Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 96,901:1 +G 8.0% intergenic (+1417/+62) gloB → / ← USA300HOU_0095 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073096,9011.G 8.0% 700.0 / 55.7 162intergenic (+1417/+62)gloB/USA300HOU_0095hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (83/66);  new base G (5/8);  total (88/74)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 171 total aligned reads displayed.

ATGACGTGTCAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAG  >  CP000730/96761‑97041
                                                                                                                                             |                                                                                                                                             
aTGACGTGTCAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGat                                                                                                                                                <  1:546701/141‑1 (MQ=255)
aTGACGTGTCAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGat                                                                                                                                                <  2:836458/141‑1 (MQ=255)
 tGACGTGTCAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGata                                                                                                                                              >  1:136802/1‑141 (MQ=255)
  gACGTGTCAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGatat                                                                                                                                             >  1:300933/1‑141 (MQ=255)
  gACGTGTCAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGatat                                                                                                                                             <  1:153581/141‑1 (MQ=255)
         cAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTaa                                                                                                                                      >  1:1161405/1‑141 (MQ=255)
           gtgttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAAtt                                                                                                                                    <  1:1438088/141‑1 (MQ=255)
             gttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGa                                                                                                                                 <  2:1260009/141‑1 (MQ=255)
             gttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGa                                                                                                                                 >  1:1110555/1‑141 (MQ=255)
               tgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGaaa                                                                                                                               <  1:1304539/141‑1 (MQ=255)
                gtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGaaaa                                                                                                                              >  2:1028329/1‑141 (MQ=255)
                    ttATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAatcat                                                                                                                          <  2:131656/141‑1 (MQ=255)
                       tatGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCAtctc                                                                                                                       >  1:1600177/1‑141 (MQ=255)
                         tGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAatatcttac                                                                                                                      <  1:372560/141‑8 (MQ=37)
                            cGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAATATCTTActgc                                                                                                                  <  1:366329/141‑1 (MQ=37)
                            cGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCActgc                                                                                                                  <  1:138462/141‑1 (MQ=255)
                            cGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATAATCTCActgc                                                                                                                  >  1:484471/1‑141 (MQ=255)
                             gTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCActgct                                                                                                                 >  1:1066982/1‑141 (MQ=255)
                              tGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCActgctg                                                                                                                <  2:798319/141‑1 (MQ=255)
                               gAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGAGTGAGTCGCTCAAGAAGGATGAAGATGAAAATAGAAACAGCATTAAGATATCTTCTAATTGAAAATCATCTCACTGCGGt                                                                                                               >  1:1653465/1‑141 (MQ=255)
                                 aTTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGttt                                                                                                             >  1:894247/1‑141 (MQ=255)
                                 aTTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGttt                                                                                                             <  2:1520604/141‑1 (MQ=255)
                                   ttttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGttttt                                                                                                           <  1:1072193/141‑1 (MQ=255)
                                    tttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGtttttt                                                                                                          >  1:518240/1‑141 (MQ=255)
                                    tttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGtttttt                                                                                                          >  2:142891/1‑141 (MQ=255)
                                      tAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAatatcttact                                                                                                                     >  1:783126/1‑121 (MQ=37)
                                      tAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAatatcttact                                                                                                                     <  2:783126/129‑9 (MQ=37)
                                       aGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                       >  1:875277/1‑141 (MQ=255)
                                        gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                      <  2:922645/141‑1 (MQ=255)
                                        gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                      <  2:1613878/141‑1 (MQ=255)
                                          tctAAATAGTAAAAGATAGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAATATCTTACTGCTGtttctattttca                                                                                                    <  2:332234/141‑10 (MQ=18)
                                           ctAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGtt                                                                                                   >  2:1498653/1‑141 (MQ=255)
                                            tAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGttt                                                                                                  >  1:1320919/1‑141 (MQ=255)
                                             aaaTAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                 <  1:119738/141‑1 (MQ=255)
                                             aaaTAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                 <  2:152227/141‑1 (MQ=255)
                                             aaaTAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                 <  1:1328246/141‑1 (MQ=255)
                                                   tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCTTACAATTTGGATGATTATTAATATAGAAACTTCAGTAATATTTTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                           >  1:328073/1‑141 (MQ=37)
                                                   tataAGATTGAAAAAGTTGTTACTGCTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTCACTGCCGTTTTTTAAAGGTTTATACATc                                                                                           >  1:1148286/1‑141 (MQ=255)
                                                     taAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAATCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCAt                                                                                         <  2:803487/141‑1 (MQ=255)
                                                     taAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCAt                                                                                         >  2:1351985/1‑141 (MQ=255)
                                                       aGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATcc                                                                                       >  1:1468566/1‑141 (MQ=255)
                                                          ttGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCctct                                                                                    >  1:269324/1‑141 (MQ=255)
                                                             aaaaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTaaa                                                                                 >  2:229054/1‑141 (MQ=255)
                                                              aaaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAat                                                                                >  1:1009765/1‑141 (MQ=255)
                                                               aaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAatt                                                                               >  2:1375284/1‑141 (MQ=255)
                                                                  gttgttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAattatt                                                                            >  2:414878/1‑141 (MQ=255)
                                                                    tgttACTGTTTTAAATNATCACGAAGAAGNCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGCTTTCTAAAGGTTTATACCTCATCCTCTAAATTATTTa                                                                          >  1:1216855/1‑141 (MQ=255)
                                                                    tgttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTa                                                                          <  1:74073/141‑1 (MQ=255)
                                                                     gttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaa                                                                         >  1:476679/1‑141 (MQ=255)
                                                                      ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                        <  1:1138351/141‑1 (MQ=255)
                                                                      ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                        >  2:904215/1‑141 (MQ=255)
                                                                      ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                        >  2:330675/1‑141 (MQ=255)
                                                                        aCTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTtaaaa                                                                      >  1:557850/1‑141 (MQ=255)
                                                                          tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                    >  2:1576231/1‑141 (MQ=255)
                                                                          tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                    <  1:420656/141‑1 (MQ=255)
                                                                          tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                    >  2:1169780/1‑141 (MQ=255)
                                                                                 aaTGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATg                                                             >  1:1130384/1‑141 (MQ=255)
                                                                                   tGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGt                                                           >  2:966962/1‑141 (MQ=255)
                                                                                     aTCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAACAATAATTAATGGTAt                                                         >  2:1422887/1‑141 (MQ=255)
                                                                                         cGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGa                                                     >  1:468674/1‑141 (MQ=255)
                                                                                         cGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGa                                                     <  1:1521319/141‑1 (MQ=255)
                                                                                          gATGAAGTCATTCAAGAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAg                                                    <  1:1373962/141‑1 (MQ=255)
                                                                                           aTGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGc                                                   >  1:1338643/1‑141 (MQ=255)
                                                                                             gAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCAc                                                 >  2:912739/1‑141 (MQ=255)
                                                                                              aaGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCActg                                                                                                                   <  1:292040/74‑1 (MQ=255)
                                                                                               aGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTATTTTTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGt                                               >  2:1085231/1‑141 (MQ=255)
                                                                                                     tCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCg                                         <  2:512155/141‑1 (MQ=255)
                                                                                                     tCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCg                                         <  2:746332/141‑1 (MQ=255)
                                                                                                       aaTAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGAc                                       <  1:1351985/141‑1 (MQ=255)
                                                                                                       aaTAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGAc                                       <  1:614278/141‑1 (MQ=255)
                                                                                                       aaTAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGAc                                       <  1:401715/141‑1 (MQ=255)
                                                                                                        aTAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACt                                      >  2:680293/1‑141 (MQ=255)
                                                                                                        aTAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACt                                      >  2:1479556/1‑141 (MQ=255)
                                                                                                         tAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACtt                                     <  2:610298/141‑1 (MQ=255)
                                                                                                          aaGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTTTTTTTTTTTACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACttt                                    >  2:569091/1‑141 (MQ=38)
                                                                                                            gAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAt                                  >  1:121811/1‑141 (MQ=255)
                                                                                                             aaTGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATAGTATTTGAGCACGTTTATCTACTTTagt                                 >  2:1403040/1‑139 (MQ=255)
                                                                                                                gATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgact                              >  2:148096/1‑141 (MQ=255)
                                                                                                                 aTTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAACATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgactg                             >  2:1583433/1‑141 (MQ=255)
                                                                                                                  ttATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgactga                            >  1:1428907/1‑141 (MQ=255)
                                                                                                                     tGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                         >  1:1631611/1‑141 (MQ=255)
                                                                                                                     tGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                         >  2:272498/1‑141 (MQ=255)
                                                                                                                     tGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                         >  2:1297080/1‑141 (MQ=255)
                                                                                                                     tGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                         <  2:684140/141‑1 (MQ=255)
                                                                                                                      gAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAtt                        <  1:1631022/141‑1 (MQ=255)
                                                                                                                           tAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCaa                   <  1:387838/141‑1 (MQ=255)
                                                                                                                           tAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCaa                   >  2:375424/1‑141 (MQ=255)
                                                                                                                             gAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAtt                 <  1:744318/141‑1 (MQ=255)
                                                                                                                              aaaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                <  2:235583/141‑1 (MQ=255)
                                                                                                                              aaaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                >  1:1174946/1‑141 (MQ=255)
                                                                                                                               aaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc               >  2:595716/1‑141 (MQ=255)
                                                                                                                               aaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc               >  1:694785/1‑141 (MQ=255)
                                                                                                                                aCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc              <  2:104274/141‑1 (MQ=255)
                                                                                                                                aCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTAGTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc              <  2:428889/141‑1 (MQ=255)
                                                                                                                                  agtagTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt            <  2:1392613/138‑1 (MQ=255)
                                                                                                                                  agcagTGAGATGATTTTCAATTAGAAAATATCTTACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt            >  1:58610/1‑141 (MQ=37)
                                                                                                                                  agcagTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt            <  2:638498/141‑1 (MQ=255)
                                                                                                                                     agTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTCCCAATTTCCATTTc         >  2:18170/1‑141 (MQ=255)
                                                                                                                                         aGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAATGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGc     >  2:1113602/1‑141 (MQ=255)
                                                                                                                                            tatTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAg  <  1:1482579/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
ATGACGTGTCAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAG  >  CP000730/96761‑97041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: