Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 632,919 A→G G348G (GGA→GGG proP → Proline/betaine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE632,9190AG100.0% 155.2 / NA 41G348G (GGA→GGGproPProline/betaine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/22);  total (19/22)

AGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCATT  >  USA300TCH1516_ALE/632787‑633050
                                                                                                                                    |                                                                                                                                   
aGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTCGTGGGCTAACATTACTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCtt                                                                                                                             <  1:342255/141‑1 (MQ=255)
 gCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTa                                                                                                                            >  2:270534/1‑141 (MQ=255)
             ggTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGaa                                                                                                                >  2:46157/1‑141 (MQ=255)
                   aagaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACa                                                                                                          >  1:394261/1‑141 (MQ=255)
                     gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  1:231451/1‑141 (MQ=255)
                     gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  2:56614/1‑141 (MQ=255)
                     gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTAGATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  1:420229/1‑141 (MQ=255)
                           aTTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAgg                                                                                                  <  1:152472/141‑1 (MQ=255)
                               cTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAGTCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCg                                                                                              <  2:346647/141‑1 (MQ=255)
                               cTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCg                                                                                              >  2:11039/1‑141 (MQ=255)
                                 aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  1:282700/141‑1 (MQ=255)
                                 aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:318139/141‑1 (MQ=255)
                                 aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:387876/141‑1 (MQ=255)
                                 aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:387844/141‑1 (MQ=255)
                                         cTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGa                                                                                    >  2:404865/1‑141 (MQ=255)
                                            gtggGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGt                                                                                 <  1:382614/141‑1 (MQ=255)
                                            gtggGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGt                                                                                 <  1:51423/141‑1 (MQ=255)
                                              gggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGttt                                                                               <  2:278991/141‑1 (MQ=255)
                                              gggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGttt                                                                               >  1:171617/1‑141 (MQ=255)
                                               ggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGtttt                                                                              <  2:392715/141‑1 (MQ=255)
                                                gCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTa                                                                             <  1:164511/141‑1 (MQ=255)
                                                    aCATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGt                                                                         <  1:365793/141‑1 (MQ=255)
                                                    aCATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGt                                                                         <  2:383922/141‑1 (MQ=255)
                                                           tCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATaa                                                                  >  2:215743/1‑141 (MQ=255)
                                                           tCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATaa                                                                  >  2:266273/1‑141 (MQ=255)
                                                             aGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGa                                                                <  1:235696/141‑1 (MQ=255)
                                                             aGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGa                                                                <  1:165810/141‑1 (MQ=255)
                                                                 tcatcGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATc                                                            <  1:227405/141‑1 (MQ=255)
                                                                              gTTATTACATTCACAATCGTGGTGTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  1:3852/141‑1 (MQ=255)
                                                                              gTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  2:151026/141‑1 (MQ=255)
                                                                              gTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  2:151702/141‑1 (MQ=255)
                                                                                          acaATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACCTTTAatga                                   >  2:121816/1‑139 (MQ=255)
                                                                                           caATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATAtc                                  >  1:271020/1‑141 (MQ=255)
                                                                                           caATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATAtc                                  >  2:97291/1‑141 (MQ=255)
                                                                                                         aaTAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATAtt                    >  2:110913/1‑141 (MQ=255)
                                                                                                             gTAATCGGTATATTTATATTAGGGTTTTTCTTTTTTTCTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATTTTtggt                >  2:351948/1‑141 (MQ=255)
                                                                                                             gTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTtggt                >  1:353413/1‑141 (MQ=255)
                                                                                                             gTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATAtggt                >  2:396968/1‑141 (MQ=255)
                                                                                                              tAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTATGAAGCGACAATGCCAGGGGCGTTACCTACGATGTTTTACAGTCATATAAGGGATCGAACTTTATAAGTAACATTTAATATCTTTGTTGAGATATTtggtg               >  2:367447/1‑141 (MQ=255)
                                                                                                                        atttatattAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGcc     <  2:171617/141‑1 (MQ=255)
                                                                                                                           tatattAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCAtt  <  1:404865/141‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
AGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCATT  >  USA300TCH1516_ALE/632787‑633050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: