BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,775,041	A→G	6.5%	D715D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,775,041	0	A	G	6.5%	237.9 / 2.2	105	D715D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (42/51); new base G (4/5); total (49/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTC  >  USA300TCH1516_ALE/2774901‑2775181

cTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGaa                                                                                                                                               >  1:471738/1‑141 (MQ=255)

       aatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTat                                                                                                                                       >  1:615786/1‑141 (MQ=255)

        tcttgattcGCTTTCTGAGTCCTAATTGCTATCTGAATCTTAGTCGCTTTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTatc                                                                                                                                      <  2:102753/138‑1 (MQ=14)

           tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGT‑CGCTgtctga                                                                                                                                   >  2:340535/1‑141 (MQ=11)

           tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGCATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTGTCTGAGTCTGAGT‑CGCTatctga                                                                                                                                   >  2:578229/1‑141 (MQ=255)

               tcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGACTGAAT‑CAGTgtctgatact                                                                                                                               >  2:269942/1‑137 (MQ=11)

                ctctgtctgtGTCTTAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTatctgaatctg                                                                                                                              <  2:118635/139‑1 (MQ=14)

                 gCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTatctgaatctgg                                                                                                                             >  1:499952/1‑140 (MQ=255)

                 gCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTatctgaatctga                                                                                                                             >  1:957954/1‑141 (MQ=255)

                    gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGACTCGCTGTCTGAGTCTGAGTCGATGGCCGAATCTGAAT‑CGCTCTCTGCATCTGAGTc                                                                                                                          >  2:680445/1‑141 (MQ=14)

                     tCTGAGTCCGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCg                                                                                                                         <  1:987498/141‑1 (MQ=255)

                     tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCg                                                                                                                         <  1:870837/141‑1 (MQ=255)

                     tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGACTCGCTGTCTGAGTCTGAGTCGCTGTCTGAACCTGAGT‑CGCTatatgaatccgcctcc                                                                                                                         >  2:45893/1‑134 (MQ=11)

                      cTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTGTCTGAATCTGAGtggg                                                                                                                        >  2:949631/1‑138 (MQ=14)

                        gAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTGTCTGAATCTGAGTCGCTg                                                                                                                      >  1:482065/1‑141 (MQ=255)

                         aGTCCGAATCGCCATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGCGTCCGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgt                                                                                                                     <  1:147326/141‑1 (MQ=255)

                          gTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgtc                                                                                                                    <  1:309508/141‑1 (MQ=255)

                          gTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgtc                                                                                                                    <  1:849618/141‑1 (MQ=255)

                          gTCCGAACCGCAATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgtc                                                                                                                    <  1:734314/141‑1 (MQ=255)

                            ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CTCTATCTGAGTCCGAGTCGCTgtctg                                                                                                                  >  2:428889/1‑141 (MQ=255)

                                aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgtctgagtc                                                                                                              >  1:1011601/1‑141 (MQ=255)

                                 tCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCGATCTGAGTCTGAGTGGCCTTCAAAAGCTTAGTCGCTGTCTGTGTCTGCCT‑CGCTATCTGCATCTGAGTCGCAgtctgagtct                                                                                                             >  1:240170/1‑141 (MQ=14)

                                  tgCTATCTGATTCTGAGTCGGTGTCTTAGTCCCAGTCCGTGTCTGAGTCTTAGTAACTAGCGTAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTGTGAG‑ATGCATGCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                                                                                            <  2:417245/140‑1 (MQ=2)

                                               tgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctga                                                                                               >  1:486477/1‑141 (MQ=255)

                                               tgaGTCGCTGGCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctga                                                                                               <  1:302739/141‑1 (MQ=255)

                                               ggtgTCGCTGTCTTAGTCTGATTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGT‑CGCTATCTGAgtctgagtcgctgtctgagtctgagtcgctgtctga                                                                                               <  2:607562/138‑1 (MQ=14)

                                                  gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                                            >  1:382031/1‑141 (MQ=255)

                                                   tCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGACT‑CGCTGTCTGAATCTGACTCGCCGTCACAGtctgagtcgctctctgaatcc                                                                                           >  2:965513/1‑140 (MQ=14)

                                                   tCGCTCTCCGAGTCTGACTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatct                                                                                           <  2:964021/141‑1 (MQ=14)

                                                   tCGCGGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatct                                                                                           <  2:932950/141‑1 (MQ=255)

                                                    ctcTGTCTTATTTTGAGTTGCTATTTGAATCTGTTTCGCTTTCTGAGTCTGCGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                                                                                          <  2:969830/139‑1 (MQ=14)

                                                     tcTGTCTTAGTCTGATTCTCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTTAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAAGCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                         <  1:468047/140‑1 (MQ=17)

                                                     gCTTTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                         <  1:190857/141‑1 (MQ=255)

                                                     gCTGTCTGAGTCTGATTCGCTCTCTGAGTCTGTGTCGCTCTCTGAGTCTGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                         <  2:272368/141‑1 (MQ=14)

                                                     gCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGCCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                         <  1:375722/141‑1 (MQ=255)

                                                      cTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag                                                                                        >  1:228606/1‑141 (MQ=255)

                                                         tctggtgctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                                     <  1:772598/134‑1 (MQ=255)

                                                         tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGATT‑CGCTATCTGAATCTGagtcgctatctgagtctgagtcgctatctgagtctgcttcg                                                                                     >  2:395618/1‑136 (MQ=14)

                                                            gagtctgaTTCGCTGTCTGAATCTGAGTCGCTATCTGTGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                                                                                  <  2:559465/141‑1 (MQ=255)

                                                                  gaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCAGAAG‑CGCTATCTGAGTCTGAGTCTCTGTCTGAGtctgagtcactatcagagtctgaatcgctgtctgag                                                                            >  2:10170/1‑140 (MQ=11)

                                                                   tgtCGCTATCTGAATCTGAGTCGGTATCTGAGTGTGTGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGT‑CGCTATCTGAGTCTGAGTCGCTATCTGAGtctgagtcgctgtctgaatctgagtcgctttctgagt                                                                           <  2:702856/139‑3 (MQ=11)

                                                                     tCGCTGTCCGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAGT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgagtctgagtctctgtgtgagcgt                                                                         >  2:866673/1‑137 (MQ=11)

                                                                      ctcTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg                                                                        <  1:329076/139‑1 (MQ=255)

                                                                      cGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg                                                                        <  1:4386/141‑1 (MQ=255)

                                                                        ctctctgagtcttaGTCGCTGTCTGAGTCTGAGTCGCTGTCCGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgaatctgaa                                                                      <  2:792451/138‑1 (MQ=14)

                                                                        cTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAatcgctgtctgactctgattcactgtctgtgtctgat                                                                      >  2:868964/1‑140 (MQ=14)

                                                                        cTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGtcgctgtctgaatctgaatcgctgtctgaatctgaa                                                                      >  2:444048/1‑141 (MQ=255)

                                                                             ggagtctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCt                                                                 <  1:314013/137‑1 (MQ=255)

                                                                                atctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTgtc                                                              >  2:346128/1‑141 (MQ=255)

                                                                                 tctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGCGTCGCTGTCTTAATCTGAATCGCTgtct                                                             >  1:946163/1‑141 (MQ=255)

                                                                                 tctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtct                                                             >  1:293392/1‑141 (MQ=255)

                                                                                  ctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctg                                                            >  2:269229/1‑141 (MQ=255)

                                                                                  ctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctg                                                            <  1:860201/141‑1 (MQ=255)

                                                                                  ctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctg                                                            <  1:720082/141‑1 (MQ=255)

                                                                                    gaGTGGCTTTCGCAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTCTCTGAGTCTGAGT‑CGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgag                                                          <  2:426362/141‑1 (MQ=14)

                                                                                    gaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgag                                                          <  1:440511/141‑1 (MQ=255)

                                                                                        cGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtctg                                                      <  1:166563/141‑1 (MQ=255)

                                                                                        cGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtctg                                                      <  1:661790/141‑1 (MQ=255)

                                                                                        cGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtctg                                                      <  1:659055/141‑1 (MQ=255)

                                                                                            aTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                  <  1:534640/141‑1 (MQ=255)

                                                                                                  gactgattcgctgtctgaatctgagtctctgtctgagtCTGAAT‑CGCTATCTGAATATGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatc                                            <  2:393237/139‑1 (MQ=255)

                                                                                                    ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctg                                          <  1:807595/141‑1 (MQ=255)

                                                                                                       ggtcgctgtctgaatctgactcgctgtctgagtctgaAT‑CGCTATCTCAATCTGAGTCTCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTCtctgagtctgaatcgctatctgagt                                       <  2:471738/140‑1 (MQ=14)

                                                                                                        gtcgttatcggagtctggggcgctgtctgaatctgaAT‑CGCTATCTGATTCTGAGTCTCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtc                                      <  2:813772/141‑1 (MQ=14)

                                                                                                        gtcgctgtctgcgtctgaggcgctgtctgagtcggaAT‑CGCTTTCCGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtc                                      <  2:299271/141‑1 (MQ=17)

                                                                                                        gtcgctctctgggcccgattcgctgtccgagtctgaAT‑CGCTATCTGATTCTGAGTCGCTGTCTGAGTCCGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtc                                      <  2:539179/141‑1 (MQ=17)

                                                                                                         tcgctgtctgagtctgagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGTTGTGTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGACTCGCAGtctgagtctgaatcactatctgattct                                     >  2:779878/1‑141 (MQ=14)

                                                                                                         tcgctgtctgagtctgagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCTGAATCGCTGtctgagtctgaatcgatatctgtgttt                                     >  2:396015/1‑139 (MQ=255)

                                                                                                         tcgctgtctgagtctgagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGACTCGCTAtctgagtctgaatcgctatctgggacc                                     >  2:587803/1‑136 (MQ=255)

                                                                                                           gctgtctgagtctgagtcgctgtgtgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctga                                   <  1:279559/141‑1 (MQ=255)

                                                                                                           gctgtctgagtctgagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctga                                   >  1:765963/1‑141 (MQ=255)

                                                                                                               tctgagtctgagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcg                               >  1:111436/1‑141 (MQ=255)

                                                                                                               tctgagtctgagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCCGTCACTCAGtctgagtctgagtcgctatctgagtctgagtcg                               >  1:438145/1‑141 (MQ=14)

                                                                                                               tctgagtctgagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTTtctgcgtctgaatcgcgttctgagtctgaatcg                               >  2:950336/1‑141 (MQ=14)

                                                                                                                 ggagtctgagtcgctgtctgagtctgaGT‑CGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAATCTGAATCGCTatctgaatctgaatcgctatctgaatctgaatcgct                             <  2:382031/140‑1 (MQ=11)

                                                                                                                    gtctgagtcgctgtctgagtctgcAT‑CGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCtgagtctgaatcgctatctgagtctgaatcgctatc                          <  2:901923/141‑1 (MQ=255)

                                                                                                                        gcgtcgctgtctgactctgaGT‑CGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTCTCTGAGTCGGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGtctgaatcgctatctgagtctgaatcgctatctgag                      <  2:868863/139‑1 (MQ=11)

                                                                                                                        gagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGtctgaatcgctatctgagtctgaatcgctatctgag                      >  1:786318/1‑141 (MQ=255)

                                                                                                                        gagtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGtcggaatcgctatctgagtctgaatcgctatctgag                      >  1:809104/1‑141 (MQ=255)

                                                                                                                          gtcgctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCtgaatcgcgatctgagtctgaatcactatctgcgtc                    >  2:735991/1‑141 (MQ=255)

                                                                                                                             gctgtctgagtctgaAT‑CGCTGTCTGTTTCGTTTTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAatcgctatctgagtctgaatcgctatctgagtctga                 <  1:696788/141‑1 (MQ=18)

                                                                                                                              gtgtctgtgtctgtAC‑AGCTATCTGAATGTGAGTCGCTGTCTGAGTCTGGGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                <  1:776582/140‑1 (MQ=17)

                                                                                                                              ctgtctgagtctgaAT‑CGCTATCTGTTTCTAAGTCTCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                <  1:696684/141‑1 (MQ=255)

                                                                                                                              ctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                <  1:957767/141‑1 (MQ=255)

                                                                                                                              ctgtctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                <  1:73902/141‑1 (MQ=255)

                                                                                                                                gtcgtagtctgaAT‑CTCTATCTGGATATATTTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCgctatctgagtctgaatcgctatctgagtctgaatc              <  2:672009/136‑1 (MQ=17)

                                                                                                                                 tctgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGGGTCTGAGTCa             >  1:417172/1‑141 (MQ=255)

                                                                                                                                  ctgcttctgaAT‑CGCTATGTGGCTATTTGTCACCGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCAc            <  1:277265/136‑1 (MQ=17)

                                                                                                                                   tgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGCGTCTGAGTCACt           >  1:153102/1‑141 (MQ=255)

                                                                                                                                   tgagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGACGCTGTCTGACTCTGAATCGCAGTCTGAATCTGAATCGCTGTCTGAATCTGCATCGCTATCTGAGTCTGACTCGGTACTCGAGTCTGACTCaat           >  2:124612/1‑139 (MQ=14)

                                                                                                                                    gagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTg          >  1:723608/1‑141 (MQ=255)

                                                                                                                                    gagtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCCGAATCCCtt          >  2:125912/1‑140 (MQ=255)

                                                                                                                                    gagtctgaAT‑CGCTATCTGAATCTAAGTCGCTGTCTGAGTCTGAGTCGCTGGCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTg          >  1:866573/1‑141 (MQ=255)

                                                                                                                                      gtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTc        <  1:18629/141‑1 (MQ=255)

                                                                                                                                      gtctgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTc        >  1:998801/1‑141 (MQ=255)

                                                                                                                                         tgaAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTACCTGAGTCTGAATCACTGTCTGa     <  1:22913/141‑1 (MQ=255)

                                                                                                                                         ggaAT‑CGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGa     <  2:808680/140‑1 (MQ=255)

                                                                                                                                           aaT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGATTCTGAATCGCTATCTGCTCCTGAATCGCTATCTGATTCTAAATCGCTATCTGAGt   >  2:554011/1‑141 (MQ=17)

                                                                                                                                           aaT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGt   >  1:358588/1‑141 (MQ=255)

                                                                                                                                            aT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTc  >  1:595487/1‑141 (MQ=255)

CTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTC  >  USA300TCH1516_ALE/2774901‑2775181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`