Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,479 T→C 10.0% D1014D (GAT→GAC)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4790TC10.0% 255.9 / 4.2 113D1014D (GAT→GAC) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (35/61);  new base C (9/4);  total (44/69)
Fisher's exact test for biased strand distribution p-value = 3.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

ATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAC  >  USA300TCH1516_ALE/622340‑622617
                                                                                                                                           |                                                                                                                                           
     gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑c                                                                                                                                      <  1:125487/141‑1 (MQ=255)
     gaCAGCGACTCAGATTAAGATAGAGACTAAGATTCGGACAGCGATTCAGACTGAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑c                                                                                                                                      <  2:716602/141‑1 (MQ=255)
       cAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTc                                                                                                                                    <  1:625196/141‑1 (MQ=255)
        aGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACACAGATAGCGACTCAGATTCAGAGAGCGATTCGGCCTCAGATAGCGACTCAGA‑GTCa                                                                                                                                   >  2:681030/1‑141 (MQ=11)
           gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCGGAt                                                                                                                                <  1:994272/141‑1 (MQ=255)
             tccacattcagatGGCGACTCAGATGCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg                                                                                                                              <  2:857868/136‑1 (MQ=255)
             gccagatgcagaaAGCGAAGCAGATTCAGACAGCGACGGAGATTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg                                                                                                                              <  2:336126/139‑1 (MQ=11)
               cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCg                                                                                                                            >  1:42712/1‑141 (MQ=255)
                 gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgac                                                                                                                          <  1:317524/141‑1 (MQ=255)
                   ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactc                                                                                                                        <  1:793090/141‑1 (MQ=255)
                   ttcagatAGCGACGCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactc                                                                                                                        <  1:476170/141‑1 (MQ=255)
                     cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcag                                                                                                                      >  1:599871/1‑141 (MQ=255)
                     cagatACCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcag                                                                                                                      <  1:221916/141‑1 (MQ=255)
                        atAGCGACTCAGAATCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagact                                                                                                                   <  1:744533/141‑1 (MQ=255)
                          aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagactca                                                                                                                 <  1:392753/141‑1 (MQ=255)
                          aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagactca                                                                                                                 <  1:680143/141‑1 (MQ=255)
                           gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACACAGACAGCGATACAGA‑GTCCGACAGCGacgcagactcag                                                                                                                >  2:523573/1‑141 (MQ=11)
                           gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATACAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATGCGGACTCAGACAGCGATTCAGA‑CTCAGAGAGCGacccagacccag                                                                                                                >  2:882055/1‑141 (MQ=14)
                                tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATACCGACTCAGACTcagccagc                                                                                                           >  2:312658/1‑141 (MQ=14)
                                acAGATGCGGACAGCGAGGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGc                                                                                                           <  2:162443/140‑1 (MQ=255)
                                 cAGATTCGGACAGCGATCCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGACAGCGCTTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCg                                                                                                          <  1:201055/141‑1 (MQ=255)
                                   gactCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                        <  1:331411/138‑1 (MQ=255)
                                   gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATCGCGCCTCGGACTCAGACAGCGAc                                                                                                        >  1:685676/1‑141 (MQ=255)
                                   gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                        <  1:736508/141‑1 (MQ=255)
                                   gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                        <  1:716282/141‑1 (MQ=255)
                                    atccagACAGCGACTCAGACGCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACt                                                                                                       <  2:867018/136‑1 (MQ=14)
                                     ttCAGACAGCCATTTATACTGAGATAGAGACTACGATGCAGACAGCGAATCAGAGTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtc                                                                                                      <  2:676338/141‑1 (MQ=11)
                                       cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                                    >  1:367886/1‑141 (MQ=255)
                                       cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                                    <  1:565684/141‑1 (MQ=255)
                                        ggAGAGCTACTCATACTCATAAAGCGACGCTGTGTCTGACATCGTTTCATACTCTGACAGCTACTCGGATTCAGATAGCGATTCCGACTCAGACAATG‑TTAATACCGCTTATAGCGACTCAGACTCAGACAGCGACtcaga                                                                                                   <  2:470845/141‑1 (MQ=9)
                                             gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCCGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                              >  2:118877/1‑141 (MQ=255)
                                                    ggACTCAGACAGTGACTCGGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGA‑TTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                                                                                       <  2:1023471/140‑1 (MQ=11)
                                                     gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACACAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                                      >  1:950053/1‑141 (MQ=255)
                                                      aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGaat                                                                                     >  1:442021/1‑139 (MQ=255)
                                                      aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAtt                                                                                     >  1:768438/1‑141 (MQ=255)
                                                        tCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCg                                                                                   >  1:72218/1‑141 (MQ=255)
                                                        tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAACGACTCACACTCAGACAGCGACTAAGATGACGATAGCGACTcc                                                                                   >  2:853115/1‑140 (MQ=14)
                                                        tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGAATca                                                                                   >  2:961811/1‑140 (MQ=255)
                                                         aaGATAGCGACTCAGAGTCAGAGAGCGATTCAGACGCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                                                                  <  1:382277/140‑1 (MQ=255)
                                                              aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATAACGACTCAGACACAAACAACGACTCAGACTCAGAAACCAACTCGGACtaa                                                                             >  2:365144/1‑139 (MQ=11)
                                                                cGACTCAGATTCAGATAGCGAGTAAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCACATCCACATAGCAACTCACACTCaaa                                                                           >  2:751029/1‑139 (MQ=11)
                                                                 gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGCATCACACTCTGACAGCGACTCGGCCTCacaa                                                                          >  2:60217/1‑138 (MQ=11)
                                                                    tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                       <  1:553723/141‑1 (MQ=255)
                                                                     cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                      >  1:931525/1‑141 (MQ=255)
                                                                     cagattaagatAGCGATTCAGACTCAGACAGCGACTCAGATGCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                      <  1:132891/141‑1 (MQ=255)
                                                                      agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCCGACAGCGa                                                                     >  2:337617/1‑141 (MQ=11)
                                                                           cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGATACAGACTCAGACAGCGACTCaa                                                                >  2:885428/1‑140 (MQ=11)
                                                                           cagagAGGGATTCAGCATCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGAGAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                <  2:42712/141‑1 (MQ=14)
                                                                             gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCCAGaa                                                              >  2:754532/1‑140 (MQ=14)
                                                                                agggaatcagactgagatAGAGACTGATATTAAGACAGCGATAGGGAATCAGAGAGCGAGTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCa                                                           <  2:284120/135‑1 (MQ=14)
                                                                                aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGCCTCAGacaca                                                           >  2:333866/1‑137 (MQ=14)
                                                                                    aTTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACa                                                       <  1:735621/141‑1 (MQ=255)
                                                                                     ttCAGTCCCAGACCGCGACTCAGCTTCAGATAGCGATTCGGACTCAGACAGCGAGTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                      <  2:599871/141‑1 (MQ=14)
                                                                                         gactcaggtCGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                  <  2:148919/141‑1 (MQ=255)
                                                                                          actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                 >  1:557388/1‑141 (MQ=255)
                                                                                          actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTTAGATAGCGACTCAGAGTCGGTCAGCGagt                                                 >  1:674402/1‑139 (MQ=14)
                                                                                            ccagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                               <  1:950273/140‑1 (MQ=255)
                                                                                              aaacAGCGACTCAGATTCAGATAGCGACTCGGACTCAGAGAGCGACTCAGA‑CTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                             <  2:177145/139‑1 (MQ=255)
                                                                                               gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                            <  1:882055/141‑1 (MQ=255)
                                                                                               gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                            <  1:939196/141‑1 (MQ=255)
                                                                                                 cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                          <  1:343150/141‑1 (MQ=255)
                                                                                                     gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAAACCAAAGACTAAATCGTTTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                      >  1:751545/1‑141 (MQ=17)
                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGCTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    <  1:541075/141‑1 (MQ=255)
                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    <  1:559104/141‑1 (MQ=255)
                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    <  1:814072/141‑1 (MQ=255)
                                                                                                       cTCAGATTCAAATAGCAATTCGGACTCAGACAGCGATTCCGA‑CTCAGAGAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    <  1:968881/141‑1 (MQ=255)
                                                                                                          agattcagatATCTATTCGGCCTCAGACGCCGATTCAGA‑CTCAGATAGCGCCTCAGACTCAGTCACCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga                                 <  1:285019/141‑1 (MQ=17)
                                                                                                           gatgcagacAGCGACGCCGACGCAGACAGCGATTCCGA‑GTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAAAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                <  2:472632/141‑1 (MQ=14)
                                                                                                             ttcatatCGCGATTCGGACTCAGACGGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                              <  1:493088/141‑1 (MQ=255)
                                                                                                             ttcagacAACGAGGCAGACACAGACAGCGATTCAGA‑CACAGATAGCGACTCAGAGTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                              <  2:454050/141‑1 (MQ=11)
                                                                                                               cagatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTAAGACTCAGATAGCgactcag                            >  1:868960/1‑141 (MQ=255)
                                                                                                                 gatGGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                          <  1:70248/141‑1 (MQ=255)
                                                                                                                 gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                          <  1:392453/141‑1 (MQ=255)
                                                                                                                 gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAAACAGCGAATCATGCTCTGATAGCcactcagac                          >  1:139357/1‑141 (MQ=17)
                                                                                                                 gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCCGAGCGCgactcacac                          >  2:244965/1‑141 (MQ=14)
                                                                                                                   ttgagATTCCGGCTCAGACAGCGACCCAGA‑CTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATTCGGGCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCAAAGATCtactcagactc                        <  2:376887/137‑1 (MQ=11)
                                                                                                                   tAGAGATGCGGGCTAAGACGGCGACCCAGA‑CGCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAAAGCGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                        <  2:804897/141‑1 (MQ=11)
                                                                                                                   caGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                        <  1:515599/140‑1 (MQ=255)
                                                                                                                   aaGCGAGTCGGACTCAGACAGGGAGTGAGA‑CTCAGATAGCGACTCACACCCAGAAAGACACTCAGACTCAGACAGCGACTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                        <  2:592160/140‑1 (MQ=11)
                                                                                                                         ttCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAACCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCACACTACGATAGCGCCTCAGACCCAGACAg                  >  2:559824/1‑141 (MQ=14)
                                                                                                                          tCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  1:289001/141‑1 (MQ=255)
                                                                                                                          acagaagcAGAAAGTGAATAAGA‑CTAAGATAGCGACTCAGACGCAGAAAACGACTCAGATTCAGATAGCGACTCTGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  2:152751/134‑1 (MQ=14)
                                                                                                                           cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                >  1:683242/1‑141 (MQ=255)
                                                                                                                           cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                >  1:626692/1‑141 (MQ=255)
                                                                                                                           cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGTg                >  1:728459/1‑141 (MQ=255)
                                                                                                                            ggACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTga               >  2:681404/1‑141 (MQ=255)
                                                                                                                             gagtCACACAGCGATTCAGA‑CGCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgat              <  1:898591/138‑1 (MQ=255)
                                                                                                                             gACTCAGACAGCGATGCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgat              <  1:83756/141‑1 (MQ=255)
                                                                                                                              aCTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCGGATAGCGACTCAGAATCGAACAGTgaga             >  2:285133/1‑139 (MQ=14)
                                                                                                                                 cAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGCTTCGGACTCAGATAGCGACTCAGAATCAGACAGCGATTCAGACCCAGATAACGACTCAGACTCAGACAgagacccac          >  2:271716/1‑136 (MQ=17)
                                                                                                                                     cAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTGAGATAGCGACTCGGACTCAGATAGAGACACAGATGCAGACAGAGATTCAGATTCAGATAACGACTCAGACTCAGACAGAgaatcagatga      >  2:869922/1‑139 (MQ=14)
                                                                                                                                     cAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc      >  1:402066/1‑141 (MQ=255)
                                                                                                                                     cAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc      >  1:999834/1‑141 (MQ=255)
                                                                                                                                     cAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGAGACTCAGACTCAGACAGTgattcagattc      >  1:72626/1‑141 (MQ=255)
                                                                                                                                     cAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTAAGATACAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACATCGATTCAGACTCAGATAGCGACACAGACTCAGACAGTgattcagatta      >  1:96607/1‑140 (MQ=255)
                                                                                                                                        cGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga   <  1:999842/141‑1 (MQ=255)
                                                                                                                                        cGATTCAGA‑CGCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga   <  1:126555/141‑1 (MQ=255)
                                                                                                                                         gATTCAGA‑CTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAc  <  1:128702/141‑1 (MQ=255)
                                                                                                                                         gATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAc  >  1:419313/1‑141 (MQ=255)
                                                                                                                                         gATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAc  <  1:131196/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAC  >  USA300TCH1516_ALE/622340‑622617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑