Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,018,630 Δ1 bp 7.7% coding (289/810 nt) mgt ← Monofunctional glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,018,6300T. 7.7% 221.6 / 13.9 78coding (289/810 nt)mgtMonofunctional glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (38/33);  new base . (2/4);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

AAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAG  >  USA300TCH1516_ALE/2018491‑2018768
                                                                                                                                           |                                                                                                                                          
aaaTAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATa                                                                                                                                           <  1:777063/141‑1 (MQ=255)
aaaTAATTTTTGACAACTTGTTGTGTAATGGTACTACCAACTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCCATCGGTGACGCGTGCAAATCGAATCCTTGATGATTGTAGAATCGTACAACTTCCATTGAAATa                                                                                                                                           >  2:717754/1‑141 (MQ=255)
   taatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAg                                                                                                                                        >  1:430710/1‑141 (MQ=255)
      tttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCa                                                                                                                                     >  1:280717/1‑141 (MQ=255)
         ttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCACGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCt                                                                                                                                  >  1:380991/1‑141 (MQ=255)
           gACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAAGCACCttt                                                                                                                                <  2:520493/141‑1 (MQ=255)
            aCAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTa                                                                                                                               >  1:715695/1‑141 (MQ=255)
            aCAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTa                                                                                                                               <  2:596868/141‑1 (MQ=255)
               aCTTGGTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACa                                                                                                                            <  1:601670/141‑1 (MQ=255)
                cTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTTAAATAAAGACACCTTTAACAt                                                                                                                           >  2:12704/1‑141 (MQ=255)
                   gttgtGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGTTTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTACCATAct                                                                                                                        >  2:433381/1‑141 (MQ=255)
                    ttgtGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTTTAGAATCGTTCATCTTCCATTGACATCAAGACACCTTTAACATActt                                                                                                                       >  2:219407/1‑140 (MQ=255)
                          aaTGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAGATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAACTAAAGGCACCTTTAACATCCTCTGGcct                                                                                                                 >  1:762521/1‑139 (MQ=255)
                          aaTGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTACCATACTCTGGCAt                                                                                                                 >  1:246956/1‑141 (MQ=255)
                            tGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGt                                                                                                               >  1:746770/1‑141 (MQ=255)
                             ggTACTACCACTTTGCACATCTCTGTCGTGAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGtt                                                                                                              <  2:175250/141‑1 (MQ=255)
                             ggTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAACCGAAACCAAAATAATGGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAAAATACTCTCGCACGtt                                                                                                              >  2:91329/1‑141 (MQ=255)
                                actacCACCTTGCACATCTCTGTCGCTGACCGCCGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCTTTCATCTCCCATGTAAATAAAGGCATCTTTAACATACTCTGGCATGTTATc                                                                                                           >  2:681840/1‑141 (MQ=255)
                                actacCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATATTATc                                                                                                           >  2:26559/1‑141 (MQ=255)
                                   accaccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGc                                                                                                        >  2:548090/1‑141 (MQ=255)
                                     caccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGAACCTTTAACATACTCTGGCATGTTATCAGCta                                                                                                      >  1:204705/1‑140 (MQ=255)
                                      accTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAAAAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGac                                                                                                    >  1:687751/1‑141 (MQ=255)
                                       ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGac                                                                                                    <  2:703343/141‑1 (MQ=255)
                                         ttGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGacac                                                                                                  <  1:620335/141‑1 (MQ=255)
                                             acaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGCATCGTTCATCTTCCATTACCATAAAGGCACCTTTACCATACTCTGGCTTGTTATCAGCTGACACacaa                                                                                              >  2:427140/1‑141 (MQ=255)
                                             acaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaaaa                                                                                              >  2:40720/1‑141 (MQ=255)
                                             acaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaaaa                                                                                              >  2:65271/1‑141 (MQ=255)
                                              caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAac                                                                                             <  1:408101/141‑1 (MQ=255)
                                                  tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTATACCTTTCAAATCGAATCCATTATGATTGTAGAATCGTTCATCTTCCATTCAAATAAAGGCACCTTTAACATACTCTCTCATCTTATCAGCGCACACAAAactact                                                                                         >  2:123951/1‑141 (MQ=255)
                                                       cGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACACACTCTGGCATGTTATCACCTGACACAAAACTACttttct                                                                                    >  2:538186/1‑139 (MQ=255)
                                                       cGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAAAAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtt                                                                                   <  1:457804/141‑1 (MQ=255)
                                                         ttAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                                  <  2:561001/140‑1 (MQ=255)
                                                         cTAATCGTTGAAAATAGAGCTCTAGTTGTACCTTTCAAATCGAATCCCTGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                                  <  1:147579/141‑1 (MQ=255)
                                                         cTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                                  <  1:686015/141‑1 (MQ=255)
                                                         cTAATCGTGGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                                  <  1:118882/141‑1 (MQ=255)
                                                          ttaTCGTTGATAATACAGCTCTAGTTGTTCCTTTTAAATCGAATTCATGATGATTGTAGTATCGTTCATCTTCTATTGAAATAAATGCACTTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtttt                                                                                 <  2:75576/139‑1 (MQ=255)
                                                          gagtCGTTGAAAATAATGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATATTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtttt                                                                                 <  2:208646/138‑1 (MQ=255)
                                                            aTCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCa                                                                               >  2:456506/1‑141 (MQ=255)
                                                            aTCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTCACACAAAACTACTtttcatttca                                                                               >  2:547771/1‑141 (MQ=255)
                                                             tCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCaa                                                                              <  1:478543/141‑1 (MQ=255)
                                                                 tGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTGTTGTCAAtttt                                                                          >  1:291392/1‑141 (MQ=255)
                                                                   aaaaGAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATTCTCTTGTATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTc                                                                        <  2:708935/141‑1 (MQ=255)
                                                                     aaTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCtt                                                                      >  1:95217/1‑141 (MQ=255)
                                                                      aTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCttg                                                                     >  1:456199/1‑140 (MQ=255)
                                                                        aaaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGt                                                                   >  1:377733/1‑141 (MQ=255)
                                                                        aaaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGt                                                                   <  2:280717/141‑1 (MQ=255)
                                                                               tAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCca                                                            <  2:293845/141‑1 (MQ=255)
                                                                                aGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTATTATTTTCAATTTTTCTTAGTTCCTCcac                                                           >  2:653904/1‑141 (MQ=255)
                                                                                 gTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCcaca                                                          <  1:40720/141‑1 (MQ=255)
                                                                                 gTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTGATCAGCTGACACAAAACTACTTTTATTTTCAATTGTTCTTAGTTCATCcaca                                                          <  1:427140/141‑1 (MQ=255)
                                                                                 gTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAAAAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACAt                                                         <  2:278749/141‑1 (MQ=255)
                                                                                       ccTTTCTAATCGAATACATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGTCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                                    <  2:676370/141‑1 (MQ=255)
                                                                                       ccTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                                    <  1:215481/141‑1 (MQ=255)
                                                                                         ttttAAATCAAATACATGATGATTGTAGAATCGTTCAGCCTCCATTGAAATAAAGGCACTTGTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcgcg                                                  <  2:239076/141‑1 (MQ=255)
                                                                                          ttCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCgt                                                 >  2:21595/1‑141 (MQ=255)
                                                                                              aaTCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTACTTCATCCACATTATCGCGTGTAg                                             >  2:136260/1‑141 (MQ=255)
                                                                                              aaTCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATAATCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTTTTAGTTTATCCACATTATTGCGTGTAg                                             >  2:664460/1‑141 (MQ=255)
                                                                                               aTCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAAAAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAt                                           <  1:489680/141‑1 (MQ=255)
                                                                                                ccGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAt                                           <  1:249770/140‑1 (MQ=255)
                                                                                                      ccATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAAATACTTTTATTTTCAATTTTTATTAGTTCATCGACATTACCGTCTGTAGATaaaaaa                                     >  2:394839/1‑141 (MQ=255)
                                                                                                        atgatgATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                                   <  1:490951/141‑1 (MQ=255)
                                                                                                        atgatgATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                                   <  1:552062/141‑1 (MQ=255)
                                                                                                        atgatgATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                                   <  1:408229/141‑1 (MQ=255)
                                                                                                        atgatgATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                                   <  1:382939/141‑1 (MQ=255)
                                                                                                            tgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCCCATTATCGCGTGTAGATAAAAAATACATg                               >  1:734337/1‑141 (MQ=255)
                                                                                                            tgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATg                               >  1:452814/1‑141 (MQ=255)
                                                                                                            tgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATg                               <  1:47962/141‑1 (MQ=255)
                                                                                                              atTGTAGAATCGTTCATCTTCCATTGAAAAAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTACCGCGTGTAGATAAAAAATACATGATa                            >  2:773798/1‑141 (MQ=255)
                                                                                                               ttGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATa                            <  1:548090/141‑1 (MQ=255)
                                                                                                                   aGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTAATTTTATTTTCACTGTTTCTTAGTTCATCCACATTATCGCGTCTAGATAAAACAAACATGCGACCaa                        >  2:408317/1‑141 (MQ=255)
                                                                                                                          ttCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTCCACTCGCGGGCGGATTTGCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACaa                 >  1:775599/1‑141 (MQ=255)
                                                                                                                           tCATCTTCCATTGAAAAAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATg               <  2:5056/141‑1 (MQ=255)
                                                                                                                               cTTCCTATGAAATAACGGCACCTTTAATAGATTCTTGCATGTTATCAGCTGACACAAAACTACTTTTATTTTTAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGa            <  2:268478/141‑1 (MQ=255)
                                                                                                                               cTTCCATTGAAATAAAGGCATCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGa            <  1:681840/141‑1 (MQ=255)
                                                                                                                                ttCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGAt           <  1:297708/141‑1 (MQ=255)
                                                                                                                                ggccATTGAAACAAAGGCACCTTTAACATACTCTGGCATGTTATTAGCTGACACAAAATTACTTATCCATTATATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGAt           <  2:7766/139‑1 (MQ=255)
                                                                                                                                        aaaTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATTATATGACTAAACAAAGCTCTAATGCTTa   >  1:253975/1‑141 (MQ=255)
                                                                                                                                         aaTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGCTAATGAttcg  >  1:682401/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAG  >  USA300TCH1516_ALE/2018491‑2018768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: