Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,646,189 G→C 100% A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,646,1890GC100.0% 302.5 / ‑6.5 96A155G (GCA→GGA) ypdFAminopeptidase YpdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (50/43);  minor base A (0/2);  total (51/45)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGG  >  USA300TCH1516_ALE/1646051‑1646326
                                                                                                                                          |                                                                                                                                         
atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                        <  1:683833/141‑1 (MQ=255)
 ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGcctcctc                                                                                                                                        <  2:271700/141‑2 (MQ=255)
     aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGACTCCTTTTac                                                                                                                                    >  2:84126/1‑141 (MQ=255)
      tCACTTGCATCATCTTGTGGTAGTTCTCCTGTATGATCAGATGCTACTATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTaca                                                                                                                                   <  2:975014/141‑1 (MQ=255)
      tCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCAGTTTGCTGTCTAATATTGCCTTTAGTTATTTTTCAGTCATGCCGTCTGTTaca                                                                                                                                   >  1:874257/1‑141 (MQ=255)
              aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                                           <  1:961452/141‑1 (MQ=255)
               acacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTCCCTTTAATTCGTTTTCTGTCATGCCTCCTTTTCCAACCGTTaaa                                                                                                                          >  2:1029160/1‑141 (MQ=255)
                cacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                         >  1:582880/1‑141 (MQ=255)
                    aTGTGGTAATGCTCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatata                                                                                                                     <  2:6804/141‑1 (MQ=255)
                    aTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTACTTTTACAACAGTTAAAatata                                                                                                                     <  1:990342/141‑1 (MQ=255)
                          tAATGCATCTCTATGACCCGATGCTACAAGCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                                                                               <  2:588849/141‑1 (MQ=255)
                          tAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                                                                               >  1:188078/1‑141 (MQ=255)
                          tAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                                                                               <  2:367788/141‑1 (MQ=255)
                          tAATGCACCTCTATGACCAAAAAAAACAAGCGTATCTAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTGTTCAGGCATGCCTCCTTTTACAACAGTTAAAATATATTCaga                                                                                                               >  1:248513/1‑139 (MQ=255)
                          tAATGCAACTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                                                                               <  2:923829/141‑1 (MQ=255)
                            aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATg                                                                                                             <  1:502381/141‑1 (MQ=255)
                             tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCATTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGt                                                                                                            >  1:647024/1‑141 (MQ=255)
                             tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACACCAGTTAAAATATATTCATCTGt                                                                                                            >  1:637631/1‑141 (MQ=255)
                             tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATAGATTCATATGt                                                                                                            >  1:57941/1‑141 (MQ=255)
                               cACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGttt                                                                                                          <  2:849154/141‑1 (MQ=255)
                                  ctctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcat                                                                                                       <  2:390726/141‑1 (MQ=255)
                                    ctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                                     >  1:291970/1‑141 (MQ=255)
                                     tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                    <  1:113564/141‑1 (MQ=255)
                                     tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                    <  1:559151/141‑1 (MQ=255)
                                     tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTCCCATGAAACCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                    >  1:1020198/1‑141 (MQ=255)
                                     tATGACCAGATGCTACAATCGTATCGAATCATGATCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                    <  1:700062/141‑1 (MQ=255)
                                     tATGACCAGATGCTACAATCATATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                    >  2:179963/1‑141 (MQ=255)
                                       tGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaaca                                                                                                  >  1:304009/1‑141 (MQ=255)
                                         aCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTATTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAat                                                                                                >  1:751189/1‑141 (MQ=255)
                                           cAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCCTATGTTTCCTCAACAatat                                                                                              >  2:341623/1‑141 (MQ=255)
                                               tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTatc                                                                                          >  2:354414/1‑139 (MQ=255)
                                               tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgc                                                                                          >  1:499830/1‑141 (MQ=255)
                                                 gtACAATCGTATCTAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTAGTTTCATTCCTCCTCTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                                                                        <  2:629137/140‑1 (MQ=255)
                                                 cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                                                                        <  2:769640/141‑1 (MQ=255)
                                                 cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGGCAGGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                                                                        <  2:173543/141‑1 (MQ=255)
                                                  tACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAACATTTTAAGTTCCTATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                                                                       <  1:748778/141‑1 (MQ=255)
                                                      aTCGTATCGAATGATGGTCCATCTGCTTCTAATTCTAGCATTTTGCTTTCTAATATTGCATTTAATTCTTTTTCAGTCATGCCTCATTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                   <  2:226977/141‑1 (MQ=255)
                                                      aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                   <  1:284866/141‑1 (MQ=255)
                                                         gTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAACTATATTCATATGGTTCATCAACAATATTAGCTGCTTTTTGa                                                                                >  2:323067/1‑141 (MQ=255)
                                                           aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGCAt                                                                              >  2:806008/1‑141 (MQ=255)
                                                           aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGACTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATGAACAACAATAGCCGCTTGTTTAAt                                                                              >  2:110271/1‑141 (MQ=255)
                                                            tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCATTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCCTCCACACTATTAGCTGCTTTTTGAAtt                                                                             >  2:401884/1‑141 (MQ=255)
                                                            tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                             >  1:324882/1‑141 (MQ=255)
                                                            tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGAATTTATTGCTGTTTTAGTAATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAATAATATTAACTACTTTTTGAAtt                                                                             >  2:510527/1‑141 (MQ=255)
                                                            tCGAATCATGGTCCGTCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATATTTCATCAACAATATTAACTGCTTTTTGAAtt                                                                             >  2:129870/1‑141 (MQ=255)
                                                                atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAACATATATTCATCTGTTTCATCAACAATATTAGCTGCTTTTTTAATTaaat                                                                         >  2:379449/1‑140 (MQ=255)
                                                                        ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCg                                                                 >  1:45839/1‑141 (MQ=255)
                                                                         cATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGt                                                                <  2:450553/141‑1 (MQ=255)
                                                                             tGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGc                                                            <  1:294088/141‑1 (MQ=255)
                                                                                  ttAATTTTTGCATTTTGCTTTCTAATATTGCGTTTAATTCTTTTTCAGTTATGCCTCCTTTTACATTAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTAATTAAAGCAATTTCGTCAGCATCtt                                                       <  2:445453/140‑1 (MQ=255)
                                                                                  ctcaTTCTTGCATTTTCCTTTCTATTATTGTATTTAATTATTTTTCAGTCCTGAATACTTTTACAACAGTTAAAATGTATTCATATGTTTCATCACCCATACTAGCTGCTTTTTGAATTAAAGCAATCTCGTCAGCATCtt                                                       <  2:763337/138‑1 (MQ=255)
                                                                                  cTAATTCTAGCATTTTGCTTTCTAATCTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                       <  2:652028/141‑1 (MQ=255)
                                                                                  cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                       <  2:499830/141‑1 (MQ=255)
                                                                                  cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                       <  2:235211/141‑1 (MQ=255)
                                                                                   tatgtCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAAAGTATTCATATGTTTCATAAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                      <  2:124362/137‑1 (MQ=255)
                                                                                      ttCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGAc                                                   <  2:566536/141‑1 (MQ=255)
                                                                                      ttCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTATTTAATTAAAGAAATTACGTCAGCATCTTTGAc                                                   >  1:280485/1‑141 (MQ=255)
                                                                                       tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAACCAATTTCGTCAGCATCTTTGACg                                                  >  1:296342/1‑141 (MQ=255)
                                                                                          aGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                               >  2:483363/1‑141 (MQ=255)
                                                                                          aGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                               >  2:498291/1‑141 (MQ=255)
                                                                                           gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                              >  1:621215/1‑141 (MQ=255)
                                                                                           gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTCTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                              >  1:760621/1‑141 (MQ=255)
                                                                                              ttttGCTGTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTaa                                           <  2:239223/141‑1 (MQ=255)
                                                                                                 tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACATTATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTGTAAttt                                        >  1:39819/1‑141 (MQ=255)
                                                                                                 tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                                        <  2:291970/141‑1 (MQ=255)
                                                                                                 tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                                        >  1:115953/1‑141 (MQ=255)
                                                                                                 tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAACCGTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                                        >  1:287050/1‑141 (MQ=255)
                                                                                                  gCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAATAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTTACCTCTATTAtttt                                       >  2:951138/1‑141 (MQ=255)
                                                                                                  gCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtttt                                       >  1:340284/1‑141 (MQ=255)
                                                                                                  gCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtttt                                       >  2:443426/1‑141 (MQ=255)
                                                                                                   cTTTCTAATATTGCCTTTAATTATTATTAAGTCATACCTCCTTTTACAACATTTAAACTATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAATCAATTTCGTAAGAATCTTTGATGTCTCTAATTTTa                                      >  2:21801/1‑141 (MQ=255)
                                                                                                     ttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATc                                    >  2:1029034/1‑141 (MQ=255)
                                                                                                     ttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAATCAGTGAGAATTTTTGACGTCTCTATTTTTAtt                                    >  2:5824/1‑140 (MQ=255)
                                                                                                      tCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCt                                   <  1:30826/141‑1 (MQ=255)
                                                                                                       ttAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTa                                  <  2:289670/140‑1 (MQ=255)
                                                                                                          atatTGCCTTTAATTCTTTTTCAGTTATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAGAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAg                               <  2:445816/141‑1 (MQ=255)
                                                                                                          atatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAg                               <  2:519797/141‑1 (MQ=255)
                                                                                                           tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGt                              <  2:591362/141‑1 (MQ=255)
                                                                                                               ctcTTTAATTCTTTTGCAGTTATGCCTCCTTTTACAACATTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                          <  2:969114/139‑1 (MQ=255)
                                                                                                                 cTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                        >  1:109707/1‑141 (MQ=255)
                                                                                                                 cTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                        >  1:83974/1‑141 (MQ=255)
                                                                                                                  tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                       <  2:450848/141‑1 (MQ=255)
                                                                                                                  tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                       <  2:670037/141‑1 (MQ=255)
                                                                                                                  tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTAATATGTATTGGCAGCATTATTCTCTTGGTGTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                       <  2:732711/141‑1 (MQ=255)
                                                                                                                      aTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                   >  1:283513/1‑141 (MQ=255)
                                                                                                                        tctttcttAGTCATGGCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTACTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATTTACAGTATTAGAAATGCtt                 <  2:752383/141‑1 (MQ=255)
                                                                                                                        tCTTTTTCAGTCAGGCCTCCTTTTACAACAGGTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt                 <  2:761876/141‑1 (MQ=255)
                                                                                                                         cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTATAGTATTAGAAATTCttt                >  2:535017/1‑140 (MQ=255)
                                                                                                                         cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttt                >  1:97522/1‑140 (MQ=255)
                                                                                                                         cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta                >  1:776287/1‑141 (MQ=255)
                                                                                                                          tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCTTCTTTGTCGTCTCTAATTTTATCTACAGTCTTCGCAATGCttat               >  2:150983/1‑141 (MQ=255)
                                                                                                                          tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat               <  2:580757/141‑1 (MQ=255)
                                                                                                                           ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt              >  1:175949/1‑141 (MQ=255)
                                                                                                                           ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt              >  1:413706/1‑141 (MQ=255)
                                                                                                                                 gTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACACTATTAGAAATGCTTATTAATGat        >  2:483535/1‑141 (MQ=255)
                                                                                                                                  tCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata       <  1:498291/141‑1 (MQ=255)
                                                                                                                                  tCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata       <  1:483363/141‑1 (MQ=255)
                                                                                                                                       cctcctTTTACAACAGTTAAAATATATGCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACgg  <  1:249621/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
ATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGG  >  USA300TCH1516_ALE/1646051‑1646326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: