New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 2460492 =130 (1.540)5 (0.060) 3/246 NT 3.9% coding (783/900 nt) natA_2 ABC transporter ATP‑binding protein NatA
?USA300TCH1516_ALE 2460521 = 123 (1.540)coding (754/900 nt) natA_2 ABC transporter ATP‑binding protein NatA
Rejected: Frequency below/above cutoff threshold.

TATCAATCATGTACGTGCATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAACACTACCAGATTTAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGCAAGGTCTTCAGTTGACGATTGAGAATGAAGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/2460636‑2460492
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgaagacCTTGCTTCATGTTTTCACTATGAATGATGCCTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTGATTCAATGACAACTTTCTTGTTACCATTAGATGCACGTACATGATTGATATCACCAGAAACAACAAGTTGAC  >  USA300TCH1516_ALE/2460521‑2460658
                                                                                                                                                                                                                                                                                            
TATCAATCATGTACGTGCATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAACACTACCAGATTTAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGCAAGGTCTTCAGTTGACGATTGAGAATGA                                                                                                                                                <  1:248216/141‑1
    AATCATGTACGTGCATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAACACTACCAGATTTAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGCAAGGTCTTCAGTTGACGATTGAGAATGAAGAC                                                                                                                                            <  2:798752/141‑1
        ataTACGTGCATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAAAACTACCAGATTTAACAAACATTAGAGGCATCATTGATATTGAAAACATGAAGCAAGGTTTTCAGTTCACCAATAATACTGAAGACCTag                                                                                                                                        >  2:621729/4‑139
                 CATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAACACTACCAGATTTAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGCAAGGTCTTCAGTTGACGATTGAGAATGAAGACCTTGCTTCATGTT                                                                                                                               >  1:771254/1‑141
                 CATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAACACTACCAGATTTAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGCAAGGTCTTCATTCTCAATCGTCAACTGAAGACCTTGCTTCATGTT                                                                                                                               >  2:640408/1‑141
                 CATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAACACTACCAGATTTAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGCAAGGTCTTCATTCTCAATCGTCAACTGAAGACCTTGCTTCATGTT                                                                                                                               >  2:771254/1‑141
                 CATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAACACTACCAGATTTAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGAAAGGTCTACAGTTGACGATTGAGAATGAAAACCTTGCTTCATGTT                                                                                                                               >  1:640408/1‑141
                                                                       TAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGCAAGGTCTTCATTCTCAATCGTCAACTGAAGACCTTGCTTCATGTTTTCACTATGAATGATGCCTCTAATGTTTCTTCAATCTGGTAGTGTTGTTTCTGt                                                                         >  2:851889/1‑140
                                                                                                                                           TGAAGACCTTGCTTCATGTTTTCACTATGAATGATGCCTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTGATTCAATGACAACTTTCTTGTTACCATTAGATGCACGTACATGATTGATATCACCAGAAACAACAAGT      <  2:992425/141‑1
                                                                                                                                             acGACCTTGCTTTATGTTTTCACTATGAATGATGCCTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTTATTTAATTACAACTTTCTTGTTACCATTAGATGCACGTACATGATTGATATCACCAGAAACAACAAGTTG    <  1:974639/139‑1
                                                                                                                                             AAGACCTTGCTTCATGTTTTCACTATGAATGATGCCTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTGATTCAATGACAACTTTCTTGTTACCATTAGATGCACGTACATGATTGATATCACCAGAAACAACAAGTTG    <  1:51213/141‑1
                                                                                                                                              cGACCTTGCTTCATGTTTTCACTATGACTGATGCCTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTGATTCAATGACAACTTTCTTGTTACCATTAGATCCACGTCCATGATTGATATCACCAAAAACCACAACTTGA   >  2:266735/2‑141
                                                                                                                                              AGACCTTGCTTCATGAATTCACTATGAATGATGACTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTGATTCAATGACAACTTTCTTGTTACAATTATATGCACGTACATGATTGATATCATCAGAAACAACAAGTTGA   >  2:138560/1‑141
                                                                                                                                              AGACCTTGATTCATGTTTTCACTATGAATGATGCCTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTGATTCAATAACAACTTTCTTGTTACCATTAGATGCAGGTACATGATTGCTATCACAATAAACAACAActcga   >  2:656301/1‑136
                                                                                                                                               GACCTTGCTTCATGTTTTCACTATGAATGATGCCTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTGATTCAATGACAACTTTCTTGTTACCATTAGATGCACGTACATGATTGATATCACCAGAAACAACAAGTTGAC  >  1:820779/1‑141
                                                                                                                                                                                                                                                                                            
TATCAATCATGTACGTGCATCTAATGGTAACAAGAAAGTTGTCATTGAATCAGAAACAACACTACCAGATTTAACAAACATTAGAGGCATCATTCATAGTGAAAACATGAAGCAAGGTCTTCAGTTGACGATTGAGAATGAAGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/2460636‑2460492
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgaagacCTTGCTTCATGTTTTCACTATGAATGATGCCTCTAATGTTTGTTAAATCTGGTAGTGTTGTTTCTGATTCAATGACAACTTTCTTGTTACCATTAGATGCACGTACATGATTGATATCACCAGAAACAACAAGTTGAC  >  USA300TCH1516_ALE/2460521‑2460658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.