Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,504,989 T→C 7.7% intergenic (+293/+111) USA300HOU_2365 → / ← gltT hypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,504,9890TC 7.7% 477.1 / 8.2 197intergenic (+293/+111)USA300HOU_2365/gltThypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter
Reads supporting (aligned to +/- strand):  ref base T (105/72);  new base C (9/9);  total (116/81)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

TTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCTTT  >  CP000730/2504849‑2505129
                                                                                                                                            |                                                                                                                                             
tttGCGCATGCATAAAAGCCCCTAACAACCTGCGGTCACCACGCTCCGGCTCGCCCGCGCTTTAGCGCATGCATACAAGCCCCTAACAACCTGACGTCACTACGCGACCGCTCGCCTGCGCTTTAGCGCACGCATAAAAGt                                                                                                                                               >  2:375150/1‑141 (MQ=11)
  tGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAGAGGTCACTACGCGTCGGTTCGCCTGGGCTGTAGCGAATGCATAAAAGAcc                                                                                                                                             >  2:1382213/1‑141 (MQ=14)
   gcgcATGCATAAAAGCCCCTAACAACCCGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTccc                                                                                                                                            <  1:939201/141‑1 (MQ=255)
    cgcATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCt                                                                                                                                           >  1:1606286/1‑141 (MQ=255)
     gcatgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTa                                                                                                                                          <  1:1554740/141‑1 (MQ=255)
     gcatgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTa                                                                                                                                          <  1:1111271/141‑1 (MQ=255)
      catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaa                                                                                                                                         <  1:1016641/141‑1 (MQ=255)
      catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaa                                                                                                                                         >  2:1301554/1‑141 (MQ=12)
      catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAGAGGTCGCTACGCTTCGGATCGCCCACGCAGTAGCGCATGAATAAATGCACCTaa                                                                                                                                         >  2:1128270/1‑141 (MQ=14)
       atgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCttac                                                                                                                                        <  2:606562/141‑4 (MQ=2)
         gcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGGTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                                                                                      >  2:1425244/1‑141 (MQ=255)
         gcatAAAAGCCACTAACATCCTGAGGTCACTGCACTCCGGTTCGCCTGCGCTTTAGCTGATGCATAAAAGCCCCTAACAACCAGAGGTCACTACGCTTCGTTTCGCCTGCGCTTTAGTGCATGCATACAAGTCCCTaacaa                                                                                                                                      >  1:204262/1‑141 (MQ=14)
           atAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAAcc                                                                                                                                    <  1:1192071/141‑1 (MQ=255)
              aaaGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGa                                                                                                                                 >  2:322415/1‑141 (MQ=255)
              aaaGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGa                                                                                                                                 >  2:237999/1‑141 (MQ=255)
              aaaGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGa                                                                                                                                 >  2:578177/1‑141 (MQ=255)
                     cTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTGCGGATCGCCTGCGCTTTAGCGCATGCATAAAAGTCACTAACAACCGGAGGTCACt                                                                                                                          >  1:598774/1‑141 (MQ=255)
                     cTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTCCGTTTCGCCTGCGCATTAGCGCATGCATAAAAGTCCCTAACAAccaaccgttgcc                                                                                                                          >  2:1458140/1‑131 (MQ=14)
                        acaagCTGGGGTCGCTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACg                                                                                                                       <  2:667082/141‑1 (MQ=255)
                        acaacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACCACg                                                                                                                       >  2:1600012/1‑141 (MQ=255)
                        acaacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACCACg                                                                                                                       >  2:1182454/1‑141 (MQ=255)
                          aacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCt                                                                                                                     >  1:826156/1‑141 (MQ=255)
                             cTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATGAAAGTCCCTAACAACCTGAGGTCACTACGCTTcc                                                                                                                  >  2:106110/1‑141 (MQ=255)
                             cTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTcc                                                                                                                  >  2:211148/1‑141 (MQ=255)
                              tGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCg                                                                                                                 >  1:290737/1‑141 (MQ=255)
                                  gTCGCTGCGCTCCGGTTCGGCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCg                                                                                                             <  2:979110/141‑1 (MQ=255)
                                  gTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCg                                                                                                             <  2:1585606/141‑1 (MQ=255)
                                    cACTGCGCTCGGGTTCGCCTGCCCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGcc                                                                                                           <  2:740145/141‑1 (MQ=255)
                                    cACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCAGAGGTCAGAACGCtcatttacgct                                                                                                           >  2:1489919/1‑131 (MQ=14)
                                      cTGCGCTCCGGTTCGCCTGCGCTTTAGCGCCTGCATAAAAGCCGCTAACAACCTGAGGCCACCACGCTTTGTTTAGCCTCCGCTTTAGCGCATGGATAAAAGTCTCTAACAACCTAGCGTCGTGACGCGACCGTTCGccag                                                                                                         >  2:1200353/1‑139 (MQ=9)
                                       tGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCCGAGGTCACTACGCTCCCGTTCGCCTgc                                                                                                        >  2:913793/1‑141 (MQ=255)
                                        gcgcTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgcg                                                                                                       >  1:586828/1‑141 (MQ=255)
                                         cgcTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCATgcgc                                                                                                      <  1:719373/141‑1 (MQ=255)
                                          gcTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCCGAGGTCACCACGCCCCCGCTCGcccccgcc                                                                                                     >  2:526528/1‑135 (MQ=17)
                                            tCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCttt                                                                                                   >  1:775217/1‑141 (MQ=255)
                                              cGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGGGCTTTAGCGCATGCATAGAAGTCCCTAACAACCTGCGGTCACTACGGTTCTGTTCGctttcccattag                                                                                                 >  2:1063504/1‑130 (MQ=14)
                                               ggTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAgc                                                                                                <  1:1525291/141‑1 (MQ=255)
                                                gTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCAGACGTCACTACGCTTCCGTTCGCCTGCGCTTTAgcg                                                                                               >  1:1193979/1‑141 (MQ=255)
                                                 ttCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGGCACTACGCTGCGGTTCGCCTGCGGTTGAGCTCATGCATAGAAGGCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAgcgc                                                                                              >  1:1402740/1‑141 (MQ=14)
                                                  tCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgca                                                                                             >  2:81494/1‑141 (MQ=255)
                                                   cGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcat                                                                                            >  1:1040915/1‑141 (MQ=255)
                                                     ccTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCTTTCGCCTGCACTGTATCgcatgc                                                                                          >  2:1233302/1‑141 (MQ=255)
                                                     ccTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTCCCGATCGCCCGCGCAGTAGCgcatgc                                                                                          >  2:758382/1‑141 (MQ=14)
                                                      cttcgcTGTTGCGCATGCATAAAAGCCCCCAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgca                                                                                         <  2:293482/138‑1 (MQ=11)
                                                      cTGTGCTTTTGCGGATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgca                                                                                         <  1:300626/141‑1 (MQ=255)
                                                       ctccccttcccccatgcctAACAGCCCCTTACCACCTGAAGTCACTCCACATCGCTTCGCCCGCGCTTTAGCGCATGCATAACAGCCCCTACCAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgcat                                                                                        <  2:1450684/129‑1 (MQ=11)
                                                          gcTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATaaa                                                                                     <  1:322415/141‑1 (MQ=255)
                                                            tttAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAg                                                                                   <  1:133847/141‑1 (MQ=255)
                                                             aaaGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGc                                                                                  >  2:1503362/3‑141 (MQ=255)
                                                              tAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAGGcc                                                                                 <  1:1474812/141‑1 (MQ=255)
                                                              tAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGcc                                                                                 >  1:659274/1‑141 (MQ=255)
                                                                gcgcATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTCCCGTTCGCCTGCGCATTAGCGCATACATAAAATcccc                                                                               >  2:770431/1‑141 (MQ=255)
                                                                    atgcatAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaac                                                                           <  1:237999/141‑1 (MQ=255)
                                                                      ccatacactccccTCACACCCTTATTTCACCACACTTCCCTTCCCCTGCCCTTTACCGCATCCATAAAACTCCCTAACAACCTCAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                         <  2:1531744/132‑1 (MQ=9)
                                                                         tAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCGCCCGTTCGCCTGCGCATTAGCGCATGCATAAAAGCCCCTAACAACCa                                                                     >  2:696554/1‑141 (MQ=255)
                                                                            aaGCCCCTAACCACCTGAGGTCACTCCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAg                                                                  <  2:189317/141‑1 (MQ=255)
                                                                            aaGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTTACAAcctttg                                                                   <  1:105487/141‑5 (MQ=255)
                                                                             aGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTTACAAcctttgg                                                                  <  2:826156/141‑6 (MQ=255)
                                                                              gCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATACATGAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGt                                                                >  1:30718/1‑141 (MQ=255)
                                                                              gCCCCCTACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGt                                                                <  1:1278125/141‑1 (MQ=255)
                                                                                   tAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTaa                                                           >  1:862871/1‑141 (MQ=255)
                                                                                   tAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTGCCGTTAGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAAGGTTGTa                                                            >  2:828186/1‑141 (MQ=255)
                                                                                     acaacCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAgg                                                         <  1:526528/141‑1 (MQ=255)
                                                                                      cagccTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAGAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTTACAACCTTTGGTTGTTAggg                                                        <  1:1138145/138‑1 (MQ=14)
                                                                                         ccTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCt                                                     >  2:433239/1‑141 (MQ=255)
                                                                                         ccTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCt                                                     <  1:352135/141‑1 (MQ=255)
                                                                                          cTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCtt                                                    >  1:1170931/1‑141 (MQ=255)
                                                                                          cTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCATTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCtt                                                    >  2:1103037/1‑141 (MQ=255)
                                                                                           tGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCttt                                                   >  1:1622352/1‑141 (MQ=255)
                                                                                              ggTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaac                                                                                                                                        >  1:499870/1‑54 (MQ=255)
                                                                                               gTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAAt                                               >  1:613288/1‑141 (MQ=255)
                                                                                                 cACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGt                                             <  1:1382227/141‑1 (MQ=255)
                                                                                                     aCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAACGGTTGTAAGGGTCTTTAAATGTCAGa                                         >  2:1007462/1‑141 (MQ=255)
                                                                                                      cGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGAt                                        <  1:953105/141‑1 (MQ=255)
                                                                                                      cGCTTCGGTTCGCCTGCGCTTTAGCGCAGGCATAAAAGTCCCGAACAACCTGAGGTCACTACGCGTCCGTTCGCCCGCGCATTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGAt                                        >  2:1653885/1‑141 (MQ=18)
                                                                                                       gCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATa                                       >  1:1210475/1‑141 (MQ=255)
                                                                                                        cTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAg                                      >  1:150050/1‑141 (MQ=255)
                                                                                                         ttCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTa                                                                                                  >  1:448630/1‑81 (MQ=255)
                                                                                                           cGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAt                                   >  2:1392295/1‑141 (MQ=255)
                                                                                                           cGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAt                                   <  1:732190/141‑1 (MQ=255)
                                                                                                             gTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAttt                                 <  1:1146129/141‑1 (MQ=255)
                                                                                                                cGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAtttttt                              >  2:1624894/1‑141 (MQ=255)
                                                                                                                  ccTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTaa                            >  2:104642/1‑141 (MQ=255)
                                                                                                                   cTGCGCTTTAGCGCATGCATGAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTaaa                           <  1:106110/141‑1 (MQ=255)
                                                                                                                   cTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTCCCGTTCGCCTGCGCTCTAGCGCACGCATAAAAGCCCCTAACACCCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTaaa                           >  1:744046/1‑141 (MQ=255)
                                                                                                                    tGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAAt                          <  1:1648799/141‑1 (MQ=255)
                                                                                                                      cgcTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTg                        >  2:286220/1‑141 (MQ=255)
                                                                                                                          ttAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGAttt                    <  2:35808/141‑1 (MQ=255)
                                                                                                                            aGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTCCGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGGTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTa                  >  2:1530577/1‑141 (MQ=255)
                                                                                                                            aGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTa                  >  2:449383/1‑141 (MQ=255)
                                                                                                                             gcgcATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTaa                 >  2:1116305/1‑141 (MQ=255)
                                                                                                                                catgcatAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAata              >  1:1391395/1‑141 (MQ=255)
                                                                                                                                  tgcatAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATAtt            >  1:758730/1‑141 (MQ=255)
                                                                                                                                    catAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCt          <  1:1538983/141‑1 (MQ=255)
                                                                                                                                     ctAAAAGCCCCTAACAACCTGGGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCtt         <  1:168319/140‑1 (MQ=255)
                                                                                                                                     atAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCtt         >  2:1269312/1‑141 (MQ=255)
                                                                                                                                      tAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTg        >  1:1375991/1‑141 (MQ=255)
                                                                                                                                        aaaGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGAc      >  1:1439099/1‑141 (MQ=255)
                                                                                                                                         aaGTCCCTAACAACCTGAGGTCAATACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGAcc     >  1:387049/1‑141 (MQ=255)
                                                                                                                                          aGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCt    <  2:899029/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCTTT  >  CP000730/2504849‑2505129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: