Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,774,975 A→G 6.3% D737D (GAT→GAC clfB ← clumping factor B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,774,9750AG 6.3% 363.2 / 3.4 144D737D (GAT→GACclfBclumping factor B
Reads supporting (aligned to +/- strand):  ref base A (66/67);  new base G (7/3);  total (73/71)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

GAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATC  >  CP000730/2774835‑2775115
                                                                                                                                            |                                                                                                                                            
  atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                            <  1:1204322/141‑1 (MQ=255)
  atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                            >  2:841025/1‑141 (MQ=255)
   tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatct                                                                                                                                           >  1:182783/1‑141 (MQ=255)
     tgaatcACTGTCTGAATCCGACTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                                                                                         <  2:694744/141‑1 (MQ=255)
      gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                                                                                        >  1:140888/1‑141 (MQ=255)
           aCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                                                                                                   >  1:284709/1‑141 (MQ=255)
            cTGTCTGAATCCGAATCGCTGTCTGTATCTGAGTCGCTATCCGTGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                                  <  2:405381/141‑1 (MQ=14)
            cTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATTTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                                  >  1:439068/1‑141 (MQ=255)
             tGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGGCTGCGTGTGAATCGCTGGCTGAGTCCGGATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGt                                                                                                                                 >  2:1232616/1‑141 (MQ=14)
              gTCTGAATCCGAATCGCTATCTGATTCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                                                <  2:1346340/141‑1 (MQ=255)
                  gAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTa                                                                                                                            <  1:226125/141‑1 (MQ=255)
                   aaTCCGAATCGCTATCTGAATCTGAATCGCGATCCGAGTCGTAGGCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCCGAATCTCTATCTGAATCTGATTCGCTAGCTGATACTGTTTCTCTAGCTGTATCTCANTCGCTAt                                                                                                                           >  2:1242187/1‑141 (MQ=14)
                    atgtctttctgcctctgaatctgtaTCGCCATGTGAGTCTCGTTCGGTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCCGAATCTGAGTCGCTAtc                                                                                                                          <  2:248060/128‑1 (MQ=11)
                    aTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGGCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGACGCtgta                                                                                                                          >  2:570698/1‑138 (MQ=14)
                     tCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtct                                                                                                                         <  2:1005725/141‑1 (MQ=255)
                     tCCGAATCGCTATCTGAATCTAAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtct                                                                                                                         <  1:14271/141‑1 (MQ=255)
                      ctgAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGATTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctg                                                                                                                        <  2:1477373/139‑1 (MQ=255)
                        gAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCCGTCCGAGTCTGAATCCCTATCTGAATCCGAGtacctccctgag                                                                                                                      >  2:1318760/1‑130 (MQ=14)
                          aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtc                                                                                                                    <  2:1516591/141‑1 (MQ=255)
                          aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCCGAATCGCTTTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtc                                                                                                                    <  1:939250/141‑1 (MQ=255)
                            cGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGGTGtctgagtcag                                                                                                                  >  2:601479/1‑139 (MQ=255)
                            cGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctg                                                                                                                  <  1:901722/141‑1 (MQ=255)
                            cGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctg                                                                                                                  >  1:42305/1‑141 (MQ=255)
                               tATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagt                                                                                                               >  1:135595/1‑141 (MQ=255)
                                gtctgagtctgagTCGCTATCCGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                              >  2:1066345/2‑141 (MQ=11)
                                 tctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcg                                                                                                             >  1:1583949/1‑141 (MQ=255)
                                 tctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcg                                                                                                             >  1:532261/1‑141 (MQ=255)
                                  ctgaatctgaatCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgc                                                                                                            <  1:881267/141‑1 (MQ=255)
                                  ctgaatctgaatCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgc                                                                                                            <  1:510914/141‑1 (MQ=255)
                                  ctgaatctgaatCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgc                                                                                                            >  1:1561031/1‑141 (MQ=255)
                                  ctgaatctgaatCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCAGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgagtcgc                                                                                                            >  1:923185/1‑141 (MQ=255)
                                   tgaatctctgtctgtATCTGAGTCTGAATCGGTATCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCCGAATCTGAGTCGCTAtctgagtctgagtcgct                                                                                                           <  2:1213247/127‑1 (MQ=11)
                                    acgtctgccccGCTATCAGAGTCTGTGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGACTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctg                                                                                                          <  2:1647976/131‑1 (MQ=14)
                                      atctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTTAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtc                                                                                                        <  1:333853/141‑1 (MQ=255)
                                      atctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtc                                                                                                        <  1:1204906/141‑1 (MQ=255)
                                       tctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTAtctgagtctgagtcgctgtct                                                                                                       >  2:19415/1‑141 (MQ=255)
                                       tctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGATTCGCTATCTGAATCTGAGTCGCTGTCTGACTCGGAGCGGCTAACTGACTCCGAGTGGACGtctgactcttagacgctatct                                                                                                       >  2:1304945/1‑141 (MQ=9)
                                        ctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctg                                                                                                      <  1:1654335/141‑1 (MQ=255)
                                        ctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCACTAtctgagtctgagtcgctgtctg                                                                                                      >  2:886395/1‑141 (MQ=255)
                                          gattcGCTTTCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgaa                                                                                                    <  2:155010/138‑2 (MQ=255)
                                          gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                                                    >  2:1089724/1‑141 (MQ=255)
                                            atcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                                                                  >  1:1409826/1‑141 (MQ=255)
                                             tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtct                                                                                                 >  1:494094/1‑141 (MQ=255)
                                                      gAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgctg                                                                                        <  1:1388232/141‑1 (MQ=255)
                                                       aGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATctgagtctgagtcgctgtctgagtctgagtcgctgt                                                                                       >  1:56519/1‑141 (MQ=255)
                                                         tCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTgagtctgagtcgctgtctgagtctgagtcgctgtct                                                                                     >  1:71344/1‑141 (MQ=255)
                                                         tCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTgagtctgagtcgctgtctgagtctgagtcgctgtct                                                                                     <  2:305023/141‑1 (MQ=255)
                                                          cTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGagtctgagtcgctgtctgagtctgagtcgctgtctg                                                                                    <  2:1588730/141‑1 (MQ=255)
                                                           tGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAgtctgagtcgctgtctgagtctgagtcgctgtctga                                                                                   >  2:1513616/1‑141 (MQ=255)
                                                            gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                                  <  1:553357/141‑1 (MQ=255)
                                                              gTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                                <  1:1000054/141‑1 (MQ=255)
                                                                cGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGagtcgctgtctgagtctgagtcgctgtctgagtctg                                                                              <  1:804727/141‑1 (MQ=255)
                                                                    gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                          >  1:1263332/1‑141 (MQ=255)
                                                                     tctgaatctgaaTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCg                                                                         >  2:479414/1‑141 (MQ=255)
                                                                        gattctgagtgGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTa                                                                      <  2:1397446/138‑1 (MQ=255)
                                                                        gaatctgaatcGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTa                                                                      <  2:652436/141‑1 (MQ=255)
                                                                        gaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTa                                                                      <  1:528021/141‑1 (MQ=255)
                                                                          ctctggatcGCTCTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                    <  2:1343004/140‑1 (MQ=255)
                                                                          atctgctttgCTTTCTCAGTCTGATTCGCTATGTGTATCTGAGTCTGTGTCTGAGTCTGATTCGCTGTCTGTATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCtgtc                                                                    <  2:962531/132‑4 (MQ=11)
                                                                          atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGACTCTGAGTCGCTCTCTGAGTCTGACTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                    <  2:684853/141‑1 (MQ=255)
                                                                           tctgtatcGCTTTCTGATTCTGAATCGCTTTCTGATACTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatct                                                                   <  2:1027998/141‑1 (MQ=11)
                                                                            ctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTAGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg                                                                  >  1:321236/1‑141 (MQ=255)
                                                                              gaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                >  1:1105958/1‑141 (MQ=255)
                                                                                atcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                              <  1:1505620/141‑1 (MQ=255)
                                                                                 tcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTgagtcgctgtctgagtctgagtcgctgtctgagtctgagtcgctgtctgagtct                                                             >  2:741999/1‑141 (MQ=255)
                                                                                    cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                          >  1:608146/1‑141 (MQ=255)
                                                                                       tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                       >  1:15219/1‑141 (MQ=255)
                                                                                       tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                       >  2:466303/1‑141 (MQ=255)
                                                                                        cTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc                                                      >  1:1474313/1‑141 (MQ=255)
                                                                                          gAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGACTCTGAACCGCTATCTGATTCTGCGTCGCTg                                                    >  1:769642/1‑141 (MQ=255)
                                                                                          gAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGCATCTGAGTCGCTg                                                    >  2:1317568/1‑141 (MQ=255)
                                                                                             tCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTatct                                                 <  2:601987/141‑1 (MQ=11)
                                                                                                gAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                              <  1:496280/141‑1 (MQ=255)
                                                                                                  aTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTgtctgagtc                                            <  1:794727/141‑1 (MQ=255)
                                                                                                  aTCGCTATCTGAATCTGAGTCGCTGTCTGAGACTGAATCGCTATCTGAATCTGAGCCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgaatc                                            >  1:1367226/1‑141 (MQ=255)
                                                                                                      cTATCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCGGAGTCGCTGtctgagtctgag                                        >  2:97161/1‑141 (MQ=255)
                                                                                                      cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctgaa                                        >  2:1553181/1‑140 (MQ=255)
                                                                                                      cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                        >  1:1164713/1‑141 (MQ=255)
                                                                                                           tgaatctgaGTCGCTGTCTGAGTCCGAATCGCTATCTGATTCTGAGTCGCTATCTGAACCTGAGTCGCTGTCTGATTCTGACTCGCTGTCTGCGTCTGACTCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgct                                   <  2:661177/141‑1 (MQ=14)
                                                                                                             aatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgt                                 <  1:639330/141‑1 (MQ=255)
                                                                                                               tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTgagtctgagtcgctgtctgagtctgagtcgctgtct                               >  2:1035541/1‑141 (MQ=255)
                                                                                                               tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtct                               >  2:1251970/1‑141 (MQ=255)
                                                                                                                ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg                                                                  >  2:122258/1‑105 (MQ=255)
                                                                                                                ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg                                                                  <  1:122258/105‑1 (MQ=255)
                                                                                                                 tgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctg                                                                                                >  1:1276824/1‑74 (MQ=255)
                                                                                                                    gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGCCTGAGtctgagtcgctgtctgaatc                          >  1:1543387/1‑141 (MQ=255)
                                                                                                                      cGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                        <  2:1151827/141‑1 (MQ=255)
                                                                                                                      cGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                        <  2:1439299/141‑1 (MQ=255)
                                                                                                                        cTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag                      >  1:1521458/1‑141 (MQ=255)
                                                                                                                          ttctgagtctgagTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtc                    <  2:1457486/140‑1 (MQ=255)
                                                                                                                           tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGCGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                   >  2:918767/1‑141 (MQ=255)
                                                                                                                            ctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATtctgaatcgctatctgattctgagtcgc                  >  1:1027937/1‑141 (MQ=14)
                                                                                                                            ctgagtctgaATCGCTAGCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtcngagtcgctgtctgaatctgagtcgc                  <  1:570698/141‑1 (MQ=255)
                                                                                                                             tgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgct                 >  1:17716/1‑141 (MQ=255)
                                                                                                                                 tctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct             <  2:439068/141‑1 (MQ=255)
                                                                                                                                  ctgaGTCGCTATCTGAATCTGAGTCACTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctg            <  2:333459/141‑1 (MQ=255)
                                                                                                                                    gaATCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctgaa          <  2:428503/141‑1 (MQ=255)
                                                                                                                                      aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc        <  2:987637/141‑1 (MQ=255)
                                                                                                                                       tCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcg                                                                                                             <  1:1082910/39‑1 (MQ=40)
                                                                                                                                          cTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgaatctgaa    <  2:799309/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATC  >  CP000730/2774835‑2775115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: