Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,517,782 T→A 5.2% L950L (CTA→CTT narG ← nitrate reductase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,517,7820TA 5.2% 517.9 / 14.4 154L950L (CTA→CTTnarGnitrate reductase alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (76/70);  new base A (2/6);  total (78/76)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

Only 100 of 156 total aligned reads displayed.

GGTTGTGAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACTTACACCAT  >  CP000730/2517642‑2517921
                                                                                                                                            |                                                                                                                                           
ggTTGTGAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACtt                                                                                                                                             >  1:1407623/1‑141 (MQ=255)
 gTTGTGAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTa                                                                                                                                            >  1:1632734/1‑141 (MQ=255)
 gTTGTGAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTa                                                                                                                                            >  1:1648421/1‑141 (MQ=255)
 gTTGTGAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTa                                                                                                                                            >  1:694807/1‑141 (MQ=255)
 gTTGTGAAGTTATATTTAAAAACGAAAATTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTATGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTa                                                                                                                                            >  2:348399/1‑141 (MQ=255)
  ttGTGAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAg                                                                                                                                           >  2:994782/1‑141 (MQ=255)
  ttGTGAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAg                                                                                                                                           <  1:647227/141‑1 (MQ=255)
   tgtgAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGt                                                                                                                                          >  2:593143/1‑141 (MQ=255)
   tgtgAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGt                                                                                                                                          <  2:101960/141‑1 (MQ=255)
       aaGTTATATTTAAAAACGAAAATTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTAttt                                                                                                                                      <  2:542445/141‑2 (MQ=255)
        aGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTAtttc                                                                                                                                     <  2:125968/141‑3 (MQ=255)
        aGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGc                                                                                                                                     >  1:1017352/1‑141 (MQ=255)
         gTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCa                                                                                                                                    <  1:1553275/141‑1 (MQ=255)
             tatTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTCACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAg                                                                                                                                <  1:879906/141‑1 (MQ=255)
              atTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATctgc                                                                                                                               <  1:515430/141‑4 (MQ=255)
              atTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGc                                                                                                                               <  1:1524773/141‑1 (MQ=255)
                 tAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTAc                                                                                                                            <  2:1413319/141‑1 (MQ=255)
                  aaaaaCGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACa                                                                                                                           <  2:1578677/141‑1 (MQ=255)
                   aaaaCGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAt                                                                                                                          >  2:1495416/1‑141 (MQ=255)
                   aaaaCGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTAGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAt                                                                                                                          >  2:1316172/1‑141 (MQ=255)
                    aaaCGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt                                                                                                                         <  1:1171653/141‑1 (MQ=255)
                     aaCGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTa                                                                                                                        <  1:1505836/141‑1 (MQ=255)
                       cGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACg                                                                                                                      >  2:1076389/1‑141 (MQ=255)
                        gAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGt                                                                                                                     >  2:1265831/1‑141 (MQ=255)
                        gAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGt                                                                                                                     >  2:1430530/1‑141 (MQ=255)
                        gAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGt                                                                                                                     >  1:250805/1‑141 (MQ=255)
                         aaaTTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTg                                                                                                                    <  2:837470/141‑1 (MQ=255)
                                 tcAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCa                                                                                                            >  1:274767/1‑141 (MQ=255)
                                 tcAGCAGCGCGATCACTAGAAATATATTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCa                                                                                                            <  2:745767/141‑1 (MQ=255)
                                       gcgcGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGt                                                                                                      <  1:1615669/141‑1 (MQ=255)
                                       gcgcGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGt                                                                                                      >  1:801203/1‑141 (MQ=255)
                                          cgTTCGCTAGAAATATCTTGGAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGa                                                                                                   >  2:758792/1‑141 (MQ=255)
                                             tCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGa                                                                                                >  2:1051286/1‑141 (MQ=255)
                                              cGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAt                                                                                               <  1:1595122/141‑1 (MQ=255)
                                              cGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAt                                                                                               <  1:1264621/141‑1 (MQ=255)
                                              cGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAt                                                                                               <  2:879722/141‑1 (MQ=255)
                                               gCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAtt                                                                                              <  2:657799/141‑1 (MQ=255)
                                                   gAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGct                                                                                          <  2:1407623/141‑1 (MQ=255)
                                                        atCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTGCCATTCGTAGCAGATGAAATACTTAGTAGTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTcac                                                                                     <  1:213594/141‑1 (MQ=255)
                                                        atCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTcac                                                                                     <  2:1169991/141‑1 (MQ=255)
                                                          cTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGATATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAg                                                                                   >  2:867323/1‑141 (MQ=255)
                                                          cTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAg                                                                                   >  1:1554925/1‑141 (MQ=255)
                                                          cTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAg                                                                                   >  2:1625906/1‑141 (MQ=255)
                                                           ttttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGa                                                                                  <  1:25646/141‑1 (MQ=255)
                                                              tAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTTTTTTTTTTTTTTATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCTATCCGTGGACGATTCTCTTTCACAGAatc                                                                               >  2:1552433/1‑141 (MQ=255)
                                                               aaCGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatca                                                                              <  1:382026/141‑1 (MQ=255)
                                                               aaCGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatca                                                                              <  1:639577/141‑1 (MQ=255)
                                                                aCGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatcat                                                                             >  2:1445739/1‑141 (MQ=255)
                                                                    cATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCAtt                                                                         >  1:239516/1‑141 (MQ=255)
                                                                     aTTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTTTGTTATTTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCAttt                                                                        >  2:1521131/1‑141 (MQ=255)
                                                                     aTTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCAttt                                                                        <  1:345459/141‑1 (MQ=255)
                                                                     aTTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGATCTCACAGAATCATCAttt                                                                        >  2:1521040/1‑141 (MQ=255)
                                                                      ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTg                                                                       <  2:413576/141‑1 (MQ=255)
                                                                        ccAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTa                                                                     <  2:1553413/141‑1 (MQ=255)
                                                                        ccAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTa                                                                     >  1:373724/1‑141 (MQ=255)
                                                                         cAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTAt                                                                    >  2:651921/1‑141 (MQ=255)
                                                                           gTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCa                                                                  >  1:837881/1‑141 (MQ=255)
                                                                           gTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCa                                                                  >  1:80768/1‑141 (MQ=255)
                                                                           gTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCa                                                                  >  1:272163/1‑141 (MQ=255)
                                                                           gTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCa                                                                  >  2:601006/1‑141 (MQ=255)
                                                                           gTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCa                                                                  >  1:1480787/1‑141 (MQ=255)
                                                                            tttGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCAc                                                                 <  2:273727/141‑1 (MQ=255)
                                                                            tttGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCAc                                                                 <  1:567841/141‑1 (MQ=255)
                                                                                ttcttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTGGTATTGCATCAGCTGCAGTTCGGGCTGTAGCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCa                                                             >  1:746336/1‑141 (MQ=255)
                                                                                       agaTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt                                                      <  2:1211932/141‑1 (MQ=255)
                                                                                       agaTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt                                                      <  2:1644931/141‑1 (MQ=255)
                                                                                           cTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACa                                                  >  1:1054401/1‑141 (MQ=255)
                                                                                            ttCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACAt                                                 <  2:1472319/141‑1 (MQ=255)
                                                                                            ttCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACAt                                                 >  1:1599797/1‑141 (MQ=255)
                                                                                            ttCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACAt                                                 >  1:1494713/1‑141 (MQ=255)
                                                                                            ttCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACAt                                                 >  2:405185/1‑141 (MQ=255)
                                                                                             tCATATGATTTTTTTTTTTTTTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATa                                                >  2:290111/1‑141 (MQ=255)
                                                                                               atatGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACt                                              >  2:346247/1‑141 (MQ=255)
                                                                                                tatGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACtt                                             <  2:1459633/141‑1 (MQ=255)
                                                                                                 atGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACttt                                            >  2:1452783/1‑141 (MQ=255)
                                                                                                   gATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACttttt                                          <  1:1076389/141‑1 (MQ=255)
                                                                                                    aTTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTa                                         <  2:165717/141‑1 (MQ=255)
                                                                                                       tttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAAtt                                      >  2:1433638/1‑141 (MQ=255)
                                                                                                        ttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAAttc                                     >  1:314821/1‑141 (MQ=255)
                                                                                                         tgtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTCTTGCATCAGCTACCTTACGTCCTGTATAACTACGTGGCCGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAAttct                                    <  1:831013/141‑1 (MQ=255)
                                                                                                          gtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAAttctt                                   >  1:50088/1‑141 (MQ=255)
                                                                                                            gATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCa                                 >  1:1411776/1‑141 (MQ=255)
                                                                                                            actaccttACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCa                                 <  2:59998/135‑1 (MQ=255)
                                                                                                                     ccATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCa                        >  1:1055099/1‑141 (MQ=255)
                                                                                                                      cATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCAc                       >  1:771519/1‑141 (MQ=255)
                                                                                                                          cGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGac                   >  2:1004729/1‑141 (MQ=255)
                                                                                                                          cGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGac                   >  2:522801/1‑141 (MQ=255)
                                                                                                                          cGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGac                   >  1:586135/1‑141 (MQ=255)
                                                                                                                          cGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGac                   >  2:818646/1‑141 (MQ=255)
                                                                                                                            tAGCTGATGCAATACTAAGTATTTCATCTGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGacac                 >  1:11985/1‑141 (MQ=39)
                                                                                                                                  aTGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAAc           <  2:837881/141‑1 (MQ=255)
                                                                                                                                   tGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACt          <  1:1176708/141‑1 (MQ=255)
                                                                                                                                   tGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACt          <  1:1106065/141‑1 (MQ=255)
                                                                                                                                    gcaaTACTAAGTATTTCATCTGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACtt         >  1:1253584/3‑141 (MQ=255)
                                                                                                                                      aaTACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACTTac       >  1:1416855/1‑141 (MQ=255)
                                                                                                                                      aaTACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACTTac       >  1:1038021/1‑141 (MQ=255)
                                                                                                                                        ttcTTTGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACTTacac     <  1:348399/139‑1 (MQ=255)
                                                                                                                                        tACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACTTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACTTacac     >  2:1565920/1‑141 (MQ=255)
                                                                                                                                        tACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATCCTTTTTAATTCTTCATATTGTTCACTGACACCAAAACTTacac     >  2:723304/1‑141 (MQ=255)
                                                                                                                                        tACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACTTacac     <  2:794088/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GGTTGTGAAGTTATATTTAAAAACGAAATTTTCTCAGCAGCGCGTTCGCTAGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTTTTAATTCTTCATATTGTTCACTGACACCGAAACTTACACCAT  >  CP000730/2517642‑2517921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: