Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,473,325 C→T 5.0% A9567T (GCA→ACA)  ebh_1 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,473,3250CT 5.0% 254.6 / 2.6 98A9567T (GCA→ACA) ebh_1Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base C (33/54);  new base T (3/2);  total (39/59)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

AACGTCTTTATTGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCTTGTAGTCGTT  >  USA300TCH1516_ALE/1473185‑1473459
                                                                                                                                            |                                                                                                                                        
aaCGTCTTTATTGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTGGTTCAATCGCGTGCTTGCGTGGTGAGTATCTTTGAGCTCCAGGCTGACTGCCTTGTGCTTTATTAATGTTGTTGATGATGCTGTTTGc                                                                                                                                          <  1:489791/141‑1 (MQ=255)
    tCTTTATTGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTACTGTTGTTGATGATGCTGTTTGCCAAt                                                                                                                                      >  2:1003819/1‑141 (MQ=255)
    tCTTTATTGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTAATAAATCGCGTAGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGATGTTGATGATGCTGTTTGCCAAt                                                                                                                                      >  2:666020/1‑141 (MQ=255)
     ttttATTTGCATTAATTTTTGCTTTTGGTATTTCATTGTGATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAATTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAAtt                                                                                                                                     <  2:52289/140‑1 (MQ=255)
     cTTTATTGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCTCTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAAtt                                                                                                                                     <  1:379249/141‑1 (MQ=255)
      tttATTGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAAttc                                                                                                                                    <  1:828583/141‑1 (MQ=255)
       ttATTGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAAttct                                                                                                                                   <  1:949339/141‑1 (MQ=255)
           tGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTTATGATGTTGTTTTGCTATTCT‑TCTg                                                                                                                              >  1:348534/1‑141 (MQ=255)
            ggCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGc                                                                                                                             >  1:330968/1‑141 (MQ=255)
             ttattttttttttCTTTTTTTATTTCATTGGAATGCAACTGTCCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACCGCCTCATTTCTTGTTGCTTTATTTATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCt                                                                                                                            <  1:343402/134‑1 (MQ=255)
             gCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCt                                                                                                                            <  1:159984/141‑1 (MQ=255)
             gCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCt                                                                                                                            <  1:864083/141‑1 (MQ=255)
             gCATCAATTTTTGCTTGTGGTCTTTCATTGGCATTCACTTGTTCAATCGCGTGGTTGCCTGCTGGTTGAACTTGAGATTTAGTCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCt                                                                                                                            <  2:560277/141‑1 (MQ=255)
                 cactttttGCTTTTGGTAGTTCATTGGCATGTACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTAt                                                                                                                        <  1:855202/138‑1 (MQ=255)
                 cAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTAt                                                                                                                        >  1:883185/1‑141 (MQ=255)
                   aTTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTTTTTGCACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTGT‑TCTGCTTTAttt                                                                                                                      >  1:58218/1‑141 (MQ=255)
                      tttGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCTTGATTTCTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTTT‑TCTGCTTTATTTTTc                                                                                                                   <  2:285228/141‑1 (MQ=255)
                        tGCTTTTTGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTTTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTTCTTTATTTTTctc                                                                                                                 >  2:232982/1‑139 (MQ=255)
                         gCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTGTTTTTCGCa                                                                                                                >  1:355226/1‑141 (MQ=255)
                             ttGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATaa                                                                                                            >  1:984225/1‑141 (MQ=255)
                                 tATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGTCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCtt                                                                                                        >  1:297880/1‑141 (MQ=255)
                                 tATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGTCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCtt                                                                                                        >  1:806262/1‑141 (MQ=255)
                                    ttCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTTATTACTTGTTGCTTTATTACTGTTGTTGATGATGCTGTTTGCCACTTCT‑TCTGCTTTATTTTTCGCACTCATCTTGTc                                                                                                     >  2:262415/1‑141 (MQ=255)
                                    ttCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTc                                                                                                     >  2:562467/1‑141 (MQ=255)
                                     tCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAGCTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCt                                                                                                    <  1:841416/141‑1 (MQ=255)
                                     tCATTGGCATGCACTTGTTCAATCGAGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCt                                                                                                    <  2:818425/141‑1 (MQ=255)
                                          ggCATGCAGTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAAGTTGAGATACAGTCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATc                                                                                               <  2:116643/141‑1 (MQ=255)
                                            cATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGGTGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAATCTTGTCTTGATcct                                                                                             >  2:188651/1‑140 (MQ=255)
                                               gCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCg                                                                                          <  1:690334/141‑1 (MQ=255)
                                                 aCTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGGTGTTTGCCAACTCT‑TCTGCTTTATTGTTGCGATTAAGTGTGTCTTGATctggctct                                                                                        >  2:965300/1‑134 (MQ=255)
                                                    tGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTACTTTATTTTTCGCAATAAGCTTATCTTTATCCGTCGCAttt                                                                                     >  2:560703/1‑141 (MQ=255)
                                                     gTTCAATCACGTGGTTGCCTGCTGTTTGAACTTGAGACACAGCCTGATTACTTGTTGCTGTATTAATGTTGTTCATGATGCTGTTTGCCCATTAT‑TATACTTCATTTTTCGACATAACCTTGTCTTGATCCATCGCCttat                                                                                    >  2:263444/1‑139 (MQ=255)
                                                      ttgagttgcgTGGTTGCCTGTTGTTTGAATTTGAGATACAGTGTGATTACTNGTTGCTTTATTAATGTTGTTGATGATGGTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGa                                                                                   <  2:260537/134‑1 (MQ=255)
                                                          aTCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCt                                                                              >  1:809100/1‑141 (MQ=255)
                                                            cgcgTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGTCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTc                                                                            <  2:297880/141‑1 (MQ=255)
                                                            cgcgTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGTCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTc                                                                            <  2:806262/141‑1 (MQ=255)
                                                            cgcgTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTc                                                                            >  2:102670/1‑141 (MQ=255)
                                                            cgcgTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCAGTCGCATTTGA‑AGCTTc                                                                            >  2:639339/1‑141 (MQ=255)
                                                               gTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCACTTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGAt                                                                         <  2:778092/141‑1 (MQ=255)
                                                                tGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGAtt                                                                        <  2:440888/141‑1 (MQ=255)
                                                                 ggTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGAttt                                                                       <  1:828950/141‑1 (MQ=255)
                                                                  gTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTTATCCGTCGCATTTGA‑AGCTTCGATTTc                                                                      <  1:940191/141‑1 (MQ=255)
                                                                  gTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTc                                                                      <  1:14028/141‑1 (MQ=255)
                                                                   ttGCCTGCTGTTTGAATTTGGGATATAGCCTGATTACTTGTTGCTTTATTAATTTTGTTGATGATGCTGTTTCGCTATTCT‑GCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCt                                                                     <  2:567987/141‑1 (MQ=255)
                                                                   ttGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCt                                                                     >  1:75775/1‑141 (MQ=255)
                                                                   ttGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCt                                                                     >  1:112975/1‑141 (MQ=255)
                                                                    tgcttgctGTTTGAACTTGAGATACAGCCTGATTTCTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCtt                                                                    <  2:440184/141‑1 (MQ=255)
                                                                      ccTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCtttt                                                                  <  1:308836/141‑1 (MQ=255)
                                                                      ccTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATCCT‑TCTGCTTTATTTTTCGCAATAAGCCTGTCTTGATCCGTCACATTTGA‑AGCTTCTATTTCtttt                                                                  >  2:389699/1‑141 (MQ=255)
                                                                         gctgTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCACTTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGc                                                               <  2:314432/141‑1 (MQ=255)
                                                                                gAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAg                                                        <  1:693981/141‑1 (MQ=255)
                                                                                gAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGACAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑CGCTTCGATTTCTTTTAGCTTATTAg                                                        >  1:62980/1‑141 (MQ=255)
                                                                                 aactctagaTACAGCCTGGTGAGTTGTTGCTGTAGTAATTTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTGATTTGTCTCAATAAGCTTGTCTGGATACGTCGCATTGGA‑AGCTTCGATTTCTTTTAGCTTATTAGc                                                       <  2:320256/135‑1 (MQ=255)
                                                                                 aaCTTGAGATACAGGCTGATTACTTGTTGCGTCATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGc                                                       <  1:697482/141‑1 (MQ=255)
                                                                                  aCTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGTCTATTGT‑TCTGCTTTATTTTTCGCAATAAGCTTGTATTGATCCGTTTCATTTGA‑AGCTTCGTGTTCTTTTAGCtttttttct                                                      >  2:173728/1‑137 (MQ=255)
                                                                                  aCTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCt                                                      >  1:412963/1‑141 (MQ=255)
                                                                                  aCTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCt                                                      >  1:853302/1‑141 (MQ=255)
                                                                                  aCTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCt                                                      >  1:539786/1‑141 (MQ=255)
                                                                                   cTTGAGATACAGCCTGATTACTTGTTGCTTTATTTATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTa                                                     <  1:627035/141‑1 (MQ=255)
                                                                                   cTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTa                                                     <  1:32635/141‑1 (MQ=255)
                                                                                   cTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTa                                                     <  1:548390/141‑1 (MQ=255)
                                                                                    ttGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTG‑AAATGGTAACTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTaa                                                    <  1:484598/141‑1 (MQ=255)
                                                                                       agaTACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTCTAATCGTCTTTAATCTTGTCTTTACCCCCCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGc                                                 <  1:489007/141‑1 (MQ=255)
                                                                                           tcAGTCTGATTACTTGTGTTTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGa                                             <  2:303068/140‑1 (MQ=255)
                                                                                            cAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGAt                                            <  1:647324/141‑1 (MQ=255)
                                                                                                ttGATTACTTGTTTCTTTATTAATGTTGTTGATGATGTTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCTATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAAt                                        <  1:553154/140‑1 (MQ=255)
                                                                                                cTGATTACTTGTTGCTTTTTTATTGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑TGCTTCGATTTCTTTTAGCTTATTAGCTATAGCTTGATTAAt                                        <  2:91170/141‑1 (MQ=255)
                                                                                                 tGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATa                                       <  2:307565/141‑1 (MQ=255)
                                                                                                   aTTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGa                                     <  2:437876/141‑1 (MQ=255)
                                                                                                     tACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGAtt                                   >  2:785158/1‑141 (MQ=255)
                                                                                                      cgttgttgTTTTATGAGTGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTg                                  <  2:986517/139‑1 (MQ=255)
                                                                                                      aCTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTACTAGATTg                                  >  1:963196/1‑141 (MQ=255)
                                                                                                      aCTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTg                                  >  1:174988/1‑141 (MQ=255)
                                                                                                      aCTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTg                                  <  1:633459/141‑1 (MQ=255)
                                                                                                        ttgttgCTTTAGTAATGTTGTTGATGAAGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAAGAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGaa                                <  2:724559/141‑1 (MQ=255)
                                                                                                           ttgCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATATATTGTAttt                             >  2:185823/1‑140 (MQ=255)
                                                                                                            tgCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGc                            <  1:501076/141‑1 (MQ=255)
                                                                                                            tgCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGc                            <  1:697832/141‑1 (MQ=255)
                                                                                                             tcttggttaATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGcc                           <  2:352605/135‑1 (MQ=255)
                                                                                                                 tattaATGTTGTTGATGATGCTGTTTGGCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑CGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGCTTAATAGTTTGACTTGCCTTGt                       >  1:30607/1‑141 (MQ=255)
                                                                                                                  tttaATGTTGGTGATGATGCTGTTTGCCAATGTT‑TCTTCTTTATTTTTCGCAATAAGTTTGTTTTGATCCGTCGCATTTGA‑AGTTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTc                      <  2:106101/140‑1 (MQ=255)
                                                                                                                  attgATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATATGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTc                      <  2:177362/141‑1 (MQ=255)
                                                                                                                   ttaATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCt                     >  2:179773/1‑141 (MQ=255)
                                                                                                                    taATGTTGTTGATGATGCTGTTTGCAAATTCT‑TCTGTTTTATTTTTCGTAATAAGCTTGTTTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGGTTATTAGTTAAAGCTTGATTAATAGATTGAATTGCCTTGTCtt                    <  2:71823/141‑1 (MQ=255)
                                                                                                                        gttgttGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCACATCCCCAAATCG‑TATGTGTTTGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGc                >  1:238957/1‑141 (MQ=255)
                                                                                                                         ttgttgATTATGCTGTTTGGCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCa               <  1:623902/141‑1 (MQ=255)
                                                                                                                         ttgttgATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTGGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGATTTGCCTTGCCtttgtct               >  1:744632/1‑137 (MQ=255)
                                                                                                                         ttgttgATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAACGCTTGATTAATAGATTTAATTGCCTTGTCTTTAGCa               >  2:472734/1‑141 (MQ=255)
                                                                                                                         ttgttgATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTCAATTGCCTTGTCTTTAGCa               <  2:1011376/141‑1 (MQ=255)
                                                                                                                         ttgttgATGATGATGTTTGCCAATTCT‑TCTGCTATATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCa               <  2:107395/141‑1 (MQ=255)
                                                                                                                            ttgATGATGCTGTTTGCCAATTCT‑TCTGCTGTATTTTTCGCAATAAGCTAGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCt            <  1:232982/141‑1 (MQ=255)
                                                                                                                             tgatgaTGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCtt           <  1:663283/141‑1 (MQ=255)
                                                                                                                              gatgatGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCTTg          >  1:367428/1‑141 (MQ=255)
                                                                                                                              gatgatGCTGTTTGCCAATTCG‑TCTGTTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTATCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTCGCATTTTg          <  2:741227/141‑1 (MQ=255)
                                                                                                                                   tgCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCTTGTAGTc     <  1:165031/141‑1 (MQ=255)
                                                                                                                                   tgCTCTTTGCCAATTCT‑TCTGCTTTCTTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCTTGTAGTc     <  1:16196/141‑1 (MQ=255)
                                                                                                                                    gCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCTTGTAGTct    >  2:144745/1‑140 (MQ=255)
                                                                                                                                     cTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCTTGTAGTCGt   <  1:785158/141‑1 (MQ=255)
                                                                                                                                      tGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCTTGTAGTCGtt  <  1:715406/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
AACGTCTTTATTGGCATCAATTTTTGCTTTTGGTATTTCATTGGCATGCACTTGTTCAATCGCGTGGTTGCCTGCTGTTTGAACTTGAGATACAGCCTGATTACTTGTTGCTTTATTAATGTTGTTGATGATGCTGTTTGCCAATTCT‑TCTGCTTTATTTTTCGCAATAAGCTTGTCTTGATCCGTCGCATTTGA‑AGCTTCGATTTCTTTTAGCTTATTAGCTAAAGCTTGATTAATAGATTGAATTGCCTTGTCTTTAGCATCTTGTAGTCGTT  >  USA300TCH1516_ALE/1473185‑1473459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑