BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000731	81	N→G	intergenic (–/‑151)	– / → repA	–/replication protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	81	0	N	G	97.8%	292.0 / ‑5.5	93	intergenic (–/‑151)	–/repA	–/replication protein A
Reads supporting (aligned to +/- strand): ref base N (0/0); major base G (39/51); minor base C (0/2); total (39/54)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGG  >  CP000731/1‑215

tatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGaaa                                                                                                                                     >  1:290221‑M1/58‑141 (MQ=255)

 atttatcaagtgtttataaccccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAAGATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAAc                                                                                                                                    >  2:283278‑M1/57‑141 (MQ=255)

  tttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACt                                                                                                                                   <  1:158914‑M1/86‑1 (MQ=255)

    tatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGt                                                                                                                                 <  1:63555‑M1/88‑1 (MQ=255)

    tatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATAAAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGt                                                                                                                                 <  1:38787‑M1/88‑1 (MQ=255)

    tatcaagggtttataaacccttgataaagctatattttcccttttataagtatATGTAAAGTGCTATAATAAATTTTATATAACCAAAACCCCAACTACTACTAATACTCGAGGTTTTGAAATACAACATTTACAAACTGt                                                                                                                                 <  2:407510‑M1/88‑1 (MQ=255)

       caagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGttta                                                                                                                              <  1:212321‑M1/91‑1 (MQ=255)

       caagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTGTAGAAACTGttta                                                                                                                              <  1:268663‑M1/91‑1 (MQ=255)

       caagggtttataaacccttgataaagctatattttcacttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGttta                                                                                                                              <  1:41027‑M1/91‑1 (MQ=255)

         atggtttataaactcttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtttatt                                                                                                                            >  2:226203‑M1/49‑141 (MQ=255)

             tttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAA‑CCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatat                                                                                                                       >  1:306920‑M1/45‑141 (MQ=255)

              ttataaacccttgataaagctatattttcccttttataagtttATTTAACCAGCTCATTTGAACTTAATATAACTATATCTCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatat                                                                                                                       >  1:418008‑M1/44‑141 (MQ=255)

              ttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGTATTTCGTAATAAAACTTTTACACACTGTTTATTtatat                                                                                                                       <  2:105036‑M1/98‑1 (MQ=255)

              ttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatat                                                                                                                       >  1:226799‑M1/44‑141 (MQ=255)

               tataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatata                                                                                                                      <  2:355581‑M1/99‑1 (MQ=255)

               tataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatata                                                                                                                      >  1:314237‑M1/43‑141 (MQ=255)

                 taaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAt                                                                                                                    >  2:339387‑M1/41‑141 (MQ=255)

                   aacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                  >  2:390869‑M1/39‑141 (MQ=255)

                         tgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAATCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATa                                                                                                            <  1:430430‑M1/109‑1 (MQ=255)

                           ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                          >  2:226206‑M1/31‑141 (MQ=255)

                            taaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACtt                                                                                                         <  1:138424‑M1/112‑1 (MQ=255)

                             aaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATActtt                                                                                                        <  2:309172‑M1/113‑2 (MQ=255)

                              aagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGt                                                                                                       <  1:7014‑M1/114‑1 (MQ=255)

                                tctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTCCTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtat                                                                                                     >  1:236802‑M1/26‑141 (MQ=255)

                                gctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtat                                                                                                     <  1:15136‑M1/116‑1 (MQ=255)

                                   atattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtattat                                                                                                  >  1:218960‑M1/23‑141 (MQ=255)

                                         tcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTCTATTATATAatt                                                                                            <  2:302448‑M1/125‑2 (MQ=255)

                                            cttttataagtttATTTAAATTGCTATAATAAACTTAATATAACAAAAACTCCAACTACTGGTAATTGTTGAGTTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaat                                                                                         >  2:332325‑M1/14‑141 (MQ=255)

                                               tggtaagttgATTTAAAGTGCTAGAATAAACTGAATATAACAAAAACCCTAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataat                                                                                      >  1:401085‑M1/11‑141 (MQ=255)

                                                tataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAatgaatacta                                                                                     <  2:136099‑M1/132‑4 (MQ=255)

                                                 ataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAatat                                                                                    <  2:182887‑M1/133‑1 (MQ=255)

                                                 ataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAatat                                                                                    <  2:298298‑M1/133‑1 (MQ=255)

                                                  taagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATCATGAATaatatt                                                                                   <  2:57493‑M1/134‑2 (MQ=255)

                                                     gtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTa                                                                                <  1:84192‑M1/137‑1 (MQ=255)

                                                      tttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCAAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGACTAATATCTtat                                                                               <  2:257961‑M1/138‑2 (MQ=255)

                                                       ttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGt                                                                              >  1:186858‑M1/3‑141 (MQ=255)

                                                          tttAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGGTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCa                                                                           >  1:7928/1‑141 (MQ=255)

                                                             aaaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGc                                                                        >  1:283605/1‑141 (MQ=255)

                                                              aaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTCTATTATATAATGAATAATATCTTAATTCAACCa                                                                       >  2:140839/1‑141 (MQ=255)

                                                                gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                     <  2:356496/141‑1 (MQ=255)

                                                                gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                     <  1:424506/141‑1 (MQ=255)

                                                                gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                     <  2:81749/141‑1 (MQ=255)

                                                                   cTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTaa                                                                  >  2:134484/1‑141 (MQ=255)

                                                                   cTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTaa                                                                  <  1:294534/141‑1 (MQ=255)

                                                                    ttaaataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGGTTATTTATATGATATTTGATACTTGTATTATATAATGAATATTATCTTAGTGCTAGCACCTaaa                                                                 >  1:61343/4‑141 (MQ=255)

                                                                    tataATAAATTTAATATAACAAAAACCCCAATTACTGATAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTaaa                                                                 <  2:212900/141‑1 (MQ=255)

                                                                    tataATAAACTTAATATAACAAAAACTCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTTTATTATATAATGAATAATATCTTAGTGCAACCACCTaaa                                                                 >  2:31023/1‑141 (MQ=255)

                                                                    tataATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTaaa                                                                 >  1:200168/1‑141 (MQ=255)

                                                                     ataataAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGAGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAAt                                                                >  1:123085/1‑141 (MQ=255)

                                                                       aataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCa                                                              <  1:112700/141‑1 (MQ=255)

                                                                        ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                             <  2:8841/141‑1 (MQ=255)

                                                                        ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACGGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                             <  2:14498/141‑1 (MQ=255)

                                                                        ataaACTTAAGATAACAAAAACCCCAACTGATGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                             <  2:301295/141‑1 (MQ=255)

                                                                          aaaCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATta                                                           >  2:141672/1‑141 (MQ=255)

                                                                              ttAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaata                                                       <  1:138007/141‑1 (MQ=255)

                                                                                   ataACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGt                                                  <  1:274122/141‑1 (MQ=255)

                                                                                     aaCGAAAATCACAACTACTGATAATATTCGTGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaa                                                <  2:128923/141‑1 (MQ=255)

                                                                                     aaCAAAAACCCCAACTACTGGTAATAGTCGGGGTTAGGTCAGAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaa                                                <  1:342952/141‑1 (MQ=255)

                                                                                      aCAAAAACCCCAACTACTGGTAATAGTCGGGGTCTGGTAATAAAACGTTTATAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaaa                                               <  1:166744/141‑1 (MQ=255)

                                                                                       cAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTACATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCTTTAAATAAATGTAAAg                                              >  2:319485/1‑141 (MQ=255)

                                                                                          aaaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCta                                           <  1:43759/141‑1 (MQ=255)

                                                                                             cgccAACTACGGGTAATAGTCTGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                        <  2:331552/139‑1 (MQ=255)

                                                                                             ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTATTGCAAGTACCTACATAATTAAATAAATGTATAGCtaaaa                                        >  2:325904/1‑140 (MQ=255)

                                                                                             ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                        >  1:201783/1‑141 (MQ=255)

                                                                                             ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                        >  1:241845/1‑141 (MQ=255)

                                                                                             ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTGATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                        >  1:310465/1‑141 (MQ=255)

                                                                                              cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                       <  2:84192/141‑1 (MQ=255)

                                                                                              cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                       >  1:6489/1‑141 (MQ=255)

                                                                                              cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                       >  1:344383/1‑141 (MQ=255)

                                                                                              cccAACAATTCGTAATGGTCGCCGTTTGCTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                       <  1:143094/141‑1 (MQ=255)

                                                                                                   atactGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAAc                                  <  2:116703/140‑1 (MQ=255)

                                                                                                    tactGGTAATAATCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACg                                 <  1:7361/141‑1 (MQ=255)

                                                                                                     actGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                >  2:382259/1‑141 (MQ=255)

                                                                                                     actGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                <  1:98276/141‑1 (MQ=255)

                                                                                                     actGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                <  1:107648/141‑1 (MQ=255)

                                                                                                       tGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGttt                              >  1:217784/1‑141 (MQ=255)

                                                                                                           aaTATTAGGGGTTTAGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTttatt                          <  2:75382/141‑1 (MQ=255)

                                                                                                               gTCGGGTGTTGGTAATAAAACTTGTAGAAACTGTTTATTTATATAATTTTCGATACGTGTATTATATAATGAATAATATCTTAGTGCAAGAACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACct                      <  2:40975/141‑1 (MQ=255)

                                                                                                               gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACct                      <  1:6351/141‑1 (MQ=255)

                                                                                                               gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACct                      <  1:298298/141‑1 (MQ=255)

                                                                                                               gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACct                      <  1:221925/141‑1 (MQ=255)

                                                                                                               gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACct                      >  2:207500/1‑141 (MQ=255)

                                                                                                               gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACct                      <  1:182887/141‑1 (MQ=255)

                                                                                                                  ggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCtt                   >  2:398518/1‑141 (MQ=255)

                                                                                                                    ggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATGATATCTTAGTGAAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAc                 <  2:41027/141‑1 (MQ=255)

                                                                                                                     gTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAcc                <  1:141537/141‑1 (MQ=255)

                                                                                                                        tGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaa             >  1:273007/1‑141 (MQ=255)

                                                                                                                         ggTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAACTACATAAAAACGTTTCATTACCTCTTACCAAAc            >  2:217110/1‑141 (MQ=255)

                                                                                                                               aaaaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAat      <  1:338870/141‑1 (MQ=255)

                                                                                                                                aaaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAata     <  2:268663/141‑1 (MQ=255)

                                                                                                                                 aaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAatat    <  2:233556/141‑1 (MQ=255)

                                                                                                                                   cGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAACCTAACATATgg  >  1:103360/1‑141 (MQ=255)

                                                                                                                                   cGTTTAGAAACTGTTTATTTATATAATTTTCGATACCAGACAGAGTGAAGGAATACTATATGACCACTATCAGTGAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATgg  >  1:104332/1‑141 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGG  >  CP000731/1‑215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`