Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,211,670 C→A 6.6% intergenic (‑396/+150) USA300HOU_2086 ← / ← yidC hypothetical protein/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,211,6700CA 6.6% 468.4 / 14.3 140intergenic (‑396/+150)USA300HOU_2086/yidChypothetical protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (79/51);  new base A (6/3);  total (85/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 146 total aligned reads displayed.

CATATACTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGAC  >  CP000730/2211531‑2211810
                                                                                                                                           |                                                                                                                                             
            cacTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAatcatc                                                                                                                                 >  1:368197/1‑141 (MQ=255)
                gatgaATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTAc                                                                                                                             <  1:798945/141‑1 (MQ=255)
                gatgaATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAAGATGATTTCAAATGTGAAAGTCATCCTAc                                                                                                                             <  1:196976/141‑1 (MQ=37)
                 atgaATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTAct                                                                                                                            <  2:1345105/141‑1 (MQ=255)
                     aTCATTGTGTCAAGATGACATTGCGATGAAGAATGACAGCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTActgctg                                                                                                                        >  1:876727/1‑141 (MQ=255)
                      tCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGt                                                                                                                       >  2:612057/1‑141 (MQ=255)
                      tCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGt                                                                                                                       >  2:612070/1‑141 (MQ=255)
                      tCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGt                                                                                                                       >  2:145763/1‑141 (MQ=255)
                      tCATTGTGTCAAGATGACAGTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGt                                                                                                                       <  2:1102968/141‑1 (MQ=255)
                        aTTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTTTTTTTTTTTCACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTCCTGCTGttt                                                                                                                     >  2:1590956/1‑141 (MQ=255)
                        aTTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGttt                                                                                                                     >  2:249582/1‑141 (MQ=255)
                           gtgtCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCATTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTCTCTa                                                                                                                  >  2:372018/1‑141 (MQ=255)
                            tgtCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTAt                                                                                                                 >  1:1577611/1‑141 (MQ=255)
                             gtCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTTTTTTTTTTAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTAtt                                                                                                                >  2:1061608/1‑141 (MQ=255)
                             gtCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTAtt                                                                                                                >  1:626224/1‑141 (MQ=255)
                              tCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTAc                                                                                                                             <  2:1246640/127‑1 (MQ=255)
                              tCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTAc                                                                                                                             >  1:1246640/1‑127 (MQ=255)
                               cAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTa                                                                                                              <  1:1451765/141‑1 (MQ=255)
                               aaaGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTa                                                                                                              <  2:4105/140‑1 (MQ=255)
                                 aGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATc                                                                                                            >  2:1297480/1‑141 (MQ=255)
                                  gATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATca                                                                                                           >  1:969413/1‑141 (MQ=255)
                                  gATGACATTGCGATGAAGAATGACAACTCTGTTATTAAACACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGTTGTTTATATTTTTca                                                                                                           >  2:151300/1‑141 (MQ=255)
                                      aCATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACata                                                                                                       <  2:1386327/141‑1 (MQ=255)
                                         ttGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTg                                                                                                    >  2:1005430/1‑141 (MQ=255)
                                          tGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGt                                                                                                   <  1:1321800/141‑1 (MQ=255)
                                              aTGAATAATGACAACTCTGTTATTAACCACGTTTTACATACTTAAAACCCCTTAATATTATTGCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATaa                                                                                               <  1:152223/141‑1 (MQ=255)
                                                gaagaaTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAAtg                                                                                             <  2:126756/141‑1 (MQ=255)
                                                     aTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGaca                                                                                        >  2:133035/1‑141 (MQ=255)
                                                     aTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGaca                                                                                        <  1:819811/141‑1 (MQ=255)
                                                      tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac                                                                                       >  2:1639426/1‑141 (MQ=255)
                                                      tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac                                                                                       >  1:398340/1‑141 (MQ=255)
                                                      tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac                                                                                       >  2:1655339/1‑141 (MQ=255)
                                                       gACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGGTGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATTGTGTGACACt                                                                                      >  1:193582/1‑141 (MQ=255)
                                                        aCAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTGTATTTATCACATATTGTATAATGTGACACTa                                                                                     >  1:780885/1‑141 (MQ=255)
                                                           aCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAAGATGATTTCAAATGTGAAAGtcatcc                                                                                                                                <  2:424109/95‑2 (MQ=21)
                                                            ctctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGtt                                                                                 >  1:393530/1‑141 (MQ=255)
                                                            ctctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGtt                                                                                 <  2:477645/141‑1 (MQ=255)
                                                            ctctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGtt                                                                                 >  1:976376/1‑141 (MQ=255)
                                                              ctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTc                                                                               <  2:1354718/141‑1 (MQ=255)
                                                               tGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCg                                                                              >  1:1048386/1‑141 (MQ=255)
                                                                gTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCCCATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGc                                                                             <  1:68963/141‑1 (MQ=255)
                                                                gTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGc                                                                             >  1:1068462/1‑141 (MQ=255)
                                                                 ttattaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCt                                                                            <  1:414893/141‑1 (MQ=255)
                                                                   attaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCGGGTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTAt                                                                          >  2:1423475/1‑141 (MQ=255)
                                                                      aaCCACTTTTTACATACTGAAGACTCGTTAATGTTCGTTCAAATAACAACAGCAGTAGGATGATTTTCACTTTTGAAATCATCTTACTGCTGGTTCTATATATCACATATTGGATAATATGACACTAAGTTTCGCTAttaa                                                                       >  2:1467505/1‑139 (MQ=255)
                                                                      aaCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGcaaataa                                                                       <  2:1141715/141‑7 (MQ=255)
                                                                      aaCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGa                                                                       >  1:444242/1‑141 (MQ=255)
                                                                      aaCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGa                                                                       >  1:177396/1‑141 (MQ=255)
                                                                      aaCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGa                                                                       >  2:119941/1‑141 (MQ=255)
                                                                       aCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGaa                                                                      >  1:1121442/1‑141 (MQ=255)
                                                                        ccACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCttcaatag                                                                     <  1:431886/141‑8 (MQ=255)
                                                                        ccACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAg                                                                     >  2:967671/1‑141 (MQ=255)
                                                                         cACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTACTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGc                                                                    <  1:1202658/141‑1 (MQ=255)
                                                                          aCTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCttcaatagcg                                                                   <  1:145763/141‑10 (MQ=255)
                                                                          aCTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCg                                                                   <  2:290240/141‑1 (MQ=255)
                                                                             ttttACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGATATTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATTTTTCACTAAGTTTCGCTATTGAAGCGtaa                                                                >  2:812199/1‑141 (MQ=255)
                                                                             ttttACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGaaa                                                                >  2:184841/1‑141 (MQ=255)
                                                                                 aCATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAaata                                                            <  2:150313/141‑1 (MQ=255)
                                                                                        gAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTgcg                                                     <  2:1568936/141‑1 (MQ=255)
                                                                                          aggatacggcctattatTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGcc                                                   <  2:476552/130‑1 (MQ=255)
                                                                                          aggatacggcctattatTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGcc                                                   <  2:258380/130‑1 (MQ=255)
                                                                                           aaCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATTATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGccc                                                  <  1:524780/141‑1 (MQ=255)
                                                                                            aCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCt                                                 >  1:479496/1‑141 (MQ=255)
                                                                                            aCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCt                                                 >  2:683966/1‑141 (MQ=255)
                                                                                              tCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTgcgcaaaaa                                               <  2:444242/141‑6 (MQ=255)
                                                                                                gTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATaa                                             >  1:412307/1‑141 (MQ=255)
                                                                                                gTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATaa                                             >  2:114410/1‑141 (MQ=255)
                                                                                                       ttattTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTaaa                                      <  2:876727/141‑1 (MQ=255)
                                                                                                       ttattTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTaaa                                      >  2:537372/1‑141 (MQ=255)
                                                                                                            tCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTAt                                 >  2:69334/1‑141 (MQ=255)
                                                                                                              aaaTAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATca                               >  2:792546/1‑140 (MQ=255)
                                                                                                              aaaTAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCt                               >  2:750106/1‑141 (MQ=255)
                                                                                                                 tAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTcc                            >  2:1184740/1‑140 (MQ=255)
                                                                                                                 tAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCa                            <  2:1458520/141‑1 (MQ=255)
                                                                                                                   gaaaCAGCAGTAAGATGATTTCAAATGTGAAAGTCATCCTACTGCTGTTTTTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTaaa                                      >  1:684356/2‑129 (MQ=21)
                                                                                                                   gaaaCAGCAGTAAGATGATTTCAAATGTGAAAGTCATCCTACTGCTGTTTTTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTaaa                                      <  2:684356/128‑1 (MQ=21)
                                                                                                                   aaaaCAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAAc                          <  2:222299/141‑1 (MQ=255)
                                                                                                                   aaaaCAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAAc                          >  1:239011/1‑141 (MQ=255)
                                                                                                                    aaaCAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACt                         >  1:43785/1‑141 (MQ=255)
                                                                                                                    aaaCAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACt                         >  1:912924/1‑141 (MQ=255)
                                                                                                                    aaaCAGCAGTAAGATGATTTCAAATGTGAAAGTCATCCTACTGCTGTTTTTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGAGCGCCCTATAAAGTTAAAATTATGTTCAACt                         >  1:1349887/1‑141 (MQ=21)
                                                                                                                     aaCAGCAGTAGGATGACTCTCACATTTGAAAACACCTTACTGCTGTTTCAATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATAGAAGCGAAAAAAAAAGTGCGCAATATAAAGTTAAAATTACCATCaaatc                        >  2:772595/1‑138 (MQ=255)
                                                                                                                       cagcagTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACtttt                      >  2:961296/1‑141 (MQ=255)
                                                                                                                       cagcagTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACtttt                      <  2:199220/141‑1 (MQ=255)
                                                                                                                         gcagTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAg                    >  2:105149/1‑141 (MQ=255)
                                                                                                                          cagTAGGATGACATTAACATTTGAAATCATCTAACAGCTGATTCTATTTATCACATATTGAATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAgg                   >  2:737394/1‑141 (MQ=255)
                                                                                                                             tAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTg                >  2:1443319/1‑141 (MQ=255)
                                                                                                                              taagatgatttCAAATGTGAAAGTCATCCTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAATTTATCTTCAACTTTTAGGGTg                >  1:1565532/9‑141 (MQ=37)
                                                                                                                              aGGATGACTTTCACATTTTAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGc               <  1:510551/141‑1 (MQ=255)
                                                                                                                              aGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGc               <  2:845606/141‑1 (MQ=255)
                                                                                                                               ggATGACTTTCACATATGAAATCCTCAAACTGCTATTGCAACCTAACACATATTGGATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGca              <  1:151300/141‑1 (MQ=255)
                                                                                                                                gATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATCTTTCGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGcac             <  1:29465/141‑1 (MQ=255)
                                                                                                                                gATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGcac             <  2:976376/141‑1 (MQ=255)
                                                                                                                                 gatgatttCAAATGTGAAAGTCATCCTACTGCTGTTTTTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATGATCTTCAACTTTTAGGGTGcac             >  1:340743/6‑141 (MQ=37)
                                                                                                                                 aTGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGcaca            <  1:656941/141‑1 (MQ=255)
                                                                                                                                   gACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACatt          >  1:343399/1‑141 (MQ=255)
                                                                                                                                    aCTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACatta         <  2:334591/141‑1 (MQ=255)
                                                                                                                                     cTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACattat        >  1:118585/1‑141 (MQ=255)
                                                                                                                                         cacaTTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTgg    <  1:10612/141‑1 (MQ=255)
                                                                                                                                          acaTTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGa   >  2:499533/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                             
CATATACTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGAC  >  CP000730/2211531‑2211810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: