Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 96,912:1 +A 6.4% intergenic (+1428/+51) gloB → / ← USA300HOU_0095 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073096,9121.A 6.4% 733.6 / 37.3 172intergenic (+1428/+51)gloB/USA300HOU_0095hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (87/74);  new base A (5/6);  total (92/80)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

Only 100 of 173 total aligned reads displayed.

GTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCAA  >  CP000730/96772‑97049
                                                                                                                                              |                                                                                                                                         
  gttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGa                                                                                                                                         <  2:1260009/141‑1 (MQ=255)
  gttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGa                                                                                                                                         >  1:1110555/1‑141 (MQ=255)
   ttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGaa                                                                                                                                        >  2:914316/1‑141 (MQ=255)
    tgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGaaa                                                                                                                                       <  1:1304539/141‑1 (MQ=255)
     gtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGaaaa                                                                                                                                      >  2:1028329/1‑141 (MQ=255)
           atatGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAatatct                                                                                                                                 >  2:366329/1‑137 (MQ=38)
              tGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAatatcttac                                                                                                                              <  2:1398389/141‑8 (MQ=37)
              tGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAatatcttac                                                                                                                              <  1:372560/141‑8 (MQ=37)
                  gTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCActgct                                                                                                                         >  1:1066982/1‑141 (MQ=255)
                   tGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCActgctg                                                                                                                        <  2:798319/141‑1 (MQ=255)
                      aTTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAATATCTTACTGCTGttt                                                                                                                     <  1:590330/141‑1 (MQ=25)
                        ttttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGttttt                                                                                                                   <  1:1072193/141‑1 (MQ=255)
                         tttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGtttttt                                                                                                                  >  1:518240/1‑141 (MQ=255)
                           tAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAatatcttact                                                                                                                             >  1:783126/1‑121 (MQ=37)
                           tAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTCAATTAGAAAatatcttact                                                                                                                             <  2:783126/129‑9 (MQ=37)
                            aGCTCTAAATAGTATTAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                               <  1:1378564/141‑1 (MQ=255)
                            aGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                               <  1:272788/141‑1 (MQ=255)
                            aGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                               <  1:1182415/141‑1 (MQ=255)
                            aGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                               >  1:875277/1‑141 (MQ=255)
                             gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                              <  2:1613878/141‑1 (MQ=255)
                             gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                              >  1:1613878/1‑141 (MQ=255)
                             gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                              <  2:922645/141‑1 (MQ=255)
                              ctctAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAgg                                                                                                             <  2:648623/141‑1 (MQ=255)
                                 tAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGttt                                                                                                          >  1:1320919/1‑141 (MQ=255)
                                  aaacaGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                         <  2:230375/137‑1 (MQ=255)
                                  aaaTAGTATAAGATTGAAAAAGGTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAAGTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                         <  1:1546516/141‑1 (MQ=255)
                                        tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCTTACAATTTGGATGATTATTAATATAGAAACTTCAGTAATATTTTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                   >  1:328073/1‑141 (MQ=37)
                                        tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                   <  1:48/141‑1 (MQ=255)
                                        tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                   <  1:234899/141‑1 (MQ=255)
                                        tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                   >  1:1335852/1‑141 (MQ=255)
                                        tataAGATTGAAAAAGTTGTTACTGCTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTCACTGCCGTTTTTTAAAGGTTTATACATc                                                                                                   >  1:1148286/1‑141 (MQ=255)
                                          taAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAATCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCAt                                                                                                 <  2:803487/141‑1 (MQ=255)
                                          taAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCAt                                                                                                 >  2:1351985/1‑141 (MQ=255)
                                            aGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATcc                                                                                               >  2:1534053/1‑141 (MQ=255)
                                            aGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATcc                                                                                               >  1:902411/1‑141 (MQ=255)
                                             gATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCct                                                                                              <  1:390993/141‑1 (MQ=255)
                                               ttGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCctct                                                                                            >  2:862269/1‑141 (MQ=255)
                                                tGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTa                                                                                           <  1:1646748/141‑1 (MQ=255)
                                                   aaaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAat                                                                                        >  1:47622/1‑141 (MQ=255)
                                                   aaaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAat                                                                                        >  1:1009765/1‑141 (MQ=255)
                                                    aaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAatt                                                                                       >  2:1375284/1‑141 (MQ=255)
                                                       gttgttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAattatt                                                                                    >  2:414878/1‑141 (MQ=255)
                                                           ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                                >  2:330675/1‑141 (MQ=255)
                                                           ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                                <  1:1138351/141‑1 (MQ=255)
                                                            tACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaaa                                                                               <  1:1422583/141‑1 (MQ=255)
                                                             aCTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTtaaaa                                                                              >  1:557850/1‑141 (MQ=255)
                                                              cTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaat                                                                             >  2:532895/1‑141 (MQ=255)
                                                               tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                            >  2:1169780/1‑141 (MQ=255)
                                                               tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                            >  2:1576231/1‑141 (MQ=255)
                                                               tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                            <  1:420656/141‑1 (MQ=255)
                                                                 ttttAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaataat                                                                          <  1:1463608/141‑1 (MQ=255)
                                                                  tttAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAAtaatt                                                                         <  2:1520023/141‑1 (MQ=255)
                                                                      aaTGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATg                                                                     >  1:1130384/1‑141 (MQ=255)
                                                                        tGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGt                                                                   >  2:966962/1‑141 (MQ=255)
                                                                          aTCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAACAATAATTAATGGTAt                                                                 >  2:1422887/1‑141 (MQ=255)
                                                                            cACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTAttt                                                               >  2:577693/1‑141 (MQ=255)
                                                                              cGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGa                                                             >  1:468674/1‑141 (MQ=255)
                                                                                aTGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGc                                                           >  1:1338643/1‑141 (MQ=255)
                                                                                  gAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCAc                                                         >  2:912739/1‑141 (MQ=255)
                                                                                   aaGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCActg                                                                                                                           <  1:292040/74‑1 (MQ=255)
                                                                                    aGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTATTTTTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGt                                                       >  2:1085231/1‑141 (MQ=255)
                                                                                       cATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTa                                                    <  2:1335852/141‑1 (MQ=255)
                                                                                          tCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCg                                                 <  2:746332/141‑1 (MQ=255)
                                                                                             aTAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACt                                              >  2:680293/1‑141 (MQ=255)
                                                                                                 gcaTGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAt                                          <  2:61155/139‑1 (MQ=255)
                                                                                                 gAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAt                                          >  1:121811/1‑141 (MQ=255)
                                                                                                  aaTGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATAGTATTTGAGCACGTTTATCTACTTTagt                                         >  2:1403040/1‑139 (MQ=255)
                                                                                                     gATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgact                                      >  2:148096/1‑141 (MQ=255)
                                                                                                      aTTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAACATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgactg                                     >  2:1583433/1‑141 (MQ=255)
                                                                                                       ttATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgactga                                    >  1:1428907/1‑141 (MQ=255)
                                                                                                          tGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                 >  2:272498/1‑141 (MQ=255)
                                                                                                          tGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                 >  2:147178/1‑141 (MQ=255)
                                                                                                           gAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAtt                                <  1:1631022/141‑1 (MQ=255)
                                                                                                            aaaaTAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTa                               <  1:150798/141‑1 (MQ=255)
                                                                                                            aaaaTAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTa                               <  1:541558/141‑1 (MQ=255)
                                                                                                             aaaTAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTAc                              <  2:252395/141‑1 (MQ=255)
                                                                                                                tAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTGCCTGCGGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCaa                           >  1:380362/1‑141 (MQ=255)
                                                                                                                  gAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAtt                         <  1:744318/141‑1 (MQ=255)
                                                                                                                   aaaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                        >  1:638498/1‑141 (MQ=255)
                                                                                                                   aaaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                        >  1:1174946/1‑141 (MQ=255)
                                                                                                                    aaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                       >  2:1480104/1‑141 (MQ=255)
                                                                                                                    aaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                       >  2:595716/1‑141 (MQ=255)
                                                                                                                    aaCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                       >  1:694785/1‑141 (MQ=255)
                                                                                                                     aCAGCAGTGAGATGATTTTCAATTAGAAAATATCTTACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc                      >  1:771958/1‑141 (MQ=37)
                                                                                                                     aCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc                      <  2:104274/141‑1 (MQ=255)
                                                                                                                     aCAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTAGTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc                      <  2:428889/141‑1 (MQ=255)
                                                                                                                      cagcagTGAGATGATTTTCAATTAGAAAATATCTTACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCa                     >  1:1204955/1‑141 (MQ=37)
                                                                                                                       agcagTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt                    <  2:1521136/141‑1 (MQ=255)
                                                                                                                          agTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTCCCAATTTCCATTTc                 >  2:18170/1‑141 (MQ=255)
                                                                                                                              aGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGc             >  2:1305621/1‑141 (MQ=255)
                                                                                                                               gATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGcc            <  2:1133017/141‑1 (MQ=255)
                                                                                                                                 tatTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAg          <  1:1482579/141‑1 (MQ=255)
                                                                                                                                      ttCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGatat      <  2:466898/141‑1 (MQ=255)
                                                                                                                                      ttCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGatat      <  1:1362581/141‑1 (MQ=255)
                                                                                                                                       tCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATAtt     >  1:731975/1‑141 (MQ=255)
                                                                                                                                         tAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCa   <  1:1297080/141‑1 (MQ=255)
                                                                                                                                         tAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCa   <  1:147178/141‑1 (MQ=255)
                                                                                                                                          aaTTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCaa  <  2:894237/141‑1 (MQ=255)
                                                                                                                                          aaTTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCaa  <  1:363594/141‑1 (MQ=255)
                                                                                                                                          aaTTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCaa  >  2:20616/1‑141 (MQ=255)
                                                                                                                                              |                                                                                                                                         
GTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGATATTTTCTAATTGAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCAA  >  CP000730/96772‑97049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: