Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,042,789 T→C 6.1% intergenic (+19/‑903) USA300HOU_0984 → / → USA300HOU_0985 glycosyltransferase/possible iron (Fe3+) ABC superfamily ATP binding cassette transporter, binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,042,7890TC 6.1% 326.3 / 10.2 116intergenic (+19/‑903)USA300HOU_0984/USA300HOU_0985glycosyltransferase/possible iron (Fe3+) ABC superfamily ATP binding cassette transporter, binding protein
Reads supporting (aligned to +/- strand):  ref base T (53/55);  new base C (3/4);  total (56/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01

Only 100 of 117 total aligned reads displayed.

TCAGCTTGTTAATAATGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCATTACGATATGTAT  >  CP000730/1042650‑1042927
                                                                                                                                           |                                                                                                                                          
tCAGCTTGTTAATAATGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTAtt                                                                                                                                           >  1:562337/1‑141 (MQ=255)
    cTTGTTAATAATGATGTATTAGCAGTGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAAAAAAATGGTTAAATCTTTTCAATAGATAAATGAAAAAATCCTAGGTACTCATg                                                                                                                                       <  1:676831/141‑1 (MQ=255)
     ttGTTAATAATGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGa                                                                                                                                      <  1:442177/141‑1 (MQ=255)
       gTTAATAATGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGt                                                                                                                                    <  1:1199503/141‑1 (MQ=255)
       gTTAATAATGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTACTCATGaat                                                                                                                                    <  1:810485/141‑3 (MQ=255)
        ttAATAATGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTa                                                                                                                                   <  1:125082/141‑1 (MQ=255)
        ttAATAATGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTa                                                                                                                                   >  1:302774/1‑141 (MQ=255)
          aataatGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTAcc                                                                                                                                 <  2:469196/141‑1 (MQ=255)
           ataatGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCt                                                                                                                                <  1:7644/141‑1 (MQ=255)
             aatGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAg                                                                                                                              >  1:936734/1‑141 (MQ=255)
                gatgTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGTAAAAATCCTAGGTACTCATGAATACCTAGGAt                                                                                                                           <  1:1105699/141‑1 (MQ=255)
                   gTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGAtttt                                                                                                                        >  1:1231814/1‑141 (MQ=255)
                     aTTAGCGGCGAATTTTGGTTGAAAAGCGGGAGAAAACATTATAGAAAAATATTCAACGGAATCAAAATAAGAAAAAGGGTTAATTCTTTTCAATGGCTAATTGAAAAAATCTTAGGTTTTCATGAGTACCTAGGTTTTTTa                                                                                                                      <  2:1079917/141‑1 (MQ=255)
                     aTTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAGAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTa                                                                                                                      >  2:237959/1‑141 (MQ=255)
                     aTTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTa                                                                                                                      >  1:1491192/1‑141 (MQ=255)
                      ttAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTAc                                                                                                                     >  1:1054238/1‑141 (MQ=255)
                      ttAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTAc                                                                                                                     <  1:1330321/141‑1 (MQ=255)
                      ttAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTAACTAAGATTTTTaa                                                                                                                     >  2:38828/1‑140 (MQ=255)
                        agcagcGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGc                                                                                                                   >  2:460181/1‑141 (MQ=255)
                        agcagcGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGc                                                                                                                   >  1:1079917/1‑141 (MQ=255)
                             cGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTACTCATg                                                                                                                                       >  2:1540306/1‑116 (MQ=255)
                              ggAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTAcatttagct                                                                                                             >  2:314514/1‑133 (MQ=255)
                              ggAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATa                                                                                                             >  2:70497/1‑141 (MQ=255)
                              ggAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATa                                                                                                             >  1:504687/1‑141 (MQ=255)
                               gAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTAcatttagcta                                                                                                            <  1:314514/141‑10 (MQ=255)
                                 gTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAAt                                                                                                          <  1:1621645/141‑1 (MQ=255)
                                 gTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAAt                                                                                                          <  1:274290/141‑1 (MQ=255)
                                 gTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAAt                                                                                                          <  1:426906/141‑1 (MQ=255)
                                  tttGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATaaa                                                                                                           <  2:824739/139‑1 (MQ=255)
                                  tttGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATaaa                                                                                                           >  1:824739/1‑139 (MQ=255)
                                  tttGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATa                                                                                                         >  2:1601357/1‑141 (MQ=255)
                                  tttGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATa                                                                                                         >  1:51787/1‑141 (MQ=255)
                                  ttgggTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATa                                                                                                         <  2:467014/138‑1 (MQ=255)
                                   ttGGTTCAAAAGCGAGAGAAAACATTATATAAAAACATTCAACCGAATCAATATTAGAAAAATGGTTATATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACTCATGATAAATAc                                                                                                        <  2:1471072/141‑1 (MQ=255)
                                     ggTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACaa                                                                                                      <  1:774509/141‑1 (MQ=255)
                                     ggTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAAAAAATAAAAAAAAAATGGTGAAATCGTTTCAATAGCTAAATGAACAAAACCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGAaaaaaaaaa                                                                                                      <  2:512354/141‑6 (MQ=255)
                                        tcgcatgagacagaCAACATTATCGAAAAATATTCACCAGAATCAAAATTAGCAGAGTGGTTGAATTTTTTCAATAGCTAAATGAAACAAGCCTAGGGATTCATGAGGACCTAGGATTTTTACGCATGATAAATAaaaaaa                                                                                                   <  2:968755/130‑1 (MQ=255)
                                          gAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACaaaaaaa                                                                                                 <  2:1041998/141‑1 (MQ=255)
                                               cGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTAACTAGGATTTTTACGCATGATAAATACAAAAAAagatag                                                                                            >  2:854529/1‑141 (MQ=255)
                                                gagagaAAACATTATAGAAAAATATTCAACGGAATAAATATTGGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAgg                                                                                           >  1:1426605/1‑141 (MQ=255)
                                                    gaAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAg                                                                                       >  1:122448/1‑141 (MQ=255)
                                                     aaaaCATAATAGAAAAATATAAAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGAAAAAAAAAAAAAAAAAAAGAGAGGATAGt                                                                                      <  2:1384077/141‑1 (MQ=255)
                                                      aaaCATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTc                                                                                     <  1:204512/141‑1 (MQ=255)
                                                        aCATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGACAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATCCAAAAAAAGAGAGGATAGTCct                                                                                   >  1:493284/1‑141 (MQ=255)
                                                        aCATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGactatcct                                                                                   <  1:945284/141‑8 (MQ=255)
                                                        aCATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCct                                                                                   >  2:540528/1‑141 (MQ=255)
                                                        aCATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAACGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTAAGCATGATAAATACAAAAAAAGAGAGGATAGTCct                                                                                   >  2:1046319/1‑141 (MQ=255)
                                                          aTTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCctct                                                                                 >  1:1560844/1‑141 (MQ=255)
                                                          aTTAAAGAAAAAGATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCctct                                                                                 <  2:1086062/141‑1 (MQ=255)
                                                           ttATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTATGTATTCATGAGTACCTAGGATTTTTACGCATGATAAAAACAAAAAAAGAGAGGATAGTCCtctc                                                                                <  2:1640025/141‑1 (MQ=255)
                                                           ttATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGactatcctctc                                                                                <  1:1140034/141‑11 (MQ=255)
                                                           aaaaaGAAAAATATTAAACAGAATCAAAATAAGAAAAAAGGTTAAATCATTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACACAAAAAAAAAAAAAAAAAAGAGAGGATAGTCCtctc                                                                                <  2:199514/137‑1 (MQ=255)
                                                              taGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCaaa                                                                             <  2:873718/141‑1 (MQ=255)
                                                              taGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTAAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGCTTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCaaa                                                                             >  1:795968/1‑141 (MQ=255)
                                                              taGAAAAAAACATAACGGAATCAATATTAGAAAAATGGGTAAACCATTGCAATAGCTAAAAGAAAAAAACATAGGTATTCATGAATACCTACGATTATTACGCGCGATAAAGACAAAAAAAGATAGGATAGTCCACTCcaa                                                                             >  2:591839/1‑141 (MQ=255)
                                                              aaaaaaaaaaaTCAACGAAATCAATATTAAAAAAATGGATAAATATTTACAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCAAAAAAAAAAAAAAAAAAGAGAGGATAGTCCTCTCaaa                                                                             <  2:986990/138‑1 (MQ=255)
                                                               aGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAAc                                                                            >  2:1450986/1‑141 (MQ=255)
                                                                   aaaTATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATa                                                                        >  1:533691/1‑141 (MQ=255)
                                                                     atatTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGt                                                                      <  1:1252739/141‑1 (MQ=255)
                                                                       atTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTta                                                                    >  2:317254/1‑141 (MQ=255)
                                                                       atTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTta                                                                    >  1:787472/1‑141 (MQ=255)
                                                                       atTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTta                                                                    >  2:825550/1‑141 (MQ=255)
                                                                         tCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTtaat                                                                  <  1:254354/141‑1 (MQ=255)
                                                                          cAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTtaata                                                                 <  1:1225600/141‑1 (MQ=255)
                                                                          aaaCGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATAATAAAAAAAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTtaata                                                                 <  2:782853/140‑1 (MQ=255)
                                                                           aaCGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCACGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGNGGATAGTCCTCTNAAACAATAGTtaataa                                                                <  1:1374555/141‑1 (MQ=255)
                                                                            aCGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATaaa                                                               >  2:1017086/1‑141 (MQ=255)
                                                                                 aTCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGt                                                          <  1:165464/141‑1 (MQ=255)
                                                                                    aaTATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTAGGCATGATAACTACAAAAAAAGAGAGGAAAGTCCTCTCAAACAATAGTCAATAAAATTGTAGc                                                       >  2:1290899/1‑141 (MQ=255)
                                                                                    aaTATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCAAAAAAAAAAAAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGc                                                       <  2:974183/141‑1 (MQ=255)
                                                                                     atatTAGATAAATGGTTAAATCTTTTCAATAGCTAAATGAAATAATCCTAGGTATTCATGAGTAACTAGGATTTTTACGAAAAAAAAAAAAAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGcc                                                      <  2:1582438/141‑1 (MQ=255)
                                                                                       atTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAGAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCAt                                                    <  1:105747/141‑1 (MQ=255)
                                                                                       atTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCAt                                                    <  1:1017086/141‑1 (MQ=255)
                                                                                             aaaaTGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATttcttc                                              >  2:1414765/1‑141 (MQ=255)
                                                                                                aTGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAg                                           >  1:127168/1‑141 (MQ=255)
                                                                                                  ggTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCt                                         >  1:1625681/1‑141 (MQ=255)
                                                                                                  ggTTAAATCTTGTATATATCTAAATGAAAAAATCCTAGGTATACATGAATACCTAGGATTTTTACGAATAAAAAAAAAAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCt                                         <  2:1556532/141‑1 (MQ=255)
                                                                                                    ttAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGt                                       <  1:381548/141‑1 (MQ=255)
                                                                                                    ttAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGt                                       >  1:677301/1‑141 (MQ=255)
                                                                                                         tCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAacta                                  <  1:200884/141‑1 (MQ=255)
                                                                                                             ttCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTaa                              >  1:1425921/1‑141 (MQ=255)
                                                                                                              tgaaTAANTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGAAAAATAAAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAAt                             <  2:1246278/139‑1 (MQ=255)
                                                                                                              tCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATAAAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAAt                             <  2:690582/141‑1 (MQ=255)
                                                                                                                    gCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGc                       >  1:1198928/1‑141 (MQ=255)
                                                                                                                      tAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTa                     <  1:1371873/141‑1 (MQ=255)
                                                                                                                         aTGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATATATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATCCAAGCTAAGt                  >  1:1250844/1‑141 (MQ=255)
                                                                                                                         aTGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGt                  <  1:706635/141‑1 (MQ=255)
                                                                                                                         aTGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGt                  >  1:1250858/1‑141 (MQ=255)
                                                                                                                             aaaaaTCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCAt              <  2:1231814/141‑1 (MQ=255)
                                                                                                                              aaaaTCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCAtt             >  1:1096924/1‑141 (MQ=255)
                                                                                                                              aaaaTCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCAtt             >  2:1495582/1‑141 (MQ=255)
                                                                                                                              aaaaTCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCAtt             >  2:1014152/1‑141 (MQ=255)
                                                                                                                              aaaaTCCTAGGTATTCATGAGGACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAAAGGATAGTCCTCTCAAACAATAGTTAATAAAATAGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCAtt             >  2:1418962/1‑141 (MQ=255)
                                                                                                                                aaTCCTAGGTATTCATGAGTACCTAGGATTTTTACGCACGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCATTAc           >  2:291994/1‑141 (MQ=255)
                                                                                                                                 aTCCTAGGTATTCACGAGTACGTAGGAATTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCCCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCAAGCTGTAACTACTAATACAAGCTACGTTCATTACg          >  2:1366227/1‑141 (MQ=255)
                                                                                                                                  tCCTAGGTATTTATTAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTGTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCATTACGa         <  1:945831/141‑1 (MQ=255)
                                                                                                                                    cTAGGTACTCATGAATACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCATTACGata       >  1:1131309/1‑141 (MQ=255)
                                                                                                                                     tAGGTACTCATGAATACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCATTACGatat      >  1:802689/1‑141 (MQ=255)
                                                                                                                                      aGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCATTACGAtatg     >  2:245977/1‑141 (MQ=255)
                                                                                                                                         tATTCATGAGTACCTAGGATTTTTACTCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCATTACGAtatgtat  <  2:1303054/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCAGCTTGTTAATAATGATGTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACGGAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAAATGAAAAAATCCTAGGTATTCATGAGTACCTAGGATTTTTACGCATGATAAATACAAAAAAAGAGAGGATAGTCCTCTCAAACAATAGTTAATAAAATTGTAGCCATTTCTTCCAGCTGTAACTACTAATACAAGCTAAGTTCATTACGATATGTAT  >  CP000730/1042650‑1042927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: