Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,833,387 G→C 6.4% L70V (CTA→GTA)  USA300HOU_2672 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,833,3870GC 6.4% 423.6 / 19.5 125L70V (CTA→GTA) USA300HOU_2672hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (49/68);  new base C (2/6);  total (51/74)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

Only 100 of 126 total aligned reads displayed.

TGATGATTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATCTTTAACAGT  >  CP000730/2833247‑2833525
                                                                                                                                                 |                                                                                                                                          
tgatgaTTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAg                                                                                                                                            <  2:494975/141‑1 (MQ=255)
 gatgatTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGt                                                                                                                                           >  1:1057329/1‑141 (MQ=255)
  atgatTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGta                                                                                                                                          <  1:98091/141‑1 (MQ=255)
   tgatTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGtat                                                                                                                                         <  1:1038186/141‑1 (MQ=255)
   tgatTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGtat                                                                                                                                         <  1:91686/141‑1 (MQ=255)
   tgatTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATACtat                                                                                                                                         <  1:983841/141‑1 (MQ=255)
   tgatTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATACtat                                                                                                                                         <  1:262877/141‑1 (MQ=255)
   tgatTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATACtat                                                                                                                                         <  1:983802/141‑1 (MQ=255)
     atTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATaa                                                                                                                                       <  2:536210/141‑1 (MQ=255)
      ttATCATAAGAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTATTAATGGGTTATGACATTTCTATTTCAGGCTAGTTATATTTGTATT‑‑‑‑‑TATAGTATaaa                                                                                                                                      <  2:751551/141‑1 (MQ=255)
         gCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATAc                                                                                                                                   <  1:457003/141‑1 (MQ=255)
           aTAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATACTATAAATACaa                                                                                                                                 <  2:461046/141‑1 (MQ=255)
                  tACATCCCATATAGTTAGTTTTTTAGTCTAACTTTCGGGTTGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTATATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGt                                                                                                                          <  2:399604/141‑1 (MQ=255)
                   aCATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAATTAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTa                                                                                                                         >  1:490200/1‑141 (MQ=255)
                   aCATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTa                                                                                                                         >  1:1500496/1‑141 (MQ=255)
                    cATCCCATAAAGTTAGTTTTTTTTTTTTTTTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTaa                                                                                                                        >  2:959765/1‑141 (MQ=255)
                    cATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTaa                                                                                                                        <  1:967480/141‑1 (MQ=255)
                       cccATGAAGTTATTTTTTTCGTCTAACTTTCGGGATGCATATCTTAGTTTTATTGGAGATTGTCTTTTAAATAGGCTTTGACATTTCTATTTCATGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTCAAGa                                                                                                                     <  2:1303459/141‑1 (MQ=255)
                       cccATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGa                                                                                                                     <  1:91854/141‑1 (MQ=255)
                        ccATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGAc                                                                                                                    <  1:1607583/141‑1 (MQ=255)
                             aaGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTa                                                                                                               <  2:839035/141‑1 (MQ=255)
                              agttagttTTTTAGTCTCACTTTCGGGATGCATATCTTAGTTCTAGTGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTAt                                                                                                              <  1:1453504/141‑1 (MQ=255)
                              agttagttTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAATACAACTAt                                                                                                              >  2:1378750/1‑141 (MQ=255)
                               gttagttTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATg                                                                                                             <  1:765142/141‑1 (MQ=255)
                               gttagttTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATg                                                                                                             >  1:497860/1‑141 (MQ=255)
                                ttagttTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGa                                                                                                            <  2:358109/141‑1 (MQ=255)
                                 tagttTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGat                                                                                                           <  1:143145/141‑1 (MQ=255)
                                 tagttTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGat                                                                                                           >  2:1388457/1‑141 (MQ=255)
                                   gttTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTTACGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGatat                                                                                                         >  1:1621807/1‑141 (MQ=255)
                                   gttTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGatat                                                                                                         <  2:1190898/141‑1 (MQ=255)
                                         tAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAg                                                                                                   >  1:184093/1‑141 (MQ=255)
                                            tCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGgta                                                                                                <  2:681824/141‑1 (MQ=255)
                                             cTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGgtag                                                                                               >  1:512690/1‑141 (MQ=255)
                                               aaCTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGgtagta                                                                                             <  1:128799/141‑1 (MQ=255)
                                                  tttCTGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACg                                                                                          <  2:104805/141‑1 (MQ=255)
                                                     cGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACa                                                                                       >  1:498935/1‑141 (MQ=255)
                                                     cGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACa                                                                                       >  1:1418009/1‑141 (MQ=255)
                                                       ggATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACaaa                                                                                     >  1:1422448/1‑141 (MQ=255)
                                                        gATGCATATCTTAGTTCTACTGGAGATTGTCGTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAACGACAACTATGATATTAATAGGTAGTAACGACAAAc                                                                                    <  2:576763/141‑1 (MQ=255)
                                                        gATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAAc                                                                                    <  2:576695/141‑1 (MQ=255)
                                                              tatCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAAct                                                                              <  1:1016038/141‑1 (MQ=255)
                                                               atCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAActc                                                                             >  1:68005/1‑141 (MQ=255)
                                                               atCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAActc                                                                             >  1:1622035/1‑141 (MQ=255)
                                                               atCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAActc                                                                             <  2:822925/141‑1 (MQ=255)
                                                                  ttAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCttt                                                                          <  2:1062272/141‑1 (MQ=255)
                                                                      ttCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCt                                                                      >  1:427854/1‑141 (MQ=255)
                                                                       tCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCtt                                                                     >  2:834444/1‑141 (MQ=255)
                                                                           aTGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTc                                                                 >  1:1156932/1‑141 (MQ=255)
                                                                            tGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTGTT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCg                                                                <  2:1201488/141‑1 (MQ=255)
                                                                            tGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCg                                                                <  2:1165351/141‑1 (MQ=255)
                                                                            tGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCg                                                                <  1:1348666/141‑1 (MQ=255)
                                                                              gagaTTGTCTTTTAAATAGGGTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAAGGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGcc                                                              <  2:1139494/141‑1 (MQ=255)
                                                                              gagaTTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGcc                                                              <  1:1641476/141‑1 (MQ=255)
                                                                               agtttGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCa                                                             >  2:1026120/4‑141 (MQ=255)
                                                                               agaTTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATACTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCa                                                             <  1:466941/141‑1 (MQ=255)
                                                                                    gTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGGTATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAttttt                                                        >  2:177612/1‑141 (MQ=255)
                                                                                    gTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAttttt                                                        >  2:1284511/1‑141 (MQ=255)
                                                                                    gTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAttttt                                                        >  1:888064/1‑141 (MQ=255)
                                                                                     tCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAt                                                            >  2:1144879/1‑141 (MQ=255)
                                                                                       ttttAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTcc                                                     <  1:660170/141‑1 (MQ=255)
                                                                                          tAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCtt                                                  <  2:489364/141‑1 (MQ=255)
                                                                                             ctAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCAt                                               <  1:60032/140‑1 (MQ=255)
                                                                                             aTAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCAt                                               <  2:1459039/141‑1 (MQ=255)
                                                                                             aTAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCAt                                               <  1:852637/141‑1 (MQ=255)
                                                                                              tAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATa                                              >  2:435234/1‑141 (MQ=255)
                                                                                                ggCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TGTAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGGAGTAACGACAAACATAACTCTTTAGCTTTGTCGCCATTTTTTCCCTGCATAAc                                            <  2:58851/141‑1 (MQ=255)
                                                                                                 gCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAGTGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACt                                           <  2:1328152/141‑1 (MQ=255)
                                                                                                 gCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACt                                           <  2:1156932/141‑1 (MQ=255)
                                                                                                    aTGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTa                                                                         >  2:207626/1‑108 (MQ=255)
                                                                                                    aTGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTa                                                                         <  1:207626/108‑1 (MQ=255)
                                                                                                          tttCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATTATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTatat                                  >  2:620337/1‑141 (MQ=255)
                                                                                                               aTTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGAtt                             >  2:1278707/1‑141 (MQ=255)
                                                                                                                  tCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCat                          >  1:840751/1‑141 (MQ=255)
                                                                                                                   cAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCata                         <  2:935943/141‑1 (MQ=255)
                                                                                                                    aGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCatat                        <  1:435234/141‑1 (MQ=255)
                                                                                                                       cTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACt                     <  2:184093/141‑1 (MQ=255)
                                                                                                                         aGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAAc                                            <  1:1495500/116‑1 (MQ=255)
                                                                                                                         aGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAAc                                            >  2:1495500/1‑116 (MQ=255)
                                                                                                                         aGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACttt                   >  1:212935/1‑141 (MQ=255)
                                                                                                                         aGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACttt                   <  2:91736/141‑1 (MQ=255)
                                                                                                                         aGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACttt                   >  2:1033821/1‑141 (MQ=255)
                                                                                                                          gATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACtttt                  <  2:708232/141‑1 (MQ=255)
                                                                                                                           atatatTTGTATG‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAATACAACTATGATATTAATAGTGAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTc                 <  1:73133/141‑1 (MQ=255)
                                                                                                                             atatTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGa               <  2:99265/141‑1 (MQ=255)
                                                                                                                             atatTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGa               <  1:1179571/141‑1 (MQ=255)
                                                                                                                             atatTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGa               <  1:1415799/141‑1 (MQ=255)
                                                                                                                              tatTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGAc              >  2:154739/1‑141 (MQ=255)
                                                                                                                               atTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACa             <  1:315820/141‑1 (MQ=255)
                                                                                                                               atTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACa             <  1:315813/141‑1 (MQ=255)
                                                                                                                               atTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACa             <  2:1565369/141‑1 (MQ=255)
                                                                                                                                tttGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACAt            >  1:64320/1‑141 (MQ=255)
                                                                                                                                tttGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACAt            >  1:955245/1‑141 (MQ=255)
                                                                                                                                tttGTATT‑‑‑‑‑TATACTATAAATACAAAGAATGTAAAGACTACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACAt            >  1:1193859/1‑141 (MQ=255)
                                                                                                                                 ttGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATc           <  1:789333/141‑1 (MQ=255)
                                                                                                                                   gTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATCtt         <  1:560790/141‑1 (MQ=255)
                                                                                                                                      tt‑‑‑‑‑tATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATCTTTat      <  1:1575211/141‑2 (MQ=255)
                                                                                                                                      tt‑‑‑‑‑tATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATCTTTaa      >  1:850387/1‑141 (MQ=255)
                                                                                                                                              ataGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATCTTTAACAg   >  1:1072306/1‑141 (MQ=255)
                                                                                                                                               taGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATCTTTAACAGt  >  2:503092/1‑141 (MQ=255)
                                                                                                                                               taGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATCTTTAACAGt  >  2:674300/1‑141 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
TGATGATTAGCATAAAAATACATCCCATAAAGTTAGTTTTTTAGTCTAACTTTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATT‑‑‑‑‑TATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTTCATAACTTCATTATATCGATTCATATACTTTTCGACATCTTTAACAGT  >  CP000730/2833247‑2833525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: