Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,615,451 T→A 5.6% V106V (GTA→GTT fur ← Ferric uptake regulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,615,4510TA 5.6% 230.6 / 3.7 82V106V (GTA→GTTfurFerric uptake regulation protein
Reads supporting (aligned to +/- strand):  ref base T (35/41);  new base A (3/2);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATTTACTATCCTTTACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAGTTCAGCTAACAACTCTAACGTTCTG  >  USA300TCH1516_ALE/1615312‑1615589
                                                                                                                                           |                                                                                                                                          
ttagttACTATCCTTGACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTAATCGATTTCATCTa                                                                                                                                           <  2:268589/137‑1 (MQ=255)
cAATTTACTATCCTTTACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTa                                                                                                                                           <  2:838001/141‑1 (MQ=255)
  aTTTACTATCCTTTACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTaca                                                                                                                                         <  2:1008470/141‑1 (MQ=255)
   tttACTATTCTTTACGTTTTGCTTGGCATGTTTCACACACACCATGGAATGTTAAACGATGATCTAATATTTTAATATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTCACCAATCTTCATCGATTTCATCTacac                                                                                                                                        <  2:554927/141‑1 (MQ=255)
    ttACTATCCTTTACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACg                                                                                                                                       <  1:529901/141‑1 (MQ=255)
    ttACTATCCTTTACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTAAATTTACATGTGTTCATCGATTTAATCTAGACg                                                                                                                                       >  1:697161/1‑141 (MQ=255)
    ttACTATCCTTTACCTTTAGCTTGGCACGTTTCACACAAACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTAGATTTTCAACTTCTGGTAACAAATCTTCATCCATTTCATCAACCCg                                                                                                                                       >  2:931278/1‑141 (MQ=255)
       cTATCCTTTACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGAcc                                                                                                                                    <  1:783722/141‑1 (MQ=255)
        tATCCTTTTCCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACca                                                                                                                                   <  1:685210/141‑1 (MQ=255)
          tCCTTTACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACcaca                                                                                                                                 <  1:887589/141‑1 (MQ=255)
             tttacctttaGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTc                                                                                                                              <  2:801002/141‑1 (MQ=255)
             tttacctttaGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGACTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTc                                                                                                                              >  1:588928/1‑141 (MQ=255)
                acctttaGCTTGACACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAAAAAATCTTCATCGATTTCATATACAAAACCACATTAcat                                                                                                                           >  2:1015511/1‑141 (MQ=255)
                 gctttaGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAACTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCcata                                                                                                                          <  2:707345/140‑1 (MQ=255)
                 cctttaGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCcata                                                                                                                          >  2:871542/1‑141 (MQ=255)
                 cctttaGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCcata                                                                                                                          <  1:283521/141‑1 (MQ=255)
                 cctttaGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCcata                                                                                                                          >  1:67998/1‑141 (MQ=255)
                     taGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAGATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACGACATTCcatacata                                                                                                                      >  1:73129/1‑141 (MQ=255)
                      aGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCatacatac                                                                                                                     <  1:899305/141‑1 (MQ=255)
                      aGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCatacatac                                                                                                                     <  2:975/141‑1 (MQ=255)
                      aGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAAATCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTAAACGACCACATTCCatccatac                                                                                                                     >  2:21173/1‑141 (MQ=255)
                        cTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTa                                                                                                                   <  1:954804/141‑1 (MQ=255)
                         ttGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCACACCTACTaa                                                                                                                  >  2:927824/1‑141 (MQ=255)
                         ttGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCAACACGACCACATTCCATACATACTaa                                                                                                                  >  2:878411/1‑141 (MQ=255)
                         ttGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCAACACGACCACATTCCATACATACTaa                                                                                                                  >  2:405658/1‑141 (MQ=255)
                           ggCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAat                                                                                                                >  1:726336/1‑141 (MQ=255)
                           ggCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAat                                                                                                                >  2:353944/1‑141 (MQ=255)
                               cGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTAGTAACAAATCTTCATCAATTTCATCTACACGACCACATTCCATACATACTAAatgatg                                                                                                            >  2:60397/1‑141 (MQ=255)
                               cGTTTCACACACACCATGGAAAGCTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACAAGACCACATTCGATACATACTAAatgatt                                                                                                            >  2:383673/1‑140 (MQ=255)
                                  ttCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACTTTCCATACATACTACATGAtggtg                                                                                                         >  2:300305/1‑141 (MQ=255)
                                  ttCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTATCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGAtggtg                                                                                                         >  2:68213/1‑141 (MQ=255)
                                     acacacacCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACCCGACCCCATTCCATACTTACTAAATCATGGTGaaa                                                                                                      >  2:1012083/1‑141 (MQ=255)
                                      cacacacCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACAAGACCACATTCCATACATACTAAATGATGGTGGaaa                                                                                                     >  2:42517/1‑141 (MQ=255)
                                        cacacCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATg                                                                                                   <  1:344364/141‑1 (MQ=255)
                                             cATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTGATCGATTTCATCTACACGACGACATTCCATACATACTAAATGATGGTGGAGAGGTTTTg                                                                                              >  1:260398/1‑141 (MQ=255)
                                             cATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTCCACGACCACATTCCCTACATACTAAATAATGCTGGAAATGTCTTg                                                                                              >  2:69014/1‑141 (MQ=255)
                                               tGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTgcg                                                                                            <  2:262840/141‑1 (MQ=255)
                                                ggAAAGTTAAACTATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTgcgc                                                                                           <  1:457722/141‑1 (MQ=255)
                                                  aaaGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCct                                                                                         <  1:634785/141‑1 (MQ=255)
                                                     gTTAAACGATGATCTAAACTTTTCGGATTACACAGATTTTCAACTCGATTTTCAACTGCTGGGAAGAGAGCTGTGTCGGGTTCATCTGCATGACCACATGCCATACTTACTAAATGATGGTGCAAATGTTTTTCACcttct                                                                                      >  2:177903/1‑141 (MQ=255)
                                                       tAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCttcttt                                                                                    <  2:142275/141‑1 (MQ=255)
                                                                atCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATc                                                                           <  1:11620/141‑1 (MQ=255)
                                                                  cTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCaa                                                                         <  1:819909/141‑1 (MQ=255)
                                                                     aaaTTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATAATGGTGAAAATGTTTTGCGCCTTCTTTTCTTAAATCaaaaa                                                                      >  2:716445/1‑140 (MQ=255)
                                                                       aTTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGa                                                                    >  2:915120/1‑141 (MQ=255)
                                                                            aaaaTTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGAc                                                               >  1:495764/1‑141 (MQ=255)
                                                                                     cTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCAcc                                                      <  1:443931/141‑1 (MQ=255)
                                                                                     cTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCAcc                                                      <  1:90123/141‑1 (MQ=255)
                                                                                     cTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCCTTTCTTAAATCAAAACGAGCGACGCCATCAcc                                                      <  2:907432/141‑1 (MQ=255)
                                                                                      tCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGTAAATGTTTTGCGCCTTCTTTTCTTAAATCTAAACTAGCGACGCCATCACCa                                                     >  1:994679/1‑141 (MQ=255)
                                                                                      tCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCa                                                     <  1:174274/141‑1 (MQ=255)
                                                                                      tCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCa                                                     <  2:549572/141‑1 (MQ=255)
                                                                                        aTTTTCAACTCGATTTTCAACTTATTGTAGCGAAGCTTCCTCGATTTCATCTACACGACCACATTCTATACACACGAAATTATCGTCTAAATGTTCTTCGCCTTCTGTTCTTAAATCAAAACTAGCGACGCCATCACCaca                                                   >  2:19159/1‑139 (MQ=255)
                                                                                          tttCAACTTGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGt                                                 <  2:847169/141‑1 (MQ=255)
                                                                                           ttCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGtt                                                >  2:285057/1‑141 (MQ=255)
                                                                                            tCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCCCCTTCTTTTCTTAAATCAAAACGAGAGACGCCATCACCACAGttt                                               >  2:691597/1‑140 (MQ=255)
                                                                                               aCTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGCGCGACGCCATCACCAAAGTTAAtt                                            >  1:767703/1‑141 (MQ=255)
                                                                                               aCTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAAtt                                            >  1:556049/1‑141 (MQ=255)
                                                                                               aCTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAAtt                                            >  1:48618/1‑141 (MQ=255)
                                                                                                cgtgATTTTCAACGTCTGGTAACAAATCTTCATCGATTTCATCGACACTACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAAttt                                           <  2:172082/138‑1 (MQ=255)
                                                                                                 tCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTATACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAAtttt                                          <  2:439071/141‑1 (MQ=255)
                                                                                                  cGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTg                                         >  1:987328/1‑141 (MQ=255)
                                                                                                      tttCAACTTCTTGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGa                                     <  2:947903/141‑1 (MQ=255)
                                                                                                      tttCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGa                                     <  2:544032/141‑1 (MQ=255)
                                                                                                      tttCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGa                                     <  1:871542/141‑1 (MQ=255)
                                                                                                       ttCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGAc                                    <  1:600434/141‑1 (MQ=255)
                                                                                                        tCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACa                                   <  2:67998/141‑1 (MQ=255)
                                                                                                         cAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACTACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTTCGCCTTCTTTTATTAAATCAAAACGAGCGATTCCATCCCCCAATCTAATTTTTTCGaaaa                                  >  2:819279/1‑141 (MQ=255)
                                                                                                           aCTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACt                                <  2:495764/141‑1 (MQ=255)
                                                                                                           aCTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTGTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACt                                <  1:115071/141‑1 (MQ=255)
                                                                                                            cgtCTGGTAACAGATCTTCATCGATTTCAGCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACtt                               <  2:806764/139‑1 (MQ=255)
                                                                                                              tCTGGTAACAAATCTTCATCGATTTCATCAACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACtttt                             <  1:878411/141‑1 (MQ=255)
                                                                                                              tCTGGTAACAAATCTTCATCGATTTCATCAACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACtttt                             <  1:405658/141‑1 (MQ=255)
                                                                                                                tGGTACGAAATCCTCATCGATTTCATTTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCACAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAg                           <  2:347663/141‑1 (MQ=255)
                                                                                                                   tAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAGTTc                        <  2:741408/141‑1 (MQ=255)
                                                                                                                      cAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAGTTCAGc                     <  1:425201/141‑1 (MQ=255)
                                                                                                                      aaaaTCTGCATCGATTTCATCTACACGACCACATACCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAGTTCAGc                     <  2:588928/140‑1 (MQ=255)
                                                                                                                         aTCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTATAGTTCAGCTaa                  >  2:680502/1‑141 (MQ=255)
                                                                                                                              cATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAGTTCAGCTAACAAct             >  1:890895/1‑141 (MQ=255)
                                                                                                                              cATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCAACAACTTTTAGTTCAGCTAACAAct             >  2:970241/1‑141 (MQ=255)
                                                                                                                                  gATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAGTTCAGCTAACAACTCTaa         <  2:247370/141‑1 (MQ=255)
                                                                                                                                         tCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAGTTCAGCTAACAACTCTAACGTTCTg  >  1:412743/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CAATTTACTATCCTTTACCTTTAGCTTGGCACGTTTCACACACACCATGGAAAGTTAAACGATGATCTAAAATTTTAAAATTGAACTCATTTTCAACTCGATTTTCAACTTCTGGTAACAAATCTTCATCGATTTCATCTACACGACCACATTCCATACATACTAAATGATGGTGGAAATGTTTTGCGCCTTCTTTTCTTAAATCAAAACGAGCGACGCCATCACCAAAGTTAATTTTGTCGACAACTTTTAGTTCAGCTAACAACTCTAACGTTCTG  >  USA300TCH1516_ALE/1615312‑1615589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: