Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,479 T→C 10.0% D1014D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4790TC10.0% 255.9 / 4.2 113D1014D (GAT→GACsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (35/61);  new base C (9/4);  total (44/69)
Fisher's exact test for biased strand distribution p-value = 3.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

ATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAC  >  USA300TCH1516_ALE/622340‑622617
                                                                                                                                           |                                                                                                                                           
     gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                                                                                      <  1:125487/141‑1 (MQ=255)
     gaCAGCGACTCAGATTAAGATAGAGACTAAGATTCGGACAGCGATTCAGACTGAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                                                                                      <  2:716602/141‑1 (MQ=255)
       cAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc                                                                                                                                    <  1:625196/141‑1 (MQ=255)
        aGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACACAGATAGCGACTCAGATTCAGAGAGCGATTCGGCCTCAGATAGCGACTCAGAGTCa                                                                                                                                   >  2:681030/1‑141 (MQ=11)
           gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCGGAt                                                                                                                                <  1:994272/141‑1 (MQ=255)
             tccacattcagatGGCGACTCAGATGCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                              <  2:857868/136‑1 (MQ=255)
             gccagatgcagaaAGCGAAGCAGATTCAGACAGCGACGGAGATTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                              <  2:336126/139‑1 (MQ=11)
               cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                                            >  1:42712/1‑141 (MQ=255)
                 gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                          <  1:317524/141‑1 (MQ=255)
                   ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                                        <  1:793090/141‑1 (MQ=255)
                   ttcagatAGCGACGCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                                        <  1:476170/141‑1 (MQ=255)
                     cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                                                      >  1:599871/1‑141 (MQ=255)
                     cagatACCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                                                      <  1:221916/141‑1 (MQ=255)
                        atAGCGACTCAGAATCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagact                                                                                                                   <  1:744533/141‑1 (MQ=255)
                          aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                                                                                                                 <  1:392753/141‑1 (MQ=255)
                          aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                                                                                                                 <  1:680143/141‑1 (MQ=255)
                           gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACACAGACAGCGATACAGAGTCCGACAGCGacgcagactcag                                                                                                                >  2:523573/1‑141 (MQ=11)
                           gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATACAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATGCGGACTCAGACAGCGATTCAGACTCAGAGAGCGacccagacccag                                                                                                                >  2:882055/1‑141 (MQ=14)
                                tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATACCGACTCAGACTcagccagc                                                                                                           >  2:312658/1‑141 (MQ=14)
                                acAGATGCGGACAGCGAGGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGc                                                                                                           <  2:162443/140‑1 (MQ=255)
                                 cAGATTCGGACAGCGATCCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGACAGCGCTTCAGACTCAGATAGCGACTCAGACTCAGACAGCg                                                                                                          <  1:201055/141‑1 (MQ=255)
                                   gactCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                        <  1:331411/138‑1 (MQ=255)
                                   gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATCGCGCCTCGGACTCAGACAGCGAc                                                                                                        >  1:685676/1‑141 (MQ=255)
                                   gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                        <  1:736508/141‑1 (MQ=255)
                                   gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                        <  1:716282/141‑1 (MQ=255)
                                    atccagACAGCGACTCAGACGCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACt                                                                                                       <  2:867018/136‑1 (MQ=14)
                                     ttCAGACAGCCATTTATACTGAGATAGAGACTACGATGCAGACAGCGAATCAGAGTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc                                                                                                      <  2:676338/141‑1 (MQ=11)
                                       cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                                    >  1:367886/1‑141 (MQ=255)
                                       cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                                    <  1:565684/141‑1 (MQ=255)
                                        ggAGAGCTACTCATACTCATAAAGCGACGCTGTGTCTGACATCGTTTCATACTCTGACAGCTACTCGGATTCAGATAGCGATTCCGACTCAGACAATGTTAATACCGCTTATAGCGACTCAGACTCAGACAGCGACtcaga                                                                                                   <  2:470845/141‑1 (MQ=9)
                                             gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCCGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                              >  2:118877/1‑141 (MQ=255)
                                                    ggACTCAGACAGTGACTCGGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                                                                                       <  2:1023471/140‑1 (MQ=11)
                                                     gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACACAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                                      >  1:950053/1‑141 (MQ=255)
                                                      aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGaat                                                                                     >  1:442021/1‑139 (MQ=255)
                                                      aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAtt                                                                                     >  1:768438/1‑141 (MQ=255)
                                                        tCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCg                                                                                   >  1:72218/1‑141 (MQ=255)
                                                        tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAACGACTCACACTCAGACAGCGACTAAGATGACGATAGCGACTcc                                                                                   >  2:853115/1‑140 (MQ=14)
                                                        tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGAATca                                                                                   >  2:961811/1‑140 (MQ=255)
                                                         aaGATAGCGACTCAGAGTCAGAGAGCGATTCAGACGCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                                                                  <  1:382277/140‑1 (MQ=255)
                                                              aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAACGACTCAGACACAAACAACGACTCAGACTCAGAAACCAACTCGGACtaa                                                                             >  2:365144/1‑139 (MQ=11)
                                                                cGACTCAGATTCAGATAGCGAGTAAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCACATCCACATAGCAACTCACACTCaaa                                                                           >  2:751029/1‑139 (MQ=11)
                                                                 gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGCATCACACTCTGACAGCGACTCGGCCTCacaa                                                                          >  2:60217/1‑138 (MQ=11)
                                                                    tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                       <  1:553723/141‑1 (MQ=255)
                                                                     cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                      >  1:931525/1‑141 (MQ=255)
                                                                     cagattaagatAGCGATTCAGACTCAGACAGCGACTCAGATGCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                      <  1:132891/141‑1 (MQ=255)
                                                                      agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCCGACAGCGa                                                                     >  2:337617/1‑141 (MQ=11)
                                                                           cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGATACAGACTCAGACAGCGACTCaa                                                                >  2:885428/1‑140 (MQ=11)
                                                                           cagagAGGGATTCAGCATCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAGAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                <  2:42712/141‑1 (MQ=14)
                                                                             gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCCAGaa                                                              >  2:754532/1‑140 (MQ=14)
                                                                                agggaatcagactgagatAGAGACTGATATTAAGACAGCGATAGGGAATCAGAGAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCa                                                           <  2:284120/135‑1 (MQ=14)
                                                                                aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGCCTCAGacaca                                                           >  2:333866/1‑137 (MQ=14)
                                                                                    aTTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACa                                                       <  1:735621/141‑1 (MQ=255)
                                                                                     ttCAGTCCCAGACCGCGACTCAGCTTCAGATAGCGATTCGGACTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                      <  2:599871/141‑1 (MQ=14)
                                                                                         gactcaggtCGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                  <  2:148919/141‑1 (MQ=255)
                                                                                          actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                 >  1:557388/1‑141 (MQ=255)
                                                                                          actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTTAGATAGCGACTCAGAGTCGGTCAGCGagt                                                 >  1:674402/1‑139 (MQ=14)
                                                                                            ccagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                               <  1:950273/140‑1 (MQ=255)
                                                                                              aaacAGCGACTCAGATTCAGATAGCGACTCGGACTCAGAGAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                             <  2:177145/139‑1 (MQ=255)
                                                                                               gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                            <  1:882055/141‑1 (MQ=255)
                                                                                               gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                            <  1:939196/141‑1 (MQ=255)
                                                                                                 cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                          <  1:343150/141‑1 (MQ=255)
                                                                                                     gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAAACCAAAGACTAAATCGTTTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                      >  1:751545/1‑141 (MQ=17)
                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGCTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    <  1:541075/141‑1 (MQ=255)
                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    <  1:559104/141‑1 (MQ=255)
                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    <  1:814072/141‑1 (MQ=255)
                                                                                                       cTCAGATTCAAATAGCAATTCGGACTCAGACAGCGATTCCGACTCAGAGAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    <  1:968881/141‑1 (MQ=255)
                                                                                                          agattcagatATCTATTCGGCCTCAGACGCCGATTCAGACTCAGATAGCGCCTCAGACTCAGTCACCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga                                 <  1:285019/141‑1 (MQ=17)
                                                                                                           gatgcagacAGCGACGCCGACGCAGACAGCGATTCCGAGTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAAAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                <  2:472632/141‑1 (MQ=14)
                                                                                                             ttcatatCGCGATTCGGACTCAGACGGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                              <  1:493088/141‑1 (MQ=255)
                                                                                                             ttcagacAACGAGGCAGACACAGACAGCGATTCAGACACAGATAGCGACTCAGAGTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                              <  2:454050/141‑1 (MQ=11)
                                                                                                               cagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTAAGACTCAGATAGCgactcag                            >  1:868960/1‑141 (MQ=255)
                                                                                                                 gatGGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                          <  1:70248/141‑1 (MQ=255)
                                                                                                                 gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                          <  1:392453/141‑1 (MQ=255)
                                                                                                                 gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAAACAGCGAATCATGCTCTGATAGCcactcagac                          >  1:139357/1‑141 (MQ=17)
                                                                                                                 gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCCGAGCGCgactcacac                          >  2:244965/1‑141 (MQ=14)
                                                                                                                   ttgagATTCCGGCTCAGACAGCGACCCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATTCGGGCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCAAAGATCtactcagactc                        <  2:376887/137‑1 (MQ=11)
                                                                                                                   tAGAGATGCGGGCTAAGACGGCGACCCAGACGCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAAAGCGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                        <  2:804897/141‑1 (MQ=11)
                                                                                                                   caGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                        <  1:515599/140‑1 (MQ=255)
                                                                                                                   aaGCGAGTCGGACTCAGACAGGGAGTGAGACTCAGATAGCGACTCACACCCAGAAAGACACTCAGACTCAGACAGCGACTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                        <  2:592160/140‑1 (MQ=11)
                                                                                                                         ttCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAACCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCACACTACGATAGCGCCTCAGACCCAGACAg                  >  2:559824/1‑141 (MQ=14)
                                                                                                                          tCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  1:289001/141‑1 (MQ=255)
                                                                                                                          acagaagcAGAAAGTGAATAAGACTAAGATAGCGACTCAGACGCAGAAAACGACTCAGATTCAGATAGCGACTCTGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  2:152751/134‑1 (MQ=14)
                                                                                                                           cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                >  1:683242/1‑141 (MQ=255)
                                                                                                                           cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                >  1:626692/1‑141 (MQ=255)
                                                                                                                           cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGTg                >  1:728459/1‑141 (MQ=255)
                                                                                                                            ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTga               >  2:681404/1‑141 (MQ=255)
                                                                                                                             gagtCACACAGCGATTCAGACGCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgat              <  1:898591/138‑1 (MQ=255)
                                                                                                                             gACTCAGACAGCGATGCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgat              <  1:83756/141‑1 (MQ=255)
                                                                                                                              aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCGGATAGCGACTCAGAATCGAACAGTgaga             >  2:285133/1‑139 (MQ=14)
                                                                                                                                 cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGCTTCGGACTCAGATAGCGACTCAGAATCAGACAGCGATTCAGACCCAGATAACGACTCAGACTCAGACAgagacccac          >  2:271716/1‑136 (MQ=17)
                                                                                                                                     cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTGAGATAGCGACTCGGACTCAGATAGAGACACAGATGCAGACAGAGATTCAGATTCAGATAACGACTCAGACTCAGACAGAgaatcagatga      >  2:869922/1‑139 (MQ=14)
                                                                                                                                     cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc      >  1:402066/1‑141 (MQ=255)
                                                                                                                                     cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc      >  1:999834/1‑141 (MQ=255)
                                                                                                                                     cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGAGACTCAGACTCAGACAGTgattcagattc      >  1:72626/1‑141 (MQ=255)
                                                                                                                                     cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTAAGATACAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACATCGATTCAGACTCAGATAGCGACACAGACTCAGACAGTgattcagatta      >  1:96607/1‑140 (MQ=255)
                                                                                                                                        cGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga   <  1:999842/141‑1 (MQ=255)
                                                                                                                                        cGATTCAGACGCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga   <  1:126555/141‑1 (MQ=255)
                                                                                                                                         gATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAc  <  1:128702/141‑1 (MQ=255)
                                                                                                                                         gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAc  >  1:419313/1‑141 (MQ=255)
                                                                                                                                         gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAc  <  1:131196/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAC  >  USA300TCH1516_ALE/622340‑622617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: