Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,416,4580AT100.0% 190.0 / NA 61I216N (ATT→AAT) oppD_4Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/39);  total (22/39)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGT  >  USA300TCH1516_ALE/1416324‑1416592
                                                                                                                                      |                                                                                                                                      
tAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACt                                                                                                                                  <  2:675160/141‑1 (MQ=255)
tAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACt                                                                                                                                  <  2:675074/141‑1 (MQ=255)
 aaCTCAATCATAGTAATCCCTCTTTAAGTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                 <  2:102748/141‑1 (MQ=255)
   cTCAATCATCGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGCTGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTCACCTTCTTTTAAAACATAATTCTACTgcc                                                                                                                               >  2:3628/1‑139 (MQ=255)
     cAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACaa                                                                                                                             >  2:671145/1‑141 (MQ=255)
      aaTCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTTTACTAACatt                                                                                                                            >  2:546007/1‑139 (MQ=255)
      aaTCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                                                            >  1:122365/1‑141 (MQ=255)
        tCATAGTAATCTCTCTTTAATTGTGTTCTATATTTAATTAGACGTGTAGTAAACATACTCAAATGATCATCATTGAAATGATTAATATTAACTCGTTCTGTAATTTGACCTTCTTTTAAAACATAATTGTACTGACAatat                                                                                                                          <  2:678108/141‑1 (MQ=255)
                   ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATg                                                                                                               <  1:454635/141‑1 (MQ=255)
                   ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAAAATAATTGTACTAACAATATTTCAATACATg                                                                                                               >  2:351666/1‑141 (MQ=255)
                   ccTCTTGAAATGTGTTCTATATAAAATTTGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTTACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATg                                                                                                               <  2:725232/141‑1 (MQ=255)
                    ctctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAGACATAATTGTACTGACAATATTTCAATACATGa                                                                                                              >  1:967213/1‑141 (MQ=255)
                     tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAc                                                                                                             <  1:719928/141‑1 (MQ=255)
                         tAATTGTGTTCTATATTTAATTTGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTaa                                                                                                         <  2:867207/141‑1 (MQ=255)
                         tAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTaa                                                                                                         <  1:52823/141‑1 (MQ=255)
                         tAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTaa                                                                                                         <  1:683715/141‑1 (MQ=255)
                          aaTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATTATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAg                                                                                                        >  2:763085/1‑141 (MQ=255)
                            ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                      <  1:893632/141‑1 (MQ=255)
                               ttttCTATATTTAATTAGACGTTCAGTATACAGATGCAAATGCTCATACTTGAAATGATTATTCACCGCTCCTTCAATAATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtg                                                                                                   <  1:485198/139‑1 (MQ=255)
                                gtTCTATATTTAATTAGCCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgt                                                                                                  >  1:543222/1‑141 (MQ=255)
                                gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgt                                                                                                  >  1:216137/1‑141 (MQ=255)
                                       atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACGGACGATGTTTGAATACATGACTTACGTTATGTGTGataa                                                                                           >  1:530528/1‑141 (MQ=255)
                                        tttAACTAGACGTTCAGTATACGGACGCAAATTCTCATACTTGAAATGATTAACATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataat                                                                                          <  1:875508/141‑1 (MQ=255)
                                          tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGTCTTCAGTTATGTGTGCtaataa                                                                                        >  1:556392/1‑141 (MQ=255)
                                          tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                        >  1:16329/1‑141 (MQ=255)
                                             ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaata                                                                                     >  1:449611/1‑141 (MQ=255)
                                               aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATCGTACTGACAATATTTCACTGTGTGAGTTAAGTTATGTGTGATAATAactaat                                                                                   >  1:190365/1‑141 (MQ=255)
                                                taCGTTCAGTATACGGATGCAAATTCTCATATTTTAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACCAAAGTTTAGTACTTGACTTAAGTTATGTGTGATAATAAATAATg                                                                                  <  2:470649/140‑1 (MQ=255)
                                                aacctttaGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATg                                                                                  <  1:458633/134‑1 (MQ=255)
                                                    ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAACCATACTTTTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                              <  2:420992/141‑1 (MQ=255)
                                                    ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                              <  1:808369/141‑1 (MQ=255)
                                                     ttaGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                             <  1:570086/139‑1 (MQ=255)
                                                     tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                             <  1:328715/141‑1 (MQ=255)
                                                         tataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                         >  1:924150/1‑141 (MQ=255)
                                                             cGGATGCAACTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACAACAGTTATGTGCCATAATAAATAATGATCGACCATGTTc                                                                     >  2:183369/1‑141 (MQ=255)
                                                              ggATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCt                                                                    <  2:30545/141‑1 (MQ=255)
                                                                 tGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCGATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAAt                                                                 <  1:836251/141‑1 (MQ=255)
                                                                 tGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAAt                                                                 <  1:972472/141‑1 (MQ=255)
                                                                  gCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATa                                                                >  1:293076/1‑141 (MQ=255)
                                                                   aaaaTGTTCATTCGTGAAATGATTAATATTACCTCGTTCTATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATCCATGCCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                                                               <  2:16499/140‑1 (MQ=255)
                                                                      aTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAat                                                            <  2:535569/141‑1 (MQ=255)
                                                                               cTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaa                                                   <  1:36437/141‑1 (MQ=255)
                                                                                       gATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCa                                           <  1:782390/141‑1 (MQ=255)
                                                                                       gATTAATATTACCTCGTTCAATGATTTGACCTTCTTGTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTGATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCa                                           <  1:3628/141‑1 (MQ=255)
                                                                                          tAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATAAATAACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCa                                        <  2:892293/141‑1 (MQ=255)
                                                                                           aaTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCat                                       >  2:433254/1‑140 (MQ=255)
                                                                                                  ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTa                                <  1:481699/141‑1 (MQ=255)
                                                                                                     cGTTCAATGATTTGAACTTATTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCg                             <  1:911635/141‑1 (MQ=255)
                                                                                                       ttCAATTATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                           <  2:49115/141‑1 (MQ=255)
                                                                                                          aaTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaa                        >  1:258221/1‑141 (MQ=255)
                                                                                                          aaTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGCGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaa                        >  1:258230/1‑141 (MQ=255)
                                                                                                             gATTTGTCCTTCTTTTTAAACAGAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTTACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGc                     <  2:556402/141‑1 (MQ=255)
                                                                                                              aTTTGACCTTCTTATAAAACATGATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCa                    <  2:882143/141‑1 (MQ=255)
                                                                                                                ttGACCGTCTTGTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATc                  <  2:88483/141‑1 (MQ=255)
                                                                                                                      ttcttTTCAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatga            <  1:252161/141‑1 (MQ=255)
                                                                                                                      ttcttTTAAAACAGAATTGTACTGACAATATTACAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatga            <  1:49862/141‑1 (MQ=255)
                                                                                                                       tcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatgat           <  1:763085/141‑1 (MQ=255)
                                                                                                                            tAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAAc      >  2:520147/1‑141 (MQ=255)
                                                                                                                            tAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAAc      >  2:295654/1‑141 (MQ=255)
                                                                                                                              aaaCATAATTGTATTTATAATATTTCTATACATGAATTAATTTATGTGTGATAATAAATAATGTTTGACCATGTACTAATACCATATGCTTTAATAAATCCATCACTTGATTACTGTTCATAGCATCCAATGATGCAACTg    <  2:815461/141‑1 (MQ=255)
                                                                                                                                aCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGt  <  1:163699/141‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGT  >  USA300TCH1516_ALE/1416324‑1416592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: