Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 111,560 T→C 8.1% R210R (CGT→CGC)  USA300HOU_RS00520 → putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE111,5600TC 8.1% 229.6 / 9.4 84R210R (CGT→CGC) USA300HOU_RS00520putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (43/34);  new base C (2/5);  total (45/39)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

TTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  USA300TCH1516_ALE/111420‑111699
                                                                                                                                             |                                                                                                                                           
tttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGt                                                                                                                                             <  1:378968/141‑1 (MQ=255)
  tAAGTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCGGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCaa                                                                                                                                           <  1:1003382/141‑1 (MQ=17)
   aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTaaa                                                                                                                                          <  1:891632/141‑1 (MQ=255)
   aaGGTTGTTGCGCAATATTGCAATTTTAAAGAGTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGTCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCaaa                                                                                                                                          <  2:856571/141‑1 (MQ=14)
    aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCaaaa                                                                                                                                         <  1:579238/141‑1 (MQ=255)
    aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCaaaa                                                                                                                                         <  1:596124/141‑1 (MQ=255)
     gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAg                                                                                                                                        <  2:1021573/141‑1 (MQ=255)
     gTTTTTTGCACAATATAGAAATTTGAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAg                                                                                                                                        <  2:364046/141‑1 (MQ=255)
         tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCAAAAGatat                                                                                                                                    >  1:947704/1‑141 (MQ=255)
         tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAATATAATCCAGAGGTTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAA‑AAATTTGTAAAatatat                                                                                                                                    >  2:614564/1‑141 (MQ=21)
         tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAT‑TAAAACTGTTTACGTACAAGctat                                                                                                                                    >  2:407754/1‑141 (MQ=21)
           tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTATCTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGa                                                                                                                                  <  1:396454/141‑1 (MQ=255)
              gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAACGATATGACAt                                                                                                                               >  2:78952/1‑141 (MQ=255)
               cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATa                                                                                                                              <  1:510442/141‑1 (MQ=255)
                     aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACcaaca                                                                                                                        >  2:772288/1‑141 (MQ=255)
                     aGCAATTATAAAGATTTAATGAGTTATAAAGATGGAGTTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCAAAAGATATGATATACcgacg                                                                                                                        <  2:484868/141‑2 (MQ=11)
                      gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCAAAAGATATGATATAccgacga                                                                                                                       >  1:471970/1‑136 (MQ=11)
                      gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACcaacaa                                                                                                                       >  2:25050/1‑141 (MQ=255)
                       aaaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACaaa                                                                                                                      <  1:104187/140‑1 (MQ=255)
                           tttAAAGATTTAATGAATTATAACGATGTATATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                  <  1:645425/141‑1 (MQ=255)
                           tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                  <  1:826851/141‑1 (MQ=255)
                           tttAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                  <  1:918191/141‑1 (MQ=255)
                              aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               <  2:336681/141‑1 (MQ=255)
                                aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTACTACAGCATCAAATATAATAC‑AATTACGTAAACGATATGACATACCAACACATACAGCAcc                                                                                                             >  2:114892/1‑141 (MQ=17)
                                aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                                                             <  2:936351/141‑1 (MQ=255)
                                   tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                                                                          <  1:1016730/141‑1 (MQ=255)
                                   tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                                                                          <  1:106147/141‑1 (MQ=255)
                                   tttAATGAATTATAAAGATGGAGATAGATCATATAATCCAGAGGTGCCGAGTTCTCCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                                                                          <  1:751277/141‑1 (MQ=255)
                                     tAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACAAACAAATAAAACGCAGAAGc                                                                                                        >  2:739295/1‑141 (MQ=255)
                                       aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGcag                                                                                                      >  1:737042/1‑139 (MQ=255)
                                       aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                      <  1:219192/141‑1 (MQ=255)
                                       aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                      >  1:35441/1‑141 (MQ=255)
                                          aagtataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                                                                   <  2:902894/138‑1 (MQ=255)
                                           aTTATAAAGATGGAGATATATCATATAATCCAGCAATGACGAGTTATTCAGCTCAATATCAATTAAATAATATTGATGATAATGTCCCTT‑TATGACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACtgttga                                                                                                  >  1:916196/1‑140 (MQ=17)
                                               taAATATGGAGATATTTTATATAATCTAGATGTTTCTAGTTCTACAGTTGGTTATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaa                                                                                              <  2:728163/141‑1 (MQ=14)
                                                aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACCAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                                                                             <  1:593328/141‑1 (MQ=255)
                                                  aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                                                                           <  1:635123/141‑1 (MQ=255)
                                                  aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                                                                           <  1:491273/141‑1 (MQ=255)
                                                   gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgga                                                                                          >  1:742511/1‑140 (MQ=255)
                                                   gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                                                                          >  1:266763/1‑141 (MQ=255)
                                                        agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                                                                     <  1:336444/141‑1 (MQ=255)
                                                        agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGCAAAGGTACAgg                                                                                     >  2:110289/1‑141 (MQ=255)
                                                         aatatagCAGATAATGCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCTCCGAAGCTGTTGTTGAAAGGTACAgtg                                                                                    <  2:863951/140‑3 (MQ=14)
                                                                     aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                        <  1:77691/141‑1 (MQ=255)
                                                                      aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAACGCCGAAGCTGTTGTTGAAAGGTACAGGAACTTACAAAGGtt                                                                       >  2:585352/1‑141 (MQ=17)
                                                                       tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                      >  2:47313/1‑141 (MQ=255)
                                                                       tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACTGTTGTTAAAAAGTACAAGGAATTTAAAAGGTtc                                                                      >  2:235919/1‑141 (MQ=255)
                                                                        ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTACTGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  1:25045/1‑141 (MQ=255)
                                                                        ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  1:245986/1‑141 (MQ=255)
                                                                        ccAGAGGTGCCGAGATATTCAGCTGAATATCAATGAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     <  1:106177/141‑1 (MQ=255)
                                                                         cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGAAATGACATACCAACAAATAAAGCGCCGAAACTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                                                                    >  2:623811/1‑141 (MQ=255)
                                                                          agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATAACAACAAATAAAGCGCCGAAGCTGTTGTTGCAAGGTATAGGGAATTTAACAGGTtcata                                                                   >  1:278185/1‑140 (MQ=255)
                                                                           gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑ACTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGCACAGGGAATTTCAAAGGTtcatca                                                                  >  2:55705/1‑141 (MQ=255)
                                                                               ggacGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg                                                              <  2:385783/138‑1 (MQ=255)
                                                                                        aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaa                                                     >  1:27496/1‑141 (MQ=255)
                                                                                          tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGAGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa                                                   >  1:163722/1‑141 (MQ=255)
                                                                                           cAGCTCAATATCAATTAACTAATGCTGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGACTTTAAACGGTTCATCAGTTGGATATaacaaaa                                                  >  1:179494/1‑141 (MQ=255)
                                                                                                  atatCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAtt                                           >  1:411995/1‑141 (MQ=255)
                                                                                                     tCAATTAACTAATGATGATTATAATGTAAAAA‑AAATACGTAAAAGATATGATATACCAAAAAATAAAGCGACGTAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTTATCAGTTGGATATAAAAAAATTGAATTTAc                                        <  2:582315/141‑1 (MQ=17)
                                                                                                         ttAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtttt                                    >  1:383069/1‑141 (MQ=255)
                                                                                                          tAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACttttt                                   >  2:182998/1‑141 (MQ=255)
                                                                                                          tAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACTGTTGTTGAAAGGTACAGGGAATTTAAAAGATTCATCAGTTGACTATCAAAACATTAAATTTACtttct                                   >  2:795560/1‑139 (MQ=14)
                                                                                                          tAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAACGCAGAAGCTGTTGTTGAAAGCTACAAAGAATTTAAACGGTTCATCAGTTGGATAAAAAAAAATTGCATTTACtttct                                   >  2:284951/1‑139 (MQ=17)
                                                                                                           aaCTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtttttt                                  >  1:541686/1‑141 (MQ=255)
                                                                                                           aaCTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCAGTTGATAAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTAGATATAAAAAAATTGAATTTACtttctt                                  >  2:679216/1‑141 (MQ=17)
                                                                                                            aCTAATGATGATTCTAACGTAAAAC‑ACTAACGTAAAAGATAAGACATACAAAAAAATAAATCGCCGATGCTGACGTTAGAAGGTACAGGGAATTTAAAAGGTTCAACAGTCGCATATAAAAAAATTGACTTTACtcttcga                                 >  2:949855/1‑138 (MQ=12)
                                                                                                            aCTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTa                                 >  2:827679/1‑141 (MQ=255)
                                                                                                            aCTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTa                                 >  2:825251/1‑141 (MQ=255)
                                                                                                              caaTGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTaga                               <  1:629333/140‑1 (MQ=255)
                                                                                                                 tgatgaTTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                            <  1:25050/141‑1 (MQ=255)
                                                                                                                       ttATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                      >  1:268230/1‑141 (MQ=255)
                                                                                                                       atataATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAAATGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGACTaaaaa                      >  2:172785/2‑141 (MQ=255)
                                                                                                                        tataATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAAt                     >  2:971736/1‑141 (MQ=255)
                                                                                                                          aaaTGTAAAAC‑AATTATGTAAAAGATATGACATAACAACAAATAAAGCGCAGAAGCTGATGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGa                   <  2:415705/140‑1 (MQ=255)
                                                                                                                          aaaTGTAAAAC‑AATTACGTAAAAGATATGACATAGCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGa                   <  2:492684/140‑1 (MQ=255)
                                                                                                                           aaTGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa                  >  1:280012/1‑141 (MQ=255)
                                                                                                                              gTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAat               >  1:807875/1‑141 (MQ=255)
                                                                                                                                 aaaC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtt            <  1:713498/141‑1 (MQ=255)
                                                                                                                                     aaatgaagTAAAAGATATGACATACAAAAAAAGAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac         <  2:843781/134‑1 (MQ=18)
                                                                                                                                     aaaTTACGTAAAAGATATTACATAACAACAAATAAAACGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac         <  2:583902/140‑1 (MQ=255)
                                                                                                                                       aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactt       >  2:375352/1‑141 (MQ=255)
                                                                                                                                           cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAGAAATAATATTATttactttact   >  2:407952/1‑141 (MQ=255)
                                                                                                                                           cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact   <  1:740424/141‑1 (MQ=255)
                                                                                                                                            gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttactt  >  2:432305/1‑140 (MQ=255)
                                                                                                                                            gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACATGTTTAGAGAACAACAACAAAAATTTTTACTTTACTg  >  2:885492/1‑141 (MQ=17)
                                                                                                                                             |                                                                                                                                           
TTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  USA300TCH1516_ALE/111420‑111699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑