Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE77,3560AT22.2% 47.2 / 15.5 27N94I (AAC→ATC) USA300HOU_RS00350Monoacylglycerol lipase
Reads supporting (aligned to +/- strand):  ref base A (7/14);  new base T (3/3);  total (10/17)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATATTTAAATGAAGCGGGTTTTAGTGTTATTAGATATGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCAAATACAGTGAATGGTATTATAACTTCTGGAGCATTAACACGTTATAATAATAA  >  USA300TCH1516_ALE/77216‑77485
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atatTTAAATGAAGCGNGTTTTAGTGTTANTAGATATGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCaaa                                                                                                                                   >  1:286416/1‑141 (MQ=255)
atatTTAAATGAAGCGGGTTTTAGTGTTATTAGATATGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCaaa                                                                                                                                   >  2:333060/1‑141 (MQ=255)
    ttAAATGAAGCGGGTTTTAGTGTTATTAGATATGATCAAAGAGGGCATTGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACttt                                                                                                                               <  1:360728/141‑1 (MQ=255)
                       gtgtTATTAGATATGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTaa                                                                                                            <  2:257291/141‑1 (MQ=255)
                              taGATATGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATACTAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCa                                                                                                     <  1:61700/141‑1 (MQ=255)
                              taGATATGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCa                                                                                                     <  1:143478/141‑1 (MQ=255)
                                 atatGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAg                                                                                                  <  2:74217/141‑1 (MQ=255)
                                 atatGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAg                                                                                                  <  2:60958/141‑1 (MQ=255)
                                   atGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTa                                                                                                <  2:8874/141‑1 (MQ=255)
                                   atGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTa                                                                                                <  2:8871/141‑1 (MQ=255)
                                    tGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTAt                                                                                               <  1:365551/141‑1 (MQ=255)
                                     gATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAATCTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATg                                                                                              >  2:89172/1‑141 (MQ=255)
                                     gATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAATCTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATg                                                                                              >  2:72430/1‑141 (MQ=255)
                                     gATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAATCTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATg                                                                                              >  2:126974/1‑141 (MQ=255)
                                     gATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATg                                                                                              <  1:234126/141‑1 (MQ=255)
                                        cAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGggt                                                                                           <  1:233910/141‑1 (MQ=255)
                                          aaGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGggtgg                                                                                         >  1:84203/1‑141 (MQ=255)
                                          aaGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGggtgg                                                                                         >  1:411352/1‑141 (MQ=255)
                                                                ggCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGa                                                                   >  1:916/1‑141 (MQ=255)
                                                                ggCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGa                                                                   >  1:290307/1‑141 (MQ=255)
                                                                      cGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGaaa                                                             >  2:106189/1‑141 (MQ=255)
                                                                               tataGCAGTAGTAATGAAATTGTCTAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAATATCTAAAt                                                    <  2:250845/141‑1 (MQ=255)
                                                                                   gCAATAGTAATGAAATTGTCGAAGATCTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCAAATACAg                                                >  2:295500/1‑141 (MQ=255)
                                                                                           aaTGAAATTGTCGAAGATTTCGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCACATACAGTGAATGGt                                        >  1:344780/1‑141 (MQ=255)
                                                                                                 aTTGTCGAAGATTTAGGTGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCAAATACAGTGAATGGTATtata                                  <  1:219288/141‑1 (MQ=255)
                                                                                                                              ttATGTGAAGTCAATCTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCAAATACAGTGAATGGTATTATAACTTCTGGAGCATTAACACGTTataataa     <  1:89172/141‑1 (MQ=255)
                                                                                                                              ttATGTGAAGTCAATCTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCAAATACAGTGAATGGTATTATAACTTCTGGAGCATTAACACGTTataataa     <  1:72430/141‑1 (MQ=255)
                                                                                                                              ttATGTGAAGTCAATCTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCAAATACAGTGAATGGTATTATAACTTCTGGAGCATTAACACGTTataataa     <  1:126974/141‑1 (MQ=255)
                                                                                                                                 tgtgAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCAAATACAGTGAATGGTATTATAACTTCTGGAGCATTAACACGTTAtaataataa  <  1:88834/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                 
ATATTTAAATGAAGCGGGTTTTAGTGTTATTAGATATGATCAAAGAGGGCATGGTCGTTCTGAAGGCAAGCGTGCCTTTTATAGCAATAGTAATGAAATTGTCGAAGATTTAGATGCGATAATAAATTATGTGAAGTCAAACTTTGAAGGTAAAGTTTACTTAATCGGTCATAGTATGGGTGGTTATACAGTCACTTTATATGGAACGAAACATCCAAATACAGTGAATGGTATTATAACTTCTGGAGCATTAACACGTTATAATAATAA  >  USA300TCH1516_ALE/77216‑77485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑