| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,216,836 | A→G | 6.5% | K7E (AAA→GAA) | pyrE → | Orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,216,836 | 0 | A | G | 6.5% | 262.8 / 5.6 | 90 | K7E (AAA→GAA) | pyrE | Orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (43/40); new base G (3/3); total (46/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
ATTACGACACCGGAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGGCGCAATCCGCGAT > USA300TCH1516_ALE/1216696‑1216973 | aTTACGACACCGGAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCaa > 1:955520/1‑141 (MQ=255)aTTACGACACCGGAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCaa > 1:947258/1‑141 (MQ=255) cGACACCGCAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATc < 1:499390/141‑1 (MQ=255) gACACCGGAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCa < 1:857946/141‑1 (MQ=255) cGGAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAATTTGC‑TTAGTATAATGGCTAAAGAAATTGCAAAATCattatt > 2:119332/1‑141 (MQ=255) ggAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCAttatta > 1:123510/1‑141 (MQ=255) agaagCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGat < 1:269241/141‑1 (MQ=255) aagCAAGGCAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGatat < 1:267269/141‑1 (MQ=255) gCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATAttt > 2:547364/1‑140 (MQ=255) taataCAGCTTGGTTCGATGGATATTGTAGTCGGTATATCGATTACACAAAGTGACAATTCAGGCGAAAGTTATCATAAAATTAAATAAATTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGa < 2:434640/137‑1 (MQ=255) cAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGa < 1:850836/141‑1 (MQ=255) aaaGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGa < 2:348483/140‑1 (MQ=255) aaaGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGGTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGa < 2:512970/140‑1 (MQ=255) gACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGAATATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGc < 1:441869/141‑1 (MQ=255) gCTTGGTTCGACGCATAGTGTAGTCGGTAGACCGAGTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTa < 1:129242/141‑1 (MQ=255) cTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAGAGAAATTGCAAAATCATTATTAGATATTGCAGCTGTaa > 1:661789/1‑141 (MQ=255) cTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTaa > 1:215689/1‑141 (MQ=255) cTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTaa > 1:745359/1‑141 (MQ=255) cTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTaa < 1:502080/141‑1 (MQ=255) tGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAGAATCATTATTAGATATTGAAGCTGTAACa > 1:360408/1‑141 (MQ=255) tGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAGAATCATTATTAGATATTGAAGCTGTAACa > 1:499536/1‑141 (MQ=255) tGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGAAGAATCATTATTAGATATTGAAGCTGTAACa > 1:964283/1‑141 (MQ=255) gTTCGACGCATATTGTAGTCGGTAGACCGATTCCACAAAGTGACAATACAGTCGAAAGTTATCATAAAATTAACGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGCAACTGTAACAtt > 2:913592/1‑141 (MQ=255) gTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACAtt > 2:54597/1‑141 (MQ=255) gTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACAtt > 1:427372/1‑141 (MQ=255) gTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACAtt > 1:223683/1‑141 (MQ=255) aagCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCAc < 1:269312/139‑1 (MQ=255) cGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATACAGTCAATAGATATCAGAAAAAAAAAG‑AAGTTGGTTTAGTATAATGGCTAAAAAAATTGTATTATCAGGATTATATATTGAAGCTGTAACATTATCAcc < 1:648951/141‑1 (MQ=255) atatTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCaaa < 1:768158/141‑1 (MQ=255) atatTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCaaa < 1:287495/141‑1 (MQ=255) atatTGTAGGCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCaaa < 1:105555/141‑1 (MQ=255) tatTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAAt < 1:53495/141‑1 (MQ=255) tGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGAt > 1:902846/1‑141 (MQ=255) tGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGAt > 1:796431/1‑141 (MQ=255) tGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGAt < 2:947258/141‑1 (MQ=255) tGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGAt < 2:955520/141‑1 (MQ=255) tAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGAttt < 2:275200/141‑1 (MQ=255) aGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACAt > 2:575585/1‑141 (MQ=255) aGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACAt < 1:545065/141‑1 (MQ=255) gATTACACAAAGTGGCAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGt < 1:11237/141‑1 (MQ=255) gATTACACAAAGTGACAATCCAGTCGAAAGGTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGt < 2:532763/141‑1 (MQ=255) aTTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTGTCACCAAATGATTTATATACATGGAGtt > 1:46249/1‑141 (MQ=255) aTTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGtt > 1:73708/1‑141 (MQ=255) acacAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAg < 2:597469/141‑1 (MQ=255) cacaAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAgg > 1:797536/1‑141 (MQ=255) aaGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTAtt > 2:789765/1‑141 (MQ=255) aCAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGAAGTTGAGCTATTAAATc > 2:543185/1‑141 (MQ=255) aCAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGATGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATc > 2:232688/1‑141 (MQ=255) aTCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCAcc < 1:551943/141‑1 (MQ=255) aTCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCAcc > 1:893706/1‑141 (MQ=255) aTCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCAcc > 2:789031/1‑141 (MQ=255) aTCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCAcc > 2:645152/1‑141 (MQ=255) tCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCg < 2:223683/141‑1 (MQ=255) cAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGAt > 1:871675/1‑141 (MQ=255) cGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTAc < 1:821504/141‑1 (MQ=255) aaGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACtgt > 1:36121/1‑141 (MQ=255) aaGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACtgt > 1:824754/1‑141 (MQ=255) aaGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACtgt > 2:521420/1‑141 (MQ=255) aaGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGCAGTTCAGGTATTAAATCACCGATTTACtat > 2:427521/1‑139 (MQ=255) aaaTTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCgtgt < 1:622787/141‑1 (MQ=255) aaaTTAAAGAAAGTTGG‑TTAGTAAAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCgtgt < 1:998832/141‑1 (MQ=255) aaTTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGtt < 1:575585/141‑1 (MQ=255) tAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATGGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACg < 1:54689/141‑1 (MQ=255) tAAAGAAAGTTGG‑TTAGTAGAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACg < 1:717387/141‑1 (MQ=255) aagaaaatTTGT‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGt < 2:832836/137‑1 (MQ=255) aaagaaagTTGG‑TTAGTATAATGGCTAAAGAAATTGCAATATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGt < 2:215689/141‑1 (MQ=255) aagaaagTTGG‑TTAGTATAATGGCTCAAGAAATAGCAAAATATTAATTAGATATTGAAGCTGTAACATTATCACCAAATGCTCTACATACATAGAGTTAAGGTATTAAATCACCAATTTACTGTAATAACAGTGTTACGtt > 2:417431/1‑141 (MQ=255) aagaaagTTGG‑TTAGTATAATGGCTAAAGAAATTGGAAAATCATTATTATATATTGAAGCTGTAACATTATCACCATATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGtt < 2:312321/141‑1 (MQ=255) aagaaagTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTATGtt > 1:161856/1‑141 (MQ=255) aagaaagTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGtt > 1:100827/1‑141 (MQ=255) aagaaagTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGtt > 2:754381/1‑141 (MQ=255) aagaaagTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGtt > 2:651620/1‑141 (MQ=255) aagaaagTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGATGTCAAATTATCACCAAATGAGATATAGGTGGTGAGTTCAGGTATTAAATCACCGATTTACTGTAATAACCGTGTTACGtt > 2:221068/1‑141 (MQ=255) aaaaaagTTGG‑TTAGTATGATGGGTAAAGGAGTTTCAAAATCATGATTAGATAGTGAAGCTGTAACATTATCACTAAATGATTTATATGCATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGtt < 2:941034/138‑1 (MQ=255) agaaagTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTa > 1:10964/1‑141 (MQ=255) gTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCGACGATGTAGTGTGATAACGGTGTGACGTTTggctg > 1:114070/1‑138 (MQ=255) ttAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATTCtt > 1:692768/1‑141 (MQ=255) ttAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCtt < 2:702811/141‑1 (MQ=255) aGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTa < 1:630254/141‑1 (MQ=255) actatataaaGTGCAAAATGATTATTAGATATTGAAGCTGTAACATTATCACAAAATTATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGgc < 2:969216/134‑1 (MQ=255) tAAAGAAATTTCAGAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCATGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGgcgc < 2:964283/141‑1 (MQ=255) tAAAGAAATTGCAGAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGgcgc < 2:360408/141‑1 (MQ=255) tAAAGAAATTGCAGAATCATTATTAGAGAGAGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGgcgc < 2:499536/141‑1 (MQ=255) aGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGGCGCAAt > 1:276675/1‑141 (MQ=255) gAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTTGTTTATCCTTTAGTTCGAGGCGCAATc > 1:333859/1‑141 (MQ=255) aaaTTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGGCGCCATcc > 1:941805/1‑141 (MQ=255) aTTGCAACATCAGTATTAGATATTGAAGCTGTAACATTATCACCAAGTGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCATTTAGTTCGAGTCGCACTCcgc > 2:715506/1‑141 (MQ=255) aTTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGTCGCAATCcgc > 2:460111/1‑141 (MQ=255) ttGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGGCGCAATCcgcg > 1:823160/1‑141 (MQ=255) ttGCAAAATCATTATTAGATATTGAAGCGGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGGCGCAATCcgcg < 2:919844/141‑1 (MQ=255) tcAAAATAATTATTAGATATTGAAGCTGTAACATTAGCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATACCCGTGTTACGTTAGGTTATCCTTTAGTTCGAGGCGCAATCCGCGAt < 1:148331/140‑1 (MQ=255) gCAAAATCGTTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTTAGGTATTAAATCACCGATTTACTGTGATAACATTGTTACGTTAGGTTATCCTATAGTTCGAGGCGCAATCCGCGAt > 2:381415/1‑141 (MQ=255) | ATTACGACACCGGAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGG‑TTAGTATAATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACGTTAGGTTATCCTTTAGTTCGAGGCGCAATCCGCGAT > USA300TCH1516_ALE/1216696‑1216973 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |