Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,416,4580AT100.0% 67.8 / NA 24I216N (ATT→AAT) oppD_4Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/13);  total (11/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGAT  >  USA300TCH1516_ALE/1416333‑1416583
                                                                                                                              |                                                                                                                              
cATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                 <  2:234446/141‑1 (MQ=255)
cATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                 <  2:148642/141‑1 (MQ=255)
  tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                               <  1:29028/141‑1 (MQ=255)
      aaTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATa                                                                                                           >  2:155972/1‑141 (MQ=255)
        tCCCTCTTTAATTGTGTTCTATATTTAACCCAACGGTCAGTGTCTGGTGCATGTGGCATATACTTGAAATGCTTAATCTTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACa                                                                                                         <  1:291096/141‑1 (MQ=255)
             ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACt                                                                                                    >  2:113364/1‑141 (MQ=255)
                            atatTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATCTGTCat                                                                                     >  2:52647/1‑141 (MQ=255)
                                   aTTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaatacat                                                                             >  1:275254/1‑141 (MQ=255)
                                        aCGTTCAGTATACGGATGCAAATGCTCATACTTAAAATGATTAATATTACCTCGTTCAATGATTAGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTACGTTATGTATAAAAATAAATAATGt                                                                        >  2:97744/1‑141 (MQ=255)
                                                tataTGGTTGTAAATGTTGATACTTTAAATGATTAATATTAGCTCGTTCAATGATTTGACGTTCTTGTAGAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                <  2:223034/141‑1 (MQ=255)
                                                  tgcGGCTGCAAATGGTTATACGTGAAATTATTAATATTACCTCGTTCAATGATTTGACCTTGTTTGAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGt                                                              <  2:104601/139‑1 (MQ=255)
                                                         gCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATa                                                       <  1:113364/141‑1 (MQ=255)
                                                            aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATACATCAAACTTCACCATGTTCTAATACaa                                                    >  2:253884/1‑141 (MQ=255)
                                                                  tCAGACTTGAAATGATTAATATTACCTCGTTCAATTATTTGACCTTCTCTTAAAAGATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGc                                               <  2:172364/141‑1 (MQ=255)
                                                                     tACTTTAAATGATGAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGt                                            <  1:124034/141‑1 (MQ=255)
                                                                            aaTGATTAAGAGTACCTCGGTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTACGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAt                                     <  1:225903/141‑1 (MQ=255)
                                                                                          ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATAAATTGAtaa                       >  2:251140/1‑139 (MQ=255)
                                                                                                 cAATGATTTGACCGTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCa                <  1:18748/141‑1 (MQ=255)
                                                                                                  aaTGATTTGACCTTCTTTTAAAACATAATTGTCCTGCCAATATTTCAATACATGACTTAAGTTATGTTTGATAATAGATAATGTTTGACCATGTTGTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaa               >  2:13746/1‑141 (MQ=255)
                                                                                                   aTGATTTGACCTTCTTTTACAACCTAATTGTACTGACAATATTTCAATACAGAGCTTAAGTTATGTGTGAGAATAAATAATATTAGACAATCTTCTAATACAATAATCTTGAAATAATCAATAATTTAATTACCGtctaca              >  2:84427/1‑136 (MQ=255)
                                                                                                     gATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTACTAGATCCAGCACTTGATTACCGTTCAAAGc            >  2:47017/1‑141 (MQ=255)
                                                                                                       tttGACCTTCTTTTAAAACATAATTGTACTGACCATATTTCAAGAGATGAGTGAAATAATAATGTTGGTGATGTAATGTTTGGCCGTGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCAt          <  1:292316/141‑1 (MQ=255)
                                                                                                           aCCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCaa      >  1:260661/1‑141 (MQ=255)
                                                                                                               tcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatgat  <  1:155972/141‑1 (MQ=255)
                                                                                                                              |                                                                                                                              
CATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGAT  >  USA300TCH1516_ALE/1416333‑1416583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: