Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 803,528 C→T 6.5% intergenic (+36/+42) USA300HOU_0750 → / ← dtpT hypothetical protein/POT family proton (H+)‑dependent oligopeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730803,5280CT 6.5% 453.6 / 12.4 149intergenic (+36/+42)USA300HOU_0750/dtpThypothetical protein/POT family proton (H+)‑dependent oligopeptide transporter
Reads supporting (aligned to +/- strand):  ref base C (61/75);  new base T (5/5);  total (69/80)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 150 total aligned reads displayed.

AAAATGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAT  >  CP000730/803388‑803667
                                                                                                                                            |                                                                                                                                            
  aaTGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCa                                                                                                                                            >  2:144044/1‑141 (MQ=255)
  aaTGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCa                                                                                                                                            <  1:82675/141‑1 (MQ=255)
  aaTGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCa                                                                                                                                            >  1:938102/1‑141 (MQ=255)
  aaTGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAAAAAATAAAAAAACAGTAAGCCa                                                                                                                                            <  2:1361521/141‑1 (MQ=255)
  aaTGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCCATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCa                                                                                                                                            <  1:29585/141‑1 (MQ=255)
   aTGGTAATAAATGAAATTGATTTCTATAGAGCGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCAt                                                                                                                                           >  2:1159278/1‑141 (MQ=255)
    tGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCAtt                                                                                                                                          >  2:75949/1‑141 (MQ=255)
        aataaATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTaaa                                                                                                                                      <  2:1100533/141‑1 (MQ=255)
         ataaATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAAt                                                                                                                                     >  2:859837/1‑141 (MQ=255)
         ataaATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAAt                                                                                                                                     <  1:342677/141‑1 (MQ=255)
             attaaaTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACt                                                                                                                                 <  2:201326/138‑1 (MQ=255)
                 aaTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACt                                                                                                                             <  1:198659/141‑1 (MQ=255)
                 aaTTGATTTCTATAGAGTGAGACGAGAAAATATAGTTATAGCTGACTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTatt                                                                                                                             <  2:303502/141‑3 (MQ=255)
                    tGATTTCTATAGAGTGGTACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtt                                                                                                                         <  1:349054/141‑1 (MQ=255)
                     gatttgaagagagGGGGACGAGAAAATAAAGTTAGAGTTAGCAGTAATTGGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGTATTTTATAAAAAAACTTTATACTATTTAAATTACTTACTGttt                                                                                                                        >  2:829856/10‑141 (MQ=255)
                     gATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTACTGttt                                                                                                                        >  2:1427038/1‑141 (MQ=255)
                      aTTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtttt                                                                                                                       <  2:809437/141‑1 (MQ=255)
                        ttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTACTGtttttt                                                                                                                     <  1:1427038/141‑1 (MQ=255)
                               gagTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAg                                                                                                              <  1:1588844/141‑1 (MQ=255)
                                   gggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCca                                                                                                          >  2:617608/1‑141 (MQ=255)
                                    ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                         <  2:1612100/141‑1 (MQ=255)
                                    ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                         >  2:340016/1‑141 (MQ=255)
                                     gACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                        <  2:291290/141‑1 (MQ=255)
                                     gACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                        <  1:1641954/141‑1 (MQ=255)
                                      aCGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaaca                                                                                                       >  1:547537/1‑141 (MQ=255)
                                          gaAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAatat                                                                                                   <  2:1507884/141‑1 (MQ=255)
                                           aaaaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATAtt                                                                                                  <  1:856890/141‑1 (MQ=255)
                                           aaaaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATAtt                                                                                                  <  2:1112603/141‑1 (MQ=255)
                                             aaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTaa                                                                                                >  1:1219900/1‑141 (MQ=255)
                                             aaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTaa                                                                                                >  1:1311252/1‑141 (MQ=255)
                                              atatAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAAc                                                                                               >  1:618313/1‑141 (MQ=255)
                                                ataGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCAAACAATATTAACGt                                                                                             >  1:1510312/1‑141 (MQ=255)
                                                 taGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGta                                                                                            >  1:972408/1‑141 (MQ=255)
                                                 taGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGta                                                                                            >  1:972333/1‑141 (MQ=255)
                                                   gTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGtata                                                                                          >  2:471830/1‑141 (MQ=255)
                                                       taGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCtt                                                                                      <  1:5900/141‑1 (MQ=255)
                                                       taGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCtt                                                                                      <  2:1466863/141‑1 (MQ=255)
                                                         gCTGTCTATAATGAGCATATTAAGTTTTTTTTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTc                                                                                    >  2:1237456/1‑141 (MQ=255)
                                                         gCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTTAATTAGGCCAACAATCTTAACGTATACCTTTc                                                                                    >  2:1035780/1‑141 (MQ=255)
                                                          cTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCa                                                                                   <  1:248967/141‑1 (MQ=255)
                                                                  aaTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTCCTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtg                                                                           <  1:417685/141‑1 (MQ=255)
                                                                   aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtga                                                                          <  2:51238/141‑1 (MQ=255)
                                                                   aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAAAGTATACCTTTCATCGCTTtga                                                                          >  1:850420/1‑141 (MQ=255)
                                                                     gAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtgatg                                                                        <  2:207078/141‑1 (MQ=255)
                                                                      aGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTACTAGAACCCTATCATTACACTGTAAGCCAATTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtgatga                                                                       <  1:1653200/141‑1 (MQ=255)
                                                                       gCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTgatgat                                                                      <  1:1089207/141‑1 (MQ=255)
                                                                        cATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGAtt                                                                     >  2:794617/1‑141 (MQ=255)
                                                                            ttAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAg                                                                 <  2:1184422/141‑1 (MQ=255)
                                                                             tAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAgg                                                                <  2:285089/141‑1 (MQ=255)
                                                                             tAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAgg                                                                <  1:1470282/141‑1 (MQ=255)
                                                                             tAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAgg                                                                <  1:1470522/141‑1 (MQ=255)
                                                                               aGTATTTATTTATACTGATATTTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTg                                                              <  2:432232/141‑1 (MQ=255)
                                                                                gTTTTTATTTATACTGATATCTTGAATTTAATTACTAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTga                                                             <  1:1536249/141‑1 (MQ=255)
                                                                                gTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtga                                                                          >  2:757494/1‑128 (MQ=255)
                                                                                  ttttATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTgaga                                                           <  1:651571/141‑1 (MQ=255)
                                                                                     tatttttACTGTAATCTTAAAATTAATTAAGATAATCTTAGTAAAAAACAGTAAGGCATTTAATTTACTCACTGTTTTTTGAATTAGGCCAACAGTATTGGCGTATACCTTTTTTCTCTATGATGAGGAAATGCGTGATTg                                                        >  2:1184260/1‑141 (MQ=255)
                                                                                      atttatACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGc                                                       >  2:185396/1‑141 (MQ=255)
                                                                                       tttatACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCt                                                      <  1:75949/141‑1 (MQ=255)
                                                                                          ataCTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAAt                                                   >  1:671431/1‑141 (MQ=255)
                                                                                          ataCTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAAt                                                   <  1:883754/141‑1 (MQ=255)
                                                                                             cTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACa                                                >  1:1018498/1‑141 (MQ=255)
                                                                                              tGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACaa                                               >  2:196685/1‑141 (MQ=255)
                                                                                              tGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACaa                                               <  2:687635/141‑1 (MQ=255)
                                                                                               gATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAAt                                              >  1:1004816/1‑141 (MQ=255)
                                                                                                   tCTTGAATTTAATTAATAGAAACACCTAATGCAAATTTAGGGTATTTTAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAAttgtt                                          >  2:494329/1‑141 (MQ=255)
                                                                                                     ttGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATtgttgt                                        >  1:789131/1‑141 (MQ=255)
                                                                                                     ttGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGAAATCTAAATGACATACTGTATATTGAATTAGGCCAACAAAATTAACGTATACCTTTCATCGCTTTAATGATTAAAGGTAACATTGCTAATACAAGtgttgt                                        >  2:417534/1‑141 (MQ=255)
                                                                                                      tGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTa                                       >  2:491193/1‑141 (MQ=255)
                                                                                                       gactctaattattAGAAATCTGTAAAAAAACAGTAATCCATTTATATGACTTACTGCTTTTTGAATTAGGCCAAAAATATTAACGTATACCTTTCATCTCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTaa                                      >  2:849285/6‑141 (MQ=255)
                                                                                                       gAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTaa                                      >  2:278986/1‑141 (MQ=255)
                                                                                                        aaTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAAc                                     <  1:42722/141‑1 (MQ=255)
                                                                                                         aTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAAc                                     <  1:658024/141‑1 (MQ=255)
                                                                                                         aTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACa                                    <  2:587548/141‑1 (MQ=255)
                                                                                                         aTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACa                                    >  2:700582/1‑141 (MQ=255)
                                                                                                         aTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACa                                    >  1:474295/1‑141 (MQ=255)
                                                                                                            taattaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTATTGTTACaaca                                 <  1:544753/141‑3 (MQ=255)
                                                                                                             aattaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataa                                >  1:1049978/1‑141 (MQ=255)
                                                                                                             aattaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACATATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataa                                >  2:879555/1‑141 (MQ=255)
                                                                                                              attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                               >  2:1496034/1‑141 (MQ=255)
                                                                                                              attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                               >  1:1515734/1‑141 (MQ=255)
                                                                                                              attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                               >  2:1304467/1‑141 (MQ=255)
                                                                                                              attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                               >  1:372209/1‑141 (MQ=255)
                                                                                                                   tAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTCCTGTTTTTTGAATTAGGCCAACAATATTAACGTATCCCTTTCAGCGCTTTGATGATCAAAAGCGAGATTGCTAATACAATTTTTGTACCAATAATCGcaa                          >  2:292318/1‑141 (MQ=255)
                                                                                                                   tAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaa                          >  1:1612879/1‑141 (MQ=255)
                                                                                                                   aaGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaa                          <  1:1067073/140‑1 (MQ=255)
                                                                                                                      aaaCCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaacaa                       <  1:1047604/141‑1 (MQ=255)
                                                                                                                      aaaCCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaacaa                       <  1:1615887/141‑1 (MQ=255)
                                                                                                                        aCCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAacaaca                     <  2:404290/141‑1 (MQ=255)
                                                                                                                          cTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaacacttta                   >  1:1145978/1‑136 (MQ=255)
                                                                                                                           tataAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCt                  <  1:769130/141‑1 (MQ=255)
                                                                                                                            ataAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTa                 >  2:677390/1‑141 (MQ=255)
                                                                                                                             taAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAg                <  1:519340/141‑1 (MQ=255)
                                                                                                                                   aaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt          >  1:1317024/1‑141 (MQ=255)
                                                                                                                                    aCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                        >  2:1445340/1‑46 (MQ=255)
                                                                                                                                    aCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                        <  1:1445340/46‑1 (MQ=255)
                                                                                                                                       gTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg      >  1:703822/1‑141 (MQ=255)
                                                                                                                                        tAAGTCATTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTa                                       >  2:1224260/1‑107 (MQ=255)
                                                                                                                                        tAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGt     <  2:524283/141‑1 (MQ=255)
                                                                                                                                         aaGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTa    <  2:1526350/141‑1 (MQ=255)
                                                                                                                                           gCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATGCCTTTCAGCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAGATAAGGTAAt  <  2:63540/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAAATGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAT  >  CP000730/803388‑803667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: