| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,018,634 | G→A | 8.9% | A95A (GCC→GCT) | mgt ← | Monofunctional glycosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,018,634 | 0 | G | A | 8.9% | 187.5 / 6.0 | 75 | A95A (GCC→GCT) | mgt | Monofunctional glycosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (36/31); new base A (2/5); total (38/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAG > USA300TCH1516_ALE/2018494‑2018768 | taatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAg > 1:430710/1‑141 (MQ=255) tttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCa > 1:280717/1‑141 (MQ=255) ttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCACGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCt > 1:380991/1‑141 (MQ=255) gACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAAGCACCttt < 2:520493/141‑1 (MQ=255) aCAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTa > 1:715695/1‑141 (MQ=255) aCAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTa < 2:596868/141‑1 (MQ=255) aCTTGGTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACa < 1:601670/141‑1 (MQ=255) cTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTTAAATAAAGACACCTTTAACAt > 2:12704/1‑141 (MQ=255) gttgtGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGTTTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTACCATAct > 2:433381/1‑141 (MQ=255) ttgtGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTTTAGAATCGTTCATCTTCCATTGACATCAAGACACCTTTAACATActt > 2:219407/1‑140 (MQ=255) aaTGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAGATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAACTAAAGGCACCTTTAACATCCTCTGGcct > 1:762521/1‑139 (MQ=255) aaTGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTACCATACTCTGGCAt > 1:246956/1‑141 (MQ=255) tGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGt > 1:746770/1‑141 (MQ=255) ggTACTACCACTTTGCACATCTCTGTCGTGAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGtt < 2:175250/141‑1 (MQ=255) ggTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAACCGAAACCAAAATAATGGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAAAATACTCTCGCACGtt > 2:91329/1‑141 (MQ=255) actacCACCTTGCACATCTCTGTCGCTGACCGCCGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCTTTCATCTCCCATGTAAATAAAGGCATCTTTAACATACTCTGGCATGTTATc > 2:681840/1‑141 (MQ=255) actacCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATATTATc > 2:26559/1‑141 (MQ=255) accaccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGc > 2:548090/1‑141 (MQ=255) caccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGAACCTTTAACATACTCTGGCATGTTATCAGCta > 1:204705/1‑140 (MQ=255) accTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAA‑AAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGac > 1:687751/1‑141 (MQ=255) ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGac < 2:703343/141‑1 (MQ=255) ttGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGacac < 1:620335/141‑1 (MQ=255) acaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGCATCGTTCATCTTCCATTACCATAAAGGCACCTTTACCATACTCTGGCTTGTTATCAGCTGACACacaa > 2:427140/1‑141 (MQ=255) acaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaaaa > 2:40720/1‑141 (MQ=255) acaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaaaa > 2:65271/1‑141 (MQ=255) caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAac < 1:408101/141‑1 (MQ=255) tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTATACCTTTCAAATCGAATCCATTATGATTGTAGAATCGTTCATCTTCCATTCAAATAAAGGCACCTTTAACATACTCTCTCATCTTATCAGCGCACACAAAactact > 2:123951/1‑141 (MQ=255) cGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACACACTCTGGCATGTTATCACCTGACACAAAACTACttttct > 2:538186/1‑139 (MQ=255) cGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAA‑AAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtt < 1:457804/141‑1 (MQ=255) ttAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt < 2:561001/140‑1 (MQ=255) cTAATCGTTGAAAATAGAGCTCTAGTTGTACCTTTCAAATCGAATCCCTGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt < 1:147579/141‑1 (MQ=255) cTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt < 1:686015/141‑1 (MQ=255) cTAATCGTGGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt < 1:118882/141‑1 (MQ=255) ttaTCGTTGATAATACAGCTCTAGTTGTTCCTTTTAAATCGAATTCATGATGATTGTAGTATCGTTCATCTTCTATTGAAATAAATGCACTTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtttt < 2:75576/139‑1 (MQ=255) gagtCGTTGAAAATAATGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATATTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtttt < 2:208646/138‑1 (MQ=255) aTCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCa > 2:456506/1‑141 (MQ=255) aTCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTCACACAAAACTACTtttcatttca > 2:547771/1‑141 (MQ=255) tCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCaa < 1:478543/141‑1 (MQ=255) tGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTGTTGTCAAtttt > 1:291392/1‑141 (MQ=255) aaaaGAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATTCTCTTGTATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTc < 2:708935/141‑1 (MQ=255) aaTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCtt > 1:95217/1‑141 (MQ=255) aTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCttg > 1:456199/1‑140 (MQ=255) aaaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGt > 1:377733/1‑141 (MQ=255) aaaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGt < 2:280717/141‑1 (MQ=255) tAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCca < 2:293845/141‑1 (MQ=255) aGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTATTATTTTCAATTTTTCTTAGTTCCTCcac > 2:653904/1‑141 (MQ=255) gTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCcaca < 1:40720/141‑1 (MQ=255) gTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTGATCAGCTGACACAAAACTACTTTTATTTTCAATTGTTCTTAGTTCATCcaca < 1:427140/141‑1 (MQ=255) gTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAA‑AAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACAt < 2:278749/141‑1 (MQ=255) ccTTTCTAATCGAATACATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGTCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg < 2:676370/141‑1 (MQ=255) ccTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg < 1:215481/141‑1 (MQ=255) ttttAAATCAAATACATGATGATTGTAGAATCGTTCAGCCTCCATTGAAATAAAGGCACTTGTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcgcg < 2:239076/141‑1 (MQ=255) ttCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCgt > 2:21595/1‑141 (MQ=255) aaTCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTACTTCATCCACATTATCGCGTGTAg > 2:136260/1‑141 (MQ=255) aaTCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATAATCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTTTTAGTTTATCCACATTATTGCGTGTAg > 2:664460/1‑141 (MQ=255) aTCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAA‑AAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAt < 1:489680/141‑1 (MQ=255) ccGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAt < 1:249770/140‑1 (MQ=255) ccATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAAATACTTTTATTTTCAATTTTTATTAGTTCATCGACATTACCGTCTGTAGATaaaaaa > 2:394839/1‑141 (MQ=255) atgatgATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa < 1:408229/141‑1 (MQ=255) atgatgATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa < 1:552062/141‑1 (MQ=255) atgatgATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa < 1:382939/141‑1 (MQ=255) atgatgATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa < 1:490951/141‑1 (MQ=255) tgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCCCATTATCGCGTGTAGATAAAAAATACATg > 1:734337/1‑141 (MQ=255) tgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATg > 1:452814/1‑141 (MQ=255) tgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATg < 1:47962/141‑1 (MQ=255) atTGTAGAATCGTTCATCTTCCATTGAAA‑AAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTACCGCGTGTAGATAAAAAATACATGATa > 2:773798/1‑141 (MQ=255) ttGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATa < 1:548090/141‑1 (MQ=255) aGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTAATTTTATTTTCACTGTTTCTTAGTTCATCCACATTATCGCGTCTAGATAAAACAAACATGCGACCaa > 2:408317/1‑141 (MQ=255) ttCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTCCACTCGCGGGCGGATTTGCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACaa > 1:775599/1‑141 (MQ=255) tCATCTTCCATTGAAA‑AAAAGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATg < 2:5056/141‑1 (MQ=255) cTTCCTATGAAATAACGGCACCTTTAATAGATTCTTGCATGTTATCAGCTGACACAAAACTACTTTTATTTTTAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGa < 2:268478/141‑1 (MQ=255) cTTCCATTGAAATAAAGGCATCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGa < 1:681840/141‑1 (MQ=255) ttCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGAt < 1:297708/141‑1 (MQ=255) ggccATTGAAACAAAGGCACCTTTAACATACTCTGGCATGTTATTAGCTGACACAAAATTACTTATCCATTATATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGAt < 2:7766/139‑1 (MQ=255) aaaTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATTATATGACTAAACAAAGCTCTAATGCTTa > 1:253975/1‑141 (MQ=255) aaTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGCTAATGAttcg > 1:682401/1‑139 (MQ=255) | TAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAG > USA300TCH1516_ALE/2018494‑2018768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |