New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 1965737 =103 (1.220)3 (0.040) 3/246 NT 2.9% coding (71/924 nt) hemH Ferrochelatase
?USA300TCH1516_ALE 1965782 = 101 (1.260)coding (26/924 nt) hemH Ferrochelatase
Rejected: Frequency below/above cutoff threshold.

CACATATACACAAAGATAATCATAGTTTTACATTGAAGCACATGAAAGATAATTCATTTAAAGGAAGGTATTATCAATGACTAAAAAAATGGGATTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGACATAGAGCCATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/1965883‑1965737
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agccataACTAATAATCCCATTTTTTTAGTCATTGATAATACCTTCCTTTAAATGAATTATCTTTCATGTGCTTCAATGTAAAACTATGATTATCTTTGTGTATATGTGTGTACGAATTCGCTTACTTTACGTAACGTCTCTGGTTG  >  USA300TCH1516_ALE/1965782‑1965921
                                                                                                                                                                                                                                                                                               
CACATATACACAAAGATAATCATAGTTTTACATTGAAGCACATGAAAGATAATTCATTTAAAGGAAGGTATTATCAATGACTAAAAAAATGGGATTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGACATAGA                                                                                                                                                    <  1:391186/141‑1
 ACATATACACAAAGATAATCATAGTTTTACATTGAAGCACATGAAAGATAATTCATTTAAAGGAAGGTATTATCAATGACTAAAAAAATGGGATTCTTCGTCAAGGCTTATGGCAAACCATATAAAGAAAGTGACATAGAG                                                                                                                                                   >  2:727375/1‑141
 ACATATACACAAAGATAATAATAGTTATACATTGAAGCAAATGAAAGATAATTCATTTAAAAGAAGGTATTATCAATGACTAAAAAAATGGGATTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGACATAGAG                                                                                                                                                   <  1:547674/141‑1
  CATATAGACAGATATAATCATAGTTTTACATTGAAGCACATGAAAGATAATTCATTTAAAGGAAGGTATTATCAATGACTAAAAAAATGGGATTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGACATAGAGC                                                                                                                                                  <  1:381603/141‑1
   ATATACAAAAATTAAATCATAGTTTTAAATTGAAGCAAATGAAAGATAATTCATTTAAAGGAAGGTATTATCAATGACTAAAAAAATGGGATTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGACATAGAGCC                                                                                                                                                 <  2:601117/141‑1
      TACACAAAGATAATCATAGTTTTACATTGAAGCACATGAAAGATAATTCATTTAAAGGAAGGTATTATCAATGACTAAAAAAATAGGATTATTAGTTATGGCTTATGGCACACCTTATAAAAAACGTAACATAGAGCCATA                                                                                                                                              >  2:664709/1‑141
                  ATCATAGTTTTACATTGAAGCACATGAAAGATAATTCATTTAAAGGAAGGTATTATCAATGACTAAAAAAATGGGATTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGACATAGAGCCATAACTAATAATCCC                                                                                                                                  >  1:251614/1‑141
                                                                                          GGGATTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGACATAGAGCCATAACTAATAATCCCATTTTTTTAGTCATTGATAATACCTTCATTTAAATGAATTATCTTTCATGTGCTTCAATGTAAAACTATGAT                                                          <  2:251614/141‑1
                                                                                                              ATGGCACACCTTATAAAGAAAGTGACATAGAGCCATAACTAATAATCCCATTTTTTTAGTCATTGATAATACCTTCCTTTAAATGAATTATCTTTCATGTGCTTCAATGTAAAACTATGATTATCTTTGTGTATATGTGTG                                      >  2:554521/1‑141
                                                                                                                                                ATAACTAATAATCCCATTTTTTTAGTCATTGATAATACCTTCCTTTAAATGAATTATCTTTCATGTGCTTCAATGTAAAACTATGATTATCTTTGTGTTTGTTTGTGTACGAATTCGCTTACTTTACGTAACGTCTCTGGT    >  1:34342/1‑141
                                                                                                                                                 TAACTAATAATCCCATTTTTTTAGTCATTGATAATACCTTCCTTTAAATGAATTATCTTTCATGTGCTTCAATGTAAAACTATGATTATCTTTGTGTATATTTGTGTTCAAATTTGCTTACTTTACGTACTGTCTCTGTTT   >  2:960375/1‑141
                                                                                                                                                 TAACTAATAATCCCATTTTTTTAGTCATTGATAATACCTTCCTTTAAATGAATTATCTTTCATGTACTTCAATGTACAACTATGATTATCTTTGTGTATATGTGTTTACGTCTTAGTTTACTTTATGTAACGTCTGTGGTT   >  2:639489/1‑141
                                                                                                                                                  AACTAATAATCCCATTTTTTTAGTCATTGATAATACCTTCCTTTAAATGAATTATCTTTCATGTGCTTCAATGTAAAACTATCATTATCTTTGTGTATATGTGTGTACGAATTCGCTTACTTTACGTAACGTCTCTGGTTG  <  1:522118/141‑1
                                                                                                                                                  AACGAATAATCACATTTTTTTAGTCATTGATAAGACCTTCAGTTAAATTAATTATCTTTCATGTGCTTCAATGTAAAACGATGATTATCTTTGTGTATATGTGTGTACGAATTCGCTTACTTTACGTAACGCCGCAGGTTG  <  2:25389/141‑1
                                                                                                                                                                                                                                                                                               
CACATATACACAAAGATAATCATAGTTTTACATTGAAGCACATGAAAGATAATTCATTTAAAGGAAGGTATTATCAATGACTAAAAAAATGGGATTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGACATAGAGCCATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/1965883‑1965737
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agccataACTAATAATCCCATTTTTTTAGTCATTGATAATACCTTCCTTTAAATGAATTATCTTTCATGTGCTTCAATGTAAAACTATGATTATCTTTGTGTATATGTGTGTACGAATTCGCTTACTTTACGTAACGTCTCTGGTTG  >  USA300TCH1516_ALE/1965782‑1965921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.