Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_012417 40 G→T 20.6% intergenic (–/‑240) – / → USA300HOU_RS14890 –/replication protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_012417400GT20.6% 2090.3 / inf 1088intergenic (–/‑240)–/USA300HOU_RS14890–/replication protein
Reads supporting (aligned to +/- strand):  ref base G (442/401);  new base T (125/103);  total (568/520)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 1100 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAATGTGGTAAT  >  NC_012417/1‑180
                                                                                                                                         |                                                                                                                                            
aaacgtgtttttgtgcgtgaaaggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAtata                                                                                                                                           <  1:764632‑M1/43‑1 (MQ=255)
 aacgtgtttttgtgcgtgaaaggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATAAagatga                                                                                                                                          <  1:430937‑M1/44‑3 (MQ=255)
       tttttgtgcgtgaaaggaaaatttgacaataaaaaaccccagttatattagtaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAAc                                                                                                                                    >  1:193209‑M1/92‑141 (MQ=255)
        ttttgtgcgtgaaaggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGCAAATCAATATAATAAAaca                                                                                                                                   <  2:741219‑M1/51‑2 (MQ=255)
        ttttgtgcgggaaaggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACg                                                                                                                                   >  1:474352‑M1/91‑141 (MQ=255)
        ttttgggcgtgaaaggaaaaattgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAATATAATAAAACg                                                                                                                                   >  2:754872‑M1/91‑141 (MQ=255)
         tttgtgcgtgaaaggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAATATAATAAAACGt                                                                                                                                  <  2:660415‑M1/52‑1 (MQ=255)
          ttgttcgtgaaagcaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGtt                                                                                                                                 >  1:509070‑M1/89‑141 (MQ=255)
           tgtgcgtgaaaggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTa                                                                                                                                <  2:516753‑M1/54‑1 (MQ=255)
            gtgcgtgaaaggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTaa                                                                                                                               >  1:342812‑M1/87‑141 (MQ=255)
             tgcgtgaaaggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTaaa                                                                                                                              >  1:211859‑M1/86‑141 (MQ=255)
                      ggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATgaa                                                                                                                     <  2:591021‑M1/65‑2 (MQ=255)
                      ggaaaatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACAGAAAATCAAGATAATAAAACGTTAAAAGATATGAc                                                                                                                     <  1:144574‑M1/65‑1 (MQ=255)
                       gaaaagttgataataaaaaatcccagttatattattaaggtgtcgaatattaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACg                                                                                                                    >  2:283786‑M1/76‑141 (MQ=255)
                          aatttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGaaa                                                                                                                 >  2:220649‑M1/73‑141 (MQ=255)
                           atttgacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGaaaa                                                                                                                <  2:34082‑M1/70‑1 (MQ=255)
                           atttgacaataaaaaaccccagttatattattaaggtgtagaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAAATAATAAAACGTTAAAAGATATGACAaaaa                                                                                                                <  2:578963‑M1/70‑1 (MQ=255)
                               gacaataaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTgg                                                                                                            <  2:228648‑M1/74‑1 (MQ=255)
                                  aataaaaaacctaagttatattattgaggtgtcgaatcttaaataatagtgggagtctttatatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGaa                                                                                                         >  2:766122‑M1/65‑141 (MQ=255)
                                    taaaaatccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAGCGTTAAAAGATATGACGACAAGTGGGAaac                                                                                                       >  2:323464‑M1/63‑141 (MQ=255)
                                    aaaaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAaac                                                                                                       >  1:525390‑M1/63‑141 (MQ=255)
                                      aaaaaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTCCGACAACCATCACCGATACCCAAGAGAATAAAACGTTAAAAGAGATGACGAAAAGTGGGAaacaa                                                                                                     >  2:557878‑M1/61‑141 (MQ=255)
                                       aaaaccccaattatattattaaggtttcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAATATAATAAAACGTTAAACGATATGACGAAAAGTGGGAaacaac                                                                                                    >  1:424728‑M1/60‑141 (MQ=255)
                                         aaccccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGc                                                                                                  >  1:303431‑M1/58‑141 (MQ=255)
                                             ccagttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCAt                                                                                              >  2:713069‑M1/54‑141 (MQ=255)
                                               agttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATTACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCAtga                                                                                            <  2:473545‑M1/90‑2 (MQ=255)
                                                gttatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGga                                                                                           <  1:414855‑M1/91‑1 (MQ=255)
                                                  tatattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGgaga                                                                                         <  1:705870‑M1/93‑1 (MQ=255)
                                                   atattattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATACAGCTCCCCCTAAGGTAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGgagag                                                                                        >  1:369425‑M1/48‑141 (MQ=255)
                                                     attattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaa                                                                                      <  2:193150‑M1/96‑1 (MQ=255)
                                                     attattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaa                                                                                      >  1:570527‑M1/46‑141 (MQ=255)
                                                     agtattaagttgtcgaattttaaataatactgggggtttttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGCCCCATCGCCCATGGAGagaa                                                                                      >  2:481600‑M1/46‑141 (MQ=255)
                                                      ttattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaa                                                                                     <  1:491980‑M1/97‑1 (MQ=255)
                                                       tattaatatgtcgaatcttaaataatactgcgtgtctttttatATGCAATATAATACTACTAGAAGTATAACCCAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaaa                                                                                    >  1:441475‑M1/44‑141 (MQ=255)
                                                       tattaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaaa                                                                                    >  1:517765‑M1/44‑141 (MQ=255)
                                                        attaaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaaag                                                                                   >  2:700026‑M1/43‑141 (MQ=255)
                                                        attaaggtgtagaatattaaataatactgggggtatttttatATGAAATATAATACTACTAGAAGTATAACAGAAAATAAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaaag                                                                                   >  2:111172‑M1/43‑141 (MQ=255)
                                                         ttaaagtgacaaatattaaataataatgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaaaga                                                                                  >  2:440157‑M1/42‑141 (MQ=255)
                                                          taaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaaagaa                                                                                 >  2:640919‑M1/41‑141 (MQ=255)
                                                           aaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaaagaaa                                                                                >  1:401138‑M1/40‑141 (MQ=255)
                                                           aaggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGagaaaagaaa                                                                                >  2:570997‑M1/40‑141 (MQ=255)
                                                            aggtgtcgaatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAgaaaagaaaa                                                                               <  1:554037‑M1/103‑1 (MQ=255)
                                                                ggcgaatctgaaataacactaggggtattgttatATGCAAGATAATACTACGAGAAGTATAACCGAAAATCAAGATATAAAAACGTTAAAAGATAGGACGAAAAGGGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGa                                                                           >  1:462019‑M1/35‑141 (MQ=255)
                                                                    aatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAAGCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGAGGGAAACAACGCACATCGAAAGAAAAGAAAatagagaaa                                                                       <  2:123871‑M1/111‑5 (MQ=255)
                                                                    aatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATGGATAAt                                                                       <  1:730382‑M1/111‑1 (MQ=255)
                                                                    aatcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAAt                                                                       <  1:227227‑M1/111‑1 (MQ=255)
                                                                     atcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACAAAAAGTGGGAAACAACGCACATGGAGAGAAAAGAAAATAGATAATg                                                                      <  2:457523‑M1/112‑1 (MQ=255)
                                                                      tcttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGt                                                                     >  1:613928‑M1/29‑141 (MQ=255)
                                                                        ttaaataatactggtggtctttttatATGCAATATAATACGACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACTAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTaa                                                                   >  2:354028‑M1/27‑141 (MQ=255)
                                                                        ttaaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAAAGTaa                                                                   <  1:754267‑M1/115‑1 (MQ=255)
                                                                          aaatcatcatgggggtctttttatATGCCATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGc                                                                 >  1:175426‑M1/25‑141 (MQ=255)
                                                                          aaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAACTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGc                                                                 <  2:501670‑M1/117‑1 (MQ=255)
                                                                          aaataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGc                                                                 <  1:245581‑M1/117‑1 (MQ=255)
                                                                          aaataatactgggggaatgttgttATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGc                                                                 <  2:684712‑M1/117‑1 (MQ=255)
                                                                            ataatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTa                                                               <  2:723899‑M1/119‑1 (MQ=255)
                                                                             taatactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTAt                                                              >  1:134867‑M1/22‑141 (MQ=255)
                                                                                tactgggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCa                                                           >  1:94138‑M1/19‑141 (MQ=255)
                                                                                    gggggtctttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGata                                                       <  1:35677‑M1/127‑1 (MQ=255)
                                                                                            ttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATGTACTAGaa                                               <  1:627656‑M1/135‑1 (MQ=255)
                                                                                            ttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGaa                                               <  1:718153‑M1/135‑1 (MQ=255)
                                                                                            ttttatATGCAATATAATACTACTAGAAGTATAACCGAAAATAAAGATAATAAAACGATAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGAAGAGAAAAGAAAATAGAAAATGTAAACTAtacagatataatcgaa                                               <  2:375034‑M1/135‑7 (MQ=255)
                                                                                                  aTGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCAAGGAGAGAAAAGAAAAAAGATAATGTATGGTATGCAGATATAATAGAAATTTTa                                         >  2:630777/1‑141 (MQ=255)
                                                                                                    gCAATATAATACTACTAGAAGTATAACCGAAAATCAAGAAAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTaaa                                       >  2:267265/1‑141 (MQ=255)
                                                                                                      aataaaataaTACAAGAAGTATAACCGAAAATCAAGAAAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCACATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTaaaaa                                     <  2:414855/141‑1 (MQ=255)
                                                                                                       atatAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAAt                                    >  2:648221/1‑141 (MQ=255)
                                                                                                          taataCTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCaa                                 <  2:177629/141‑1 (MQ=255)
                                                                                                           aataatACGAGAAGTATAACCGAAAATCAAGATAATAAAAGGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCaaa                                <  2:306281/141‑1 (MQ=255)
                                                                                                            ataCTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCaaaa                               >  1:273809/1‑141 (MQ=255)
                                                                                                            ataCTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTATAAAAATCaaaa                               >  2:548727/1‑141 (MQ=255)
                                                                                                            ataCTACTAGAAGTATAACCGAAAAGCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCaaaa                               >  1:779192/1‑141 (MQ=255)
                                                                                                             tactacTAGAAGTATAACCGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCaaaaa                              >  1:262063/1‑141 (MQ=255)
                                                                                                              actactAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAg                             <  2:61167/141‑1 (MQ=255)
                                                                                                               ctactaGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAgg                            >  1:53910/1‑141 (MQ=255)
                                                                                                                  ctaGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCtt                         >  1:125501/1‑141 (MQ=255)
                                                                                                                     gAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTa                      >  2:253150/1‑141 (MQ=255)
                                                                                                                      aaGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAGTAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAACGGCTCTTaa                     >  1:289341/1‑141 (MQ=255)
                                                                                                                       aGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAAt                    >  2:8841/1‑141 (MQ=255)
                                                                                                                        gTATAACCGAAAATAAAGATAATAAAACGTTAAAAGATATGAAGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATg                   <  2:293941/141‑1 (MQ=255)
                                                                                                                          ataACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAACAACCTTTCAATGTa                 >  2:669378/1‑141 (MQ=255)
                                                                                                                           taACCGAAACTCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTaa                <  1:694465/141‑1 (MQ=255)
                                                                                                                           taACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTaa                >  1:107711/1‑141 (MQ=255)
                                                                                                                           taAACGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTCTGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTaa                <  2:401347/141‑1 (MQ=255)
                                                                                                                           aaaCCGAAAATCAAGATAAAAAGACGTTAAAAGATAGGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAAGCAAAAAGGCTTTTAATGTaa                <  2:532710/140‑1 (MQ=255)
                                                                                                                            aaCCGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTaaa               >  2:776338/1‑141 (MQ=255)
                                                                                                                            aaCCGAAAAGCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGAGAAACAACGCACATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGtcac               <  2:138708/141‑4 (MQ=255)
                                                                                                                             aCCGAAAATCAACATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTaaaa              <  1:157926/141‑1 (MQ=255)
                                                                                                                              ccGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTaaaaa             >  1:592076/1‑140 (MQ=255)
                                                                                                                              ccGAAAATCAAGATAATAAAACGTTAAAAGATATGACGGAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAAc             >  1:653552/1‑141 (MQ=255)
                                                                                                                              ccGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAAc             >  1:453003/1‑141 (MQ=255)
                                                                                                                               cGAAAATCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACa            >  2:206171/1‑141 (MQ=255)
                                                                                                                               cGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACa            >  1:677581/1‑141 (MQ=255)
                                                                                                                                 aaaaTCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAAt          <  1:325672/141‑1 (MQ=255)
                                                                                                                                  aaaTCAATATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACaagg         >  1:602559/1‑139 (MQ=255)
                                                                                                                                   aaggaaGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAAtgt        <  2:159231/137‑1 (MQ=255)
                                                                                                                                   aaTCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGATATGCAGATATACTAGAAATTATAAAAAAAACATAACCTTTTAATGTAAAAAAAtgt        >  2:312068/1‑141 (MQ=255)
                                                                                                                                    aTCAAGATAAGAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAAtgtg       <  1:100937/141‑1 (MQ=255)
                                                                                                                                     tCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAATGCgg      >  1:315875/1‑141 (MQ=255)
                                                                                                                                      cAAGATAATAAAAATATAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAATGTGGt     <  1:668357/141‑1 (MQ=255)
                                                                                                                                       aaGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAATGTGGTa    >  1:386141/1‑141 (MQ=255)
                                                                                                                                        aGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAATGTGGTaa   >  1:637195/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATGCAATATAATACTACTAGAAGTATAACCGAAAATCAAGATAATAAAACGTTAAAAGATATGACGAAAAGTGGGAAACAACGCCCATGGAGAGAAAAGAAAATAGATAATGTAAGCTATGCAGATATACTAGAAATTTTAAAAATCAAAAAGGCTTTTAATGTAAAACAATGTGGTAAT  >  NC_012417/1‑180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑