Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 803,539 A→G 8.6% intergenic (+47/+31) USA300HOU_0750 → / ← dtpT hypothetical protein/POT family proton (H+)‑dependent oligopeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730803,5390AG 8.6% 432.3 / 23.4 151intergenic (+47/+31)USA300HOU_0750/dtpThypothetical protein/POT family proton (H+)‑dependent oligopeptide transporter
Reads supporting (aligned to +/- strand):  ref base A (62/76);  new base G (8/5);  total (70/81)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 151 total aligned reads displayed.

ATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGACCT  >  CP000730/803401‑803678
                                                                                                                                          |                                                                                                                                            
aTGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACt                                                                                                                                            <  2:201287/141‑1 (MQ=255)
 tGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACtt                                                                                                                                           <  2:27605/141‑1 (MQ=255)
  gAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTa                                                                                                                                          <  2:680716/141‑1 (MQ=255)
    aaTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACt                                                                                                                                        <  1:198659/141‑1 (MQ=255)
       tGATTTCTATAGAGTGGTACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtt                                                                                                                                    <  1:349054/141‑1 (MQ=255)
        gatttgaagagagGGGGACGAGAAAATAAAGTTAGAGTTAGCAGTAATTGGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGTATTTTATAAAAAAACTTTATACTATTTAAATTACTTACTGttt                                                                                                                                   >  2:829856/10‑141 (MQ=255)
        gATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTACTGttt                                                                                                                                   >  2:1427038/1‑141 (MQ=255)
        gATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGttt                                                                                                                                   <  2:28845/141‑1 (MQ=255)
         aTTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtttt                                                                                                                                  <  2:809437/141‑1 (MQ=255)
           ttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTACTGtttttt                                                                                                                                <  1:1427038/141‑1 (MQ=255)
              tataGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGaa                                                                                                                             <  2:1508379/141‑1 (MQ=255)
               ataGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAAt                                                                                                                            >  2:18140/1‑141 (MQ=255)
                      gggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCca                                                                                                                     >  2:617608/1‑141 (MQ=255)
                       ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                                    <  2:1612100/141‑1 (MQ=255)
                       ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                                    >  2:340016/1‑141 (MQ=255)
                       ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                                    <  2:1025345/141‑1 (MQ=255)
                        gACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                                   <  1:1641954/141‑1 (MQ=255)
                         aCGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaaca                                                                                                                  >  1:547537/1‑141 (MQ=255)
                              aaaaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATAtt                                                                                                             <  1:856890/141‑1 (MQ=255)
                              aaaaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATAtt                                                                                                             >  2:526169/1‑141 (MQ=255)
                              aaaaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATAtt                                                                                                             <  2:1112603/141‑1 (MQ=255)
                                aaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTaa                                                                                                           >  1:1219900/1‑141 (MQ=255)
                                aaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTaa                                                                                                           >  1:1311252/1‑141 (MQ=255)
                                 atatAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAAc                                                                                                          <  1:177229/141‑1 (MQ=255)
                                   ataGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCAAACAATATTAACGt                                                                                                        >  1:1510312/1‑141 (MQ=255)
                                    taGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGta                                                                                                       >  1:972333/1‑141 (MQ=255)
                                    taGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGta                                                                                                       >  1:972408/1‑141 (MQ=255)
                                      gTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGtata                                                                                                     >  2:471830/1‑141 (MQ=255)
                                          taGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCtt                                                                                                 <  1:5900/141‑1 (MQ=255)
                                            gCTGTCTATAATGAGCATATTAAGTTTTTTTTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTc                                                                                               >  2:1237456/1‑141 (MQ=255)
                                                     aaTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTCCTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtg                                                                                      <  1:417685/141‑1 (MQ=255)
                                                     aaTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtg                                                                                      <  1:1593169/141‑1 (MQ=255)
                                                      aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtga                                                                                     >  1:275831/1‑141 (MQ=255)
                                                      aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAAAGTATACCTTTCATCGCTTtga                                                                                     >  1:850420/1‑141 (MQ=255)
                                                        gAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtgatg                                                                                   <  2:207078/141‑1 (MQ=255)
                                                          gCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTgatgat                                                                                 <  1:1089207/141‑1 (MQ=255)
                                                           cATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGAtt                                                                                >  2:794617/1‑141 (MQ=255)
                                                               ttAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAg                                                                            <  2:1184422/141‑1 (MQ=255)
                                                                tAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAgg                                                                           <  1:1470282/141‑1 (MQ=255)
                                                                tAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAgg                                                                           <  2:285089/141‑1 (MQ=255)
                                                                tAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAgg                                                                           <  1:1470522/141‑1 (MQ=255)
                                                                   gTTTTTATTTATACTGATATCTTGAATTTAATTACTAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTga                                                                        <  1:1536249/141‑1 (MQ=255)
                                                                   gTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtga                                                                                     >  2:757494/1‑128 (MQ=255)
                                                                   gTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTga                                                                        <  2:1484150/141‑1 (MQ=255)
                                                                     ttttATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTgaga                                                                      <  1:651571/141‑1 (MQ=255)
                                                                     ttttATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTgaga                                                                      <  1:1025461/141‑1 (MQ=255)
                                                                        tatttttACTGTAATCTTAAAATTAATTAAGATAATCTTAGTAAAAAACAGTAAGGCATTTAATTTACTCACTGTTTTTTGAATTAGGCCAACAGTATTGGCGTATACCTTTTTTCTCTATGATGAGGAAATGCGTGATTg                                                                   >  2:1184260/1‑141 (MQ=255)
                                                                        tatttatACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTg                                                                   <  2:499472/141‑1 (MQ=255)
                                                                          tttatACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCt                                                                 <  1:75949/141‑1 (MQ=255)
                                                                             ataCTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAAt                                                              <  1:883754/141‑1 (MQ=255)
                                                                                 tGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACaa                                                          >  2:196685/1‑141 (MQ=255)
                                                                                  gATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAAt                                                         >  1:1004816/1‑141 (MQ=255)
                                                                                      tCTTGAATTTAATTAATAGAAACACCTAATGCAAATTTAGGGTATTTTAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAAttgtt                                                     >  2:494329/1‑141 (MQ=255)
                                                                                        ttGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATtgttgt                                                   >  1:789131/1‑141 (MQ=255)
                                                                                        ttGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGAAATCTAAATGACATACTGTATATTGAATTAGGCCAACAAAATTAACGTATACCTTTCATCGCTTTAATGATTAAAGGTAACATTGCTAATACAAGtgttgt                                                   >  2:417534/1‑141 (MQ=255)
                                                                                         tGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTa                                                  >  2:491193/1‑141 (MQ=255)
                                                                                          gactctaattattAGAAATCTGTAAAAAAACAGTAATCCATTTATATGACTTACTGCTTTTTGAATTAGGCCAAAAATATTAACGTATACCTTTCATCTCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTaa                                                 >  2:849285/6‑141 (MQ=255)
                                                                                           aaTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAAc                                                <  1:42722/141‑1 (MQ=255)
                                                                                            aTTTAATTAATAGAAACCTATCAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTCGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTagaa                                               >  2:354976/1‑138 (MQ=255)
                                                                                            aTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAAc                                                <  1:658191/141‑1 (MQ=255)
                                                                                            aTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACa                                               >  1:474295/1‑141 (MQ=255)
                                                                                            aTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACa                                               >  2:700582/1‑141 (MQ=255)
                                                                                               taattaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTATTGTTACaaca                                            <  1:544753/141‑3 (MQ=255)
                                                                                                aattaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataa                                           >  1:1049978/1‑141 (MQ=255)
                                                                                                aattaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACATATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataa                                           >  2:879555/1‑141 (MQ=255)
                                                                                                 attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                                          >  1:372209/1‑141 (MQ=255)
                                                                                                 attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                                          >  2:1496034/1‑141 (MQ=255)
                                                                                                 attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                                          >  1:1515734/1‑141 (MQ=255)
                                                                                                 attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                                          >  2:1304467/1‑141 (MQ=255)
                                                                                                      tAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTCCTGTTTTTTGAATTAGGCCAACAATATTAACGTATCCCTTTCAGCGCTTTGATGATCAAAAGCGAGATTGCTAATACAATTTTTGTACCAATAATCGcaa                                     >  2:292318/1‑141 (MQ=255)
                                                                                                      tAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaa                                     >  1:1612879/1‑141 (MQ=255)
                                                                                                           aCCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAacaaca                                <  2:404290/141‑1 (MQ=255)
                                                                                                              tataAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCt                             <  1:769130/141‑1 (MQ=255)
                                                                                                               ataAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTa                            >  2:677390/1‑141 (MQ=255)
                                                                                                                taAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAg                           <  1:112531/141‑1 (MQ=255)
                                                                                                                taAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAg                           <  1:519340/141‑1 (MQ=255)
                                                                                                                      aaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt                     >  1:1317024/1‑141 (MQ=255)
                                                                                                                       aCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                                   >  2:1445340/1‑46 (MQ=255)
                                                                                                                       aCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                                   <  1:1445340/46‑1 (MQ=255)
                                                                                                                       aCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                                   <  1:619048/46‑1 (MQ=255)
                                                                                                                       aCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                                   >  2:619048/1‑46 (MQ=255)
                                                                                                                          gTAAGTCATTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg                 >  1:1062601/1‑141 (MQ=255)
                                                                                                                          gTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg                 >  1:703822/1‑141 (MQ=255)
                                                                                                                           taagttaTTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTa                                                  <  1:1224260/101‑1 (MQ=255)
                                                                                                                           tAAGTCATTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTa                                                  >  2:1224260/1‑107 (MQ=255)
                                                                                                                           tAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGt                <  2:524283/141‑1 (MQ=255)
                                                                                                                            aaGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTa               <  1:1616725/141‑1 (MQ=255)
                                                                                                                            aaGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTa               <  2:1526350/141‑1 (MQ=255)
                                                                                                                                cATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAttt           >  1:336636/1‑141 (MQ=255)
                                                                                                                                 aTTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttg          >  1:1011406/1‑141 (MQ=255)
                                                                                                                                 aTTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttg          <  1:671262/141‑1 (MQ=255)
                                                                                                                                  tttAAATGGCTTACTGTTTTTTGAATTAGGCCAACCATATTAACGTATACTTTTCATCGGTTTGGTGTGTAAAGGTGGGTTTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttgt         >  1:746237/1‑141 (MQ=255)
                                                                                                                                  tttAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttgt         <  1:617608/141‑1 (MQ=255)
                                                                                                                                   ttAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttgtt        <  2:1004816/141‑1 (MQ=255)
                                                                                                                                    tAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAg                           >  1:1038623/1‑121 (MQ=255)
                                                                                                                                    tAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAg                           <  2:1038623/121‑1 (MQ=255)
                                                                                                                                    tAAATGACTTACTGTTTTTTTTTTTTTTTCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttgttt       >  2:292875/1‑141 (MQ=255)
                                                                                                                                    tAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttgttt       <  1:1574838/141‑1 (MQ=255)
                                                                                                                                        tGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGAcc   <  1:794617/141‑1 (MQ=255)
                                                                                                                                         gACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGACCt  <  2:1311748/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
ATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGACCT  >  CP000730/803401‑803678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: