Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,243,499 T→A 5.4% intergenic (+77/+32) USA300HOU_2114 → / ← pyrG hypothetical protein/CTP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,243,4990TA 5.4% 537.3 / 12.8 159intergenic (+77/+32)USA300HOU_2114/pyrGhypothetical protein/CTP synthase
Reads supporting (aligned to +/- strand):  ref base T (76/74);  new base A (4/5);  total (80/79)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

AATTATGTATACTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAATTGACAAGCAATAAAG  >  CP000730/2243360‑2243639
                                                                                                                                           |                                                                                                                                             
aaTTATGTATACTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTa                                                                                                                                              <  1:561703/141‑1 (MQ=255)
    aTGTATACTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTAATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCATTGTTAAACat                                                                                                                                          <  2:1304714/141‑2 (MQ=255)
     tGTATACTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCATTGTTAAACatt                                                                                                                                         <  2:919357/141‑3 (MQ=255)
     tGTATACTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAAt                                                                                                                                         >  1:1360425/1‑141 (MQ=255)
      gaataCTATAATATTTATACACAAATGATTGAAATGAAAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAATATAATATAAAAAAAAAAAAAAAAACCGGTACTTTCAATGTTTAACAATg                                                                                                                                        <  2:1549829/139‑1 (MQ=255)
      gTATACTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATg                                                                                                                                        >  1:659614/1‑141 (MQ=255)
      gTATACTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATg                                                                                                                                        >  2:435743/1‑141 (MQ=255)
         taCTATGACATTTCTACCCAAATGATTGAAATGACAAGAGCCCCAGACTTATAGACGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAACAACCGGTCCTTTACATGTTTACCAAtacaa                                                                                                                                     >  2:151140/1‑137 (MQ=255)
         taCTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGaaa                                                                                                                                     >  1:313767/1‑141 (MQ=255)
          aCTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAg                                                                                                                                    <  2:1365230/141‑1 (MQ=255)
             aTGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTAc                                                                                                                                 >  1:800588/1‑141 (MQ=255)
              tGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTAcc                                                                                                                                >  1:1214186/1‑141 (MQ=255)
                 cATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGt                                                                                                                             >  2:340210/1‑141 (MQ=255)
                     tataCACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTtta                                                                                                                         >  2:1524620/1‑141 (MQ=255)
                         aacaAATGATTGAAATGACAAGAGACTTATAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAg                                                                                                                    <  2:511596/140‑1 (MQ=255)
                           caAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCa                                                                                                                  <  1:220823/141‑1 (MQ=255)
                              aTGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAat                                                                                                               >  2:1530651/1‑141 (MQ=255)
                               tGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAatt                                                                                                              >  1:264733/1‑141 (MQ=255)
                                gATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAattt                                                                                                             <  2:391476/141‑1 (MQ=255)
                                gATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAattt                                                                                                             >  1:290722/1‑141 (MQ=255)
                                  ttGAAATGACAAGAGGCTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAatttat                                                                                                           >  2:9271/1‑141 (MQ=255)
                                   tGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAatttatt                                                                                                          >  2:1324856/1‑141 (MQ=255)
                                   tGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAatttatt                                                                                                          <  1:726065/141‑1 (MQ=255)
                                   tGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAatttatt                                                                                                          <  1:1607560/141‑1 (MQ=255)
                                    gAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAatttattt                                                                                                         >  2:1075810/1‑141 (MQ=255)
                                     aaaTGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAatttattta                                                                                                        >  2:1400763/1‑141 (MQ=255)
                                         gACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAATACCGGTACTTTCAATGTTTATTAATGAAAGTACCGGTTTTATTAGCAAATTTATTTAtttt                                                                                                    <  2:998416/141‑1 (MQ=255)
                                           cAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTttgt                                                                                                  >  2:561095/1‑141 (MQ=255)
                                           cAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTttgt                                                                                                  >  1:167128/1‑141 (MQ=255)
                                           cAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTttgt                                                                                                  >  2:365764/1‑141 (MQ=255)
                                             agagACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTttgttg                                                                                                <  1:733094/141‑1 (MQ=255)
                                             agagACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTttgttg                                                                                                >  1:276852/1‑141 (MQ=255)
                                              gagaCTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGa                                                                                               <  2:1360425/141‑1 (MQ=255)
                                                gaCTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGata                                                                                             >  2:1578977/1‑141 (MQ=255)
                                                     aGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTa                                                                                        >  2:589404/1‑141 (MQ=255)
                                                         ttATAAAAGTAACTATTGTTATTTCATACCTTGCCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAATTACCGGTTTTATTATCAAATTTATTTATTTTGTTGATATTTTAATGa                                                                                    <  2:710418/141‑1 (MQ=255)
                                                          tataAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGaa                                                                                   >  2:884286/1‑141 (MQ=255)
                                                           ataAAAGTCACTATTGTTATTTCATACCTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGc                                                                                 <  2:835960/141‑1 (MQ=255)
                                                            taAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGc                                                                                 <  1:1415105/141‑1 (MQ=255)
                                                            taAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGc                                                                                 >  2:243631/1‑141 (MQ=255)
                                                            taAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGc                                                                                 <  1:176133/141‑1 (MQ=255)
                                                            taAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGc                                                                                 >  1:1321457/1‑141 (MQ=255)
                                                               aaGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTc                                                                              <  1:1063160/141‑1 (MQ=255)
                                                                aGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCa                                                                             >  1:1299011/1‑141 (MQ=255)
                                                                 gTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCaa                                                                            <  2:439267/141‑1 (MQ=255)
                                                                    aCTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATaa                                                                         >  1:1642914/1‑141 (MQ=255)
                                                                           ttATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGAttt                                                                  <  2:1299011/141‑1 (MQ=255)
                                                                           ttATTTAATAACTTGTCAATAAAATAATATTAAAATTATACGAAAGACCGGTACTATCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATACATGAttt                                                                  <  2:363020/141‑1 (MQ=255)
                                                                             aTTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTaa                                                                >  2:1279989/1‑141 (MQ=255)
                                                                              tttCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTaaa                                                               <  1:644867/141‑1 (MQ=255)
                                                                               ttCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTaaaa                                                              <  2:1383697/141‑1 (MQ=255)
                                                                               ttCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTaaaa                                                              >  2:399170/1‑141 (MQ=255)
                                                                               ttCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTaaaa                                                              >  1:521528/1‑141 (MQ=255)
                                                                               ttCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATCTaaaa                                                              >  2:1227122/1‑141 (MQ=255)
                                                                               ttCATAACTTGTTACTAACATACTATTAAAATTACACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTaaaa                                                              <  1:739130/141‑1 (MQ=255)
                                                                                tCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTaaaaa                                                             <  1:40016/141‑1 (MQ=255)
                                                                                  aTACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATc                                                           <  1:1539304/141‑1 (MQ=255)
                                                                                  aTACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATc                                                           <  2:1213302/141‑1 (MQ=255)
                                                                                    aCCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCgg                                                         >  1:9986/1‑141 (MQ=255)
                                                                                      cTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGt                                                       >  1:1320090/1‑141 (MQ=255)
                                                                                       ttGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCATTGTTAAACat                                                                                                                                          <  2:784429/58‑2 (MQ=25)
                                                                                       ttGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCATTGTTAAACat                                                                                                                                          >  1:784429/1‑57 (MQ=25)
                                                                                       ttGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTg                                                      >  2:49658/1‑141 (MQ=255)
                                                                                        tGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGc                                                     >  1:1429382/1‑141 (MQ=255)
                                                                                         gTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTgg                                                     >  2:33054/1‑140 (MQ=255)
                                                                                         gTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCg                                                    <  1:983327/141‑1 (MQ=255)
                                                                                         gTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCg                                                    >  2:238748/1‑141 (MQ=255)
                                                                                          tCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCgg                                                   <  1:1078054/141‑1 (MQ=255)
                                                                                           cACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGa                                                  <  1:927683/141‑1 (MQ=255)
                                                                                           cACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGa                                                  <  2:738912/141‑1 (MQ=255)
                                                                                             cTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACg                                                >  1:1504130/1‑141 (MQ=255)
                                                                                               aaCATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGAt                                              <  1:300734/141‑1 (MQ=255)
                                                                                                     atatTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTACAAATCGGGTGCGGAAGATTTGGTc                                        >  1:1206126/1‑141 (MQ=255)
                                                                                                       atTAAAATTATACGAAAAACCGGTACTTTCAATGTTTGACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTa                                      >  1:1196920/1‑141 (MQ=255)
                                                                                                       atTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTa                                      >  2:287709/1‑141 (MQ=255)
                                                                                                       atTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTa                                      >  1:539466/1‑141 (MQ=255)
                                                                                                         tAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTAc                                                                                                                                 <  1:282330/49‑1 (MQ=255)
                                                                                                         tAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGa                                    >  1:363374/1‑141 (MQ=255)
                                                                                                           aaaTTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATa                                  >  2:279772/1‑141 (MQ=255)
                                                                                                            aaTTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATaa                                 <  2:159743/141‑1 (MQ=255)
                                                                                                             aTTATACGAAAAACCGGTACTTTCAATGTTTAACTATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAg                                <  1:1533904/141‑1 (MQ=255)
                                                                                                             aTTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAg                                >  2:674704/1‑141 (MQ=255)
                                                                                                             aTTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAg                                <  1:1414748/141‑1 (MQ=255)
                                                                                                             aTTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGGGCGGACGATTTGGTCTAGATAAg                                >  1:1584040/1‑141 (MQ=255)
                                                                                                              ttATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGa                               <  2:264733/141‑1 (MQ=255)
                                                                                                              ttATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGa                               <  1:1633921/141‑1 (MQ=255)
                                                                                                              ttATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGa                               <  1:1481094/141‑1 (MQ=255)
                                                                                                                ataCGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAAt                             >  2:530249/1‑141 (MQ=255)
                                                                                                                   cGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCt                          >  2:422233/1‑141 (MQ=255)
                                                                                                                        aaCCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTg                     >  1:1132972/1‑141 (MQ=255)
                                                                                                                          ccGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGa                   <  1:1050283/141‑1 (MQ=255)
                                                                                                                             gTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAAtt                >  2:309533/1‑141 (MQ=255)
                                                                                                                              tACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAATTg               <  1:1075810/141‑1 (MQ=255)
                                                                                                                                   tCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAATTGACAAg          >  1:1316288/1‑141 (MQ=255)
                                                                                                                                    cattGTTAAACATTGAAAGAACAGGTTTTAGTAGCAAATGTATATATATTGTTGAGATTATAATGAAGCTTCAATAAATGACTTAGAAAGAGGGTGCGGCAGATTTGGTGTAGATAAGAATGCTGGCAGGAATGGACAAga         >  2:1631271/4‑140 (MQ=255)
                                                                                                                                    cAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTgg                    <  1:392239/130‑1 (MQ=255)
                                                                                                                                    cAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAATTGACAAGc         <  2:1497413/141‑1 (MQ=255)
                                                                                                                                    cAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAATTGACAAGc         <  2:490438/141‑1 (MQ=255)
                                                                                                                                    cAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAATTGACAAGc         >  2:169737/1‑141 (MQ=255)
                                                                                                                                          ttAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAATTGACAAGCAATaaa   >  1:263646/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                             
AATTATGTATACTATGACATTTATACACAAATGATTGAAATGACAAGAGACTTAGAATTATAAAAGTCACTATTGTTATTTCATACCTTGTCACTAACATAATATTAAAATTATACGAAAAACCGGTACTTTCAATGTTTAACAATGAAAGTACCGGTTTTATTAGCAAATTTATTTATTTTGTTGATATTTTAATGAAGCTTCAATAAATGATTTAAAAATCGGGTGCGGACGATTTGGTCTAGATAAGAATTCTGGGTGGAATTGACAAGCAATAAAG  >  CP000730/2243360‑2243639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: