Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,430,685 Δ1 bp 5.8% intergenic (‑109/‑276) suhB ← / → USA300HOU_2294 inositol‑phosphate phosphatase/transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,430,6850T. 5.8% 442.7 / 21.5 138intergenic (‑109/‑276)suhB/USA300HOU_2294inositol‑phosphate phosphatase/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (67/61);  new base . (3/5);  total (71/67)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01

Only 100 of 138 total aligned reads displayed.

TCTATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAATAT  >  CP000730/2430548‑2430822
                                                                                                                                         |                                                                                                                                          
tCTATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTACAAGAATAAACACTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCAc                                                                                                                                         <  2:1402587/141‑1 (MQ=255)
tCTATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCAc                                                                                                                                         <  1:1586486/141‑1 (MQ=255)
  tATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGt                                                                                                                                       <  1:967613/141‑1 (MQ=255)
  tATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGCACGTg                                                                                                                                      <  2:1235696/141‑1 (MQ=255)
  tATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGCACGTg                                                                                                                                      <  1:1244857/141‑1 (MQ=255)
      tGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACTGGACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTa                                                                                                                                  >  1:104584/1‑141 (MQ=255)
      tGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTa                                                                                                                                  >  1:1364585/1‑141 (MQ=255)
       tcTGTACTTTTTTACCTGTCATTTCATTCACCACTTCTCATATCATTATCCTTTTATTATTTCCATATATTAAAAGAATAATCAATACAACTGAAGACTTCTTCATTTTATGCATAAAAATATCGGCCAGTCACGTGCCAg                                                                                                                                 <  2:491616/140‑1 (MQ=255)
       gCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAg                                                                                                                                 <  2:330327/141‑1 (MQ=255)
        cTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGc                                                                                                                                <  1:939789/141‑1 (MQ=255)
         tGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGcc                                                                                                                               <  2:1195385/141‑1 (MQ=255)
          gTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCg                                                                                                                              >  2:359041/1‑141 (MQ=255)
          gTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCg                                                                                                                              <  1:973052/141‑1 (MQ=255)
          gTAATGTTTTATCTGTCATTTAGTCGAATACTTTGCGAATTATTATTTTGGTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGCACGTGACTAGCCg                                                                                                                              >  2:494030/1‑141 (MQ=255)
           tAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGCACGTGACTAGCCGa                                                                                                                             <  1:1586165/141‑1 (MQ=255)
             aTGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACa                                                                                                                           <  1:1040195/141‑1 (MQ=255)
             aTGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACa                                                                                                                           >  1:288363/1‑141 (MQ=255)
             aTGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATATAAAAAAAAAAAAAATCGGCTAGTCACGTGCTAGCCGACa                                                                                                                           <  2:178276/141‑1 (MQ=255)
                ttttATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAAt                                                                                                                        <  1:654959/141‑1 (MQ=255)
                ttttATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAAt                                                                                                                        >  2:927178/1‑141 (MQ=255)
                ttttATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAAt                                                                                                                        >  2:2316/1‑141 (MQ=255)
                    aTCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACGCGATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACTTTCTAGCCGACTAATAgaa                                                                                                                    >  2:576512/1‑141 (MQ=255)
                    aTCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaa                                                                                                                    >  1:430302/1‑141 (MQ=255)
                    aTCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaa                                                                                                                    >  1:18359/1‑141 (MQ=255)
                    aTCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTCCATTTTATACATAAAAAAATCGGCTAATTAAGTGCTAGTCGAAAAATaaaa                                                                                                                    >  2:744545/1‑141 (MQ=255)
                     tCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaaa                                                                                                                   >  2:215235/1‑141 (MQ=255)
                     tCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaaa                                                                                                                   <  1:361355/141‑1 (MQ=255)
                      cTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaaag                                                                                                                  >  1:690859/1‑141 (MQ=255)
                      cTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaaag                                                                                                                  <  2:27803/141‑1 (MQ=255)
                       tGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGCACGTGACTAGCCGatttttttatgca                                                                                                                 <  1:1474789/141‑13 (MQ=38)
                         tCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaaaggaa                                                                                                               >  2:741852/1‑141 (MQ=255)
                           aTTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaaaggaaag                                                                                                             <  2:1014163/141‑1 (MQ=255)
                              tCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAaagtaa                                                                                                          <  1:1018426/141‑1 (MQ=255)
                               cGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGNGCTAGCCGACAANTAGAAAGGAaagtaag                                                                                                         <  1:966855/141‑1 (MQ=255)
                                  ccaccaCTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAgaaactattgctcatca                                                                                                      >  1:983313/1‑128 (MQ=255)
                                   caccacTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGtaat                                                                                                     <  1:1295322/141‑1 (MQ=255)
                                    accacTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGtaata                                                                                                    >  2:409238/1‑141 (MQ=255)
                                        cTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTaataaata                                                                                                <  1:1366431/141‑1 (MQ=255)
                                        cTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAAGCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTaataaata                                                                                                <  1:16088/141‑1 (MQ=255)
                                          tctcATATCATTATCATTTTATTATTATCATTTAGTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATAtt                                                                                              >  2:495046/1‑141 (MQ=255)
                                          tctcATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATAtt                                                                                              <  1:899439/141‑1 (MQ=255)
                                           ctcATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTg                                                                                             >  1:1391977/1‑141 (MQ=255)
                                            tcATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGa                                                                                            >  1:397439/1‑141 (MQ=255)
                                             cATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAaagtaagt                                                                                                        >  2:1234347/1‑128 (MQ=255)
                                             cATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAaagtaagt                                                                                                        <  1:1234347/128‑1 (MQ=255)
                                             cATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGaa                                                                                           >  1:525599/1‑141 (MQ=255)
                                              atatCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAg                                                                                          >  2:544793/1‑141 (MQ=255)
                                                   attatcattTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGAtgtt                                                                                     <  1:507623/141‑1 (MQ=255)
                                                     tatcattTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGAtgttgt                                                                                   <  1:205895/141‑1 (MQ=255)
                                                          ttttATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGt                                                                              <  1:86953/141‑1 (MQ=255)
                                                          ttttATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGt                                                                              <  1:1296612/141‑1 (MQ=255)
                                                          ttttATTATTACCCTATATTAAAAGAATCAACAATACAACTGAACACTTCTTCATTTTATTCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGt                                                                              <  2:720321/141‑1 (MQ=255)
                                                            ttattattaCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTaa                                                                            >  2:1342140/1‑141 (MQ=255)
                                                             tattattaCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAAc                                                                           <  2:591475/141‑1 (MQ=255)
                                                                 attaCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGa                                                                       >  1:72705/1‑141 (MQ=255)
                                                                  ttaCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGaa                                                                      <  2:1124035/141‑1 (MQ=255)
                                                                   taCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTACGTAATAAATATTGAAGATGTTGTGATGTAACATGAAc                                                                     >  2:1334895/1‑141 (MQ=255)
                                                                   taCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAAc                                                                     >  2:148251/1‑141 (MQ=255)
                                                                   taCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAAc                                                                     >  2:892764/1‑141 (MQ=255)
                                                                   taCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAAc                                                                     <  2:1603301/141‑1 (MQ=255)
                                                                      ccTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGAt                                                                  <  1:687041/141‑1 (MQ=255)
                                                                         atatTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTaa                                                               <  2:1164372/141‑1 (MQ=255)
                                                                         atatTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTaa                                                               >  1:393653/1‑141 (MQ=255)
                                                                         atatTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTaa                                                               >  1:363875/1‑141 (MQ=255)
                                                                            ttAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAg                                                            >  1:1593897/1‑141 (MQ=255)
                                                                             tAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGc                                                           <  2:929509/141‑1 (MQ=255)
                                                                               aaaGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTa                                                         >  1:1654171/1‑141 (MQ=255)
                                                                                    aTCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCAAGTCACGTGCTAGTCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGt                                                    >  2:1654192/1‑141 (MQ=255)
                                                                                      caacaaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTta                                                  <  2:1358958/141‑1 (MQ=255)
                                                                                      caacaaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTta                                                  >  1:110807/1‑141 (MQ=255)
                                                                                      caacaaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTta                                                  >  1:1404863/1‑141 (MQ=255)
                                                                                        acaaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTtata                                                >  1:891684/1‑141 (MQ=255)
                                                                                        acaaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTtata                                                >  2:143765/1‑141 (MQ=255)
                                                                                              cAACTGAAGACTTCTTCATTTTATGCCTAAAAAAATCGGCTAGACCCGTGAAAGACGCCAACTAAATAGTATACATAGTAATAAATCTTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGctc                                          <  1:576512/141‑1 (MQ=255)
                                                                                                aCTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTa                                        <  2:1349599/141‑1 (MQ=255)
                                                                                                    aaGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTAcccc                                    <  2:575057/141‑1 (MQ=255)
                                                                                                        cttcttcttTTTATGCATAAAACATTCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGTAAAGTATGTAATAAATATTGAAGATGTTGTGCTGTAACTTTAACGATTAAAAGCTATATGTTATATAGCTCTACCGCTTTg                                >  2:835104/1‑141 (MQ=255)
                                                                                                        cttcttCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTg                                >  1:1548878/1‑141 (MQ=255)
                                                                                                        cttcttCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTg                                <  2:470018/141‑1 (MQ=255)
                                                                                                          tcttcATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGtt                              <  1:1642454/141‑1 (MQ=255)
                                                                                                                ttttATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCg                        <  1:187249/141‑1 (MQ=255)
                                                                                                                 tttATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGc                       <  2:525599/141‑1 (MQ=255)
                                                                                                                    aTGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTcc                    >  2:432658/1‑141 (MQ=255)
                                                                                                                     tGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTccc                   >  1:579481/1‑141 (MQ=255)
                                                                                                                       cATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTccccc                 >  2:501851/1‑141 (MQ=255)
                                                                                                                         tAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTg               >  2:174092/1‑141 (MQ=255)
                                                                                                                         tAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTg               >  2:1277911/1‑141 (MQ=255)
                                                                                                                          aaaaaaaTCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGt              <  1:1266677/141‑1 (MQ=255)
                                                                                                                           tatttgtCGGCTAGCACGTGACTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCCGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGtt             >  2:1272693/7‑141 (MQ=255)
                                                                                                                           aaaaaaTCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGtt             >  1:1453523/1‑141 (MQ=255)
                                                                                                                           aaaaaaTCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGtt             >  1:368215/1‑141 (MQ=255)
                                                                                                                             aaaaTCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTAc           <  2:306290/141‑1 (MQ=255)
                                                                                                                              aaaTCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGACAAATCCAAAGATATCTGTTATATTGATCTACCACTTTGTTCAATCGCTCCCACTGTTACa          >  1:150943/1‑141 (MQ=255)
                                                                                                                                aTCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAg        >  2:1357906/1‑141 (MQ=255)
                                                                                                                                aTCGGCTAGTCACGTGCTAGCCGACAAACAGAAAGAAAAGTAAGTAATAAATATTTAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAg        <  1:1306828/141‑1 (MQ=255)
                                                                                                                                 tCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGt       <  1:892764/141‑1 (MQ=255)
                                                                                                                                 tCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGt       >  2:1611456/1‑141 (MQ=255)
                                                                                                                                 tCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGt       <  1:1187689/141‑1 (MQ=255)
                                                                                                                                  cGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTa      <  1:820745/141‑1 (MQ=255)
                                                                                                                                    gCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAat    >  2:791975/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TCTATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTGCTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAATAT  >  CP000730/2430548‑2430822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: