Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,608,825 A→T 10.6% intergenic (‑676/+154) USA300HOU_2470 ← / ← USA300HOU_2471 dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,608,8250AT10.6% 375.6 / 29.3 122intergenic (‑676/+154)USA300HOU_2470/USA300HOU_2471dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (48/61);  new base T (8/5);  total (56/66)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 130 total aligned reads displayed.

TAATTTTAAACAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGGTAGTAATCA  >  CP000730/2608686‑2608965
                                                                                                                                           |                                                                                                                                            
tAATTTTAAACAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAg                                                                                                                                             >  2:1356412/1‑141 (MQ=255)
  aTTTTAAACAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGa                                                                                                                                           <  2:1078185/141‑1 (MQ=255)
    tttAAACAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACGTCCTATATTGGatg                                                                                                                                         <  2:513535/141‑3 (MQ=255)
    tttAAACAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACGTCCTATATTGGatg                                                                                                                                         <  2:379363/141‑3 (MQ=255)
     ttAAACAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATTATCTACGTATGACGAAAGCCACGTCCTATATTGGatgt                                                                                                                                        <  2:909277/141‑4 (MQ=255)
        aaCAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGc                                                                                                                                     >  2:236031/1‑141 (MQ=255)
        aaCAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGc                                                                                                                                     <  1:26505/141‑1 (MQ=255)
            aGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCtttt                                                                                                                                 >  1:936893/1‑141 (MQ=255)
             gTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCGTTTa                                                                                                                                >  2:314540/1‑141 (MQ=255)
             gTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGAAGTGGCTTGTa                                                                                                                                >  2:61457/1‑141 (MQ=255)
              ttGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAg                                                                                                                               <  2:865352/141‑1 (MQ=255)
              ttGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACACCCAATATAGGACGTGGCTTTTAg                                                                                                                               <  1:1659462/141‑1 (MQ=255)
                     tGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGtt                                                                                                                        <  2:1520539/141‑1 (MQ=255)
                       aaGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTcc                                                                                                                      <  2:1308327/141‑1 (MQ=255)
                        aGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCa                                                                                                                     >  1:47263/1‑141 (MQ=255)
                        aGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTGAAAAATAATATAAGTATCACTAAAGCAACATCCAATATAGGACGTCGCTTGTAGTATCGATCCa                                                                                                                     >  2:788869/1‑141 (MQ=255)
                         ggATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAg                                                                                                                    <  2:533487/141‑1 (MQ=255)
                               tAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTtgt                                                                                                              <  2:1289733/141‑1 (MQ=255)
                                aaTATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTtgtg                                                                                                             <  1:503945/141‑1 (MQ=255)
                                aaTATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTtgtg                                                                                                             <  1:1048712/141‑1 (MQ=255)
                                 atatCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTgg                                                                                                            <  1:321991/141‑1 (MQ=255)
                                  tatCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTtgtaaa                                                                                                           >  1:97198/1‑138 (MQ=255)
                                  tatCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTtgtaaa                                                                                                           >  1:1350497/1‑138 (MQ=255)
                                   atCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTgggg                                                                                                          >  2:1095363/1‑141 (MQ=255)
                                     cATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAg                                                                                                        >  1:644104/1‑141 (MQ=255)
                                      aTTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGt                                                                                                       <  2:1587020/141‑1 (MQ=255)
                                          cGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAgg                                                                                                   >  1:73037/1‑141 (MQ=255)
                                          cGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAgg                                                                                                   >  1:72922/1‑141 (MQ=255)
                                           ttttATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAggg                                                                                                  <  1:1340721/140‑1 (MQ=255)
                                           gTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAggg                                                                                                  <  1:91878/141‑1 (MQ=255)
                                           gTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAggg                                                                                                  <  2:828245/141‑1 (MQ=255)
                                           gTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAggg                                                                                                  <  1:720805/141‑1 (MQ=255)
                                           gTTTATATATAGCAAACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAggg                                                                                                  <  1:117865/141‑1 (MQ=255)
                                             ttATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGtt                                                                                                <  2:1551515/141‑1 (MQ=255)
                                             ttATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGtt                                                                                                >  1:515177/1‑141 (MQ=255)
                                                 atataGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACt                                                                                            <  2:974570/141‑1 (MQ=255)
                                                  tataGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACtt                                                                                           <  2:463448/141‑1 (MQ=255)
                                                    taGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACttaa                                                                                         >  1:329488/1‑141 (MQ=255)
                                                     aGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACttaat                                                                                        <  1:1099544/141‑1 (MQ=255)
                                                       cAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACttaatta                                                                                      <  1:559589/141‑1 (MQ=255)
                                                        aGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATGGTTCCAGATTTGTGGGGAGTTAGGGTTTACttaattca                                                                                     >  2:645822/1‑139 (MQ=255)
                                                          aCATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAg                                                                                   >  1:195129/1‑141 (MQ=255)
                                                           cATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAgg                                                                                  >  2:260700/1‑141 (MQ=255)
                                                                 aGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTg                                                                            >  1:1315967/1‑141 (MQ=255)
                                                                     ttttATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGtt                                                                                                                        >  1:1372230/1‑93 (MQ=255)
                                                                     ttttATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCt                                                                        <  1:1266263/141‑1 (MQ=255)
                                                                      tttATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTc                                                                       <  1:519918/141‑1 (MQ=255)
                                                                       ttATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCa                                                                      <  1:1469393/141‑1 (MQ=255)
                                                                        tataTGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCaa                                                                     <  1:690210/141‑1 (MQ=255)
                                                                         atatGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAGGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGCTTTGTGGGGAGTTAGGGTTTAATTAATTACAGCGGTCTGCCCGCAAt                                                                    >  2:1423377/1‑141 (MQ=255)
                                                                         atatGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGGTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCact                                                                    >  1:749980/1‑139 (MQ=255)
                                                                           atGTAAATCTTGTAGGTAAACGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTg                                                                  <  1:89796/141‑1 (MQ=255)
                                                                             gTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCt                                                                <  2:1528702/141‑1 (MQ=255)
                                                                              tAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTc                                                               <  1:1115971/141‑1 (MQ=255)
                                                                              tAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCAAATCGAATTTAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTc                                                               >  1:380420/1‑141 (MQ=255)
                                                                               aaaTCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCa                                                              <  2:121065/141‑1 (MQ=255)
                                                                                  tCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCa                                                           >  2:322353/1‑141 (MQ=255)
                                                                                    ttGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTcgttggtgagcaatt                                                         <  1:500374/141‑15 (MQ=255)
                                                                                      gTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGa                                                       >  1:486249/1‑141 (MQ=255)
                                                                                      gTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGa                                                       <  2:1229744/141‑1 (MQ=255)
                                                                                      gTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGATTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGa                                                       >  1:852390/1‑141 (MQ=255)
                                                                                        aGGTAATCCTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGAACATGGCTTTTAGTATCGTTCCAGATTTGTTGGGCGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAgg                                                     <  1:991715/141‑1 (MQ=255)
                                                                                             aTCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAg                                                >  1:234491/1‑141 (MQ=255)
                                                                                               cGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTa                                                                                                     <  2:59983/86‑1 (MQ=255)
                                                                                                gTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTa                                             >  1:1043343/1‑141 (MQ=255)
                                                                                                     aaaaaTAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTGAAGATTAGTGTGGATGTAAGGTTTTCTTAATTATAGGAGTCTTCCCTCAATTGCTCGCCAACGAGGGCAAGTTACgtca                                        >  1:1653313/1‑141 (MQ=255)
                                                                                                            atatAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCacac                                 <  2:966425/141‑1 (MQ=255)
                                                                                                             tataAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACt                                >  1:1006168/1‑141 (MQ=255)
                                                                                                             tataAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACt                                <  2:763880/141‑1 (MQ=255)
                                                                                                               taAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTc                              >  1:207753/1‑141 (MQ=255)
                                                                                                                  gTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAAt                           <  1:1054515/141‑1 (MQ=255)
                                                                                                                      gACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGGTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGCGGGCAAGTTACATCAATCACACTTCAATTgcc                       >  2:162417/1‑141 (MQ=255)
                                                                                                                       aCTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGt                                               >  2:1145074/1‑116 (MQ=255)
                                                                                                                       aCTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGt                                               <  1:1145074/116‑1 (MQ=255)
                                                                                                                       aCTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGt                                               >  2:1145132/1‑116 (MQ=255)
                                                                                                                       aCTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTgccg                      <  1:987661/141‑1 (MQ=255)
                                                                                                                       aCTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTgccg                      <  1:1268519/141‑1 (MQ=255)
                                                                                                                           aaGCCACATCCAATATAGGGCGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGa                  <  2:73037/141‑1 (MQ=255)
                                                                                                                           aaGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGa                  <  2:72922/141‑1 (MQ=255)
                                                                                                                            aGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGAc                 <  1:619214/141‑1 (MQ=255)
                                                                                                                               cacgtCCTATATTGGATGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAg              >  1:343596/5‑141 (MQ=255)
                                                                                                                                 caTCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAggg            >  2:1588546/1‑141 (MQ=255)
                                                                                                                                 caTCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAggg            >  2:776488/1‑141 (MQ=255)
                                                                                                                                 caTCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAggg            >  1:1511258/1‑141 (MQ=255)
                                                                                                                                  aTCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAgggg           >  1:1318533/1‑141 (MQ=255)
                                                                                                                                   tcctatatTGGATGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCa                                                              >  2:982579/5‑89 (MQ=255)
                                                                                                                                   tCCTATATTGGATGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCa                                                              <  1:982579/89‑1 (MQ=255)
                                                                                                                                    cctatatTGGATGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGgta         >  1:323735/4‑141 (MQ=255)
                                                                                                                                    ccAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGgta         <  1:86329/141‑1 (MQ=255)
                                                                                                                                     cAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGgtag        >  2:1027545/1‑141 (MQ=255)
                                                                                                                                       atatTGGATGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGgtagta      >  2:492946/1‑141 (MQ=255)
                                                                                                                                       atatAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGgtagta      <  1:809558/141‑1 (MQ=255)
                                                                                                                                        tatcGGACGTGGCTTTTAGTATCGTTCCAGATTTGAGGGGAGTTCGGGTTTACTTAAGTAAAGGGGTCTGCCCCCAATTGCTCCCCAAGGCGGGCAAGTTACATCAATCACACTTCAATTGCCGGCGACTAGGGGTAGTaa     >  2:870767/1‑141 (MQ=255)
                                                                                                                                         ataGGACGTGGCTTTTAGTATCGTTCCCGATTTGTGGGGCGTTCGGGTTTCCTTAATTAAAGGGGTCTTCCCTCAATTGCTCTCCGCCGCGGGCAAGTTACATCTATCGCACTTATATTGCCGCCGACTAGGGgtagttag    <  2:600960/141‑4 (MQ=255)
                                                                                                                                         ataGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCTATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGGTAGTAAt    <  1:976291/141‑1 (MQ=255)
                                                                                                                                         ataGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCTATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGGTAGTAAt    <  1:976245/141‑1 (MQ=255)
                                                                                                                                         ataGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGGTAGTAAt    >  1:144405/1‑141 (MQ=255)
                                                                                                                                           aGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAg                                                >  2:235804/1‑95 (MQ=255)
                                                                                                                                           aGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAg                                                <  1:235804/95‑1 (MQ=255)
                                                                                                                                           aGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGGTAGTAATCa  >  2:1095921/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TAATTTTAAACAAGTTGAAAATGAAGGATTCTAATATCATTTCGTTTATATATAGCAGACATGATAGAATTTTATATGTAAATCTTGTAGGTAATCGTTTTAAAAATAATATAAGTATGACTAAAGCCACATCCAATATAGGACGTGGCTTTTAGTATCGTTCCAGATTTGTGGGGAGTTAGGGTTTACTTAATTAAAGGGGTCTGCCCTCAATTGCTCACCAACGAGGGCAAGTTACATCAATCACACTTCAATTGCCGCCGACTAGGGGTAGTAATCA  >  CP000730/2608686‑2608965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: