (MQ=255)
                                              aaGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTagag                                                                                           <  1:829511/141‑1 (MQ=255)
                                                ttATTAAAGGATATGTGTTTTATCAATATACAGAAATTGTCTATATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGaa                                                                                         <  2:241818/140‑1 (MQ=255)
                                                    tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                     >  2:384729/1‑141 (MQ=255)
                                                      aaGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                   >  1:1025583/1‑141 (MQ=255)
                                                           tCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCAATAAAAAAAGAAGTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACa                                                                              <  1:973463/141‑1 (MQ=255)
                                                             ttGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATCCAAAGATTCTGTAGATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaa                                                                            >  2:463531/1‑141 (MQ=255)
                                                             ttGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaa                                                                            >  1:590062/1‑141 (MQ=255)
                                                              tGGTTTTCTCAATATCCAGAACTTTTCTTTATAATTGTAGCACTAATTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATGTACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGATAATATATAaaaaaaaa                                                                           >  2:971596/1‑141 (MQ=255)
                                                              tGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaa                                                                           >  2:837316/1‑141 (MQ=255)
                                                               ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGTTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAAcaacaa                                                                          >  2:297170/1‑141 (MQ=255)
                                                               ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                          >  2:905898/1‑141 (MQ=255)
                                                               ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                          >  2:860824/1‑141 (MQ=255)
                                                                 ttttaacAATATACAGAAATTTTCTATATAATTGGAGCAAGATTTCTTATGCTGTTTAATTACTAGAATAGAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAgg                                                                        <  2:698704/135‑1 (MQ=255)
                                                                  tttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                       >  1:20596/1‑141 (MQ=255)
                                                                  tttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                       >  1:452891/1‑141 (MQ=255)
                                                                  tttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                       <  1:285579/141‑1 (MQ=255)
                                                                    tctcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAt                                                                     >  2:205700/1‑141 (MQ=255)
                                                                       cAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATAAAAATTAGATTTCGTTAATATATAAAACAAAAAGGAATTtc                                                                  <  1:978089/141‑1 (MQ=255)
                                                                       cAATATCCAGAAATTTTCTTTATAAGTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtc                                                                  <  1:259079/141‑1 (MQ=255)
                                                                       acatatACAGAAAATTTTATTATAGTTATAGCAATATTTATTATGCTGTTTTCCCTCTATCATACAAATAAAAAATCATCATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtc                                                                  <  2:315635/139‑1 (MQ=255)
                                                                             ccAGAAATTTTCTTTATAATTGTAGCACTATTTCTTTTGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGa                                                            <  2:811978/141‑1 (MQ=255)
                                                                                   atattCTATATAATTGTAGCAATAATTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACttt                                                      <  2:333584/138‑1 (MQ=255)
                                                                                    ttttCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTa                                                     <  2:402734/141‑1 (MQ=255)
                                                                                     tttCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAATAAACTTTaa                                                    >  1:454457/1‑141 (MQ=255)
                                                                                      ttCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTCCTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAg                                                   >  1:222072/1‑141 (MQ=255)
                                                                                          ttATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaaa                                               <  1:1001339/141‑1 (MQ=255)
                                                                                               aTTGGAGAAATATTTATAATGATGTGTAATTACTATACTAAAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                          <  2:676588/141‑1 (MQ=255)
                                                                                                  gTAGGACTAGTTGTTATGCGGTTTAATTAATATACTACAAATAAAAAATATAGATCGAAATGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                       <  2:384382/141‑1 (MQ=255)
                                                                                                  gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTata                                       >  1:267963/1‑139 (MQ=255)
                                                                                                  gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                       >  1:817695/1‑141 (MQ=255)
                                                                                                  gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                       >  1:1029557/1‑141 (MQ=255)
                                                                                                  gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                       >  1:721437/1‑141 (MQ=255)
                                                                                                   tAGCACTATTTCTTATGCTGTTTATTTGCAATAATACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaaa                                      <  1:434584/141‑1 (MQ=255)
                                                                                                   tAGCACTATTTCTTATGCTGTTTAATTACTATACTAAAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaaa                                      <  1:614695/141‑1 (MQ=255)
                                                                                                      cACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtg                                   <  1:97265/141‑1 (MQ=255)
                                                                                                           tttCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAATGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAATTCTCAAGAAACTTTAAGTAAAAAATTAAAAGTGTCTac                              >  2:444876/1‑140 (MQ=255)
                                                                                                           tttCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTTTATAg                              >  2:600144/1‑141 (MQ=255)
                                                                                                           tttCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                              <  1:56408/141‑1 (MQ=255)
                                                                                                           aggttgatgatgTTTAAAAACTAAACGACAAACAAAAAACATAAATAGAAAGGTGAAATGAATGAACAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                              <  2:210034/135‑1 (MQ=255)
                                                                                                            ttCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAgg                             >  1:490858/1‑141 (MQ=255)
                                                                                                              cTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAGGGAATTTCTCGAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                           >  2:571622/1‑141 (MQ=255)
                                                                                                               ttATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaa                          <  1:639979/141‑1 (MQ=255)
                                                                                                               ttATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaa                          >  1:178361/1‑141 (MQ=255)
                                                                                                                tATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaaa                         >  2:296567/1‑141 (MQ=255)
                                                                                                                    cTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                     <  2:280436/141‑1 (MQ=255)
                                                                                                                     tGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAtt                    >  1:64089/1‑141 (MQ=255)
                                                                                                                        ttAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAAt                 <  1:294410/141‑1 (MQ=255)
                                                                                                                        ttAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAAt                 <  1:359723/141‑1 (MQ=255)
                                                                                                                          aaTTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGc               <  2:782169/141‑1 (MQ=255)
                                                                                                                            ttACTATACTACAAATAAACAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGAAATTTCTCAAGAAACTCTCAGTAAAAAATTAAAAGTGTCGAGGCAAACTATTAATGCaa             >  2:499863/1‑141 (MQ=255)
                                                                                                                            ttACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa             >  2:946556/1‑141 (MQ=255)
                                                                                                                            ttACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa             >  2:247163/1‑141 (MQ=255)
                                                                                                                              actatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATa           <  1:776212/141‑1 (MQ=255)
                                                                                                                               ctatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAg          >  2:22986/1‑141 (MQ=255)
                                                                                                                                   actacAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaaaa      >  1:708563/1‑141 (MQ=255)
                                                                                                                                    ctacAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAATAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAAc     >  1:741436/1‑141 (MQ=255)
                                                                                                                                    ctacAAATAAAAAATATACATCTAAAAGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAAc     <  1:878878/141‑1 (MQ=255)
                                                                                                                                     aacAAATAAAAAATATACAACTAAAAGATAAATTAAAGAAAAATAGAATTAGAGAAAAAAAAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACa    <  2:726537/140‑1 (MQ=255)
                                                                                                                                       cAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaat  <  1:358125/141‑1 (MQ=255)
                                                                                                                                       cAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTATAGGCAAACTATTAATGCAATAGAAAACaaa  >  2:583796/1‑140 (MQ=255)
                                                                                                                                       cAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAATTCTAAAGAAACGATAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATACAATAGAAAACaat  >  2:118840/1‑141 (MQ=255)
                                                                                                                                        caaaaaaaaaaTATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaat  <  1:949091/137‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
ATTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAAT  >  CP000731/25523‑25799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: