Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 683,202 T→A 6.0% Y23F (TAT→TTT)  ftsL_1 ← Cell division protein FtsL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE683,2020TA 6.0% 288.3 / 8.6 101Y23F (TAT→TTT) ftsL_1Cell division protein FtsL
Reads supporting (aligned to +/- strand):  ref base T (44/50);  new base A (3/3);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 102 total aligned reads displayed.

CATCAGAAATAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACTTTAAAA  >  USA300TCH1516_ALE/683065‑683342
                                                                                                                                         |                                                                                                                                              
catcaGAAATAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCGAATCTTCAATTTGATTTTGTTTCATTGTAACTTGTTGTTGAGGAATCTGATatc                                                                                                                                             >  1:85260/1‑139 (MQ=255)
catcaGAAATAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGGTacc                                                                                                                                             >  1:329926/1‑139 (MQ=255)
  tcaGAAATAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCa                                                                                                                                           <  2:206542/141‑1 (MQ=255)
  tcaGAAATAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCa                                                                                                                                           <  1:672885/141‑1 (MQ=255)
     taaaTAACTGTTTTTTATTGTCTTTTTACTAATTTATTTATCTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAAtt                                                                                                                                        <  2:452194/140‑1 (MQ=255)
     gAAATAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAAtt                                                                                                                                        <  1:594850/141‑1 (MQ=255)
     gAAATAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAAtt                                                                                                                                        <  1:787440/141‑1 (MQ=255)
     gAAATAACTGTTTTTGATTGTCTCTTTACTAAATCATGTAACCCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAAtt                                                                                                                                        <  2:504934/141‑1 (MQ=255)
     aaaaTAACTGTTTTTGATTGTACCTTTACTTATTACAGTATCTCTCCATTTTTATCTGTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACGTGTTGTTTAGGAATCTGATAACCAAtt                                                                                                                                        <  2:724282/140‑1 (MQ=255)
       aaTAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTaa                                                                                                                                      >  2:640719/1‑141 (MQ=255)
          aaCTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTTATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTaaaca                                                                                                                                   <  1:411232/141‑3 (MQ=255)
          aaCTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAAc                                                                                                                                   <  2:439315/141‑1 (MQ=255)
          aaCTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAAc                                                                                                                                   >  1:817286/1‑141 (MQ=255)
           aCTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACa                                                                                                                                  >  1:221197/1‑141 (MQ=255)
           aCTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACa                                                                                                                                  >  1:663894/1‑141 (MQ=255)
           aCTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTAATTTTGTTTCATTTTAACTTGTTTTTTAGTAATCTGATAACCTATTAAAACa                                                                                                                                  >  2:415456/1‑141 (MQ=255)
           aCTGTTTTTGATTGTCTCTTTACTAATTTATGTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTCGTTTAGGAATCTGATAACCAATTAAAACa                                                                                                                                  >  2:863524/1‑141 (MQ=255)
            cTGTTTTTGATTGTCTCTTTACTAATTTATTTAGCTCTGCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAg                                                                                                                                 <  2:26357/141‑1 (MQ=255)
             tGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGc                                                                                                                                <  2:261877/141‑1 (MQ=255)
             tGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAAGAATCGTATAACCAATTAAAACatc                                                                                                                                <  2:473404/141‑3 (MQ=255)
                   tGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACaaaa                                                                                                                          <  2:143320/141‑1 (MQ=255)
                      ttGTCTCTTTACTAATTTATTTTACTATCCTTTTTTATCTCTTAATATACATTGTTCTTCCTGCAAATCTTCAATTTGATTTTCTTTCATTTTAATTTGTTGTTTAGGAATCTGATAACCAACTAAAACAGCACAAactaa                                                                                                                      >  2:127811/1‑141 (MQ=255)
                                  tAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTTTTATAAATTTATATGTTCAATTTGATTTTGTTACAATTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTAc                                                                                                          <  2:114880/141‑1 (MQ=255)
                                  tAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTAc                                                                                                          >  2:788051/1‑141 (MQ=255)
                                  tAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTAc                                                                                                          <  2:608346/141‑1 (MQ=255)
                                  tAATTTATTTAACTCTCCATATTTATCTCTTAATATACGTCGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTATTTAGAAATTTAATAACCAATTAAAACAGCACAAAATAATATTACTGTTAc                                                                                                          >  2:519898/1‑141 (MQ=255)
                                   tatttatttAACTCTCCATTTTTATCTCTTAATAGACGTTGTTCTTCCTGCAAATCTGCAATTTGATTTTGTTTCATTTTAACTGGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAGATAATATTACTGTTACa                                                                                                         <  1:269446/140‑1 (MQ=255)
                                     tttatttaACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAag                                                                                                       >  2:438073/1‑141 (MQ=255)
                                     tttatttaACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAag                                                                                                       >  2:1027847/1‑141 (MQ=255)
                                     tttatttaACTCTCCATTTTTATCTCCTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAag                                                                                                       >  2:493292/1‑141 (MQ=255)
                                       tatttaACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGTAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAagag                                                                                                     <  2:373548/141‑1 (MQ=255)
                                         tttaACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTa                                                                                                   >  1:252641/1‑141 (MQ=255)
                                         tttaACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTa                                                                                                   >  1:579367/1‑141 (MQ=255)
                                           taACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTGATGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTaaa                                                                                                 >  1:315682/1‑141 (MQ=255)
                                           taACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTaaa                                                                                                 <  2:957554/141‑1 (MQ=255)
                                           taACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTaaa                                                                                                 >  1:665291/1‑141 (MQ=255)
                                             aCTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACa                                                                                               >  2:961034/1‑141 (MQ=255)
                                                 tcCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACgtaat                                                                                           >  1:216669/1‑141 (MQ=255)
                                                 tcCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACataat                                                                                           <  1:1006546/141‑1 (MQ=255)
                                                  ccATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGACTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACataata                                                                                          <  1:715032/141‑1 (MQ=255)
                                                   cATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACAtaataa                                                                                         <  2:120775/141‑1 (MQ=255)
                                                   cATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACAtaataa                                                                                         >  1:236173/1‑141 (MQ=255)
                                                     tttttATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAAtt                                                                                      <  1:733309/141‑1 (MQ=255)
                                                     tttttATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAAtt                                                                                      <  1:640719/141‑1 (MQ=255)
                                                      ttttATCTCTTAATATACGTTGTTCTTCCTGCAAATATTCAATTTGGTTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAACATAATCTTACGGGTACAAGAGTAAACATAATAAttt                                                                                     <  2:18780/141‑1 (MQ=255)
                                                        ttATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAAttttt                                                                                   <  1:160811/141‑1 (MQ=255)
                                                        ttATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAAttttt                                                                                   <  1:840123/141‑1 (MQ=255)
                                                        ttATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAAATTATAATTTATGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAAttttt                                                                                   <  2:624144/141‑1 (MQ=255)
                                                         tATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAAtatttt                                                                                  >  1:906416/1‑141 (MQ=255)
                                                          aTCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTATAACAGAACAAAATAATATTACTGTTACACGAGTAAACATAATAAttttttt                                                                                 >  2:154934/1‑140 (MQ=255)
                                                          aTCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATACTATTACTGTTACAAGAGTCCACATAAAAGTTTTTTg                                                                                 >  2:613288/1‑141 (MQ=255)
                                                          aTCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTg                                                                                 <  1:264827/141‑1 (MQ=255)
                                                          aTCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTATTATTTAGGAATATGATAATCAATTAAAACAGCACAAAATAATATTACTTTTACAAAATTAAACAACATAAtatttta                                                                                 >  2:489075/1‑140 (MQ=255)
                                                           tctcTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACTAGAGTAAACATAATAAtttttttc                                                                                >  2:853510/1‑139 (MQ=255)
                                                           tctcTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGc                                                                                >  2:538638/1‑141 (MQ=255)
                                                             tctTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGAAAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCtt                                                                              >  1:403080/1‑141 (MQ=255)
                                                             tctTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGAAAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCtt                                                                              >  1:43967/1‑141 (MQ=255)
                                                             tctTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGAAAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCtt                                                                              >  1:45160/1‑141 (MQ=255)
                                                              tttAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTc                                                                             <  2:765758/140‑1 (MQ=255)
                                                              tttAATATACGTTGTTCTTACTTGAAATATTAAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTc                                                                             <  2:114121/140‑1 (MQ=255)
                                                                  atatACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCattt                                                                         <  2:465529/141‑1 (MQ=255)
                                                                     taCGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCatttatt                                                                      >  1:629433/1‑141 (MQ=255)
                                                                         ttgttCTTCTTGCATAACAACAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCa                                                                  <  2:249347/141‑1 (MQ=255)
                                                                          tgttCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCAc                                                                 >  2:127621/1‑141 (MQ=255)
                                                                          tgttCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATACGAAACAAAATTGATCAACAGTATTTCTATTTTTCTGTTACAAGAGTAACCATAATAATTTTTTGCTTCATTTATTTTCAc                                                                 >  2:221223/1‑141 (MQ=255)
                                                                            ttcttcCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTc                                                               >  1:656778/1‑141 (MQ=255)
                                                                              cttcCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTTTTTTTTTTTTTTTCactact                                                             >  2:837063/1‑141 (MQ=255)
                                                                              cttcCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCt                                                             >  1:516059/1‑141 (MQ=255)
                                                                               ttcCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCtt                                                            >  1:171637/1‑141 (MQ=255)
                                                                                    gCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTCCTTCATTTATTTTCACTCCTTATCAt                                                       >  1:80738/1‑141 (MQ=255)
                                                                                     cAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATg                                                      >  1:4043/1‑141 (MQ=255)
                                                                                        aTCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAAt                                                   <  2:817286/141‑1 (MQ=255)
                                                                                        aTCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAAt                                                   <  1:830865/141‑1 (MQ=255)
                                                                                        aTCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAAt                                                   >  2:579908/1‑141 (MQ=255)
                                                                                          cTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCTTTTATTTTCACTACTTATCATGATTaa                                                 >  2:302871/1‑141 (MQ=255)
                                                                                             cAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAAc                                              <  2:752770/141‑1 (MQ=255)
                                                                                             cAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAAc                                              <  1:841167/141‑1 (MQ=255)
                                                                                                    aTTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATcc                                       <  2:49662/141‑1 (MQ=255)
                                                                                                     ttttGTTTCATTTTAACTTGTTGTTTAGGAATCTGATATCCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCg                                      <  1:438073/141‑1 (MQ=255)
                                                                                                     ttttGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCg                                      <  1:493292/141‑1 (MQ=255)
                                                                                                     ttttGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCg                                      <  1:1027847/141‑1 (MQ=255)
                                                                                                         gTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCgtcag                                  >  1:614804/1‑141 (MQ=255)
                                                                                                                     cTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATg                      <  1:579908/141‑1 (MQ=255)
                                                                                                                       tgttgtTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCGCTCCTTATCCTGAATAAAACTATATCCGTCAGTCATTTTCTATGTa                    >  1:538619/1‑141 (MQ=255)
                                                                                                                       tgttgtTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTa                    >  2:829723/1‑141 (MQ=255)
                                                                                                                           gttTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGtt                >  1:80383/1‑141 (MQ=255)
                                                                                                                           gttTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGtt                >  1:59236/1‑141 (MQ=255)
                                                                                                                              tAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCAt             <  1:106996/141‑1 (MQ=255)
                                                                                                                                tgAATCTAATAACCAATTAAAACAGTAAAAAATAATATTACTTTTACAAGATTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTa           <  2:693531/140‑1 (MQ=255)
                                                                                                                                ataaTCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTa           <  2:664337/139‑1 (MQ=255)
                                                                                                                                 gAATCTGATAACCAATTAAAAAAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTa           <  2:243661/141‑1 (MQ=255)
                                                                                                                                   aTCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACt        <  1:872435/141‑1 (MQ=255)
                                                                                                                                    tCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACtt       >  1:350290/1‑141 (MQ=255)
                                                                                                                                    tCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACtt       <  2:236173/141‑1 (MQ=255)
                                                                                                                                    tCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACtt       <  1:1011837/141‑1 (MQ=255)
                                                                                                                                     cTGAAAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACttt      <  2:45160/141‑1 (MQ=255)
                                                                                                                                     cTGAAAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACttt      <  2:43967/141‑1 (MQ=255)
                                                                                                                                     cTGAAAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACttt      <  2:403080/141‑1 (MQ=255)
                                                                                                                                        aTAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTAATTTTAAATGTAAGTTCATTAAATTTaaa   >  1:346998/1‑141 (MQ=255)
                                                                                                                                         tAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTCCATAGAATAAGTGTTTGCTTCATTTATTGTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACTTTaaaa  >  1:287196/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                              
CATCAGAAATAACTGTTTTTGATTGTCTCTTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAAAATAATATTACTGTTACAAGAGTAAACATAATAATTTTTTGCTTCATTTATTTTCACTCCTTATCATGAATAAAACTATATCCGTCAGTCATTTTCAATGTACGTTCATTAACTTTAAAA  >  USA300TCH1516_ALE/683065‑683342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: