Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,021,533 T→C 5.5% D201G (GAT→GGT)  ugtP ← Processive diacylglycerol beta‑glucosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,021,5330TC 5.5% 320.5 / 6.1 109D201G (GAT→GGT) ugtPProcessive diacylglycerol beta‑glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (59/44);  new base C (3/3);  total (62/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.68e-01

Only 100 of 109 total aligned reads displayed.

ACGCTTTAGCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTGTTTCGTTTCTTT  >  USA300TCH1516_ALE/1021397‑1021669
                                                                                                                                        |                                                                                                                                        
aCGCTTTAGCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATCTATCAGTAATCATCGTGTCAAAACCTTTAGATACACGAAATGCACCAGCTGACATTAACCTATTTTGCTTATCTgg                                                                                                                                      >  2:937083/1‑141 (MQ=255)
  gCTTTAGCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGAt                                                                                                                                    >  1:84268/1‑141 (MQ=255)
      tAGCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTACTTATCTGGATCTaa                                                                                                                                >  1:527981/1‑141 (MQ=255)
       aGCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTACCTGGATCTAAg                                                                                                                               >  2:37688/1‑141 (MQ=255)
       aGCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTACCTCGTTCCAAg                                                                                                                               >  2:871771/1‑141 (MQ=255)
       aGCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATAATCGTGTCAAAACCTTTAGATACAATAAATGCACAAGATGACATTAAAATAGTTTGCTTACATAGATCTAAg                                                                                                                               >  2:394721/1‑141 (MQ=255)
        tctctTTCCTCTTACCACAAATCATAACTACTTTTTCATTTGCACTTTTTGCTAATATATCAGTAATCATCTTGTCAAAACCTTTAGATACACCAAATGCACCAGCTTACATTAAAATATTTTGCTTATATGTATCTAAgt                                                                                                                              <  2:20380/140‑1 (MQ=255)
        gCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAgt                                                                                                                              <  1:247692/141‑1 (MQ=255)
        gCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAgt                                                                                                                              <  1:793147/141‑1 (MQ=255)
        gCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAgt                                                                                                                              <  2:81819/141‑1 (MQ=255)
          tctTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCCGCAGTTGCCATTAAAATAGTTTTCTTCTCTGGGTCTCAgttt                                                                                                                            >  1:967399/1‑140 (MQ=255)
             ttGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGttgttg                                                                                                                         <  1:840479/141‑1 (MQ=255)
                 tctTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTa                                                                                                                     >  2:689539/1‑141 (MQ=255)
                 tctTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTa                                                                                                                     >  1:498943/1‑141 (MQ=255)
                 tctTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTAATTATTTGGATCTAAGTTGTTGTCtt                                                                                                                     >  1:911266/1‑140 (MQ=255)
                  ctTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTAt                                                                                                                    >  2:830349/1‑141 (MQ=255)
                   ttACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTAtt                                                                                                                   >  2:598553/1‑141 (MQ=255)
                       cacaAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAAcc                                                                                                               <  2:958877/141‑1 (MQ=255)
                         caAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCAc                                                                                                             <  1:499039/141‑1 (MQ=255)
                         caAATCATAACTACTTGTGCATCTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCAc                                                                                                             <  1:497334/141‑1 (MQ=255)
                          aaaTCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACt                                                                                                            <  2:143783/141‑1 (MQ=255)
                           aaTGATAGCTACGTGTGCATTGGCACTTTTCGCTAATAGCTCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAGTGCACCAGCTGACTGTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTg                                                                                                           <  2:631226/141‑1 (MQ=255)
                           aaTCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTg                                                                                                           >  1:835204/1‑141 (MQ=255)
                           aaTCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTg                                                                                                           <  1:472258/141‑1 (MQ=255)
                           aaTCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTg                                                                                                           <  1:518926/141‑1 (MQ=255)
                           aaTCATAACTACTTGTGCACTTGCTCCTTTCGCTAACTTATCAGTACTCATTGTTTCAAAACCTTTAGATACACCAAATTCCCCAGCTGATATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTg                                                                                                           <  2:21092/141‑1 (MQ=255)
                            aTCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGc                                                                                                          >  1:365551/1‑141 (MQ=255)
                            aTCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGc                                                                                                          >  1:872566/1‑141 (MQ=255)
                            aTCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGc                                                                                                          >  1:565193/1‑141 (MQ=255)
                            aTCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGc                                                                                                          >  2:33461/1‑141 (MQ=255)
                             tCATAACTTGTTGTGCATTTGGAGTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCt                                                                                                         <  2:877985/141‑1 (MQ=255)
                             tCATAACTACTTGTGCATGTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTGGATACACCAAATGCTCCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCt                                                                                                         <  2:438835/141‑1 (MQ=255)
                                 aaCTACTTGTGCATTTGCACTTTTCGCAAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACAAAATGCACAAGCTGACATAAAAATAGTTTGCTTATATGTAACCAAGTTGTTATCTATTAACCACTGCTTTTg                                                                                                     >  2:8969/1‑141 (MQ=255)
                                    tactTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTTAtt                                                                                                  >  1:133854/1‑141 (MQ=255)
                                    tactTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGAtt                                                                                                  <  1:780452/141‑1 (MQ=255)
                                    taattGTGCATTTGCACTTTTCGCTCATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAGATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTCCAACAGACTGCTTTTGAtt                                                                                                  <  2:900553/138‑1 (MQ=255)
                                          tgCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAgg                                                                                            >  2:448328/1‑141 (MQ=255)
                                           gCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAGCTACTGCTGTTGATTAATAGGc                                                                                           >  1:643087/1‑141 (MQ=255)
                                           gCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATCGGc                                                                                           >  2:585612/1‑141 (MQ=255)
                                           gCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACAACTGCTTTTGATTAATAGGc                                                                                           >  1:497580/1‑141 (MQ=255)
                                              tttGCACTTTTTGATAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGtt                                                                                        <  2:486092/141‑1 (MQ=255)
                                                   aCTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCaaa                                                                                   <  2:609339/141‑1 (MQ=255)
                                                      tttCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCCAAttt                                                                                >  1:497349/1‑141 (MQ=255)
                                                      tttCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAAttt                                                                                <  1:195621/141‑1 (MQ=255)
                                                      tttCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAAttt                                                                                >  2:502738/1‑141 (MQ=255)
                                                       ttCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTg                                                                               <  1:127109/141‑1 (MQ=255)
                                                          gCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGTCGTTTCCAATTTGTTa                                                                            >  2:393086/1‑141 (MQ=255)
                                                           cTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAACATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAACTTTGtttt                                                                           >  2:532162/1‑139 (MQ=255)
                                                             aaTATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCCCTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCa                                                                         <  1:1004560/141‑1 (MQ=255)
                                                                atatCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATa                                                                      <  2:415371/141‑1 (MQ=255)
                                                                    cAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGaa                                                                  >  2:680890/1‑141 (MQ=255)
                                                                          tcatcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTg                                                            <  2:359540/141‑1 (MQ=255)
                                                                          tcatcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTg                                                            <  1:466573/141‑1 (MQ=255)
                                                                          tcatcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTg                                                            <  1:689539/141‑1 (MQ=255)
                                                                          tcatcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTg                                                            >  1:978453/1‑141 (MQ=255)
                                                                          tcatcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTg                                                            <  1:355271/141‑1 (MQ=255)
                                                                          tcatcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTg                                                            <  1:355298/141‑1 (MQ=255)
                                                                           catcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATAAATGGGCGTTTCAAATTTGTTATCAATAGGAACACCTGt                                                           >  1:798322/1‑141 (MQ=255)
                                                                            atcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCACATTTGTTATCAATAGAAATACCTGTc                                                          >  2:713066/1‑141 (MQ=255)
                                                                            atcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTc                                                          <  1:312558/141‑1 (MQ=255)
                                                                            atcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTc                                                          <  1:205858/141‑1 (MQ=255)
                                                                            atcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTACCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTc                                                          <  1:394721/141‑1 (MQ=255)
                                                                            atcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTACCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTc                                                          <  1:871771/141‑1 (MQ=255)
                                                                            atcGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTACCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTc                                                          <  1:37688/141‑1 (MQ=255)
                                                                              cGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCAc                                                        >  1:166945/1‑141 (MQ=255)
                                                                               gtgtCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTACTAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACt                                                       >  2:32060/1‑141 (MQ=255)
                                                                                 gtCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACttt                                                     >  1:835777/1‑141 (MQ=255)
                                                                                    aaaaCCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAAc                                                  <  1:103813/141‑1 (MQ=255)
                                                                                     aaaCCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAATTTTTTTATCAATAGGAATACCTGTCACTTTAACt                                                 >  2:584246/1‑141 (MQ=255)
                                                                                      aaCCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTg                                                <  2:445432/141‑1 (MQ=255)
                                                                                      aaCCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTg                                                <  2:453318/141‑1 (MQ=255)
                                                                                      aaCCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTg                                                <  2:738512/141‑1 (MQ=255)
                                                                                        ccTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGtt                                              >  1:778555/1‑141 (MQ=255)
                                                                                         cTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATATAAGGTTGTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTg                                             >  1:653767/1‑141 (MQ=255)
                                                                                                       aaTGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCt                               >  1:681307/1‑141 (MQ=255)
                                                                                                        aTGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATCCCTa                              >  2:814549/1‑141 (MQ=255)
                                                                                                         tGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTGTCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTaa                             >  1:895235/1‑140 (MQ=255)
                                                                                                         tGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTAc                             >  1:1000161/1‑141 (MQ=255)
                                                                                                              cAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTa                        >  1:208209/1‑141 (MQ=255)
                                                                                                              cAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTa                        <  2:365551/141‑1 (MQ=255)
                                                                                                              cAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTa                        <  2:872566/141‑1 (MQ=255)
                                                                                                              cAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTa                        >  2:54872/1‑141 (MQ=255)
                                                                                                                    aCATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGt                  >  2:151404/1‑141 (MQ=255)
                                                                                                                     cATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTc                 >  1:374376/1‑141 (MQ=255)
                                                                                                                     cATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTc                 >  1:365209/1‑141 (MQ=255)
                                                                                                                       ttAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATATCTACGTCTATGAAGTCtt               >  2:480820/1‑141 (MQ=255)
                                                                                                                       ttAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCtt               >  1:378503/1‑141 (MQ=255)
                                                                                                                       ttAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCtt               >  2:625057/1‑141 (MQ=255)
                                                                                                                       ttAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCtt               >  1:359019/1‑141 (MQ=255)
                                                                                                                        tAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTTTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTg              <  2:596445/141‑1 (MQ=255)
                                                                                                                        tAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTATATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTg              <  1:88859/141‑1 (MQ=255)
                                                                                                                          aaaTAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATATGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTtttt            >  2:792225/1‑141 (MQ=255)
                                                                                                                           aaTAGTTTGCTTATCTGGATGTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTgttt           <  2:521596/141‑1 (MQ=255)
                                                                                                                            aTAGTTTGCTTATTTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTgtttc          <  1:341655/141‑1 (MQ=255)
                                                                                                                              aGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTgtttcgt        <  1:255230/141‑1 (MQ=255)
                                                                                                                               gTTTGCTTATCTGGCTCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGCTCAATACCTACGTCTATGAAGTCTTgtttcgtt       >  1:352422/1‑141 (MQ=255)
                                                                                                                               gTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTgtttcgtt       <  2:309058/141‑1 (MQ=255)
                                                                                                                                   gCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGTTTTTGGTCAATAGGCGTTTCAAATTTGTTATCAATAGTTATAAATGTCATTTTAACTTTTGAAGTATCAATACATACGTCTATGAATTCTTGTTTCGTTTCtt   >  2:417253/1‑141 (MQ=255)
                                                                                                                                   gCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTGTTTCGTTTCttt  >  2:333998/1‑141 (MQ=255)
                                                                                                                                    tttATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTGTTTCGTTTCttt  <  2:527981/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ACGCTTTAGCTCTTTGCTCTTACCACAAATCATAACTACTTGTGCATTTGCACTTTTCGCTAATATATCAGTAATCATCGTGTCAAAACCTTTAGATACACCAAATGCACCAGCTGACATTAAAATAGTTTGCTTATCTGGATCTAAGTTGTTGTCTATTAACCACTGCTTTTGATTAATAGGCGTTTCAAATTTGTTATCAATAGGAATACCTGTCACTTTAACTGTTGAAGGATCAATACCTACGTCTATGAAGTCTTGTTTCGTTTCTTT  >  USA300TCH1516_ALE/1021397‑1021669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: